Great research starts with great data.

Learn More
More >
Patent Analysis of

Quantification of adaptive immune cell genomes in a complex mixture of cells

Updated Time 12 June 2019

Patent Registration Data

Publication Number

US10150996

Application Number

US15/304631

Application Date

05 March 2015

Publication Date

11 December 2018

Current Assignee

FRED HUTCHINSON CANCER RESEARCH CENTER,ADAPTIVE BIOTECHNOLOGIES CORP.

Original Assignee (Applicant)

ADAPTIVE BIOTECHNOLOGIES CORPORATION

International Classification

C12Q1/68,C12Q1/6881

Cooperative Classification

C12Q1/6881,C12Q2600/16,C12Q2600/156

Inventor

ROBINS, HARLAN SAUL,CARLSON, CHRISTOPHER SCOTT,RIEDER, MARK J.,SHERWOOD, ANNA M.,EMERSON, RYAN O.

Patent Images

This patent contains figures and images illustrating the invention and its embodiment.

US10150996 Quantification adaptive immune cell 1 US10150996 Quantification adaptive immune cell 2 US10150996 Quantification adaptive immune cell 3
See all images <>

Abstract

A relative representation of adaptive immune cells in a biological sample is quantified using multiplex PCR and sequencing of adaptive immune cells, control genes, and synthetic template molecules. Disclosed herein are methods for quantifying a number of adaptive immune cells in a biological sample, and methods for quantifying a relative representation of adaptive immune cells in a biological sample that comprises a mixture of cells comprising adaptive immune cells and cells that are not adaptive immune cells. Methods are provided for amplifying by multiplex PCR and sequencing a first set of synthetic templates each comprising one TCR or IgV segment and one TCR of Ig J or C segment and a unique bar code.

Read more

Claims

1. A method for determining a ratio of T cells or B cells in a sample relative to the total number of input genomes contained in said sample comprising:

A) amplifying by multiplex PCR and sequencing:

i) rearranged CDR3 oligonucleotide sequences from T cell receptor (TCR) loci from T cells or Immunoglobulin (Ig) loci from B cells in said sample to obtain a total number of output biological sequences, each oligonucleotide sequence comprising a V segment and a J segment; ii) a first set of synthetic templates representing substantially all possible V segment and J segment combinations and each comprising one TCR or Ig V segment and one TCR or Ig J or C segment and a unique barcode which identifies said synthetic template as synthetic, and wherein each synthetic template comprises a unique combination of a V segment and J or C segment; B) determining an amplification factor for each synthetic template comprising a unique combination of a V segment and a J or C segment, wherein said amplification factor is represented by a total number of first synthetic templates observed from step A(ii) divided by a total input number of unique first synthetic templates input in step A(ii); C) determining the total number of T cells or B cells in the sample by dividing the total number of output biological sequences observed in step A(i) by the amplification factor from step B; D) amplifying by multiplex PCR and sequencing: i) one or more genomic control regions from DNA obtained from said sample to obtain a total number of output biological sequences for each genomic control region; and ii) a second set of synthetic templates comprising the sequence of one or more of said genomic control sequences, a unique barcode and a stretch of random nucleic acids, wherein each synthetic template is represented only once; E) determining an amplification factor for each of said genomic control region by dividing the total number of second synthetic templates amplified and sequenced in step D(ii) by the total input number of unique second synthetic templates amplified and sequenced in step D(ii); F) determining the total number of input genomes by dividing the total number of output biological sequences for each genomic control region from step D(i) by the corresponding amplification factor for that genomic control region from Step E; and G) determining the ratio of T cells or B cells contained in the sample relative to the number of total genomes in the sample by dividing the total number of T cells or B cells obtained in step C by the total number of input genomes obtained in step F.

2. The method of claim 1, wherein the first set of synthetic templates comprises the sequence of formula I: 5′-U1-B1-V-B2-J-B3-U2-3′, wherein

A) V is an oligonucleotide sequence comprising at least 20 and not more than 1000 contiguous nucleotides of a TCR or Ig variable (V) region encoding gene sequence, or the complement thereof and each template in set first set of synthetic templates having a unique V-region oligonucleotide sequence; B) J is an oligonucleotide sequence comprising at least 15 and not more than 600 contiguous nucleotides of a TCR or Ig joining (J) region encoding gene sequence, or the complement thereof and each template in said first set of synthetic templates comprising a unique J-region oligonucleotide sequence; C) U1 comprises an oligonucleotide sequence that is selected from (i) a first universal adaptor oligonucleotide sequence; and (ii) a first sequencing platform oligonucleotide sequence that is linked to and positioned 5′ to a first universal adaptor oligonucleotide sequence; D) U2 comprises an oligonucleotide sequence that is selected from (i) a second universal adaptor oligonucleotide sequence; and (ii) a second sequencing platform oligonucleotide sequence that is linked to and positioned 5′ to a second universal adaptor oligonucleotide sequence; and E) B1, B2 and B3 each independently comprise either nothing or an oligonucleotide barcode sequence of 3-25 nucleic acids that uniquely identifies, as a pair combination (i) said unique V-region oligonucleotide sequence; and said unique J-region oligonucleotide, wherein at least one of B1, B2 and B3 is present in each synthetic template contained in said first set of oligonucleotides.

3. The method of claim 2, wherein each of the synthetic templates contained in said first set of synthetic templates further comprises a stretch of unique random nucleotides.

4. The method of claim 3, wherein the random stretch of nucleotides comprise from 4 to 50 nucleotides, or wherein the random stretch of nucleotides comprises 8 nucleotides.

5. The method of claim 1, wherein the total number of synthetic templates in said first set of synthetic templates subject to amplification in step A(ii) is determined using a limiting dilution of said synthetic templates each comprising a unique TCR or Ig V and J or C region such that the number of observed unique synthetic templates allows inference of the total number of input synthetic template molecules.

6. The method of claim 3, wherein the total number of synthetic templates in said first set of synthetic templates subject to amplification in step A(ii) is determined by counting the number of unique synthetic templates based on the unique random nucleotides contained in each synthetic template.

7. The method of claim 1, comprising amplifying by multiplex PCR and sequencing two or more, or three or more, or four or more, or five or more genomic control regions in step D(i).

8. The method of claim 1, comprising amplifying by multiplex PCR and sequencing five genomic control regions in step D(i), and wherein each of the five genomic control regions has a predictable copy number.

9. The method of claim 1, wherein the one or more genomic control regions are selected from the group consisting of ACTB, B2M, C1orf34, CHMP2A, GPI, GUSB, HMBS, HPRT1, PSMB4, RPL13A, RPLP0, SDHA, SNRPD3, UBC, VCP, VSP29, PPIA, PSMB2, RAB7A, REEP5, and EMC7, or wherein the one or more genomic control regions are PSMB2, RAB7A, PPIA, REEP5, and EMC7.

10. The method of claim 8, wherein amplification factors are determined for each of said five genomic control regions in step E.

11. The method of claim 10, wherein the total number of input genomes is calculated in step F by taking an average using each of the five amplification factors determined for each of said five genomic control regions.

12. The method of claim 1, wherein the amplification by multiplex PCR in steps A and D are done in a single multiplex reaction.

13. The method of claim 1, wherein the amplification by multiplex PCR in step A or step D is performed using a plurality of oligonucleotide primer sets comprising:

A) a plurality of V segment oligonucleotide primers that are each independently capable of specifically hybridizing to at least one polynucleotide encoding a TCR of Ig V region polypeptide or to the complement thereof, wherein each V segment primer comprises a nucleotide sequence of at least 15 contiguous nucleotides that is complementary to at least one functional a TCR or Ig V region encoding gene segment and wherein said plurality of V segment primers specifically hybridize to substantially all functional TCR or Ig V region encoding gene segments that are present in the composition, and B) a plurality of J segment oligonucleotide primers that are each independently capable of specifically hybridizing to at least one polynucleotide encoding an TCR or Ig J region polypeptide or to the complement thereof, wherein each J segment primer comprises a nucleotide sequence of at least 15 contiguous nucleotides that is complementary to at least one functional TCR or IG J region encoding gene segment and wherein said plurality of J segment primers specifically hybridize to substantially all functional TCR or IG J region encoding gene segments that are present in the composition.

14. The method of claim 13, wherein either or both of:

(i) said plurality of V segment oligonucleotide primers comprise sequences having at least 90% sequence identity to nucleotide sequences set forth in SEQ ID NOs: 1-120, 147-158, 167-276, 407-578, 593-740, and(ii) said plurality of J segment oligonucleotide primers comprise sequences having at least 90% sequence identity to nucleotide sequences set forth in SEQ ID NOs: 121-146, 159-166, 277-406, 579-592, 741-764.

15. The method of claim 1, wherein said sample is fresh tissue, frozen tissue, or fixed tissue.

16. The method of claim 1, wherein said TCR V segment comprises a TCRG V segment, a TCRD V segment, a TCRA V segment, or a TCRB V segment.

17. The method of claim 1, wherein said TCR J segment comprises a TCRD J segment, a TCRG J segment, a TCRA J segment, or a TCRB J segment.

18. The method of claim 1, wherein said Ig V segment comprises an IGH V gene segment, an IGL V gene segment, or an IGK V gene segment.

19. The method of claim 1, wherein said Ig J region segment comprises an IGH J gene segment, an IGL J gene segment, or an IGK V gene segment.

20. The method of claim 1 wherein said output sequences obtained in step A(i), said synthetic templates contained in said first set of synthetic templates in step A(ii), said output sequences for each genomic control region in step D(i) and said synthetic templates contained in said second set of synthetic templates in step D(ii) are each about 100-300 nucleotides in length.

Read more

Claim Tree

  • 1
    1. A method for determining a ratio of T cells or B cells in a sample relative to the total number of input genomes contained in said sample comprising:
    • A) amplifying by multiplex PCR and sequencing: i) rearranged CDR3 oligonucleotide sequences from T cell receptor (TCR) loci from T cells or Immunoglobulin (Ig) loci from B cells in said sample to obtain a total number of output biological sequences, each oligonucleotide sequence comprising a V segment and a J segment; ii) a first set of synthetic templates representing substantially all possible V segment and J segment combinations and each comprising one TCR or Ig V segment and one TCR or Ig J or C segment and a unique barcode which identifies said synthetic template as synthetic, and wherein each synthetic template comprises a unique combination of a V segment and J or C segment;
    • B) determining an amplification factor for each synthetic template comprising a unique combination of a V segment and a J or C segment, wherein said amplification factor is represented by a total number of first synthetic templates observed from step A(ii) divided by a total input number of unique first synthetic templates input in step A(ii);
    • C) determining the total number of T cells or B cells in the sample by dividing the total number of output biological sequences observed in step A(i) by the amplification factor from step B;
    • D) amplifying by multiplex PCR and sequencing: i) one or more genomic control regions from DNA obtained from said sample to obtain a total number of output biological sequences for each genomic control region; and ii) a second set of synthetic templates comprising the sequence of one or more of said genomic control sequences, a unique barcode and a stretch of random nucleic acids, wherein each synthetic template is represented only once;
    • E) determining an amplification factor for each of said genomic control region by dividing the total number of second synthetic templates amplified and sequenced in step D(ii) by the total input number of unique second synthetic templates amplified and sequenced in step D(ii);
    • F) determining the total number of input genomes by dividing the total number of output biological sequences for each genomic control region from step D(i) by the corresponding amplification factor for that genomic control region from Step E; and
    • G) determining the ratio of T cells or B cells contained in the sample relative to the number of total genomes in the sample by dividing the total number of T cells or B cells obtained in step C by the total number of input genomes obtained in step F.
    • 2. The method of claim 1, wherein
      • the first set of synthetic templates comprises the sequence of formula I: 5′-U1-B1-V-B2-J-B3-U2-3′, wherein
    • 5. The method of claim 1, wherein
      • the total number of synthetic templates in said first set of synthetic templates subject to amplification in step A(ii) is determined using a limiting dilution of said synthetic templates each comprising
    • 7. The method of claim 1, comprising
      • amplifying by multiplex PCR and sequencing two or more, or three or more, or four or more, or five or more genomic control regions in step D(i).
    • 8. The method of claim 1, comprising
      • amplifying by multiplex PCR and sequencing five genomic control regions in step D(i), and wherein each of the five genomic control regions has a predictable copy number.
    • 9. The method of claim 1, wherein
      • the one or more genomic control regions are selected from the group consisting of
    • 12. The method of claim 1, wherein
      • the amplification by multiplex PCR in steps A and D are done in a single multiplex reaction.
    • 13. The method of claim 1, wherein
      • the amplification by multiplex PCR in step A or step D is performed using a plurality of oligonucleotide primer sets comprising:
    • 15. The method of claim 1, wherein
      • said sample is fresh tissue, frozen tissue, or fixed tissue.
    • 16. The method of claim 1, wherein
      • said TCR V segment comprises
    • 17. The method of claim 1, wherein
      • said TCR J segment comprises
    • 18. The method of claim 1, wherein
      • said Ig V segment comprises
    • 19. The method of claim 1, wherein
      • said Ig J region segment comprises
    • 20. The method of claim 1 wherein
      • said output sequences obtained in step A(i), said synthetic templates contained in said first set of synthetic templates in step A(ii), said output sequences for each genomic control region in step D(i) and said synthetic templates contained in said second set of synthetic templates in step D(ii) are each about 100-300 nucleotides in length.
See all independent claims <>

Description

SEQUENCE LISTING

The Sequence Listing associated with this application is provided in text format in lieu of a paper copy, and is hereby incorporated by reference into the specification. The name of the text file containing the Sequence Listing is 26341 US_CRF_sequencelisting.txt. This text file was created on Mar. 5, 2015, 2014, is about 2.08 MB in size, and is being submitted electronically via EFS-Web.

BACKGROUND

The adaptive immune system protects higher organisms against infections and other pathological events that may be attributable to foreign substances, using adaptive immune receptors, the antigen-specific recognition proteins that are expressed by hematopoietic cells of the lymphoid lineage and that are capable of distinguishing self from non-self molecules in the host. These lymphocytes may be found in the circulation and tissues of a host, and their recirculation between blood and the lymphatics has been described, including their extravasation via lymph node high endothelial venules, as well as at sites of infection, inflammation, tissue injury and other clinical insults. (See, e.g., Stein et al., 2005 Immunol. 116:1-12; DeNucci et al., 2009 Crit. Rev. Immunol. 29:87-109; Marelli-Berg et al., 2010 Immunol. 130:158; Ward et al., 2009 Biochem. J. 418:13; Gonzalez et al., 2011 Ann. Rev. Immunol. 29:215; Kehrl et al., 2009 Curr. Top. Microb. Immunol. 334:107; Steinmetz et al., 2009 Front. Biosci. (Schol. Ed.) 1:13.)

Accordingly, the dynamic nature of movement by lymphocytes throughout a host organism is reflected in changes in the qualitative (e.g., antigen-specificity of the clonally expressed adaptive immune receptor (immunoglobulin or T cell receptor), T cell versus B cell, T helper (Th) cell versus T regulatory (Treg) cell, effector T cell versus memory T cell, etc.) and quantitative distribution of lymphocytes among tissues, as a function of changes in host immune status.

For example, numerous studies have found an association between (i) the presence of tumor infiltrating lymphocytes (TIL) in a variety of solid tumors and (ii) patient prognosis and overall survival rates. In some studies, tumor infiltrating T cells having a specific phenotype (e.g., CD8+ and CD4+ T cells or regulatory T cells) are positive or negative predictors of survival (e.g., Jochems et al., 2011 Experimental Biol. Med. 236:567-579). In certain cases, however, TIL count alone is a predictor of long-term survival (e.g., Katz et al., 2009 Ann. Surg. Oncol. 16:2524-2530). Thus, quantitative determination of TIL counts has high prognostic value in a variety of cancers including colorectal, hepatocellular, gallbladder, pancreatic, esophageal, ovarian endometrial, cervical, bladder and urothelial cancers. While more is known about the association of tumor-infiltrating T cells, B cells are also known to infiltrate tumors and studies have shown an association of tumor-infiltrating B cells with survival advantage (e.g., Ladányi, et al., Cancer Immunol. Immunother. 60(12):1729-38, Jul. 21, 2011 (epub ahead of print).

The quantitative determination of the presence of adaptive immune cells (e.g., T and B lymphocytes) in diseased tissues may therefore provide useful information for diagnostic, prognostic and other purposes, such as in cancer, infection, inflammation, tissue injury and other conditions.

The adaptive immune system employs several strategies to generate a repertoire of T- and B-cell antigen receptors with sufficient diversity to recognize the universe of potential pathogens. B lymphocytes mature to express antibodies (immunoglobulins, Igs) that occur as heterodimers of a heavy (H) a light (L) chain polypeptide, while T lymphocytes express heterodimeric T cell receptors (TCR). The ability of T cells to recognize the universe of antigens associated with various cancers or infectious organisms is conferred by its T cell antigen receptor (TCR), which is made up of both an α (alpha) chain and a β (beta) chain or a γ (gamma) and a δ (delta) chain. The proteins which make up these chains are encoded by DNA, which employs a unique mechanism for generating the tremendous diversity of the TCR. This multi-subunit immune recognition receptor associates with the CD3 complex and binds to peptides presented by the major histocompatibility complex (MHC) class I and II proteins on the surface of antigen-presenting cells (APCs). Binding of TCR to the antigenic peptide on the APC is the central event in T cell activation, which occurs at an immunological synapse at the point of contact between the T cell and the APC.

Each TCR peptide contains variable complementarity determining regions (CDRs), as well as framework regions (FRs) and a constant region. The sequence diversity of αβ T cells is largely determined by the amino acid sequence of the third complementarity-determining region (CDR3) loops of the α and β chain variable domains, which diversity is a result of recombination between variable (Vβ), diversity (Dβ), and joining (Jβ) gene segments in the β chain locus, and between analogous Vα and Jα gene segments in the α chain locus, respectively. The existence of multiple such gene segments in the TCR α and β chain loci allows for a large number of distinct CDR3 sequences to be encoded. CDR3 sequence diversity is further increased by independent addition and deletion of nucleotides at the Vβ-Dβ, Dβ-Jβ, and Vα-Jα junctions during the process of TCR gene rearrangement. In this respect, immunocompetence is reflected in the diversity of TCRs.

The γδ TCR is distinctive from the αβ TCR in that it encodes a receptor that interacts closely with the innate immune system. TCRγδ, is expressed early in development, has specialized anatomical distribution, has unique pathogen and small-molecule specificities, and has a broad spectrum of innate and adaptive cellular interactions. A biased pattern of TCRγ V and J segment expression is established early in ontogeny as the restricted subsets of TCRγδ cells populate the mouth, skin, gut, vagina, and lungs prenatally. Consequently, the diverse TCRγ repertoire in adult tissues is the result of extensive peripheral expansion following stimulation by environmental exposure to pathogens and toxic molecules.

Igs expressed by B cells are proteins consisting of four polypeptide chains, two heavy chains (H chains) and two light chains (L chains), forming an H2L2 structure. Each pair of H and L chains contains a hypervariable domain, consisting of a VL and a VH region, and a constant domain. The H chains of Igs are of several types, μ, δ, γ, α, and β. The diversity of Igs within an individual is mainly determined by the hypervariable domain. Similar to the TCR, the V domain of H chains is created by the combinatorial joining of the VH, DH, and JH gene segments. Hypervariable domain sequence diversity is further increased by independent addition and deletion of nucleotides at the VH-DH, DH-JH, and VH-JH junctions during the process of Ig gene rearrangement. In this respect, immunocompetence is reflected in the diversity of Igs.

Quantitative characterization of adaptive immune cells based on the presence in such cells of functionally rearranged Ig and TCR encoding genes that direct productive expression of adaptive immune receptors has been achieved using biological samples from which adaptive immune cells can be readily isolated in significant numbers, such as blood, lymph or other biological fluids. In these samples, adaptive immune cells occur as particles in fluid suspension. See, e.g., US 2010/0330571; see also, e.g., Murphy, Janeway's Immunobiology (8th Ed.), 2011 Garland Science, NY, Appendix I, pp. 717-762. Previous methods include quantification of the relative representation of adaptive immune cells in a sample by amplifying V-region polypeptides, J-region polypeptides, and an internal control gene from the sample, and comparing the number of cells containing V- and J-region polypeptides to the number of cells containing the internal control gene. See, e.g., U.S. Ser. No. 13/656,265. However, this method does not allow for absolute quantitation of the adaptive immune cells in the sample. Although a relative representation of the adaptive immune cells can be determined, current methods do not allow determination of the absolute number of adaptive immune cells in the input sample.

There is a need for a method that permits accurate absolute quantitation of adaptive immune cells in a complex biological sample. There is also a need for an improved method for quantifying a relative representation of adaptive immune cells in such a complex biological sample.

SUMMARY OF INVENTION

Disclosed herein are methods for quantifying a number of adaptive immune cells in a biological sample, and methods for quantifying a relative representation of adaptive immune cells in a biological sample that comprises a mixture of cells comprising adaptive immune cells and cells that are not adaptive immune cells.

In one embodiment, a method is provided for determining the ratio of T or B cells in a sample relative to the total number of input genomes. In one embodiment, the method provides amplifying by multiplex PCR and sequencing rearranged Tcell receptor loci (TCRs) from T cells or immunoglobulin (Ig) loci from V cells in a sample to obtain a total number of output biological sequences. In a further embodiment, methods are provided for amplifying by multiplex PCR and sequencing a first set of synthetic templates each comprising one TCR or Ig V segment and one TCR of Ig J or C segment and a unique bar code which identifies said synthetic template as synthetic. In one embodiment, each synthetic template comprises a unique combination of V and J or C segments. In a further embodiment the method provides determining an amplification factor that is represented by the total number of first synthetic templates amplified and sequenced divided by the total input number of unique first synthetic templates. In one embodiment, the method provides for determining the total number of T cells or B cells in the sample by dividing the total number of output biological sequences by the amplification factor.

In one embodiment the method further provides amplifying by multiplex PCR and sequencing one or more genomic control regions from DNA obtained from a sample to obtain the total number of output biological sequences for each genomic control region. In a further embodiment methods are provided for amplifying by multiplex PCR and sequencing a second set of synthetic templates, each comprising the sequence of one or more of said genomic control regions, a unique barcode and stretch of random nucleic acids. In one embodiment each synthetic template in the second set of synthetic templates is represented only once. In a further embodiment, the method provides for determining an amplification factor for each of the one or more genomic control regions by dividing the total number of second synthetic templates amplified and sequenced by the total input number of unique second synthetic templates. In one embodiment, the method further provides for a method for determining the total number of input genomes by dividing the total number of output biological sequences from each genomic control region by the corresponding amplification factor for that genomic control region. In yet another embodiment the methods provide for determining the ratio of T cells or B cells contained in the sample relative to the total genomes by dividing the total number to T cells or B cells in the sample by the total number of input genomes in the sample.

In one embodiment, the sample is obtained from a mammalian subject. In another embodiment, the sample comprises a mixture of cells comprising T cells and/or B cells and cells that are not Tc ells and/or B cells.

In one embodiment the method includes synthetic templates which comprise the sequence, 5′-U1-B1-V-B2-J-B3-U2-3′. In one embodiment, V is an oligonucleotide sequence comprising at least 20 and not more than 1000 contiguous nucleotides of a TCR or Ig variable (V) region encoding gene sequence or the complement thereof. In one embodiment, each synthetic template comprises a unique V region oligonucleotide sequence. In one embodiment, J is an oligonucleotide sequence comprising at least 15 and not more than 600 contiguous nucleotides of a TCR or Ig joining (J) region encoding gene sequence or the complement thereof. In one embodiment, U1 comprises an oligonucleotide sequence that is a first universal adaptor sequence or a first sequencing platform oligonucleotide sequence that is linked to and positioned 5′ to a first universal adaptor oligonucleotide sequence. In a further embodiment, U2 comprises an oligonucleotide sequence that is a universal adaptor sequence or a second sequencing platform oligonucleotide sequence that is linked to and positioned 5′ to a second universal adaptor oligonucleotide sequence. In one embodiment, B1, B2 and B3 are each independently, either nothing or an oligonucleotide barcode sequence of 3-25 nucleic acids that uniquely identifies as a pair combination a unique V region oligonucleotide sequence and a unique J region oligonucleotide. In one embodiment at least one B1, B2 and B3 is present in each synthetic template. In one embodiment at least two of B1, B2 and B3 are present in each synthetic template. In one embodiment all three of B1, B2 and B3 are present in each synthetic template. In one embodiment, the synthetic templates further comprise a string of random oligonucleotides (randomers). In one embodiment, the string of random oligonucleotides comprises about 4 to about 50 nucleotides. In one embodiment, the random stretch of oligonucleotides comprises about 8 oligonucleotides.

In one embodiment, the total number of synthetic templates in the first set of synthetic templates subject to amplification is used to determined using a limiting dilution of said synthetic templates each comprising a unique TCR of Ig V and J or C region such that the number of observed unique synthetic templates allows inference of the total number of input synthetic template molecules. In one embodiment, each synthetic template is found only in a single copy.

In one embodiment, the total number of synthetic templates in the first set of synthetic templates subject to amplification is determined by counting the number of unique synthetic templates based on unique random nucleotides contained in each synthetic template.

In one embodiment, the method provides for amplification of two or more genomic control regions. In another embodiment, the method provides for amplification of three or more genomic control regions. In yet another embodiment, the method provides for amplification of four or more genomic control regions. In still another embodiment, the method provides for amplification of five or more genomic control regions. In one embodiment, the method provides for amplification of five genomic control regions and calculating amplification factors for each. In one embodiment, the average amplification factor is determined by taking the average of amplification factors for each genomic control region. In one embodiment, the highest and lowest genomic control region amplification factor is discarded prior to taking an average. In one embodiment, the genomic control regions are one or more of ACTB, B2M, C1orf34, CHMP2A, GPI, GUSB, HMBS, HPRT1, PSMB4, RPL13A, RPLP0, SDHA, SNRPD#, UBC, VCP, VPS29, PPIA, PSMB2, RAB7A, UBC, VCP, REEP5 and EMC7. In one embodiment, the genomic control regions are PSMB2, RAB7A, PPIA, REEP5 and EMC7

In one embodiment, the multiplex PCR and sequencing of rearranged TCR or Ig loci and first synthetic templates are done in one multiplex PCR reaction while the amplification of the genomic control regions and second set of synthetic templates are done in a second multiplex PCR reaction. In another embodiment, the rearranged TCR and or Ig loci, the first set of synthetic templates, the genomic control regions and second set of synthetic templates are amplified and sequenced in the same multiplex PCR reaction.

In one embodiment, amplification of the rearranged TCR or Ig loci and first set of synthetic templates is done using a plurality of oligonucleotide primers. In one embodiment, the oligonucleotide primers comprise a plurality of V segment oligonucleotide primers that are each independently capable of specifically hybridizing to at least one polynucleotide encoding a TCR of Ig V region polypeptide or to the complement thereof. In one embodiment, each V segment primer comprises a nucleotide sequence of at least 15 contiguous nucleotides that is complementary to at least one functional a TCR or Ig V region encoding gene segment. In one embodiment, the plurality of V segment primers specifically hybridize to substantially all functional TCR or Ig V region encoding gene segments that are present in the composition. In one embodiment, the plurality of primers further includes a plurality of J segment oligonucleotide primers that are each independently capable of specifically hybridizing to at least one polynucleotide encoding a TCR or Ig J region polypeptide or to the complement thereof. In one embodiment, each J segment primer comprises a nucleotide sequence of at least 15 contiguous nucleotides that is complementary to at least one functional TCR or Ig J region encoding gene segment. In one embodiment, the plurality of J segment primers specifically hybridize to substantially all functional TCR or Ig J region encoding gene segments that are present in the composition.

In one embodiment, the plurality of V segment oligonucleotide primers and said plurality of J-segment oligonucleotide primers comprise the sequences set forth in SEQ ID NOs: 1-764. In one embodiment, the plurality of V segment oligonucleotide primers comprise sequences having at least 90% sequence identity to nucleotide sequences set forth in SEQ ID NOs: 1-120, 147-158, 167-276, 407-578, 593-740, and/or the plurality of J segment oligonucleotide primers comprise sequences having at least 90% sequence identity to nucleotide sequences set forth in SEQ ID NOs: 121-146, 159-166, 277-406, 579-592, or 741-764.

In one embodiment, the sample is fresh, frozen or fixed tissue. In one embodiment, the sample comprises human cells, mouse cells or rat cells. In one embodiment, the sample comprises somatic tissue.

In one embodiment, the sample is tumor biopsy. In one embodiment the TCR V segment comprises a TCR Vδ segment, a TCR Vγ segment, a TCR Vα segment, or a TCR Vβ segment. In one embodiment, the TCR J segment comprises a TCR Jδ segment, a TCR Jγ segment, a TCR Jα segment, or a TCR Jβ segment. In one embodiment, the Ig V segment comprises an IGH V gene segment, an IGL V gene segment, or an IGK V gene segment. In one embodiment, the Ig J region segment comprises an IGH J gene segment, an IGL J gene segment, or an IGK V gene segment.

In one embodiment, the biological output sequences for the TCR or Ig loci and the synthetic templates contained in the first set of synthetic templates are each about 100-300 nucleotides in length. In another embodiment, the output sequences for each genomic control region and the synthetic templates contained in the second set of synthetic templates are each about 100-300 nucleotides in length. In still another embodiment, the biological output sequences for the TCR or Ig loci, the synthetic templates contained in the first set of synthetic templates, the output sequences for each genomic control region and the synthetic templates contained in the second set of synthetic templates are each about 100-300 nucleotides in length.

In one embodiment, an amplification factor is determined for (i) a plurality of biological rearranged nucleic acid molecules encoding an adaptive immune receptor comprising a T-cell receptor (TCR) or Immunoglobulin (Ig) from said biological sample, each biological rearranged nucleic acid molecule comprising a unique variable (V) region encoding gene segment and a unique joining (J) region encoding gene segment, and (ii) a plurality of synthetic template oligonucleotide molecules, each comprising a paired combination of a unique V region gene segment and a unique J region gene segment found in one of the plurality of biological rearranged nucleic acid molecules.

In a further embodiment, determining the amplification factor includes comparing (1) a number of output synthetic template oligonucleotide sequences obtained from sequencing of amplified synthetic template oligonucleotide molecules generated from said multiplex PCR with (2) a number of input synthetic template oligonucleotide molecules added to said multiplex PCR, wherein said synthetic template oligonucleotide sequences comprise a sequence of formula I: 5′-U1-B1-V-B2-J-B3-U2-3′ (I).

Within the synthetic template oligonucleotide sequence of formula I: V is an oligonucleotide sequence comprising at least 20 and not more than 1000 contiguous nucleotides of an adaptive immune receptor variable (V) region encoding gene sequence, or the complement thereof, and each of said plurality of synthetic template oligonucleotide sequences having a unique V-region oligonucleotide sequence, J is an oligonucleotide sequence comprising at least 15 and not more than 600 contiguous nucleotides of an adaptive immune receptor joining (J) region encoding gene sequence, or the complement thereof, and each of said plurality of synthetic template oligonucleotide sequences having a unique J-region oligonucleotide sequence, U1 comprises an oligonucleotide sequence that is selected from (i) a first universal adaptor oligonucleotide sequence and (ii) a first sequencing platform-specific oligonucleotide sequence that is linked to and positioned 5′ to a first universal adaptor oligonucleotide sequence, U2 comprises an oligonucleotide sequence that is selected from (i) a second universal adaptor oligonucleotide sequence, and (ii) a second sequencing platform-specific oligonucleotide sequence that is linked to and positioned 5′ to a second universal adaptor oligonucleotide sequence, and at least one of B1, B2, and B3 is present and each of B1, B2, and B3 comprises an oligonucleotide comprising a barcode sequence of 3-25 contiguous nucleotides that uniquely identifies, as a paired combination, (i) said unique V-region oligonucleotide sequence of (a) and (ii) said unique J-region oligonucleotide sequence of (b).

In a further embodiment, a total number of input biological rearranged nucleic acid molecules is determined by comparing the number of output sequences of biological rearranged nucleic acid molecules obtained from sequencing of amplified biological rearranged nucleic acid molecules produced from said multiplex PCR with said amplification factor. In still further embodiment, the relative representation of adaptive immune cells (in a biological sample comprising a mixture of cells comprising adaptive immune cells and cells that are not adaptive immune cells) is determined by comparing said number of input biological rearranged nucleic acid molecules with said number of total input biological nucleic acid molecules.

In some embodiments, determining said amplification factor comprises dividing (1) said number of output synthetic template oligonucleotide sequences obtained from sequencing of amplified synthetic template oligonucleotide molecules generated from the multiplex PCR by (2) said number of input synthetic template oligonucleotides added to said multiplex PCR. In other embodiments, determining a number of input biological rearranged nucleic acid molecules comprises dividing (1) a total number of output sequences of biological rearranged nucleic acid molecules obtained from sequencing of amplified biological rearranged nucleic acid molecules produced from said multiplex PCR by (2) said amplification factor. In still other embodiments, comparing said number of input biological rearranged nucleic acid molecules with said number of total input biological nucleic acid molecules comprises dividing number of input biological rearranged nucleic acid molecules by said the number of total input biological nucleic acid molecules.

In an embodiment, said number of input synthetic template oligonucleotides added in said multiplex PCR is determined by amplifying an undiluted synthetic template oligonucleotide pool using simplex PCR to obtain a plurality of synthetic template amplicons, sequencing said plurality of synthetic template amplicons to determine a frequency of each unique synthetic template oligonucleotide in the pool, quantifying a relationship based on in silico simulations of said frequency of each unique synthetic template oligonucleotide in the pool, between a total number of unique observed synthetic template oligonucleotide sequences in a subset of the pool and the number of total synthetic template oligonucleotides present in said subset, and determining a number of input total synthetic template oligonucleotides in said multiplex PCR, said multiplex PCR including a limiting dilution of said synthetic template oligonucleotide pool, said determination based on the number of unique synthetic template oligonucleotides observed in the sequencing output of said simplex PCR and on said quantified relationship. In a further embodiment, said number of input synthetic template oligonucleotides added in said multiplex PCR is further determined by adding a known quantity of said pool of diluted synthetic template oligonucleotides to said multiplex PCR to produce a number of amplified total synthetic template oligonucleotides.

In an embodiment, said multiplex PCR is performed using a plurality of oligonucleotide primer sets comprising: (a) a plurality of V segment oligonucleotide primers that are each independently capable of specifically hybridizing to at least one polynucleotide encoding an adaptive immune receptor V region polypeptide or to the complement thereof, wherein each V segment primer comprises a nucleotide sequence of at least 15 contiguous nucleotides that is complementary to at least one functional adaptive immune receptor V region encoding gene segment and wherein said plurality of V segment primers specifically hybridize to substantially all functional adaptive immune receptor V region encoding gene segments that are present in the composition, and (b) a plurality of J segment oligonucleotide primers that are each independently capable of specifically hybridizing to at least one polynucleotide encoding an adaptive immune receptor J region polypeptide or to the complement thereof, wherein each J segment primer comprises a nucleotide sequence of at least 15 contiguous nucleotides that is complementary to at least one functional adaptive immune receptor J region encoding gene segment and wherein said plurality of J segment primers specifically hybridize to substantially all functional adaptive immune receptor J region encoding gene segments that are present in the composition, such that said plurality of V segment and J segment oligonucleotide primers are capable of amplifying in said multiplex PCR: (i) substantially all synthetic template oligonucleotides to produce a plurality of amplified synthetic template oligonucleotide molecules, and (ii) substantially all biological rearranged nucleic acid molecules encoding adaptive immune receptors in said biological sample to produce a plurality of amplified biological rearranged nucleic acid molecules, said plurality of amplified biological rearranged nucleic acid molecules being sufficient to quantify diversity of said rearranged nucleic acid molecules from said biological sample. In a further embodiment, said plurality of V segment oligonucleotide primers and said plurality of J-segment oligonucleotide primers comprise the sequences set forth in SEQ ID NOs: 1-764.

In another embodiment, either one of both of: (i) said plurality of V segment oligonucleotide primers comprise sequences having at least 90% sequence identity to nucleotide sequences set forth in SEQ ID NOs: 1-120, 147-158, 167-276, 407-578, or 593-740, and (ii) said plurality of J segment oligonucleotide primers comprise sequences having at least 90% sequence identity to nucleotide sequences set forth in SEQ ID NOs: 121-146, 159-166, 277-406, 579-592, or 741-764. In some embodiments, said plurality of synthetic template oligonucleotide molecules comprises a number of at least a or at least b unique oligonucleotide sequences, whichever is larger, wherein a is the number of unique adaptive immune receptor V region-encoding gene segments in the subject and b is the number of unique adaptive immune receptor J region-encoding gene segments in the subject. In a further embodiment, a ranges from 1 to a number of maximum V gene segments in the genome of said mammalian subject. In a further embodiment, b ranges from 1 to a number of maximum J gene segments in the genome of said mammalian subject. In other embodiments, said plurality of synthetic template oligonucleotide molecules comprises at least one synthetic template oligonucleotide sequence for each unique V region oligonucleotide sequence and at least one synthetic template oligonucleotide sequence for each unique J region oligonucleotide sequence. In some embodiments, said adaptive immune cells are T cells or B cells. In other embodiments, said biological sample is fresh tissue, frozen tissue, or fixed tissue, and said biological sample comprises human cells, mouse cells, or rat cells. In further embodiments, said biological sample comprises somatic tissue.

In an embodiment, said V region encoding gene segment comprises a TCR Vδ segment, a TCR Vγ segment, a TCR Vα segment, or a TCR Vβ segment. In another embodiment, said J region encoding gene segment comprises a TCR Jδ segment, a TCR Jγ segment, a TCR Jα segment, or a TCR Jβ segment. In some embodiments, said V region encoding gene segment comprises an IGH V gene segment, an IGL V gene segment, or an IGK V gene segment. In other embodiments, said J region encoding gene segment comprises an IGH J gene segment, an IGL J gene segment, or an IGK V gene segment. In some embodiments, said plurality of synthetic template oligonucleotide sequences comprise sequences selected from SEQ ID NOs: 787-3003. In other embodiments, V of formula (I) is an oligonucleotide sequence comprising at least 30, 60, 90, 120, 150, 180, or 210, or not more than 900, 800, 700, 600, or 500 contiguous nucleotides of an adaptive immune receptor V region encoding gene sequence, or the complement thereof. In other embodiments, J of formula (I) is an oligonucleotide sequence comprising at least 16-30, 31-60, 61-90, 91-120, or 120-150, or not more than 500, 400, 300, or 200 contiguous nucleotides of an adaptive immune receptor J region encoding gene sequence, or the complement thereof.

In some embodiments, J of formula (I) comprises a sequence comprising a constant region of J region encoding gene sequence. In other embodiments, each synthetic template oligonucleotide sequence is less than 1000, 900, 800, 700, 600, 500, 400, 300 or 200 nucleotides in length.

Also disclosed herein are kits comprising reagents comprising a composition comprising a plurality of synthetic template oligonucleotides and a set of oligonucleotide primers as described above, and instructions for quantifying a relative representation of adaptive immune cells in a biological sample that comprises a mixture of cells comprising adaptive immune cells and cells that are not adaptive immune cells, by quantifying: (i) a synthetic template product number of amplified synthetic template oligonucleotide molecules, and (ii) a biological rearranged product number of a number of output sequences.

BRIEF DESCRIPTIONS OF THE DRAWINGS

These and other features, aspects, and advantages of the present invention will become better understood with regard to the following description, and accompanying drawings, where:

FIGS. 1A and 1B illustrate examples of synthetic template oligonucleotides, according to one embodiment of the invention. Primers with Illumina (ILMN) sequencing tails are shown, specific for the universal adaptor sequences (above) or the V gene and J gene sequences (below).

FIG. 2 is a graph showing the relationship between the number of unique synthetic template oligonucleotides and the total number of synthetic template oligonucleotides in a sample, according to one embodiment of the invention.

FIG. 3 is a graph comparing a known number of B cell genomes in a sample with the estimated number of B cell genomes in the sample using synthetic template oligonucleotides, according to one embodiment of the invention.

FIG. 4 is a graph showing calculations of relative representations of T cells out of total cells in a sample, either via ddPCR methods or using synthetic template oligonucleotides, according to one embodiment of the invention.

FIG. 5 illustrates an example of a control synthetic template oligonucleotide and its relationship to a representative genomic locus, according to one embodiment of the invention, and the relationship of the control synthetic template oligonucleotide to the genomic locus it represents.

FIG. 6 illustrates that the methods of the current invention utilizing genomic control regions are able to accurately calculate the number of input genomes and number of T cells based on the number of input sequences.

DETAILED DESCRIPTION OF THE INVENTION

Overview

Methods and compositions are provided for determining the absolute number of input genomes from adaptive immune cells in a complex mixture of cells. In addition, the present disclosure relates to methods for quantitative determination of lymphocyte presence in complex tissues, such as solid tissues. The methods of the invention also include a quantification of the relative representation of tumor-infiltrating lymphocyte (TIL) genomes as a relative proportion of all cellular genomes that are represented in a sample, such as a solid tissue or solid tumor sample, or quantification of the genomes of lymphocytes that have infiltrated somatic tissue in the pathogenesis of inflammation, allergy or autoimmune disease or in transplanted organs as a relative proportion of all cellular genomes that are represented in a tissue DNA sample.

In some embodiments, the method includes determining the accurate absolute quantification of the number of adaptive immune cells in a biological sample. This involves determining the number of input genomes from adaptive immune cells in the sample.

Further provided herein are compositions and methods that are useful for reliably quantifying and determining the sequences of large and structurally diverse populations of rearranged genes encoding adaptive immune receptors, such as immunoglobulins (IG) and/or T cell receptors (TCR). These rearranged genes may be present in a biological sample containing DNA from lymphoid cells of a subject or biological source, including a human subject, and/or mRNA transcripts of these rearranged genes may be present in such a sample and used as templates for cDNA synthesis by reverse transcription.

Methods are provided for quantifying an amount of synthetic template oligonucleotides in a sample to determine a total number of input genomes from adaptive immune cells in a biological sample. In one embodiment, a sample of synthetic template oligonucleotides is used to determine a ratio of the number of input synthetic template oligonucleotide molecules compared with the number of total output (amplicon) synthetic template oligonucleotides. A limiting dilution of this sample is spiked-in to a biological sample (at the start of a multiplex PCR assay) and used to determine the total number of input genomes from adaptive immune cells in the biological sample. In certain embodiments, the synthetic templates in the sample comprise a stretch of random nucleic acids, for example an 8 nucleotide randomer. Therefore, limiting dilutions can be made such that each synthetic template in the sample is present only once and can be identified by the 8 nucleotide randomer contained therein.

The method also includes determining the relative representation of adaptive immune cells in a sample that contains a mixture of cells, where the mixture comprises adaptive immune cells and cells that are not adaptive immune cells. In certain embodiments, a relative representation of DNA from adaptive immune cells (e.g., T and/or B lymphocytes having rearranged adaptive immune receptor genes, including T- and B-lineage cells of different maturational stages such as precursors, blast cells, progeny or the like) among total DNA from a sample of mixed cell types can be quantified. For instance, certain embodiments permit determination, in DNA extracted from a biological sample, of the relative representation of DNA from tumor infiltrating lymphocytes (TIL) in the DNA from the biological sample, where the sample comprises all or a portion of a tumor that contains adaptive immune cells and cells that are not adaptive immune cells (including tumor cells). Certain other embodiments, for example, permit determination, in DNA extracted from a biological sample, of the relative representation of DNA from infiltrating lymphocytes in the DNA from the biological sample, where the sample comprises all or a portion of a somatic tissue that contains adaptive immune cells and cells that are not adaptive immune cells, such as cells of a solid tissue. Alternative methods of quantifying the relative representation of adaptive immune cells in a mixture of cells are disclosed in U.S. Ser. No. 13/656,265, filed on Oct. 21, 2012, and International App. No. PCT/US2012/061193, filed on Oct. 21, 2012, which are hereby incorporated by reference in their entireties.

The cells in the mixture of cells may not all be adaptive immune cells, and certain unforeseen advantages of the herein described embodiments are obtained where the cells in the mixture of cells need not all be adaptive immune cells. As described herein, compositions and methods are provided for quantifying the proportion of cellular genomes in a sample comprising nucleic acid molecules (e.g., DNA) that are contributed by adaptive immune cells relative to the total number of cellular genomes in the sample, starting from a DNA sample that has been extracted from a mixture of cell types, such as a solid tumor or a solid tissue.

In certain embodiments, rearranged adaptive immune receptor nucleic acid molecules are amplified in a single multiplex PCR using rearranged adaptive immune receptor-specific oligonucleotide primer sets to produce adaptive immune cell-specific DNA sequences, which are used to determine the relative contribution of adaptive immune cells as compared to the total DNA extracted from a sample of mixed cell types. In other embodiments, rearranged adaptive immune cell mRNA molecules are amplified using rt-qPCR and rearranged adaptive immune receptor-specific oligonucleotide primer sets to quantify rearranged adaptive immune receptor cDNA signals and to determine the relative contribution of adaptive immune cells to the total number of genomes extracted from a sample of mixed cell types. Methods of using qPCR to determine the relative representation of adaptive immune cells in a mixture of cells are disclosed in U.S. Ser. No. 13/656,265, filed on Oct. 21, 2012, and International App. No. PCT/US2012/061193, filed on Oct. 21, 2012, which are hereby incorporated by reference in their entireties.

Furthermore, in other embodiments, where the sample includes mRNA molecules, methods of the invention include using a real time quantitative polymerase chain reaction (qPCR) assay with oligonucleotide primer sets that specifically amplify substantially all rearranged adaptive immune receptor genes (e.g., CDR3 encoding polynucleotide-containing portions of rearranged T cell receptor and/or immunoglobulin genes) that may be present in a sample, to generate a first detectable DNA signal that quantitatively reflects the production of a multiplicity of amplified rearranged adaptive immune receptor encoding DNA molecules. In certain embodiments, qPCR amplification may be monitored at one or a plurality of time points during the course of the qPCR reaction, i.e., in “real time”. Real-time monitoring permits determination of the quantity of DNA that is being generated by comparing a so-measured adaptive immune receptor-encoding DNA-quantifying signal to an appropriate synthetic template (or control template DNA) quantifying signal, which may be used as a calibration standard. Methods for quantification using qPCR are described in detail in U.S. application Ser. No. 13/656,265, filed on Oct. 21, 2012, International App. No. PCT/US2012/061193, filed on Oct. 21, 2012, which are each incorporated by reference in their entireties.

Further disclosed herein are unexpectedly advantageous approaches for determining the relative representation of adaptive immune cells in a biological sample using multiplex PCR to generate a population of amplified DNA molecules from a biological sample containing rearranged genes encoding adaptive immune receptors, prior to quantitative high throughput sequencing of such amplified products. Multiplexed amplification and high throughput sequencing of rearranged TCR and BCR (IG) encoding DNA sequences are described, for example, in Robins et al., 2009 Blood 114, 4099; Robins et al., 2010 Sci. Translat. Med. 2:47ra64; Robins et al., 2011 J. Immunol. Meth. doi:10.1016/j.jim.2011.09.001; Sherwood et al. 2011 Sci. Translat. Med. 3:90ra61; U.S. Ser. No. 13/217,126 (US Pub. No. 2012/0058902), U.S. Ser. No. 12/794,507 (US Pub. No. 2010/0330571), WO/2010/151416, WO/2011/106738 (PCT/US2011/026373), WO2012/027503 (PCT/US2011/049012), U.S. Ser. No. 61/550,311, WO/2013/169957 (PCT/US2013/040221), WO/2013/188831 (PCT/US2013/045994), and U.S. Ser. No. 61/569,118; accordingly these disclosures are incorporated by reference and may be adapted for use according to the embodiments described herein.

Further described herein, in certain embodiments, are compositions and methods for the use of synthetic template oligonucleotides that are intended to be directly included in amplification and sequencing reactions of a sample, and whose quantity in the reaction (the number of molecules) can be precisely measured to improve the accuracy of multiplex PCR amplification bias correction and absolute input template quantitation. Amplification bias is described further in WO/2013/169957 (PCT/US2013/040221) and Carlson, C. S. et al. Using synthetic templates to design an unbiased multiplex PCR assay, Nature Communications 4, 2680, doi: 10.1038/ncomms3680 (2013), both of which are each incorporated by reference in its entirety.

The present invention is directed in certain embodiments as described herein to quantification of DNA from adaptive immune cells that are present in solid tissues, and in particular embodiments, to solid tumors, such that the relative presence of adaptive immune cells as a proportion of all cell types that may be present in the tissue (e.g., tumor) can be determined. These and related embodiments are in part a result of certain surprising and heretofore unrecognized advantages, disclosed in greater detail below, that derive from exquisite sensitivity that is afforded, for the detection of adaptive immune cells, by the design of multiplex PCR using the herein described oligonucleotide primer sets. These oligonucleotide primer sets permit production of amplified rearranged DNA molecules and synthetic template molecules that encode portions of adaptive immune receptors. These and related embodiments feature the selection of a plurality of oligonucleotide primers that specifically hybridize to adaptive immune receptor (e.g., T cell receptor, TCR; or immunoglobulin, Ig) V-region polypeptide encoding polynucleotide sequences and J-region polypeptide encoding polynucleotide sequences. The invention includes universal primers that are specific to universal adaptor sequences and bind to amplicons comprising universal adaptor sequences. The primers promote PCR amplification of nucleic acid molecules, such as DNA, that include substantially all rearranged TCR CDR3-encoding or Ig CDR3-encoding gene regions that may be present in a test biological sample, where the sample contains a mixture of cells which comprises adaptive immune cells (e.g., T- and B-lymphocyte lineage cells) and cells that are not adaptive immune cells. For example, a cell mixture may be obtained from a solid tumor that comprises tumor cells and TILs.

Adaptive Immune Cell Receptors

The native TCR is a heterodimeric cell surface protein of the immunoglobulin superfamily, which is associated with invariant proteins of the CD3 complex involved in mediating signal transduction. TCRs exist in αβ and γδ forms, which are structurally similar but have quite distinct anatomical locations and probably functions. The MHC class I and class II ligands, which bind to the TCR, are also immunoglobulin superfamily proteins but are specialized for antigen presentation, with a highly polymorphic peptide binding site which enables them to present a diverse array of short peptide fragments at the APC cell surface.

The extracellular portions of native heterodimeric αβ and γδ TCRs consist of two polypeptides each of which has a membrane-proximal constant domain, and a membrane-distal variable domain. Each of the constant and variable domains includes an intra-chain disulfide bond. The variable domains contain the highly polymorphic loops analogous to the complementarity determining regions (CDRs) of antibodies. CDR3 of αβ TCRs interact with the peptide presented by MHC, and CDRs 1 and 2 of αβ TCRs interact with the peptide and the MHC. The diversity of TCR sequences is generated via somatic rearrangement of linked variable (V), diversity (D), joining (J), and constant genes.

The Ig and TCR gene loci contain many different variable (V), diversity (D), and joining (J) gene segments, which are subjected to rearrangement processes during early lymphoid differentiation. Ig and TCR V, D and J gene segment sequences are known in the art and are available in public databases such as GENBANK. The V-D-J rearrangements are mediated via a recombinase enzyme complex in which the RAG1 and RAG2 proteins play a key role by recognizing and cutting the DNA at the recombination signal sequences (RSS). The RSS are located downstream of the V gene segments, at both sides of the D gene segments, and upstream of the J gene segments. Inappropriate RSS reduce or even completely prevent rearrangement. The RSS consists of two conserved sequences (heptamer, 5′-CACAGTG-3′, and nonamer, 5′-ACAAAAACC-3′), separated by a spacer of either 12+/−1 bp (“12-signal”) or 23+/−1 bp (“23-signal”). A number of nucleotide positions have been identified as important for recombination, including the CA dinucleotide at position one and two of the heptamer, and a C at heptamer position three has also been shown to be strongly preferred as well as an A nucleotide at positions 5, 6, 7 of the nonamer. (Ramsden et al. 1994 Nucl. Ac. Res. 22:1785; Akamatsu et al. 1994 J. Immunol. 153:4520; Hesse et al. 1989 Genes Dev. 3:1053). Mutations of other nucleotides have minimal or inconsistent effects. The spacer, although more variable, also has an impact on recombination, and single-nucleotide replacements have been shown to significantly impact recombination efficiency (Fanning et al. 1996 Cell. Immunol. Immumnopath. 79:1, Larijani et al. 1999 Nucl. Ac. Res. 27:2304; Nadel et al. 1998 J. Immunol. 161:6068; Nadel et al. 1998 J. Exp. Med. 187:1495). Criteria have been described for identifying RSS polynucleotide sequences having significantly different recombination efficiencies (Ramsden et al. 1994 Nucl. Ac. Res. 22:1785; Akamatsu et al. 1994 J. Immunol. 153:4520; Hesse et al. 1989 Genes Dev. 3:1053, and Lee et al., 2003 PLoS 1(1):E1).

The rearrangement process generally starts with a D to J rearrangement followed by a V to D-J rearrangement in the case of Ig heavy chain (IgH), TCR beta (TCRB), and TCR delta (TCRD) genes or concerns direct V to J rearrangements in case of Ig kappa (IgK), Ig lambda (IgL), TCR alpha (TCRA), and TCR gamma (TCRG) genes. The sequences between rearranging gene segments are generally deleted in the form of a circular excision product, also called TCR excision circle (TREC) or B cell receptor excision circle (BREC).

The many different combinations of V, D, and J gene segments represent the so-called combinatorial repertoire, which is estimated to be ˜2×106 for Ig molecules, ˜3×106 for TCRαβ and ˜5×103 for TCRγδ molecules. At the junction sites of the V, D, and J gene segments, deletion and random insertion of nucleotides occurs during the rearrangement process, resulting in highly diverse junctional regions, which significantly contribute to the total repertoire of Ig and TCR molecules, estimated to be >1012.

Mature B-lymphocytes further extend their Ig repertoire upon antigen recognition in follicle centers via somatic hypermutation, a process, leading to affinity maturation of the Ig molecules. The somatic hypermutation process focuses on the V- (D-) J exon of IgH and Ig light chain genes and concerns single nucleotide mutations and sometimes also insertions or deletions of nucleotides. Somatically-mutated Ig genes are also found in mature B-cell malignancies of follicular or post-follicular origin.

Definitions

As used herein, the term “gene” refers to a segment of DNA that can be expressed as a polypeptide chain. The polypeptide chain can be all or a portion of a TCR or Ig polypeptide (e.g., a CDR3-containing polypeptide). The gene can include regions preceding and following the coding region (“leader and trailer”), intervening sequences (introns) between individual coding segments (exons), regulatory elements (e.g., promoters, enhancers, repressor binding sites and the like), and recombination signal sequences (RSS's), as described herein.

The “nucleic acids” or “nucleic acid molecules” or “polynucleotides” or “oligonucleotides” can be in the form of ribonucleic acids (RNA), or in the form of deoxyribonucleic acids (DNA). As referred to herein, RNA includes mRNA. DNA includes cDNA, genomic DNA, and synthetic DNA. The DNA can be double-stranded or single-stranded, and if single stranded may be the coding strand or non-coding (anti-sense) strand. A coding sequence which encodes a TCR or an immunoglobulin or a region thereof (e.g., a V region, a D segment, a J region, a C region, etc.) can be identical to the coding sequence known in the art for any given TCR or immunoglobulin gene regions or polypeptide domains (e.g., V-region domains, CDR3 domains, etc.). In other embodiments, the coding sequence can be a different coding sequence, which, as a result of the redundancy or degeneracy of the genetic code, encodes the same TCR or immunoglobulin region or polypeptide.

The term “primer,” as used herein, refers to an oligonucleotide capable of acting as a point of initiation of DNA synthesis under suitable conditions. Such conditions include those in which synthesis of a primer extension product complementary to a nucleic acid strand is induced in the presence of four different nucleoside triphosphates and an agent for extension (e.g., a DNA polymerase or reverse transcriptase) in an appropriate buffer and at a suitable temperature.

A primer is preferably a single-stranded DNA. The appropriate length of a primer depends on the intended use of the primer but typically ranges from 6 to 50 nucleotides, or in certain embodiments, from 15-35 nucleotides. Short primer molecules generally require cooler temperatures to form sufficiently stable hybrid complexes with the template. A primer need not reflect the exact sequence of the template nucleic acid, but must be sufficiently complementary to hybridize with the template. The design of suitable primers for the amplification of a given target sequence is well known in the art and described in the literature cited herein.

As described herein, primers can incorporate additional features which allow for the detection or immobilization of the primer but do not alter the basic property of the primer, that of acting as a point of initiation of DNA synthesis. For example, primers may contain an additional nucleic acid sequence at the 5′ end which does not hybridize to the target nucleic acid, but which facilitates cloning, detection, or sequencing of the amplified product. The region of the primer which is sufficiently complementary to the template to hybridize is referred to herein as the hybridizing region.

As used herein, a primer is “specific,” for a target sequence if, when used in an amplification reaction under sufficiently stringent conditions, the primer hybridizes primarily to the target nucleic acid. Typically, a primer is specific for a target sequence if the primer-target duplex stability is greater than the stability of a duplex formed between the primer and any other sequence found in the sample. One of skill in the art will recognize that various factors, such as salt conditions as well as base composition of the primer and the location of the mismatches, will affect the specificity of the primer, and that routine experimental confirmation of the primer specificity will be needed in many cases. Hybridization conditions can be chosen under which the primer can form stable duplexes only with a target sequence. Thus, the use of target-specific primers under suitably stringent amplification conditions enables the selective amplification of those target sequences which contain the target primer binding sites.

The term “ameliorating” refers to any therapeutically beneficial result in the treatment of a disease state, e.g., a cancer stage, an autoimmune disease state, including prophylaxis, lessening in the severity or progression, remission, or cure thereof.

The term “in vivo” refers to processes that occur in a living organism.

The term “mammal” as used herein includes both humans and non-humans and include but is not limited to humans, non-human primates, canines, felines, murines, bovines, equines, and porcines.

The term percent “identity,” in the context of two or more nucleic acid or polypeptide sequences, refer to two or more sequences or subsequences that have a specified percentage of nucleotides or amino acid residues that are the same, when compared and aligned for maximum correspondence, as measured using one of the sequence comparison algorithms described below (e.g., BLASTP and BLASTN or other algorithms available to persons of skill) or by visual inspection. Depending on the application, the percent “identity” can exist over a region of the sequence being compared, e.g., over a functional domain, or, alternatively, exist over the full length of the two sequences to be compared.

For sequence comparison, typically one sequence acts as a reference sequence to which test sequences are compared. When using a sequence comparison algorithm, test and reference sequences are input into a computer, subsequence coordinates are designated, if necessary, and sequence algorithm program parameters are designated. The sequence comparison algorithm then calculates the percent sequence identity for the test sequence(s) relative to the reference sequence, based on the designated program parameters.

Optimal alignment of sequences for comparison can be conducted, e.g., by the local homology algorithm of Smith & Waterman, Adv. Appl. Math. 2:482 (1981), by the homology alignment algorithm of Needleman & Wunsch, J. Mol. Biol. 48:443 (1970), by the search for similarity method of Pearson & Lipman, Proc. Nat'l. Acad. Sci. USA 85:2444 (1988), by computerized implementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group, 575 Science Dr., Madison, Wis.), or by visual inspection (see generally Ausubel et al., infra).

One example of an algorithm that is suitable for determining percent sequence identity and sequence similarity is the BLAST algorithm, which is described in Altschul et al., J. Mol. Biol. 215:403-410 (1990). Software for performing BLAST analyses is publicly available through the National Center for Biotechnology Information (www.ncbi.nlm.nih.gov/).

It must be noted that, as used in the specification and the appended claims, the singular forms “a,”“an” and “the” include plural referents unless the context clearly dictates otherwise.

Samples (Tissues and Use)

As used herein, a sample, test sample or test biological sample refer to biological tissues (e.g., an aggregate of cells that have similar structure and function) obtained from a subject of interest. The sample can include a complex mixture of adaptive immune cells (e.g., T- and B-lymphocyte lineage cells) and cells that are not adaptive immune cells (e.g., solid tumor cells).

In certain embodiments, a test biological sample of interest comprises somatic tissue. The somatic tissue can comprise a solid tissue. In some embodiments, the solid tissue can be a site for autoimmune disease pathology, such as a tissue that is inappropriately targeted by a host's immune system for an “anti-self” immune response. In certain other embodiments, the somatic tissue can comprise a solid tissue that is a site of an infection, such as a bacterial, yeast, viral or other microbial infection (e.g., a Herpes Simplex Virus (HSV) infection). In yet other embodiments, the somatic tissue is obtained from a transplanted organ (e.g., a transplanted liver, lung, kidney, heart, spleen, pancreas, skin, intestine and thymus).

Samples can be obtained from tissues prior to, during, and/or post treatment. Samples can be used in diagnostic, prognostic, disease monitoring, therapeutic efficacy monitoring and other contexts, thereby providing important information, such as quantification of adaptive immune cell representation in complex tissues comprising a mixture of cells. Adaptive immune cell quantification (e.g., quantification of the relative representation of adaptive immune cells in samples) or adaptive immune cell DNA quantification (e.g., quantification of the relative representation of adaptive immune cell DNA in samples that contain DNA from a mixture of cells) in tissues before and after, and/or during the course of treatment of a subject, can provide information of relevance to the diagnosis and prognosis in patients with cancer, inflammation and/or autoimmune disease, or any of a number of other conditions that may be characterized by alterations (e.g., statistically significant increases or decreases) in adaptive immune cell presence in one or more tissues.

In some embodiments, the sample is obtained from a solid tumor in a subject. Multiple samples can be obtained prior to, during and/or following administration of a therapeutic regimen to the subject. A sample can be obtained, for example, by excision of tissue from a pre- or post-treatment subject.

In other embodiments, the sample comprising tissue is evaluated or analyzed according to other art-accepted criteria. Indicators of status (e.g., evidence of presence or absence of pathology, or of efficacy of a previously or contemporaneously administered therapeutic treatment) can be, for example, detectable indicator compounds, nanoparticles, nanostructures or other compositions that comprise a reporter molecule which provides a detectable signal indicating the physiological status of a cell or tissue, such as a vital dye (e.g., Trypan blue), a colorimetric pH indicator, a fluorescent compound that may exhibit distinct fluorescence as a function of any of a number of cellular physiological parameters (e.g., pH, intracellular Ca2+ or other physiologically relevant ion concentration, mitochondrial membrane potential, plasma membrane potential, etc., see Haugland, The Handbook: A Guide to Fluorescent Probes and Labeling Technologies (10th Ed.) 2005, Invitrogen Corp., Carlsbad, Calif.), an enzyme substrate, a specific oligonucleotide probe, a reporter gene, or the like.

Subjects and Source

The subject or biological source, from which a test biological sample may be obtained, may be a human or non-human animal, or a transgenic or cloned or tissue-engineered (including through the use of stem cells) organism. In certain preferred embodiments of the invention, the subject or biological source may be known to have, or may be suspected of having or being at risk for having, a solid tumor or other malignant condition, or an autoimmune disease, or an inflammatory condition, and in certain preferred embodiments of the invention the subject or biological source may be known to be free of a risk or presence of such disease.

Certain preferred embodiments contemplate a subject or biological source that is a human subject such as a patient that has been diagnosed as having or being at risk for developing or acquiring cancer according to art-accepted clinical diagnostic criteria, such as those of the U.S. National Cancer Institute (Bethesda, Md., USA) or as described in DeVita, Hellman, and Rosenberg's Cancer: Principles and Practice of Oncology (2008, Lippincott, Williams and Wilkins, Philadelphia/Ovid, N.Y.); Pizzo and Poplack, Principles and Practice of Pediatric Oncology (Fourth edition, 2001, Lippincott, Williams and Wilkins, Philadelphia/Ovid, N.Y.); and Vogelstein and Kinzler, The Genetic Basis of Human Cancer (Second edition, 2002, McGraw Hill Professional, New York); certain embodiments contemplate a human subject that is known to be free of a risk for having, developing or acquiring cancer by such criteria.

Certain embodiments contemplate a non-human subject or biological source, including, but not limited to, a non-human primate, such as a macaque, chimpanzee, gorilla, vervet, orangutan, baboon, or other non-human primate, including such non-human subjects that may be known to the art as preclinical models, including preclinical models for solid tumors and/or other cancers. Certain other embodiments contemplate a non-human subject that is a mammal, for example, a mouse, rat, rabbit, pig, sheep, horse, bovine, goat, gerbil, hamster, guinea pig or other mammal. Many such mammals may be subjects that are known to the art as preclinical models for certain diseases or disorders, including solid tumors and/or other cancers (e.g., Talmadge et al., 2007 Am. J. Pathol. 170:793; Kerbel, 2003 Canc. Biol. Therap. 2(4 Suppl 1):S134; Man et al., 2007 Canc. Met. Rev. 26:737; Cespedes et al., 2006 Clin. Transl. Oncol. 8:318). The range of embodiments is not intended to be so limited, however, such that there are also contemplated other embodiments in which the subject or biological source can be a non-mammalian vertebrate, for example, another higher vertebrate, or an avian, amphibian or reptilian species, or another subject or biological source.

Biological samples can be provided by obtaining a blood sample, biopsy specimen, tissue explant, organ culture, biological fluid or any other tissue or cell preparation from a subject or a biological source. In certain preferred embodiments, a test biological sample can be obtained from a solid tissue (e.g., a solid tumor), for example by surgical resection, needle biopsy or other means for obtaining a test biological sample that contains a mixture of cells.

Solid tissues are well known to the medical arts and can include any cohesive, spatially discrete non-fluid defined anatomic compartment that is substantially the product of multicellular, intercellular, tissue and/or organ architecture, such as a three-dimensionally defined compartment that may comprise or derive its structural integrity from associated connective tissue and may be separated from other body areas by a thin membrane (e.g., meningeal membrane, pericardial membrane, pleural membrane, mucosal membrane, basement membrane, omentum, organ-encapsulating membrane, or the like). Non-limiting exemplary solid tissues can include brain, liver, lung, kidney, prostate, ovary, spleen, lymph node (including tonsil), skin, thyroid, pancreas, heart, skeletal muscle, intestine, larynx, esophagus and stomach. Anatomical locations, morphological properties, histological characterization, and invasive and/or non-invasive access to these and other solid tissues are all well known to those familiar with the relevant arts.

Solid tumors of any type are contemplated as being suitable for characterization of TIL using the compositions and methods described herein. In certain preferred embodiments, the solid tumor can be a benign tumor or a malignant tumor, which can further be a primary tumor, an invasive tumor or a metastatic tumor. Certain embodiments contemplate a solid tumor that comprises one of a prostate cancer cell, a breast cancer cell, a colorectal cancer cell, a lung cancer cell, a brain cancer cell, a renal cancer cell, a skin cancer cell (such as squamous cell carcinoma, basal cell carcinoma, or melanoma) and an ovarian cancer cell, but the invention is not intended to be so limited and other solid tumor types and cancer cell types may be used. For example, the tumor may comprise a cancer selected from adenoma, adenocarcinoma, squamous cell carcinoma, basal cell carcinoma, melanoma (e.g., malignant melanoma), small cell carcinoma, large cell undifferentiated carcinoma, chondrosarcoma and fibrosarcoma, or the like. As also noted elsewhere herein, art-accepted clinical diagnostic criteria have been established for these and other cancer types, such as those promulgated by the U.S. National Cancer Institute (Bethesda, Md., USA) or as described in DeVita, Hellman, and Rosenberg's Cancer: Principles and Practice of Oncology (2008, Lippincott, Williams and Wilkins, Philadelphia/Ovid, N.Y.); Pizzo and Poplack, Principles and Practice of Pediatric Oncology (Fourth edition, 2001, Lippincott, Williams and Wilkins, Philadelphia/Ovid, N.Y.); and Vogelstein and Kinzler, The Genetic Basis of Human Cancer (Second edition, 2002, McGraw Hill Professional, New York). Other non-limiting examples of typing and characterization of particular cancers are described, e.g., in Ignatiadis et al. (2008 Pathobiol. 75:104); Kunz (2008 Curr. Drug Discov. Technol. 5:9); and Auman et al. (2008 Drug Metab. Rev. 40:303).

B cells and T cells can be obtained from a biological sample, such as from a variety of tissue and biological fluid samples including bone marrow, thymus, lymph glands, lymph nodes, peripheral tissues and blood, but peripheral blood is most easily accessed. Any peripheral tissue can be sampled for the presence of B and T cells and is therefore contemplated for use in the methods described herein. Tissues and biological fluids from which adaptive immune cells can be obtained include, but are not limited to skin, epithelial tissues, colon, spleen, a mucosal secretion, oral mucosa, intestinal mucosa, vaginal mucosa or a vaginal secretion, cervical tissue, ganglia, saliva, cerebrospinal fluid (CSF), bone marrow, cord blood, serum, serosal fluid, plasma, lymph, urine, ascites fluid, pleural fluid, pericardial fluid, peritoneal fluid, abdominal fluid, culture medium, conditioned culture medium or lavage fluid. In certain embodiments, adaptive immune cells can be isolated from an apheresis sample. Peripheral blood samples may be obtained by phlebotomy from subjects. Peripheral blood mononuclear cells (PBMCs) are isolated by techniques known to those of skill in the art, e.g., by Ficoll-Hypaque® density gradient separation. In certain embodiments, whole PBMCs are used for analysis.

In certain related embodiments, samples that comprise predominantly lymphocytes (e.g., T and B cells) or that comprise predominantly T cells or predominantly B cells, can be prepared for use as provided herein, according to established, art-accepted methodologies.

In other related embodiments, specific subpopulations of T or B cells can be isolated prior to analysis, using the methods described herein. Various methods and commercially available kits for isolating different subpopulations of T and B cells are known in the art and include, but are not limited to, subset selection immunomagnetic bead separation or flow immunocytometric cell sorting using antibodies specific for one or more of any of a variety of known T and B cell surface markers. Illustrative markers include, but are not limited to, one or a combination of CD2, CD3, CD4, CD8, CD14, CD19, CD20, CD25, CD28, CD45RO, CD45RA, CD54, CD62, CD62L, CDw137 (41BB), CD154, GITR, FoxP3, CD54, and CD28. For example, as known to a skilled person in the art, cell surface markers, such as CD2, CD3, CD4, CD8, CD14, CD19, CD20, CD45RA, and CD45RO can be used to determine T, B, and monocyte lineages and subpopulations using flow cytometry. Similarly, forward light-scatter, side-scatter, and/or cell surface markers, such as CD25, CD62L, CD54, CD137, and CD154, can be used to determine activation state and functional properties of cells.

Illustrative combinations useful in certain of the methods described herein can include CD8+CD45RO+ (memory cytotoxic T cells), CD4+CD45RO+ (memory T helper), CD8+CD45RO (CD8+CD62L+CD45RA+ (naïve-like cytotoxic T cells); CD4+CD25+CD62LhiGITR+FoxP3+ (regulatory T cells). Illustrative antibodies for use in immunomagnetic cell separations or flow immunocytometric cell sorting include fluorescently labeled anti-human antibodies, e.g., CD4 FITC (clone M-T466, Miltenyi Biotec), CD8 PE (clone RPA-T8, BD Biosciences), CD45RO ECD (clone UCHL-1, Beckman Coulter), and CD45RO APC (clone UCHL-1, BD Biosciences). Staining of cells can be done with the appropriate combination of antibodies, followed by washing cells before analysis. Lymphocyte subsets can be isolated by fluorescence activated cell sorting (FACS), e.g., by a BD FACSAria™ cell-sorting system (BD Biosciences) and by analyzing results with FlowJo™ software (Treestar Inc.), and also by conceptually similar methods involving specific antibodies immobilized to surfaces or beads.

For nucleic acid extraction, total genomic DNA can be extracted from cells using methods known in the art and/or commercially available kits, e.g., by using the QIAamp® DNA blood Mini Kit (QIAGEN®). The approximate mass of a single haploid genome is 3 picograms (pg). In some embodiments, a single diploid genome is approximately 6.5 picograms. In an embodiment, the absolute number of T cells can be estimated by assuming one total cell of input material per 6.5 picograms of genomic data. In some embodiments, at least 100,000 to 200,000 cells are used for analysis, i.e., about 0.6 to 1.2 μg DNA from diploid T or B cells.

Multiplex PCR

As described herein, there is provided a method for quantifying the relative representation of adaptive immune cell DNA in DNA from a test biological sample of mixed cell types, and thus for estimating the relative number of T or B cells in a complex mixture of cells. According to certain embodiments, the method for quantifying the relative representation of adaptive immune cell DNA in a complex mixture of cells involves a multiplex PCR method using a set of forward primers that specifically hybridize to the V segments and a set of reverse primers that specifically hybridize to the J segments, where the multiplex PCR reaction allows amplification of all the possible VJ (and VDJ) combinations within a given population of T or B cells. In some embodiments, the multiplex PCR method includes using the set of forward V-segment primers and set of reverse J-segment primers to amplify a given population of synthetic template oligonucleotides comprising the VJ and VDJ combinations. Because the multiplex PCR reaction amplifies substantially all possible combinations of V and J segments, it is possible to determine, using multiplex PCR, the relative number of T cell or B cell genomes in a sample comprising a mixed population of cells.

Nucleic Acid Extraction

In one embodiment, total genomic DNA can be extracted from cells using standard methods known in the art and/or commercially available kits, e.g., by using the QIAamp® DNA blood Mini Kit (QIAGEN®). The approximate mass of a single haploid genome is 3 pg. Preferably, at least 100,000 to 200,000 cells are used for analysis of diversity, i.e., about 0.6 to 1.2 μg DNA from diploid T or B cells.

Alternatively, total nucleic acid can be isolated from cells, including both genomic DNA and mRNA. If diversity is to be measured from mRNA in the nucleic acid extract, the mRNA must be converted to cDNA prior to measurement. This can readily be done by methods of one of ordinary skill, for example, using reverse transcriptase according to known procedures.

In some embodiments, DNA or mRNA can be extracted from a sample comprising a mixed population of cells. In certain embodiments, the sample can be a neoplastic tissue sample or somatic tissue. Illustrative samples for use in the present methods include any type of solid tumor, in particular, a solid tumor from colorectal, hepatocellular, gallbladder, pancreatic, esophageal, lung, breast, prostate, head and neck, renal cell carcinoma, ovarian, endometrial, cervical, bladder and urothelial cancers. Any solid tumor in which tumor-infiltrating lymphocytes are to be assessed is contemplated for use in the present methods. Somatic tissues that are the target of an autoimmune reaction include, but are not limited to, joint tissues, skin, intestinal tissue, all layers of the uvea, iris, vitreous tissue, heart, brain, lungs, blood vessels, liver, kidney, nerve tissue, muscle, spinal cord, pancreas, adrenal gland, tendon, mucus membrane, lymph node, thyroid, endometrium, connective tissue, and bone marrow. In certain embodiments, DNA or RNA can be extracted from a transplanted organ, such as a transplanted liver, lung, kidney, heart, spleen, pancreas, skin, intestine, and thymus.

In other embodiments, two or more samples can be obtained from a single tissue (e.g., a single neoplastic tissue) and the relative representations of adaptive immune cells in the two or more samples are quantified to consider variations in different sections of a test tissue. In certain other embodiments, the determination of the relative representation of adaptive immune cells in one sample from a test tissue is sufficient due to minimum variations among different sections of the test tissue.

Compositions (Primers for Multiplex PCR)

Compositions are provided for use in a multiplex PCR that comprise a plurality of V-segment primers and a plurality of J-segment primers that are capable of promoting amplification of substantially all productively rearranged adaptive immune receptor CDR3-encoding regions in a sample to produce a multiplicity of amplified rearranged DNA molecules from a population of T cells (for TCR) or B cells (for Ig) in the sample.

The TCR and Ig genes can generate millions of distinct proteins via somatic mutation. Because of this diversity-generating mechanism, the hypervariable complementarity determining regions of these genes can encode sequences that can interact with millions of ligands, and these regions are linked to a constant region that can transmit a signal to the cell indicating binding of the protein's cognate ligand. The adaptive immune system employs several strategies to generate a repertoire of T- and B-cell antigen receptors with sufficient diversity to recognize the universe of potential pathogens. In αβ and γδ T cells, which primarily recognize peptide antigens presented by MHC molecules, most of this receptor diversity is contained within the third complementarity-determining region (CDR3) of the T cell receptor (TCR) α and β chains (or γ and δ chains).

In the human genome, there are currently believed to be about 70 TCR Vα and about 61 Jα gene segments, about 52 TCR Vβ, about 2 DP and about 13 Jβ gene segments, about 9 TCR Vγ and about 5 Jγ gene segments, and about 46 immunoglobulin heavy chain (IGH) VH, about 23 DH and about 6 JH gene segments. Accordingly, where genomic sequences for these loci are known such that specific molecular probes for each of them can be readily produced, it is believed, according to non-limiting theory, that the present compositions and methods relate to substantially all (e.g., greater than 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99%) of these known and readily detectable adaptive immune receptor V-, D- and J-region encoding gene segments.

In one embodiment, the compositions of the invention provide a plurality of V-segment primers and a plurality of J-segment primers that are capable of amplifying substantially all combinations of the V and J segments of a rearranged immune receptor locus. The term “substantially all combinations” refers to at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more of all the combinations of the V- and J-segments of a rearranged immune receptor locus. In certain embodiments, the plurality of V-segment primers and the plurality of J-segment primers amplify all of the combinations of the V and J segments of a rearranged immune receptor locus. In certain embodiments, the plurality of V-segment and J-segment primers can each comprise or consist of a nucleic acid sequence that is the same as, complementary to, or substantially complementary to a contiguous sequence of a target V- or J-region encoding segment (i.e., portion of genomic polynucleotide encoding a V-region or J-region polypeptide, or a portion of mRNA).

In some embodiments, the V-segment and J-segment primers are “fully complementary” to a contiguous sequence of a target V- or J-region encoding segment, respectively. In other embodiments, the V-segment and J-segment primers are “substantially complementary” with respect to contiguous sequence of a target V- or J-region encoding segment. Generally there are no more than 4, 3 or 2 mismatched base pairs upon hybridization, while retaining the ability to hybridize under the conditions most relevant to their ultimate application.

In certain embodiments, two pools of primers are designed for use in a highly multiplexed PCR reaction. The first “forward” pool can include oligonucleotide primers that are each specific to (e.g., having a nucleotide sequence complementary to a unique sequence region of) each V-region encoding segment (“V segment”) in the respective TCR or Ig gene locus. In certain embodiments, primers targeting a highly conserved region are used, to simultaneously capture many V segments, thereby reducing the number of primers required in the multiplex PCR. In this manner, a V-segment primer can be complementary to (e.g., hybridize to) more than one functional TCR or Ig V-region encoding segment and act as a promiscuous primer. In other embodiments, each V-segment primer is specific for a different, functional TCR or Ig V-region encoding segment.

The “reverse” pool primers can include oligonucleotide primers that are each specific to (e.g., having a nucleotide sequence complementary to a unique sequence region of) each J-region encoding segment (“J segment”) in the respective TCR or Ig gene locus. In some embodiments, the J-primer can anneal to a conserved sequence in the joining (“J”) segment. In certain embodiments, a J-segment primer can be complementary to (e.g., hybridize to) more than one J-segment. In other embodiments, each J-segment primer is specific to a different, functional TCR or Ig J-region encoding segment. By way of illustration and not limitation, V-segment primers can be used as “forward” primers and J-segment primers can be used as “reverse” primers, according to commonly used PCR terminology, but the skilled person will appreciate that in certain other embodiments J-segment primers may be regarded as “forward” primers when used with V-segment “reverse” primers.

In some embodiments, the V-segment or J-segment primer is at least 15 nucleotides in length. In other embodiments, the V-segment or J-segment primer is at least 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 45, or 50 nucleotides in length and has the same sequence as, or is complementary to, a contiguous sequence of the target V- or J-region encoding segment. In some embodiments, the length of the primers may be longer, such as about 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 80, 85, 90, 95, 100 or more nucleotides in length or more, depending on the specific use or need. All intermediate lengths of the presently described primers are contemplated for use herein. As would be recognized by the skilled person, the primers can comprise additional sequences (e.g., nucleotides that may not be the same as or complementary to the target V- or J-region encoding polynucleotide segment), such as restriction enzyme recognition sites, universal adaptor sequences for sequencing, bar code sequences, chemical modifications, and the like (see e.g., primer sequences provided in the sequence listing herein).

In other embodiments, the V-segment or J-segment primers comprise sequences that share a high degree of sequence identity to the oligonucleotide primers for which nucleotide sequences are presented herein, including those set forth in the Sequence Listing. In certain embodiments, the V-segment or J-segment primers comprise primer variants that may have substantial identity to the adaptive immune receptor V-segment or J-segment primer sequences disclosed herein. For example, such oligonucleotide primer variants may comprise at least 70% sequence identity, preferably at least 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% or higher sequence identity compared to a reference oligonucleotide sequence, such as the oligonucleotide primer sequences disclosed herein, using the methods described herein (e.g., BLAST analysis using standard parameters). One skilled in this art will recognize that these values can be appropriately adjusted to determine corresponding ability of an oligonucleotide primer variant to anneal to an adaptive immune receptor segment-encoding polynucleotide by taking into account codon degeneracy, reading frame positioning and the like. Typically, oligonucleotide primer variants will contain one or more substitutions, additions, deletions and/or insertions, preferably such that the annealing ability of the variant oligonucleotide is not substantially diminished relative to that of an adaptive immune receptor V-segment or J-segment primer sequence that is specifically set forth herein. In other embodiments, the V-segment or J-segment primers are designed to be capable of amplifying a rearranged TCR or IGH sequence that includes the coding region for CDR3.

In some embodiments, as described herein, the plurality of V-segment and J-segment primers each comprise additional sequences at the 5′ end, such as universal adaptor sequences, bar code sequences, random oligonucleotide sequences, and the like. The sequences can be non-naturally occurring sequences and/or sequences that do not naturally appear adjacent to contiguous with a target V- or J-region encoding segment.

In certain embodiments, the plurality of V-segment and J-segment primers are designed to produce amplified rearranged DNA molecules that are less than 600 nucleotides in length, thereby excluding amplification products from non-rearranged adaptive immune receptor loci. In some embodiments, the amplified rearranged DNA molecules are at least 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, or 600 nucleotides in length. In one embodiment, the amplified rearranged DNA molecule is at least 250 nucleotides in length. In another embodiment, the amplified rearranged DNA molecule is approximately 200 nucleotides in length. The amplified rearranged DNA molecule can be referred to as an amplicon, amplified molecule, PCR product, or amplification product, for example.

An exemplary multiplex PCR assay uses a plurality of forward V-segment primers and a plurality of reverse J-segment primers to selectively amplify the rearranged VDJ from each cell. While these primers can anneal to both rearranged and germline V and J gene segments, PCR amplification is limited to rearranged gene segments, due to size bias (e.g., 250 bp PCR product using rearranged gene segments as templates vs. >10 Kb PCR product using germline gene segments as templates).

In some embodiments, primer selection and primer set design can be performed in a manner that preferably detects productive V and J gene segments, and excludes TCR or IG pseudogenes. Pseudogenes may include V segments that contain an in-frame stop codon within the V-segment coding sequence, a frameshift between the start codon and the CDR3 encoding sequence, one or more repeat-element insertions, and deletions of critical regions, such as the first exon or the RSS. In the human IGH locus, for instance, the ImmunoGeneTics (IMGT) database (M.-P. LeFranc, Université Montpellier, Montpellier, France; www.imgt.org) annotates 165 V segment genes, of which 26 are orphons on other chromosomes and 139 are in the IGH locus at chromosome 14. Among the 139 V segments within the IGH locus, 51 have at least one functional allele, while 6 are ORFs (open-reading frames) which are missing at least one highly conserved amino-acid residue, and 81 are pseudogenes.

To detect functional TCR or IG rearrangements in a sample while avoiding potentially extraneous amplification signals that may be attributable to non-productive V and/or J gene segments such as pseudogenes and/or orphons, it is therefore contemplated according to certain embodiments to use a subset of oligonucleotide primers which are designed to include only those V segments that participate in a functional rearrangement to encode a TCR or IG, without having to include amplification primers specific to the pseudogene and/or orphon sequences or the like. Advantageous efficiencies with respect, inter alia, to time and expense are thus obtained.

The plurality of V-segment primers and J-segment primers are designed to sit outside regions where untemplated deletions occur. These V-segment primer and J-segment primer positions are relative to the V gene recombination signal sequence (V-RSS) and J gene recombination signal sequence (J-RSS) in the gene segment. In some embodiments, the V-segment primers and J-segment primers are designed to provide adequate sequence information in the amplified product to identify both the V and J genes uniquely.

In some embodiments, each of the V-segment primers comprises a first sequence and a second sequence, wherein the first sequence is located 3′ to the second sequence on the V-segment primer. In certain embodiments, the first sequence is complementary to a portion of a first region of at least one V-segment, and the first region of the V-segment is located immediately 5′ to a second region of the V-segment where untemplated deletions occur during TCR or IG gene rearrangement. The second region of the V-segment is adjacent to and 5′ to a V-recombination signal sequence (V-RSS) of the V-segment. The second region where untemplated deletions occur on the V-segment can be at least 10 base pairs (bps) in length. In one embodiment, the 3′-end of the V-segment primer can be placed at least 10 bps upstream from the V-RSS. In some embodiments, the V-segment primer is placed greater than 40 base pairs of sequence upstream of the V-RSS.

In other embodiments, each of the J-segment primers has a first sequence and a second sequence, wherein the first sequence is located 3′ to the second sequence on the J-segment primer. The first sequence of the J-segment primer is complementary to a portion of a first region of a J-segment, and the first region of the J-segment is located immediately 3′ to a second region of the J-segment where untemplated deletions occur during TCR or IG gene rearrangement. The second region of the J-segment is adjacent to and 3′ to a J-recombination signal sequence (J-RSS) of said J-segment, and the second region of the J-segment can be at least 10 base pairs in length. In some embodiments, the 3′ end of the J-segment primers are placed at least 10 base pairs downstream of the J-RSS. In certain embodiments, as in TCR Jβ gene segments, the first region of the J-segment includes a unique four base tag at positions +11 through +14 downstream of the RSS site. In other embodiments, the J-segment deletions are 4 bp+/−2.5 bp in length, and the J-segment primers are placed at least 4 bp downstream of the J-RSS. In some embodiments, the J-segment primer is placed greater than 30 base pairs downstream of the J-RSS.

Further description about the design, placement and positioning of the V-segment primers and J-segment primers, and exemplary primers can be found in U.S. Ser. No. 12/794,507, filed on Jun. 4, 2010, International App. No. PCT/US2010/037477, filed on Jun. 4, 2010, and U.S. Ser. No. 13/217,126, filed on Aug. 24, 2011, and Robins et al., 2009 Blood 114, 4099, which are each incorporated by reference in its entirety.

Multiplex PCR Amplification

A multiplex PCR system can be used to amplify rearranged adaptive immune cell receptor loci from genomic DNA and from synthetic template oligonucleotides, preferably from a CDR3 region. In certain embodiments, the CDR3 region is amplified from a TCRα, TCRβ, TCRγ, or TCRδ CDR3 region, or similarly from an Ig locus, such as a IgH or IgL (lambda or kappa) locus.

In general, a multiplex PCR system comprises a plurality of V-segment forward primers and a plurality of J-segment reverse primers. The plurality of V-segment forward primers can comprise at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25, and in certain embodiments, at least 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, or 39, and in other embodiments 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 65, 70, 75, 80, 85, or more forward primers. Each forward primer specifically hybridizes to or is complementary to a sequence corresponding to one or more V region segments.

For example, illustrative V-segment primers for amplification of the TCRB are shown in SEQ ID NOs: 1-120. Illustrative J-segment primers for TCRB are shown in SEQ ID NOs: 121-146. Illustrative TCRG V-segment primers are provided in SEQ ID NOs: 147-158. Illustrative TCRG J-segment primers are provided in SEQ ID NOs: 159-166. Illustrative TCRA and TCRD V-segment primers are found in SEQ ID NOs: 167-276. Exemplary TCRA and TCRD J-segment primers are found in SEQ ID NOs: 277-406. Illustrative IGH V-segment primers are provided in SEQ ID NOs 407-578. Exemplary IGH J-segment primers are found in SEQ ID NOs: 579-592. Exemplary IGK and IGL V-segment primers are found in SEQ ID NOs: 593-740. Exemplary IGK and IGL J-segment primers are found in SEQ ID NOs: 741-764.

The multiplex PCR system can use at least 3, 4, 5, 6, or 7, 8, 9, 10, 11, 12 or 13 reverse primers. In some embodiments, each reverse primer specifically hybridizes to or is complementary to a sequence corresponding to one or more J region segments. In one embodiment, there is a different J segment primer for each J segment. No one J-segment primer is a universal primer that binds to all J-region segments.

Oligonucleotides that are capable of specifically hybridizing or annealing to a target nucleic acid sequence by nucleotide base complementarity may do so under moderate to high stringency conditions. For purposes of illustration, suitable moderate to high stringency conditions for specific PCR amplification of a target nucleic acid sequence would be between 25 and 80 PCR cycles, with each cycle consisting of a denaturation step (e.g., about 10-30 seconds (s) at least about 95° C.), an annealing step (e.g., about 10-30 s at about 60-68° C.), and an extension step (e.g., about 10-60 s at about 60-72° C.), optionally according to certain embodiments with the annealing and extension steps being combined to provide a two-step PCR. As would be recognized by the skilled person, other PCR reagents may be added or changed in the PCR reaction to increase specificity of primer annealing and amplification, such as altering the magnesium concentration, optionally adding DMSO, and/or the use of blocked primers, modified nucleotides, peptide-nucleic acids, and the like.

In certain embodiments, nucleic acid hybridization techniques may be used to assess hybridization specificity of the primers described herein. Hybridization techniques are well known in the art of molecular biology. For purposes of illustration, suitable moderately stringent conditions for testing the hybridization of a polynucleotide as provided herein with other polynucleotides include prewashing in a solution of 5×SSC, 0.5% SDS, 1.0 mM EDTA (pH 8.0); hybridizing at 50° C.-60° C., 5×SSC, overnight; followed by washing twice at 65° C. for 20 minutes with each of 2×, 0.5× and 0.2×SSC containing 0.1% SDS. One skilled in the art will understand that the stringency of hybridization can be readily manipulated, such as by altering the salt content of the hybridization solution and/or the temperature at which the hybridization is performed. For example, in another embodiment, suitable highly stringent hybridization conditions include those described above, with the exception that the temperature of hybridization is increased, e.g., to 60° C.-65° C. or 65° C.-70° C.

In certain embodiments, the primers are designed not to cross an intron/exon boundary. The forward primers in certain embodiments anneal to the V segments in a region of relatively strong sequence conservation between V segments so as to maximize the conservation of sequence among these primers. Accordingly, this minimizes the potential for differential annealing properties of each primer, and so that the amplified region between V- and J-segment primers contains sufficient TCR or Ig V sequence information to identify the specific V gene segment used. In one embodiment, the J-segment primers hybridize with a conserved element of the J segment, and have similar annealing strength. In one particular embodiment, the J segment primers anneal to the same conserved framework region motif.

Oligonucleotides (e.g., primers) can be prepared by any suitable method, including direct chemical synthesis by a method such as the phosphotriester method of Narang et al., 1979, Meth. Enzymol. 68:90-99; the phosphodiester method of Brown et al., 1979, Meth. Enzymol. 68:109-151; the diethylphosphoramidite method of Beaucage et al., 1981, Tetrahedron Lett. 22:1859-1862; and the solid support method of U.S. Pat. No. 4,458,066, each incorporated herein by reference. A review of synthesis methods of conjugates of oligonucleotides and modified nucleotides is provided in Goodchild, 1990, Bioconjugate Chemistry 1(3): 165-187, incorporated herein by reference.

High Throughput Sequencing

Sequencing Oligonucleotides

In one embodiment, the V-segment primers and J-segment primers of the invention include a second subsequence situated at their 5′ ends that include a universal adaptor sequence complementary to and that can hybridize to sequencing adaptor sequences for use in a DNA sequencer, such as Illumina.

In certain embodiments, the J-region encoding gene segments each have a unique sequence-defined identifier tag of 2, 3, 4, 5, 6, 7, 8, 9, 10 or about 15, 20 or more nucleotides, situated at a defined position relative to a RSS site. For example, a four-base tag may be used, in the Jβ-region encoding segment of amplified TCRβ CDR3-encoding regions, at positions +11 through +14 downstream from the RSS site. However, these and related embodiments need not be so limited and also contemplate other relatively short nucleotide sequence-defined identifier tags that may be detected in J-region encoding gene segments and defined based on their positions relative to an RSS site. These may vary between different adaptive immune receptor encoding loci.

The recombination signal sequence (RSS) consists of two conserved sequences (heptamer, 5′-CACAGTG-3′, and nonamer, 5′-ACAAAAACC-3′), separated by a spacer of either 12+/−1 bp (“12-signal”) or 23+/−1 bp (“23-signal”). A number of nucleotide positions have been identified as important for recombination including the CA dinucleotide at position one and two of the heptamer, and a C at heptamer position three has also been shown to be strongly preferred as well as an A nucleotide at positions 5, 6, 7 of the nonamer. (Ramsden et. al 1994; Akamatsu et. al. 1994; Hesse et. al. 1989). Mutations of other nucleotides have minimal or inconsistent effects. The spacer, although more variable, also has an impact on recombination, and single-nucleotide replacements have been shown to significantly impact recombination efficiency (Fanning et. al. 1996, Larijani et. al 1999; Nadel et. al. 1998). Criteria have been described for identifying RSS polynucleotide sequences having significantly different recombination efficiencies (Ramsden et. al 1994; Akamatsu et. al. 1994; Hesse et. al. 1989 and Cowell et. al. 1994). Accordingly, the sequencing oligonucleotides may hybridize adjacent to a four base tag within the amplified J-encoding gene segments at positions +11 through +14 downstream of the RSS site. For example, sequencing oligonucleotides for TCRB may be designed to anneal to a consensus nucleotide motif observed just downstream of this “tag”, so that the first four bases of a sequence read will uniquely identify the J-encoding gene segment. Exemplary sequencing oligonucleotide sequences are found, for example in SEQ ID NOs: 765-786.

The information used to assign identities to the J- and V-encoding segments of a sequence read is entirely contained within the amplified sequence, and does not rely upon the identity of the PCR primers. In particular, the methods described herein allow for the amplification of all possible V-J combinations at a TCR or Ig locus and sequencing of the individual amplified molecules allows for the identification and quantitation of the rearranged DNA encoding the CDR3 regions. The diversity of the adaptive immune cells of a given sample can be inferred from the sequences generated using the methods and algorithms described herein.

High Throughput Sequencing Methods

Methods of the invention further comprise sequencing the amplified adaptive immune receptor encoding DNA molecules that are produced. Sequencing can performed on amplicon products produced from a biological sample comprising adaptive immune cells, and/or of the synthetic template oligonucleotides that are described below.

In one embodiment, sequencing involves using a set of sequencing oligonucleotides (adaptor sequences) that hybridize to sequencing oligonucleotide sequences within the amplified DNA molecules or the synthetic template oligonucleotides that are described below.

Sequencing may be performed using any of a variety of available high through-put single molecule sequencing machines and systems. Illustrative sequence systems include sequence-by-synthesis systems such as the Illumina Genome Analyzer, the Illumina MiSeq, and associated instruments (Illumina, Inc., San Diego, Calif.), Helicos Genetic Analysis System (Helicos BioSciences Corp., Cambridge, Mass.), Pacific Biosciences PacBio RS (Pacific Biosciences, Menlo Park, Calif.), or other systems having similar capabilities. Sequencing is achieved using a set of sequencing oligonucleotides that hybridize to a defined region within the amplified DNA molecules. The sequencing oligonucleotides are designed such that the V- and J-encoding gene segments can be uniquely identified by the sequences that are generated, based on the present disclosure and in view of known adaptive immune receptor gene sequences that appear in publicly available databases.

In certain embodiments, at least 30, 40, 50, 60, 70, 80, 90, 100, 101-150, 151-200, 201-300, 301-500, and not more than 1000 contiguous nucleotides of the amplified adaptive immune receptor encoding DNA molecules are sequenced. In some embodiments, the amplicons and synthetic template oligonucleotides that are sequenced are less than 600 bps in length. In further embodiments, the resulting sequencing reads are approximately 130 bps in length. In yet further embodiments, approximately 30 million sequencing reads are produced per sequencing assay.

Compositions and methods for the sequencing of rearranged adaptive immune receptor gene sequences and for adaptive immune receptor clonotype determination are described further in Robins et al., 2009 Blood 114, 4099; Robins et al., 2010 Sci. Translat. Med. 2:47ra64; Robins et al., 2011 J. Immunol. Meth. doi:10.1016/j.jim.2011.09.001; Sherwood et al. 2011 Sci. Translat. Med. 3:90ra61; U.S. Ser. No. 13/217,126 (US Pub. No. 2012/0058902), U.S. Ser. No. 12/794,507 (US Pub. No. 2010/0330571), WO/2010/151416, WO/2011/106738 (PCT/US2011/026373), WO2012/027503 (PCT/US2011/049012), U.S. Ser. No. 61/550,311, and U.S. Ser. No. 61/569,118, which are incorporated by reference their entireties.

In certain embodiments, the amplified J-region encoding gene segments may each have a unique sequence-defined identifier tag of 2, 3, 4, 5, 6, 7, 8, 9, 10 or about 15, 20 or more nucleotides, situated at a defined position relative to a RSS site. For example, a four-base tag may be used, in the Jβ-region encoding segment of amplified TCRβ CDR3-encoding regions, at positions +11 through +14 downstream from the RSS site. However, these and related embodiments need not be so limited and also contemplate other relatively short nucleotide sequence-defined identifier tags that may be detected in J-region encoding gene segments and defined based on their positions relative to an RSS site. These may vary between different adaptive immune receptor encoding loci.

The recombination signal sequence (RSS) consists of two conserved sequences (heptamer, 5′-CACAGTG-3′, and nonamer, 5′-ACAAAAACC-3′), separated by a spacer of either 12+/−1 bp (“12-signal”) or 23+/−1 bp (“23-signal”). A number of nucleotide positions have been identified as important for recombination including the CA dinucleotide at position one and two of the heptamer, and a C at heptamer position three has also been shown to be strongly preferred as well as an A nucleotide at positions 5, 6, 7 of the nonamer. (Ramsden et. al 1994; Akamatsu et. al. 1994; Hesse et. al. 1989). Mutations of other nucleotides have minimal or inconsistent effects. The spacer, although more variable, also has an impact on recombination, and single-nucleotide replacements have been shown to significantly impact recombination efficiency (Fanning et. al. 1996, Larijani et. al 1999; Nadel et. al. 1998). Criteria have been described for identifying RSS polynucleotide sequences having significantly different recombination efficiencies (Ramsden et. al 1994; Akamatsu et. al. 1994; Hesse et. al. 1989 and Cowell et. al. 1994). Accordingly, the sequencing oligonucleotides may hybridize adjacent to a four base tag within the amplified J-encoding gene segments at positions +11 through +14 downstream of the RSS site. For example, sequencing oligonucleotides for TCRB may be designed to anneal to a consensus nucleotide motif observed just downstream of this “tag”, so that the first four bases of a sequence read will uniquely identify the J-encoding gene segment. Exemplary TCRB J primers are found in SEQ ID NOs:121-146 (TCRB J-segment reverse primers (gene specific) and TCRB J-segment reverse primers with an universal adaptor sequence.

The information used to assign identities to the J- and V-encoding segments of a sequence read is entirely contained within the amplified sequence, and does not rely upon the identity of the PCR primers. In particular, the methods described herein allow for the amplification of all possible V-J combinations at a TCR or Ig locus and sequencing of the individual amplified molecules allows for the identification and quantitation of the rearranged DNA encoding the CDR3 regions. The diversity of the adaptive immune cells of a given sample can be inferred from the sequences generated using the methods and algorithms described herein. One surprising advantage provided in certain preferred embodiments by the compositions and methods of the present disclosure was the ability to amplify successfully all possible V-J combinations of an adaptive immune cell receptor locus in a single multiplex PCR reaction.

In certain embodiments, the sequencing oligonucleotides described herein may be selected such that promiscuous priming of a sequencing reaction for one J-encoding gene segment by an oligonucleotide specific to another distinct J-encoding gene segment generates sequence data starting at exactly the same nucleotide as sequence data from the correct sequencing oligonucleotide. In this way, promiscuous annealing of the sequencing oligonucleotides does not impact the quality of the sequence data generated.

The average length of the CDR3-encoding region, for the TCR, defined as the nucleotides encoding the TCR polypeptide between the second conserved cysteine of the V segment and the conserved phenylalanine of the J segment, is 35+/−3 nucleotides. Accordingly and in certain embodiments, PCR amplification using V-segment primers and J-segment primers that start from the J segment tag of a particular TCR or IgH J region (e.g., TCR Jβ, TCR Jγ or IgH JH as described herein) will nearly always capture the complete V-D-J junction in a 50 base pair read. The average length of the IgH CDR3 region, defined as the nucleotides between the conserved cysteine in the V segment and the conserved phenylalanine in the J segment, is less constrained than at the TCRI3 locus, but will typically be between about 10 and about 70 nucleotides. Accordingly and in certain embodiments, PCR amplification using V-segment primers and J-segment primers that start from the IgH J segment tag will capture the complete V-D-J junction in a 100 base pair read.

PCR primers that anneal to and support polynucleotide extension on mismatched template sequences are referred to as promiscuous primers. In certain embodiments, the TCR and Ig J-segment reverse PCR primers may be designed to minimize overlap with the sequencing oligonucleotides, in order to minimize promiscuous priming in the context of multiplex PCR. In one embodiment, the TCR and Ig J-segment reverse primers may be anchored at the 3′ end by annealing to the consensus splice site motif, with minimal overlap of the sequencing primers. Generally, the TCR and Ig V and J-segment primers may be selected to operate in PCR at consistent annealing temperatures using known sequence/primer design and analysis programs under default parameters. For the sequencing reaction, exemplary IGH J primers used for sequencing are found in SEQ ID NOs: 579-592 (showing IGH J-segment reverse primers (gene specific) and IGH J-segment reverse primers with a universal adaptor sequence.

Processing Sequence Data

As presently disclosed, there are also provided methods for analyzing the sequences of the diverse pool of rearranged CDR3-encoding regions that are generated using the compositions and methods that are described herein. In particular, an algorithm is provided to correct for PCR bias, sequencing and PCR errors and for estimating true distribution of specific clonotypes (e.g., a TCR or Ig having a uniquely rearranged CDR3 sequence) in a sample. A preferred algorithm is described in further detail herein. As would be recognized by the skilled person, the algorithms provided herein may be modified appropriately to accommodate particular experimental or clinical situations.

The use of a PCR step to amplify the TCR or Ig CDR3 regions prior to sequencing could potentially introduce a systematic bias in the inferred relative abundance of the sequences, due to differences in the efficiency of PCR amplification of CDR3 regions utilizing different V and J gene segments. As discussed in more detail in the Examples, each cycle of PCR amplification potentially introduces a bias of average magnitude 1.51/15=1.027. Thus, the 25 cycles of PCR introduces a total bias of average magnitude 1.02725=1.95 in the inferred relative abundance of distinct CDR3 region sequences.

Sequenced reads are filtered for those including CDR3 sequences. Sequencer data processing involves a series of steps to remove errors in the primary sequence of each read, and to compress the data. A complexity filter removes approximately 20% of the sequences that are misreads from the sequencer. Then, sequences were required to have a minimum of a six base match to both one of the TCR or Ig J-regions and one of V-regions. Applying the filter to the control lane containing phage sequence, on average only one sequence in 7-8 million passed these steps. Finally, a nearest neighbor algorithm is used to collapse the data into unique sequences by merging closely related sequences, in order to remove both PCR error and sequencing error.

Analyzing the data, the ratio of sequences in the PCR product are derived working backward from the sequence data before estimating the true distribution of clonotypes (e.g., unique clonal sequences) in the blood. For each sequence observed a given number of times in the data herein, the probability that that sequence was sampled from a particular size PCR pool is estimated. Because the CDR3 regions sequenced are sampled randomly from a massive pool of PCR products, the number of observations for each sequence are drawn from Poisson distributions. The Poisson parameters are quantized according to the number of T cell genomes that provided the template for PCR. A simple Poisson mixture model both estimates these parameters and places a pairwise probability for each sequence being drawn from each distribution. This is an expectation maximization method which reconstructs the abundances of each sequence that was drawn from the blood.

To estimate the total number of unique adaptive immune receptor CDR3 sequences that are present in a sample, a computational approach employing the “unseen species” formula may be employed (Efron and Thisted, 1976 Biometrika 63, 435-447). This approach estimates the number of unique species (e.g., unique adaptive immune receptor sequences) in a large, complex population (e.g., a population of adaptive immune cells such as T cells or B cells), based on the number of unique species observed in a random, finite sample from a population (Fisher et al., 1943 J. Anim. Ecol. 12:42-58; Ionita-Laza et al., 2009 Proc. Nat. Acad. Sci. USA 106:5008). The method employs an expression that predicts the number of “new” species that would be observed if a second random, finite and identically sized sample from the same population were to be analyzed. “Unseen” species refers to the number of new adaptive immune receptor sequences that would be detected if the steps of amplifying adaptive immune receptor-encoding sequences in a sample and determining the frequency of occurrence of each unique sequence in the sample were repeated an infinite number of times. By way of non-limiting theory, it is operationally assumed for purposes of these estimates that adaptive immune cells (e.g., T cells, B cells) circulate freely in the anatomical compartment of the subject that is the source of the sample from which diversity is being estimated (e.g., blood, lymph, etc.).

To apply this formula, unique adaptive immune receptors (e.g., TCRβ, TCRα, TCRγ, TCRδ, IgH) clonotypes takes the place of species. The mathematical solution provides that for S, the total number of adaptive immune receptors having unique sequences (e.g., TCRβ, TCRγ, IgH “species” or clonotypes, which may in certain embodiments be unique CDR3 sequences), a sequencing experiment observes xs copies of sequence s. For all of the unobserved clonotypes, xs equals 0, and each TCR or Ig clonotype is “captured” in the course of obtaining a random sample (e.g., a blood draw) according to a Poisson process with parameter λs. The number of T or B cell genomes sequenced in the first measurement is defined as 1, and the number of T or B cell genomes sequenced in the second measurement is defined as t.

Because there are a large number of unique sequences, an integral is used instead of a sum. If G(λ) is the empirical distribution function of the parameters λ1, . . . , λS, and nx is the number of clonotypes (e.g., unique TCR or Ig sequences, or unique CDR3 sequences) observed exactly x times, then the total number of clonotypes, i.e., the measurement of diversity E, is given by the following formula (I):

E(nx)=S0(e-λλxx!)dG(λ).(I)

Accordingly, formula (I) may be used to estimate the total diversity of species in the entire source from which the identically sized samples are taken. Without wishing to be bound by theory, the principle is that the sampled number of clonotypes in a sample of any given size contains sufficient information to estimate the underlying distribution of clonotypes in the whole source. The value for Δ(t), the number of new clonotypes observed in a second measurement, may be determined, preferably using the following equation (II):

Δ(t)=xE(nx)msmt1+msmt2-xE(nx)msmt1=S0e-λ(1-e-λt)dG(λ)(II)

in which msmt1 and msmt2 are the number of clonotypes from measurements 1 and 2, respectively. Taylor expansion of 1−e−λt and substitution into the expression for Δ(t) yields:

Δ(t)=E(x1)t−E(x2)t2+E(x3)t3− . . . ,  (III)

which can be approximated by replacing the expectations (E(nx)) with the actual numbers sequences observed exactly x times in the first sample measurement. The expression for Δ(t) oscillates widely as t goes to infinity, so Δ(t) is regularized to produce a lower bound for Δ(∞), for example, using the Euler transformation (Efron et al., 1976 Biometrika 63:435).

According to certain herein expressly disclosed embodiments, there are also presently provided methods in which the degree of clonality of adaptive immune cells that are present in a sample, such as a sample that comprises a mixture of cells only some of which are adaptive immune cells, can be determined advantageously without the need for cell sorting or for DNA sequencing. These and related embodiments overcome the challenges of efficiency, time and cost that, prior to the present disclosure, have hindered the ability to determine whether adaptive immune cell presence in a sample (e.g., TIL) is monoclonal or oligoclonal (e.g., whether all TILs are the progeny of one or a relatively limited number of adaptive immune cells), or whether instead adaptive immune cell presence in the sample is polyclonal (e.g., TILs are the progeny of a relatively large number of adaptive immune cells).

According to non-limiting theory, these embodiments exploit current understanding in the art (also described above) that once an adaptive immune cell (e.g., a T or B lymphocyte) has rearranged its adaptive immune receptor-encoding (e.g., TCR or Ig) genes, its progeny cells possess the same adaptive immune receptor-encoding gene rearrangement, thus giving rise to a clonal population that can be identified by the presence therein of rearranged CDR3-encoding V- and J-gene segments that may be amplified by a specific pairwise combination of V- and J-specific oligonucleotide primers as herein disclosed.

Synthetic Template Oligonucleotide Compositions for Use in Quantifying Input Genomes from Adaptive Immune Cells, and Determining Relative Representation of Adaptive Immune Cells

Synthetic Template Compositions Useful for Quantifying Numbers of Input Molecules in a Sample

Synthetic template oligonucleotides can be designed to quantify a number of input molecules in a biological sample. As used herein “synthetic template” means an oligonucleotide containing sequences which include sequences substantially identical to biological sequences (i.e. TCR or IG V, J or C segments or genomic control regions) in addition to non-naturally occurring sequences (i.e. barcodes, randomers, adaptors, etc.). The full nucleotide sequence of synthetic templates, therefore, do not occur in nature and are, instead, laboratory designed and made sequences. A ratio of the number of input synthetic template oligonucleotide molecules in a sample compared to the number of total output sequencing reads of synthetic template oligonucleotides (sequenced from synthetic template amplicons) in the sample is determined. In one embodiment, a limiting dilution of synthetic template oligonucleotides (which allows for the determination of the number of total synthetic template oligonucleotide molecules present by measuring the number of unique synthetic template oligonucleotide sequences observed) is added to a biological sample for multiplex PCR, and by assuming the same ratio holds for biological as synthetic templates, the ratio is used to determine the number of rearranged T or B cell receptor molecules, and thus the number of T or B cells, in the biological sample.

The invention comprises a synthetic template composition comprising a plurality of template oligonucleotides of general formula (I):

5′-U1-B1-V-B2-J-B3-U2-3′  (I).

The constituent template oligonucleotides are diverse with respect to the nucleotide sequences of the individual template oligonucleotides.

In one embodiment, U1 and U2 are each either nothing or each comprise an oligonucleotide having, independently, a sequence that is selected from (i) a universal adaptor oligonucleotide sequence, and (ii) a sequencing platform-specific oligonucleotide sequence that is linked to and positioned 5′ to the universal adaptor oligonucleotide sequence.

B1, B2, and B3 can each be independently either nothing or each comprise an oligonucleotide “B” that comprises an oligonucleotide barcode sequence of 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900 or 1000 contiguous nucleotides (including all integer values therebetween). In some embodiments, B1, B2, and B3 can each comprise a unique oligonucleotide sequence that uniquely identifies, or identifies as a paired combination, (i) the unique V oligonucleotide sequence of the template oligonucleotide and (ii) the unique J oligonucleotide sequence of the template oligonucleotide.

The relative positioning of the barcode oligonucleotides B1, B2, and B3 and universal adaptors U1 and U2 advantageously permits rapid identification and quantification of the amplification products of a given unique template oligonucleotide by short sequence reads and paired-end sequencing on automated DNA sequencers (e.g., Illumina HiSeq™ or Illumina MiSEQ®, or GeneAnalyzer™-2, Illumina Corp., San Diego, Calif.). In particular, these and related embodiments permit rapid high-throughput determination of specific combinations of a V-segment sequence and a J-segment sequence that are present in an amplification product, thereby to characterize the relative amplification efficiency of each V-specific primer and each J-specific primer that may be present in a primer set, which is capable of amplifying rearranged TCR or BCR encoding DNA in a sample. Verification of the identities and/or quantities of the amplification products may be accomplished by longer sequence reads, optionally including sequence reads that extend to B2.

V can be either nothing or a polynucleotide comprising at least 20, 30, 60, 90, 120, 150, 180, or 210, and not more than 1000, 900, 800, 700, 600 or 500 contiguous nucleotides of a DNA sequence. In some embodiments, the DNA sequence is of an adaptive immune receptor variable (V) region encoding gene sequence, or the complement thereof, and in each of the plurality of template oligonucleotide sequences V comprises a unique oligonucleotide sequence.

J can be either nothing or a polynucleotide comprising at least 15-30, 31-60, 61-90, 91-120, or 120-150, and not more than 600, 500, 400, 300 or 200 contiguous nucleotides of a DNA sequence. In some embodiments, the DNA sequence is of an adaptive immune receptor joining (J) region encoding gene sequence, or the complement thereof, and in each of the plurality of template oligonucleotide sequences J comprises a unique oligonucleotide sequence.

In constructing the “V” and “J” portions of the synthetic template oligonucleotides of formula I, various adaptive immune receptor variable (V) region and joining (J) region gene sequences can be used. A large number of V and J region gene sequences are known as nucleotide and/or amino acid sequences, including non-rearranged genomic DNA sequences of TCR and Ig loci, and productively rearranged DNA sequences at such loci and their encoded products, and also including pseudogenes at these loci, and also including related orphons. See, e.g., U.S. Ser. No. 13/217,126; U.S. Ser. No. 12/794,507; PCT/US2011/026373; PCT/US2011/049012, which are incorporated by reference in their entireties. Moreover, genomic sequences for TCR and BCR V region genes of humans and other species are known and available from public databases such as Genbank. V region gene sequences include polynucleotide sequences that encode the products of expressed, rearranged TCR and BCR genes and also include polynucleotide sequences of pseudogenes that have been identified in the V region loci. The diverse V polynucleotide sequences that may be incorporated into the presently disclosed templates of general formula (I) may vary widely in length, in nucleotide composition (e.g., GC content), and in actual linear polynucleotide sequence, and are known, for example, to include “hot spots” or hypervariable regions that exhibit particular sequence diversity. These and other sequences known to the art may be used according to the present disclosure for the design and production of template oligonucleotides to be included in the presently provided template composition for standardizing amplification efficiency of an oligonucleotide primer set, and for the design and production of the oligonucleotide primer set that is capable of amplifying rearranged DNA encoding TCR or Ig polypeptide chains, which rearranged DNA may be present in a biological sample comprising lymphoid cell DNA.

The entire polynucleotide sequence of each polynucleotide V in general formula (I) can, but need not, consist exclusively of contiguous nucleotides from each distinct V gene. For example and according to certain embodiments, in the template composition described herein, each polynucleotide V of formula (I) need only have at least a region comprising a unique V oligonucleotide sequence that is found in one V gene and to which a single V region primer in the primer set can specifically anneal. Thus, the V polynucleotide of formula (I) may comprise all or any prescribed portion (e.g., at least 15, 20, 30, 60, 90, 120, 150, 180 or 210 contiguous nucleotides, or any integer value therebetween) of a naturally occurring V gene sequence (including a V pseudogene sequence), so long as at least one unique V oligonucleotide sequence region (e.g., the primer annealing site) is included that is not included in any other template V polynucleotide.

In some embodiments, the plurality of V polynucleotides that are present in the synthetic template composition have lengths that simulate the overall lengths of known, naturally occurring V gene nucleotide sequences, even where the specific nucleotide sequences differ between the template V region and any naturally occurring V gene. The V region lengths in the synthetic templates can differ from the lengths of naturally occurring V gene sequences by no more than 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20 percent. Optionally and according to certain embodiments, the V polynucleotide of the herein described synthetic template oligonucleotide includes a stop codon at or near the 3′ end of V in general formula (I).

The V polynucleotide in formula (I) may thus, in certain embodiments, comprise a nucleotide sequence having a length that is the same or similar to that of the length of a typical V gene from its start codon to its CDR3 encoding region and may, but need not, include a nucleotide sequence that encodes the CDR3 region. CDR3 encoding nucleotide sequences and sequence lengths may vary considerably and have been characterized by several different numbering schemes (e.g., Lefranc, 1999 The Immunologist 7:132; Kabat et al., 1991 In: Sequences of Proteins of Immunological Interest, NIH Publication 91-3242; Chothia et al., 1987 J. Mol. Biol. 196:901; Chothia et al., 1989 Nature 342:877; Al-Lazikani et al., 1997 J. Mol. Biol. 273:927; see also, e.g., Rock et al., 1994 J. Exp. Med. 179:323; Saada et al., 2007 Immunol. Cell Biol. 85:323).

Briefly, the CDR3 region typically spans the polypeptide portion extending from a highly conserved cysteine residue (encoded by the trinucleotide codon TGY; Y=T or C) in the V segment to a highly conserved phenylalanine residue (encoded by TTY) in the J segment of TCRs, or to a highly conserved tryptophan (encoded by TGG) in IGH. More than 90% of natural, productive rearrangements in the TCRB locus have a CDR3 encoding length by this criterion of between 24 and 54 nucleotides, corresponding to between 9 and 17 encoded amino acids. The CDR3 lengths of the presently disclosed synthetic template oligonucleotides should, for any given TCR or BCR locus, fall within the same range as 95% of naturally occurring rearrangements. Thus, for example, in a synthetic template composition described herein, the CDR3 encoding portion of the V polynucleotide cab has a length of from 24 to 54 nucleotides, including every integer therebetween. The numbering schemes for CDR3 encoding regions described above denote the positions of the conserved cysteine, phenylalanine and tryptophan codons, and these numbering schemes may also be applied to pseudogenes in which one or more codons encoding these conserved amino acids may have been replaced with a codon encoding a different amino acid. For pseudogenes which do not use these conserved amino acids, the CDR3 length may be defined relative to the corresponding position at which the conserved residue would have been observed absent the substitution, according to one of the established CDR3 sequence position numbering schemes referenced above.

The entire polynucleotide sequence of each polynucleotide J in general formula (I) may, but need not, consist exclusively of contiguous nucleotides from each distinct J gene. For example and according to certain embodiments, in the template composition described herein, each polynucleotide J of formula (I) need only have at least a region comprising a unique J oligonucleotide sequence that is found in one J gene and to which a single V region primer in the primer set can specifically anneal. Thus, the V polynucleotide of formula (I) may comprise all or any prescribed portion (e.g., at least 15, 20, 30, 60, 90, 120, 150, 180 or 210 contiguous nucleotides, or any integer value therebetween) of a naturally occurring V gene sequence (including a V pseudogene sequence) so long as at least one unique V oligonucleotide sequence region (the primer annealing site) is included that is not included in any other template J polynucleotide.

It may be preferred in certain embodiments that the plurality of J polynucleotides that are present in the herein described template composition have lengths that simulate the overall lengths of known, naturally occurring J gene nucleotide sequences, even where the specific nucleotide sequences differ between the template J region and any naturally occurring J gene. The J region lengths in the herein described templates may differ from the lengths of naturally occurring J gene sequences by no more than 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20 percent.

The J polynucleotide in formula (I) may thus, in certain embodiments, comprise a nucleotide sequence having a length that is the same or similar to that of the length of a typical naturally occurring J gene and may, but need not, include a nucleotide sequence that encodes the CDR3 region, as discussed above.

Genomic sequences for TCR and BCR J region genes of humans and other species are known and available from public databases such as Genbank; J region gene sequences include polynucleotide sequences that encode the products of expressed and unexpressed rearranged TCR and BCR genes. The diverse J polynucleotide sequences that may be incorporated into the presently disclosed templates of general formula (I) may vary widely in length, in nucleotide composition (e.g., GC content), and in actual linear polynucleotide sequence.

Alternatives to the V and J sequences described herein, for use in construction of the herein described template oligonucleotides and/or V-segment and J-segment oligonucleotide primers, may be selected by a skilled person based on the present disclosure using knowledge in the art regarding published gene sequences for the V- and J-encoding regions of the genes for each TCR and Ig subunit. Reference Genbank entries for human adaptive immune receptor sequences include: TCRα: (TCRA/D): NC_000014.8 (chr14:22090057 . . . 23021075); TCRβ: (TCRB): NC_000007.13 (chr7:141998851 . . . 142510972); TCRγ: (TCRG): NC_000007.13 (chr7:38279625 . . . 38407656); immunoglobulin heavy chain, IgH (IGH): NC_000014.8 (chr14: 106032614 . . . 107288051); immunoglobulin light chain-kappa, IgLκ (IGK): NC_000002.11 (chr2: 89156874 . . . 90274235); and immunoglobulin light chain-lambda, IgLλ (IGL): NC_000022.10 (chr22: 22380474 . . . 23265085). Reference Genbank entries for mouse adaptive immune receptor loci sequences include: TCRβ: (TCRB): NC_000072.5 (chr6: 40841295 . . . 41508370), and immunoglobulin heavy chain, IgH (IGH): NC_000078.5 (chr12:114496979 . . . 117248165).

Template and primer design analyses and target site selection considerations can be performed, for example, using the OLIGO primer analysis software and/or the BLASTN 2.0.5 algorithm software (Altschul et al., Nucleic Acids Res. 1997, 25(17):3389-402), or other similar programs available in the art.

Accordingly, based on the present disclosure and in view of these known adaptive immune receptor gene sequences and oligonucleotide design methodologies, for inclusion in the instant template oligonucleotides those skilled in the art can design a plurality of V region-specific and J region-specific polynucleotide sequences that each independently contain oligonucleotide sequences that are unique to a given V and J gene, respectively. Similarly, from the present disclosure and in view of known adaptive immune receptor sequences, those skilled in the art can also design a primer set comprising a plurality of V region-specific and J region-specific oligonucleotide primers that are each independently capable of annealing to a specific sequence that is unique to a given V and J gene, respectively, whereby the plurality of primers is capable of amplifying substantially all V genes and substantially all J genes in a given adaptive immune receptor-encoding locus (e.g., a human TCR or IgH locus). Such primer sets permit generation, in multiplexed (e.g., using multiple forward and reverse primer pairs) PCR, of amplification products that have a first end that is encoded by a rearranged V region-encoding gene segment and a second end that is encoded by a J region-encoding gene segment.

Typically and in certain embodiments, such amplification products may include a CDR3-encoding sequence although the invention is not intended to be so limited and contemplates amplification products that do not include a CDR3-encoding sequence. The primers may be preferably designed to yield amplification products having sufficient portions of V and J sequences and/or of V-J barcode (B) sequences as described herein, such that by sequencing the products (amplicons), it is possible to identify on the basis of sequences that are unique to each gene segment (i) the particular V gene, and (ii) the particular J gene in the proximity of which the V gene underwent rearrangement to yield a functional adaptive immune receptor-encoding gene. Typically, and in preferred embodiments, the PCR amplification products will not be more than 600 base pairs in size, which according to non-limiting theory will exclude amplification products from non-rearranged adaptive immune receptor genes. In certain other preferred embodiments the amplification products will not be more than 500, 400, 300, 250, 200, 150, 125, 100, 90, 80, 70, 60, 50, 40, 30 or 20 base pairs in size, such as may advantageously provide rapid, high-throughput quantification of sequence-distinct amplicons by short sequence reads.

In one embodiment of formula I, V is a polynucleotide sequence that encodes at least 10-70 contiguous amino acids of an adaptive immune receptor V-region, or the complement thereof; J is a polynucleotide sequence that encodes at least 5-30 contiguous amino acids of an adaptive immune receptor J-region, or the complement thereof; U1 and U2 are each either nothing or comprise an oligonucleotide comprising a nucleotide sequence that is selected from (i) a universal adaptor oligonucleotide sequence, and (ii) a sequencing platform-specific oligonucleotide sequence that is linked to and positioned 5′ to the universal adaptor oligonucleotide sequence; B1, B2, and B3 are each independently either nothing or each comprise an oligonucleotide B that comprises an oligonucleotide barcode sequence of 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20 contiguous nucleotides, wherein in each of the plurality of oligonucleotide sequences, B comprises a unique oligonucleotide sequence that uniquely identifies, as a paired combination, (i) the unique V oligonucleotide sequence and (ii) the unique J oligonucleotide sequence.

In another embodiment of formula (I), V is a polynucleotide sequence of at least 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400 or 450 and not more than 1000, 900, 800, 700, 600 or 500 contiguous nucleotides of an adaptive immune receptor (e.g., TCR or BCR) variable (V) region gene sequence, or the complement thereof, and in each of the plurality of oligonucleotide sequences V comprises a unique oligonucleotide sequence.

Additional description about synthetic template oligonucleotides can be found in International Application No. PCT/US2013/040221, filed May 8, 2013, which is incorporated by reference in its entirety.

FIG. 1A illustrates one example of a synthetic template oligonucleotide, according to an embodiment of the invention. In one embodiment, a synthetic template oligonucleotide comprises the following regions (left to right, as shown in FIG. 1): a universal primer sequence (UA) (102), a template-specific barcode (BC) (104), a sequence comprising a portion of or all of a unique adaptive immune receptor variable (V) region encoding gene sequence (V gene) (106), a synthetic template internal marker (IM) (108), a repeat of the barcode (BC) (104), a repeat of the internal marker (IM) (108), a sequence comprising a portion of or all of a unique adaptive immune receptor variable (J) region encoding gene sequence (J gene) (110), a third repeat of the barcode (BC) (104), and a reverse universal primer sequence (UB) (112). Each synthetic template oligonucleotide includes a unique adaptive immune receptor variable (V) region encoding gene sequence and unique adaptive immune receptor joining (J) region encoding gene sequence. The combination of V and J sequences on the synthetic template oligonucleotides are the same as those found in biological molecules comprising unique combinations of rearranged V and J sequences in the sample.

In one example, the synthetic template oligonucleotide can be a 495 bp sequence comprising a universal primer sequence (UA) (102), a 16 bp template-specific barcode (BC) (104), a 300 bp adaptive immune receptor variable (V) region encoding gene sequence (V gene) (106), a 9 bp synthetic template internal marker (IM) (108), a repeat of the barcode (BC) (104), a repeat of the internal marker (IM) (108), a 100 bp adaptive immune receptor variable (J) region encoding gene sequence (J gene) (110), a third repeat of the barcode (BC) (104), and a reverse universal primer sequence (UB) (112). Various lengths of the sequences and order of the regions can be used in designing the synthetic template oligonucleotides, as known by one skilled in the art.

The synthetic template oligonucleotides of Formula I can also include adaptor sequences. The adaptor sequences can be added to the synthetic template oligonucleotides by designing primers that include adaptor sequences at their 5′-ends and that specifically hybridize to the adaptor UA and UB regions on the synthetic template oligonucleotides (see FIG. 1(A). An example of an adaptor sequence is an Illumina adaptor sequence, as described in the section “Adaptors” below.

In one embodiment, the resulting synthetic template oligonucleotide amplicons have the structure of general formula I and can include an adaptor sequence or adaptor sequences (Illumina sequence), such that the sequence of the synthetic template oligonucleotide comprises the following: an adaptor sequence, a universal primer sequence (UA) (102), a template-specific barcode (BC) (104), an adaptive immune receptor variable (V) region encoding gene sequence (V gene) (106), a synthetic template internal marker (IM) (108), a repeat of the barcode (BC) (104), a repeat of the internal marker (IM) (108), an adaptive immune receptor variable (J) region encoding gene sequence (J gene) (110), a third repeat of the barcode (BC) (104), a reverse universal primer sequence (UB) (112), and a second adaptor sequence.

Number of Synthetic Template Oligonucleotides in Sample

In certain embodiments, the synthetic template composition comprises a plurality of distinct and unique synthetic template oligonucleotides. In one embodiment, the plurality of synthetic template oligonucleotides comprises at least a or at least b unique oligonucleotide sequences, whichever is larger, wherein a is the number of unique adaptive immune receptor V region-encoding gene segments in the subject and b is the number of unique adaptive immune receptor J region-encoding gene segments in the subject, and the composition comprises at least one template oligonucleotide for each unique V polynucleotide and at least one template oligonucleotide for each unique J polynucleotide.

In another embodiment, the plurality of template oligonucleotides comprises at least (a×b) unique oligonucleotide sequences, where a is the number of unique adaptive immune receptor V region-encoding gene segments in the subject and b is the number of unique adaptive immune receptor J region-encoding gene segments in the subject, and the composition comprises at least one template oligonucleotide for every possible combination of a V region-encoding gene segment and a J region-encoding gene segment.

Accordingly, the composition may accommodate at least one occurrence of each unique V polynucleotide sequence and at least one occurrence of each unique J polynucleotide sequence, where in some instances the at least one occurrence of a particular unique V polynucleotide will be present in the same template oligonucleotide in which may be found the at least one occurrence of a particular unique J polynucleotide. Thus, for example, “at least one template oligonucleotide for each unique V polynucleotide and at least one template oligonucleotide for each unique J polynucleotide” may in certain instances refer to a single template oligonucleotide in which one unique V polynucleotide and one unique J polynucleotide are present.

In one embodiment, a is 1 to a number of maximum V gene segments in the mammalian genome of the subject. In another embodiment, b is 1 to a number of maximum J gene segments in the mammalian genome of the subject. In other embodiments, a is 1. In other embodiments, b is 1.

In some embodiments, a can range from 1 V gene segment to 54 V gene segments for TCRA, 1-76 V gene segments for TCRB, 1-15 V gene segments for TCRG, 1-7 V gene segments for TCRD, 1-165 V gene segments for IGH, 1-111 for IGK, or 1-79 V gene segments for IGL. In other embodiments, b can range from 1 J gene segment to 61 J gene segments for TCRA, 1-14 J gene segments for TCRB, 1-5 J gene segments for TCRG, 1-4 gene segments for TCRD, 1-9 J gene segments for IGH, 1-5 J gene segments for IGK, or 1-11 J gene segments for IGL. In certain embodiments, a pool of synthetic template oligonucleotides comprising every possible combination of a V region-encoding gene segment and a J region-encoding gene segment comprises 248 unique synthetic template types for TCRA/D, 858 unique synthetic types for TCRB, 70 unique synthetic template types for TCRG, 1116 unique synthetic template types for IGH, and 370 unique synthetic template types for IGK/L.

The table below lists the number of V gene segments (a) and J gene segments (b) for each human adaptive immune receptor loci, including functional V and J segments.


TABLE 1
Number of V gene segments (a) and J gene segments (b)
V
functional V
J
Functional J
segments *
segments **
segments *
segments **
TCRA
54
45
61
50
TCRB
76
48
14
13
TCRG
15
6
5
5
TCRD
7
7
4
4
IGH
165
51
9
6
IGK
111
44
5
5
IGL
79
33
11
7
* Total variable and joining segment genes
** Variable and joining segment genes with at least one functional allele

In some embodiments, the J polynucleotide of the synthetic template oligonucleotide comprises at least 15-30, 31-60, 61-90, 91-120, or 120-150, and not more than 600, 500, 400, 300 or 200 contiguous nucleotides of an adaptive immune receptor J constant region, or the complement thereof.

The presently contemplated invention is not intended to be so limited, however, such that in certain embodiments, a substantially fewer number of template oligonucleotides may advantageously be used. In these and related embodiments, where a is the number of unique adaptive immune receptor V region-encoding gene segments in a subject and b is the number of unique adaptive immune receptor J region-encoding gene segments in the subject, the minimum number of unique oligonucleotide sequences of which the plurality of synthetic template oligonucleotides is comprised may be determined by whichever is the larger of a and b, so long as each unique V polynucleotide sequence and each unique J polynucleotide sequence is present in at least one synthetic template oligonucleotide in the template composition. Thus, according to certain related embodiments, the template composition may comprise at least one synthetic template oligonucleotide for each unique V polynucleotide, e.g., that includes a single one of each unique V polynucleotide according to general formula (I), and at least one synthetic template oligonucleotide for each unique J polynucleotide, e.g., that includes a single one of each unique J polynucleotide according to general formula (I).

In certain other embodiments, the template composition comprises at least one synthetic template oligonucleotide to which each oligonucleotide amplification primer in an amplification primer set can anneal.

That is, in certain embodiments, the template composition comprises at least one synthetic template oligonucleotide having an oligonucleotide sequence of general formula (I) to which each V-segment oligonucleotide primer can specifically hybridize, and at least one synthetic template oligonucleotide having an oligonucleotide sequence of general formula (I) to which each J-segment oligonucleotide primer can specifically hybridize.

According to such embodiments, the oligonucleotide primer set that is capable of amplifying rearranged DNA encoding one or a plurality of adaptive immune receptors comprises a plurality a′ of unique V-segment oligonucleotide primers and a plurality b′ of unique J-segment oligonucleotide primers. The plurality of a′ V-segment oligonucleotide primers are each independently capable of annealing or specifically hybridizing to at least one polynucleotide encoding an adaptive immune receptor V-region polypeptide or to the complement thereof, wherein each V-segment primer comprises a nucleotide sequence of at least 15 contiguous nucleotides that is complementary to at least one adaptive immune receptor V region-encoding gene segment. The plurality of b′ J-segment oligonucleotide primers are each independently capable of annealing or specifically hybridizing to at least one polynucleotide encoding an adaptive immune receptor J-region polypeptide or to the complement thereof, wherein each J-segment primer comprises a nucleotide sequence of at least 15 contiguous nucleotides that is complementary to at least one adaptive immune receptor J region-encoding gene segment.

In some embodiments, a′ is the same as a (described above for synthetic template oligonucleotides). In other embodiments, b′ is the same as b (described above for synthetic template oligonucleotides).

Thus, in certain embodiments and as also discussed elsewhere herein, the present synthetic template composition may be used in amplification reactions with amplification primers that are designed to amplify all rearranged adaptive immune receptor encoding gene sequences, including those that are not expressed. In certain other embodiments, the template composition and amplification primers may be designed so as not to yield amplification products of rearranged genes that are not expressed (e.g., pseudogenes, orphons). It will therefore be appreciated that in certain embodiments only a subset of rearranged adaptive immune receptor encoding genes may desirably be amplified, such that suitable amplification primer subsets may be designed and employed to amplify only those rearranged V-J sequences that are of interest. In these and related embodiments, correspondingly, a synthetic template composition comprising only a subset of interest of rearranged V-J rearranged sequences may be used, so long as the synthetic template composition comprises at least one synthetic template oligonucleotide to which each oligonucleotide amplification primer in an amplification primer set can anneal. The actual number of synthetic template oligonucleotides in the template composition may thus vary considerably among the contemplated embodiments, as a function of the amplification primer set that is to be used.

For example, in certain related embodiments, in the template composition, the plurality of synthetic template oligonucleotides comprise sequences found in SEQ ID NOs: 787-1644.

Primers for Use with Synthetic Template Oligonucleotides

The polynucleotide V in general formula (I) (or its complement) includes sequences to which members of oligonucleotide primer sets specific for TCR or BCR genes can specifically anneal. Primer sets that are capable of amplifying rearranged DNA encoding a plurality of TCR or BCR are described, for example, in U.S. Ser. No. 13/217,126; U.S. Ser. No. 12/794,507; PCT/US2011/026373; or PCT/US2011/049012; or the like; or as described therein may be designed to include oligonucleotide sequences that can specifically hybridize to each unique V gene and to each J gene in a particular TCR or BCR gene locus (e.g., TCR α, β, γ or δ, or IgH μ, γ, δ, α or ε, or IgL κ or λ).

For example, by way of illustration and not limitation, an oligonucleotide primer of an oligonucleotide primer amplification set that is capable of amplifying rearranged DNA encoding one or a plurality of TCR or BCR may typically include a nucleotide sequence of 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 or 40 contiguous nucleotides, or more, and may specifically anneal to a complementary sequence of 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 or 40 contiguous nucleotides of a V or a J polynucleotide as provided herein. In certain embodiments the primers may comprise at least 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 or 30 nucleotides, and in certain embodiment the primers may comprise sequences of no more than 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 or 40 contiguous nucleotides. Primers and primer annealing sites of other lengths are also expressly contemplated, as disclosed herein.

The polynucleotide J in general formula (I) (or its complement) includes sequences to which members of oligonucleotide primer sets specific for TCR or BCR genes can specifically anneal. Primer sets that are capable of amplifying rearranged DNA encoding a plurality of TCR or BCR are described, for example, in U.S. Ser. No. 13/217,126; U.S. Ser. No. 12/794,507; PCT/US2011/026373; or PCT/US2011/049012; or the like; or as described therein may be designed to include oligonucleotide sequences that can specifically hybridize to each unique V gene and to each unique J gene in a particular TCR or BCR gene locus (e.g., TCR α, β, γ or δ, or IgH μ, γ, δ, α or ε, or IgL κ or λ).

These V-segment and J-segment oligonucleotide primers can comprise universal adaptor sequences at their 5′-ends for sequencing the resulting amplicons, as described above and in U.S. Ser. No. 13/217,126; U.S. Ser. No. 12/794,507; PCT/US2011/026373; or PCT/US2011/049012. FIG. 1B illustrates primers that hybridize to specific regions of the V-segment and J-segment sequences and also include universal adaptor sequences.

In certain embodiments, oligonucleotide primer sets for amplification may be provided in substantially equimolar amounts. As also described herein, according to certain other embodiments, the concentration of one or more primers in a primer set may be adjusted deliberately so that certain primers are not present in equimolar amounts or in substantially equimolar amounts.

Adaptors

The herein described template oligonucleotides of general formula (I) also may in certain embodiments comprise first (U1) (102) and second (U2) (112) universal adaptor oligonucleotide sequences, or may lack either or both of U1 (102) and U2 (112). U1 (102) thus may comprise either nothing or an oligonucleotide having a sequence that is selected from (i) a first universal adaptor oligonucleotide sequence, and (ii) a first sequencing platform-specific oligonucleotide sequence that is linked to and positioned 5′ to a first universal adaptor oligonucleotide sequence, and U2 (112) may comprise either nothing or an oligonucleotide having a sequence that is selected from (i) a second universal adaptor oligonucleotide sequence, and (ii) a second sequencing platform-specific oligonucleotide sequence that is linked to and positioned 5′ to a second universal adaptor oligonucleotide sequence.

U1 (102) and/or U2 (112) may, for example, comprise universal adaptor oligonucleotide sequences and/or sequencing platform-specific oligonucleotide sequences that are specific to a single-molecule sequencing technology being employed, for example the HiSeq™ or GeneAnalyzer™-2 (GA-2) systems (Illumina, Inc., San Diego, Calif.) or another suitable sequencing suite of instrumentation, reagents and software. Inclusion of such platform-specific adaptor sequences permits direct quantitative sequencing of the presently described template composition, which comprises a plurality of different template oligonucleotides of general formula (I), using a nucleotide sequencing methodology such as the HiSeq™ or GA2 or equivalent. This feature therefore advantageously permits qualitative and quantitative characterization of the template composition.

In particular, the ability to sequence all components of the template composition directly allows for verification that each template oligonucleotide in the plurality of template oligonucleotides is present in a substantially equimolar amount. For example, a set of the presently described template oligonucleotides may be generated that have universal adaptor sequences at both ends, so that the adaptor sequences can be used to further incorporate sequencing platform-specific oligonucleotides at each end of each template.

Without wishing to be bound by theory, platform-specific oligonucleotides may be added onto the ends of such modified templates using 5′ (5′-platform sequence-universal adaptor-1 sequence-3′) and 3′ (5′-platform sequence-universal adaptor-2 sequence-3′) oligonucleotides in as little as two cycles of denaturation, annealing and extension, so that the relative representation in the template composition of each of the component template oligonucleotides is not quantitatively altered. Unique identifier sequences (e.g., barcode sequences B comprising unique V and B oligonucleotide sequences that are associated with and thus identify, respectively, individual V and J regions, as described herein) are placed adjacent to the adaptor sequences, thus permitting quantitative sequencing in short sequence reads, in order to characterize the template population by the criterion of the relative amount of each unique template sequence that is present.

Where such direct quantitative sequencing indicates that one or more particular oligonucleotides may be over- or underrepresented in a preparation of the template composition, adjustment of the template composition can be made accordingly to obtain a template composition in which all oligonucleotides are present in substantially equimolar amounts. The template composition in which all oligonucleotides are present in substantially equimolar amounts may then be used as a calibration standard for amplification primer sets, such as in the presently disclosed methods for determining and correcting non-uniform amplification potential among members of a primer set.

When primers are tailed with the universal+Illumina adaptors and sequenced with Illumina adaptors (see FIG. 1), these templates behave in the same fashion as typical synthetic templates. When amplified using VF and JR multiplex PCR primers and sequenced with JR primers, these molecules produce a sequencing read with the following structure (5′ to 3′): (1) J gene sequence (about 15 base pairs), (2) a 9 base pair synthetic template internal marker (IM), (3) a 16 base pair V-J barcode (BC), (4) a second 9 base pair synthetic template internal marker (IM), and (5) a V gene (about 15 base pairs).

In addition to adaptor sequences described in SEQ ID NOs: 765-786, other oligonucleotide sequences that may be used as universal adaptor sequences will be known to those familiar with the art in view of the present disclosure, including selection of adaptor oligonucleotide sequences that are distinct from sequences found in other portions of the herein described templates.

Barcodes

As described herein, certain embodiments contemplate designing the template oligonucleotide sequences to contain short signature sequences that permit unambiguous identification of the template sequence, and hence of at least one primer responsible for amplifying that template, without having to sequence the entire amplification product. In the herein described synthetic template oligonucleotides of general formula (I), B1, B2, B3, and B4 are each independently either nothing or each comprises an oligonucleotide B that comprises an oligonucleotide barcode sequence of 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900 or 1000 or more contiguous nucleotides (including all integer values therebetween), wherein in each of the plurality of template oligonucleotide sequences B comprises a unique oligonucleotide sequence that uniquely identifies, as a paired combination, (i) the unique V oligonucleotide sequence of the template oligonucleotide and (ii) the unique J oligonucleotide sequence of the template oligonucleotide.

Thus, for instance, synthetic template oligonucleotides having barcode identifier sequences may permit relatively short amplification product sequence reads, such as barcode sequence reads of no more than 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100, 90, 80, 70, 60, 55, 50, 45, 40, 35, 30, 25, 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4 or fewer nucleotides, followed by matching this barcode sequence information to the associated V and J sequences that are incorporated into the template having the barcode as part of the template design. By this approach, a large number of amplification products can be simultaneously partially sequenced by high throughput parallel sequencing, to identify primers that are responsible for amplification bias in a complex primer set.

Exemplary barcodes may comprise a first barcode oligonucleotide of 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 or 16 nucleotides that uniquely identifies each V polynucleotide in the template and a second barcode oligonucleotide of 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 or 16 nucleotides that uniquely identifies each J polynucleotide in the template, to provide barcodes of, respectively, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31 or 32 nucleotides in length, but these and related embodiments are not intended to be so limited. Barcode oligonucleotides may comprise oligonucleotide sequences of any length, so long as a minimum barcode length is obtained that precludes occurrence of a given barcode sequence in two or more template oligonucleotides having otherwise distinct sequences (e.g., V and J sequences).

Thus, the minimum barcode length, to avoid such redundancy amongst the barcodes that are used to uniquely identify different V-J sequence pairings, is X nucleotides, where 4X is greater than the number of distinct template species that are to be differentiated on the basis of having non-identical sequences. For example, for the set of 858 template oligonucleotides set forth in SEQ ID NOs: 1888-3003, the minimum barcode length would be five nucleotides, which would permit a theoretical total of 1024 (i.e., greater than 871) different possible pentanucleotide sequences. In practice, barcode oligonucleotide sequence read lengths may be limited only by the sequence read-length limits of the nucleotide sequencing instrument to be employed. For certain embodiments, different barcode oligonucleotides that will distinguish individual species of template oligonucleotides should have at least two nucleotide mismatches (e.g., a minimum hamming distance of 2) when aligned to maximize the number of nucleotides that match at particular positions in the barcode oligonucleotide sequences.

In preferred embodiments, for each distinct template oligonucleotide species having a unique sequence within the template composition of general formula (I), B1, B2, B3, and B4 will be identical.

The skilled artisan will be familiar with the design, synthesis, and incorporation into a larger oligonucleotide or polynucleotide construct, of oligonucleotide barcode sequences of, for instance, at least 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 30, 35, 40, 45, 50, 55, 60, 70, 80, 90, 100, 200, 300, 300, 500 or more contiguous nucleotides, including all integer values therebetween. For non-limiting examples of the design and implementation of oligonucleotide barcode sequence identification strategies, see, e.g., de Carcer et al., 2011 Adv. Env. Microbiol. 77:6310; Parameswaran et al., 2007 Nucl. Ac. Res. 35(19):330; Roh et al., 2010 Trends Biotechnol. 28:291.

Typically, barcodes are placed in templates at locations where they are not found naturally, i.e., barcodes comprise nucleotide sequences that are distinct from any naturally occurring oligonucleotide sequences that may be found in the vicinity of the sequences adjacent to which the barcodes are situated (e.g., V and/or J sequences). Such barcode sequences may be included, according to certain embodiments described herein, as elements B1, B2 and/or B3 of the presently disclosed template oligonucleotide of general formula (I). Accordingly, certain of the herein described template oligonucleotides of general formula (I) may also in certain embodiments comprise one, two or all three of barcodes B1, B2 and B3, while in certain other embodiments some or all of these barcodes may be absent. In certain embodiments all barcode sequences will have identical or similar GC content (e.g., differing in GC content by no more than 20%, or by no more than 19, 18, 17, 16, 15, 14, 13, 12, 11 or 10%).

In the template compositions according to certain herein disclosed embodiments the barcode-containing element B (e.g., B1, B2, B3, and/or B4) comprises the oligonucleotide sequence that uniquely identifies a single paired V-J combination. Optionally and in certain embodiments the barcode-containing element B may also include a random nucleotide, or a random polynucleotide sequence of at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 45, 50, 55, 60, 70, 80, 90, 100, 200, 300, 300, 500 or more contiguous nucleotides, situated upstream and/or downstream of the specific barcode sequence that uniquely identifies each specific paired V-J combination. When present both upstream and downstream of the specific barcode sequence, the random nucleotide or random polynucleotide sequence are independent of one another, that is, they may but need not comprise the same nucleotide or the same polynucleotide sequence.

Randomers

In some embodiments, the synthetic template oligonucleotide comprises a randomly generated oligonucleotide sequence, or a “randomer” sequence. The randomer sequence is generally situated between the V and J sequences, but can be located elsewhere along the synthetic template oligonucleotide. In an embodiment, the randomer sequence only occurs once in the synthetic template. N comprises a random oligonucleotide sequence of 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, or more than 30 contiguous nucleotides.

The number of possible nucleotide sequences of length X is 4X, thus a random nucleotide segment of even a short length may encode many possible unique nucleotide sequences. For example, a randomer sequence of 12 base pairs could encode any one of 16,777,216 unique nucleotide sequences. The randomer sequence ensures that any two synthetic template oligonucleotides have a probability of about 1 in 17 million of containing the same randomer sequence. Thus, tens or hundreds of thousands of synthetic template oligonucleotides can be included in the PCR reaction with minimal to no overlap in randomer sequences between two distinct synthetic template oligonucleotides.

Randomer sequences allow each synthetic template oligonucleotide to be quantitated exactly. Upon amplification of a pool of synthetic template oligonucleotides, each unique random nucleotide sequence observed in the sequencing output represents a single molecule of input material. Thus, the input number of synthetic template oligonucleotides added to the amplification reaction can be determined by counting the number of unique random nucleotide sequences. Furthermore, the input number of synthetic template oligonucleotides associated with a particular barcode (and thus associated with a particular paired combination of a V oligonucleotide sequence and J oligonucleotide sequence) can be determined by counting the number of unique random nucleotide sequences associated with a particular barcode. Examples of synthetic templates comprising randomers can be found, for example, in SEQ ID NOs: 3004-3159.

Restriction Enzyme Sites

According to certain embodiments disclosed herein, the template oligonucleotide can also comprise a restriction endonuclease (RE) recognition site that is situated between the V and J sequences and does not occur elsewhere in the template oligonucleotide sequence. The RE recognition site may optionally be adjacent to a barcode site that identifies the V region sequence. The RE site may be included for any of a number of purposes, including without limitation as a structural feature that may be exploited to destroy templates selectively by contacting them with the appropriate restriction enzyme. It may be desirable to degrade the present template oligonucleotides selectively by contacting them with a suitable RE, for example, to remove template oligonucleotides from other compositions into which they may have been deliberately or accidentally introduced. Alternatively, the RE site may be usefully exploited in the course of sequencing template oligonucleotides in the template composition, and/or as a positional sequence marker in a template oligonucleotide sequence regardless of whether or not it is cleaved with a restriction enzyme. An exemplary RE site is the oligonucleotide motif GTCGAC, which is recognized by the restriction enzyme Sal I. A large number of additional restriction enzymes and their respective RE recognition site sequences are known in the art and are available commercially (e.g., New England Biolabs, Beverly, Mass.). These include, for example, EcoRI (GAATTC) and SphI (GCATGC). Those familiar with the art will appreciate that any of a variety of such RE recognition sites may be incorporated into particular embodiments of the presently disclosed template oligonucleotides.

Control Synthetic Template Compositions Useful for Quantifying a Relative Representation of Adaptive Immune Cells in a Biological Sample

Control synthetic template oligonucleotides can be designed to quantify a number of input molecules in a biological sample. These control synthetic template oligonucleotides are similar to the synthetic template oligonucleotides described above, but do not contain a V oligonucleotide sequence or a J oligonucleotide sequence. When referring to synthetic templates, often the V and J region-containing oligonucleotides are referred to as a “first” set of synthetic templates while control synthetic templates are often referred to as a “second” set of synthetic templates. Instead, a control synthetic template composition comprises a plurality of template oligonucleotides of general formula (II):

5′-U1-B1-X1-B2-N-X2-B3-U2-3′  (II).

The segments U1, B1, B2, N, B3, and U2 are the same as described above. In an embodiment, X1 and X2 are either nothing or each comprise a polynucleotide comprising at least 10, 20, 30, or 40, and not more than 1000, 900, or 800 contiguous nucleotides of a DNA sequence. In some embodiments, the DNA sequence is of a genomic control gene (also referred to as an “internal control gene”), or the complement thereof. As used herein “genomic control gene” or “internal control gene” is any gene that is found in all cells (including both adaptive immune cells and cells that are not adaptive immune cells), such as a housekeeping gene like RNase P, PSMB2, RAB7A, UBC, VCP, REEF5, or EMC7.

Synthetic template oligonucleotides of formula (I) are used to determine a total number of input adaptive immune receptor molecules (and thus adaptive immune cells) in a biological sample. As explained below, control synthetic template oligonucleotides of formula (II) can be used to determine the total number of all input genomes in a biological sample, the biological sample including adaptive immune cells and cells that are not adaptive immune cells.

In some embodiments, a control synthetic template composition comprises one of the sequences found in SEQ ID NOs: 3160-3166 and 3241-3152. SEQ ID NOs: 3167-3194 show exemplary sequencing primers for control synthetic template compositions containing various control gene segments, SEQ ID NOs: 3195-3222 are exemplary primer sequences for adaptor sequences of the control synthetic template compositions, and SEQ ID NOs: 3223-3236 are exemplary primer sequences specific for the control synthetic template compositions. FIGS. 1A and 1B illustrate one example of a control synthetic template oligonucleotide, according to an embodiment of the invention.

In certain embodiments it is advantageous for the control synthetic control templates to be of similar length to synthetic templates containing TCR and/or Ig V and J or C segments. Furthermore, it is also advantageous in many embodiments for the synthetic templates (both control templates and those containing biological TCR or Ig sequences) to be of similar length to the amplification product of the TCR/Ig loci and the genomic control region from the input sample. In some embodiments, the length of the synthetic templates and corresponding amplicons from biological material are between about 100 and about 300 nucleotides (for example, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, or 300 nucleotides).

Methods of Use

I. Methods for Quantifying Tumor Infiltrating Lymphocytes without Randomers

a. Determining the Number of Input Synthetic Template Oligonucleotides in a Sample and an Amplification Factor Using a Limiting Dilution of Synthetic Template Oligonucleotides

In some embodiments, methods of the invention comprise determining the number of input synthetic template oligonucleotides in a sample and determining the amplification factor for each unique synthetic template.

The methods include steps for determining a number of synthetic template oligonucleotides added to a starting sample for use in PCR. The number of input synthetic template oligonucleotides can be estimated by using a limiting dilution of synthetic template oligonucleotides in a multiplex PCR assay. This number of input synthetic template oligonucleotides into a PCR assay and the number of output sequencing reads produced from the PCR assay can then be used to calculate an amplification ratio.

A limiting dilution is achieved when the amount of DNA in a sample is diluted to the point where only a very small subset of synthetic template oligonucleotides is present in the dilution. For example, in a pool of 1000 unique synthetic template oligonucleotides, the limiting dilution can include only 100 of the 1000 unique synthetic template oligonucleotides. Most of the unique synthetic templates would be absent in the limiting dilution. For example, the limiting dilution can include only 100 unique types of synthetic template oligonucleotide and only 1 copy of each unique synthetic template oligonucleotide. Thus, a portion of unique synthetic template oligonucleotides are added as a single copy or only a small number of copies, and the rest of the synthetic template oligonucleotides in the pool are added at zero copies (i.e., absent). In certain embodiments, the limiting dilution of the unique synthetic template oligonucleotides includes one molecule of each detectable, unique synthetic template oligonucleotide. In other embodiments, the limiting dilution can include two molecules of one or more of the detectable, unique synthetic template oligonucleotides. Thus, the limiting dilution includes a very low concentration of unique synthetic template oligonucleotides.

The limiting dilution of synthetic template oligonucleotides is amplified as part of a multiplex PCR, and the number of unique types of synthetic template oligonucleotide amplicons (having a unique barcode sequence, for example) is calculated.

Simplex PCR allows for amplification of each unique synthetic template oligonucleotide using one pair of PCR primers for all synthetic templates in the complete pool of synthetic template oligonucleotides. Simplex PCR can be performed on the synthetic template oligonucleotides by using universal primers that include the adaptor sequences and hybridize to the universal primer sequences (UA (102) and UB (112), as shown in FIG. 1B). Then, the resulting library of synthetic template oligonucleotide amplicons can be individually sequenced using the adaptor sequences on each amplicon on a sequencer, such as an Illumina sequencer. This process allows the direct measurement of the frequency of each synthetic template oligonucleotide in the complex pool.

In certain embodiments, an in silico simulation is used to analyze the relationship between the number of unique synthetic template oligonucleotide amplicons sequenced from the limiting dilution used in a multiplex PCR reaction and the estimated total input number of synthetic template oligonucleotides added to said multiplex PCR reaction. FIG. 2 provides an in silico simulation of the relationship between the number of unique types of synthetic template oligonucleotides observed (e.g., sequenced from the sample) and the number of synthetic template molecules sampled (e.g., number of synthetic template oligonucleotides in the starting sample). For example, if 400 unique types of synthetic template oligonucleotides are sequenced and observed from the sample, it can be determined that the starting sample included approximately 500 synthetic template oligonucleotide molecules. Accordingly, the total number of input synthetic template oligonucleotide can be determined from the number of unique synthetic template oligonucleotides observed.

A portion of this pool of synthetic template oligonucleotides can then be added into a multiplex PCR reaction comprising biological rearranged TCR or IG nucleic acid molecules obtained from lymphocytes in a given sample. The determined number of added (“spiked in”) synthetic template oligonucleotides and the calculated amplification ratio can be used to determine a total number of lymphocytes in the sample.

As described in detail herein, subsequent to the characterization of the synthetic template oligonucleotide pool, a limiting dilution of this pool can be added to a biological sample to determine the number of B or T cells present in said biological sample. An amplification factor is determined based on the number of synthetic template oligonucleotides in a starting sample of synthetic template oligonucleotides that has been added to a biological sample.

The amplification factor is calculated by comparing the number of total sequencing reads for synthetic template oligonucleotides observed from a sample with the total number of input synthetic template oligonucleotides in the sample. The amplification factor can then be used to determine the number of total lymphocytes (T cells or B cells) in a biological sample. This amplification factor can be assumed to apply to biological templates (e.g., rearranged TCR or IG nucleic acid molecules) that have been amplified with the same V-segment and J-segment-specific primers used to amplify synthetic template oligonucleotide molecules.

In an embodiment, the amplification factor (ratio) of the number of sequencing reads of synthetic template oligonucleotide amplicons to the number of total input synthetic template oligonucleotide molecules is compared to the number of total sequencing reads of biological molecule amplicons in order to calculate the starting number of input biological molecules. Thus, calculating the number of synthetic template oligonucleotide molecules at the start of the PCR assay can then be used in calculations of the relative representation of adaptive immune cells in the sample, as described in detail below.

b. Methods for Determining the Absolute Representation of Adaptive Immune Cells in a Sample

Methods are provided for determining the absolute representation of rearranged adaptive immune receptor encoding sequences in a sample.

Methods of the invention include extracting biological nucleic acid molecules (e.g., rearranged TCR or IG DNA molecules) from a biological sample comprising adaptive immune cells and cells that are not adaptive immune cells. The biological nucleic acid molecules in the sample are “spiked” with a known amount of synthetic template oligonucleotides (e.g., as described above in Section I. a. and determined by limiting dilution). The synthetic template oligonucleotides comprise the same combinations of V-segment and J-segment oligonucleotide sequences as the biological nucleic acid molecule templates.

In certain embodiments, the method for quantifying the absolute number of rearranged DNA molecules encoding a plurality of adaptive immune receptors in a biological sample of a subject, comprises the following steps:

I. Amplifying, in a multiplex PCR assay, a subset of synthetic template oligonucleotide molecules obtained from a pool of synthetic template oligonucleotides, the subset of synthetic template oligonucleotide molecules diluted such that only a single copy or a small number of copies of a portion of unique synthetic template oligonucleotides is present. The amplified synthetic template oligonucleotides are sequenced, and the number of unique synthetic template oligonucleotides based on unique barcode sequences is determined. The number of total sequencing reads from the synthetic template oligonucleotides is also determined from the sequencing output. Next, the results of an in silico simulation based on previous characterization of the synthetic template oligonucleotide pool (by simplex PCR) is referenced to determine from the number of unique synthetic template oligonucleotide sequences, the total input number of synthetic template oligonucleotide molecules (e.g., based on the relationship shown in FIG. 2). An amplification factor is determined from the ratio of the total output of sequencing reads from the sample and the estimated total number of input synthetic template oligonucleotides. This amplification factor can be used to estimate the total number of biological rearranged molecules, and thus, the total number of lymphoid cells, in a given sample. This can be done by adding (“spiking in”) a small portion of the pool of dilute synthetic template oligonucleotides to the multiplex PCR.

II. Amplifying nucleic acid molecules obtained from a given sample, in a multiplex PCR, using an oligonucleotide amplification primer set comprising V-segment and J-segment primers as described herein that are capable of amplifying substantially all V-segment and J-segment combinations of rearranged adaptive immune receptors, the sample comprising i) rearranged biological TCR or Ig adaptive immune receptor nucleic acid molecules, each comprising a V region and a J region, and ii) a portion of “spiked in” synthetic template oligonucleotides as described above having a known input amount, thereby generating amplicons comprising a plurality of uniquely rearranged TCR or Ig adaptive immune receptor amplicons and a plurality of synthetic template amplicons.

III. Quantitatively sequencing the plurality of uniquely rearranged TCR or Ig adaptive immune receptor amplicons and a plurality of synthetic template amplicons generated in (I) to determine the total number of rearranged TCR or Ig adaptive immune receptor amplicons observed by sequencing (herein referred to as Ai) and the total number of synthetic template amplicons observed by sequencing (herein referred to as Aii). The sequencing information includes the number of output sequencing products from the plurality of rearranged TCR or Ig adaptive immune receptor amplicons (Ai) and the number of output sequencing products from the synthetic template amplicons (Aii).

IV. Determining an absolute representation of adaptive immune cells in the sample based on the quantitative sequencing information determined from step II.

To determine the absolute representation of adaptive immune cells, an amplification factor is first calculated. The amplification factor is the ratio of the number of output sequencing products from the synthetic template amplicons (Aii) with the known number of input synthetic template oligonucleotides (referred to herein as Aiii). The number of input synthetic template oligonucleotides can be determined based on the in silico simulation performed in (I) to determine the relationship between the number of unique synthetic template oligonucleotide amplicons and the total input number of synthetic template oligonucleotides. It is assumed that the amplification factor of a particular primer set for a synthetic template oligonucleotide is the same amplification factor for the biological template.

Amplification factor=Aii/Aiii=number of output sequencing products from the synthetic template amplicons/known number of input synthetic template oligonucleotides.

In calculating this amplification factor, it is assumed that the ratio of the number of output sequencing reads per molecule of input is the same for a synthetic template oligonucleotide molecule and a biological rearranged TCR or Ig adaptive immune receptor nucleic acid molecule.

After calculating the amplification factor, the total number of rearranged TCR or Ig adaptive immune receptor molecules in the sample, and accordingly, the total number of lymphocyte cells, can be determined.

In an embodiment, the number of biological rearranged nucleic acid molecules encoding adaptive immune receptors is determined by the following:

Number of rearranged nucleic acid molecules encoding adaptive immune receptors=Ai/(Aii/Aiii)=(Number of output sequencing products determined from the plurality of rearranged TCR or Ig adaptive immune receptor amplicons)/(Amplification factor)

The total number of rearranged nucleic acid molecules encoding adaptive immune receptors is equal to the total number of adaptive immune cells (e.g., T cells or B cells) in the sample. Accordingly, the total number of adaptive immune cells in the sample can be determined.

c. Determining the Relative Representation of Adaptive Immune Cells in a Complex Mixture of Cells

Methods of the invention include determining a relative representation of adaptive immune cells in a complex mixture of cells that include adaptive immune cells and cells that are not adaptive immune cells. In some embodiments, the total number of adaptive immune cells is determined as described in Section I. b. and then used to calculate the relative representation of adaptive immune cells in the total sample of cells.

The total number of rearranged nucleic acid molecules encoding adaptive immune receptors (or total number of adaptive immune cells) is used to determine the relative representation of adaptive immune cells in the complex mixture. In one embodiment, the total mass of DNA in the sample is used to quantify the total number of genomes (adaptive immune cells and non-adaptive immune cells) in the complex mixture. Assuming that each cell has approximately 6.5 picograms of DNA and given a known total mass of input DNA to the PCR assay, the total number of total adaptive immune cells and non-adaptive immune cells in the sample is quantified by dividing the total known mass of input DNA by 6.5 picograms. This results in the relative representation of adaptive immune cells in the complex mixture of cells that include adaptive immune cells and cells that are not adaptive immune cells.

In other words, the relative representation of adaptive immune cells=total number of rearranged nucleic acid molecules encoding adaptive immune receptors/(total mass of DNA representing adaptive immune cells and non-adaptive immune cells).

Various other calculations as known to those of skill in the art can be used to determine the relative representation of adaptive immune cells in a complex mixture.

II. Methods for Quantifying Tumor Infiltrating Lymphocytes in a Sample Using Randomers and Control Genes

a. Determining the Number of Input Synthetic Template Oligonucleotides in a Sample and an Amplification Factor

In some embodiments, methods of the invention include determining a number of synthetic template oligonucleotides added to an input sample for use in PCR. The number of input synthetic template oligonucleotides can be estimated by amplifying a pool of synthetic template oligonucleotides that include randomers in a multiplex PCR assay, and counting the number of unique randomers observed in the nucleotide sequencing output. Since each synthetic template oligonucleotide in the input sample has a unique randomer sequence, each unique randomer sequence represents a single molecule, and the number of unique randomers in the nucleotide sequencing output represents the number of input synthetic template oligonucleotides.

In an embodiment, the input number of synthetic template oligonucleotides associated with a particular barcode (and thus associated with a particular paired combination of a V oligonucleotide sequence and J oligonucleotide sequence) can also be determined by counting the number of unique random DNA sequences associated with a particular barcode. The quantified number of input synthetic template oligonucleotides and the total number of output sequencing reads produced from the PCR assay can be used to calculate an amplification factor.

The amplification factor is determined based on the number of synthetic template oligonucleotides in a starting sample of synthetic template oligonucleotides that has been added to a biological sample. The amplification factor is calculated by comparing the number of total sequencing reads for synthetic template oligonucleotides observed from a sample with the total number of input synthetic template oligonucleotides in the sample, and can be used to determine the number of total lymphocytes (T cells or B cells) in a biological sample. This amplification factor can be assumed to apply to biological templates (e.g., rearranged TCR or IG nucleic acid molecules) that have been amplified with the same V-segment and J-segment-specific primers used to amplify synthetic template oligonucleotide molecules. In one embodiment, the amplification factor can also be defined as being based on the number of synthetic template oligonucleotides associated with a particular paired combination of a V oligonucleotide sequence and J oligonucleotide sequence in a biological sample. Thus, the amplification factor is calculated by comparing the number of total sequencing reads for synthetic template oligonucleotides associated with the particular paired combination, with the total number of input synthetic template oligonucleotides associated with the particular paired combination in the sample. This amplification factor can be used to determine the number of total lymphocytes (T cells or B cells) carrying the particular paired combination in a biological sample.

In an embodiment, the amplification factor (ratio) of the number of sequencing reads of synthetic template oligonucleotide amplicons to the number of total input synthetic template oligonucleotide molecules is compared to the number of total sequencing reads of biological molecule amplicons in order to calculate the starting number of input biological molecules. The number of synthetic template oligonucleotide molecules at the start of the PCR assay can then be used in calculations of the relative representation of adaptive immune cells in the sample, as described in detail below.

b. Methods for Determining the Absolute Representation of Adaptive Immune Cells in a Sample

Methods are provided for determining the absolute representation of rearranged adaptive immune receptor encoding sequences in a sample.

Methods of the invention include extracting biological nucleic acid molecules (e.g., rearranged TCR or IG DNA molecules) from a biological sample comprising adaptive immune cells and cells that are not adaptive immune cells. The biological nucleic acid molecules in the sample are “spiked” with synthetic template oligonucleotides. The synthetic template oligonucleotides comprise the same combinations of V-segment and J-segment oligonucleotide sequences as the biological nucleic acid molecule templates.

In certain embodiments, the method for quantifying the absolute number of rearranged DNA molecules encoding a plurality of adaptive immune receptors in a biological sample of a subject, comprises the following steps:

I. Amplifying nucleic acid molecules obtained from a given sample, in a multiplex PCR using an oligonucleotide amplification primer set comprising V-segment and J-segment primers as described herein capable of amplifying substantially all V-segment and J-segment combinations of rearranged adaptive immune receptors, the sample comprising i) rearranged TCR or IG adaptive immune receptor nucleic acid molecules, each comprising a V region and a J region, and ii) synthetic template oligonucleotides as described above, thereby generating amplicons comprising a plurality of uniquely rearranged TCR or IG adaptive immune receptor amplicons and a plurality of synthetic template amplicons.

II. Quantitatively sequencing the plurality of uniquely rearranged TCR or IG adaptive immune receptor amplicons and a plurality of synthetic template amplicons generated in (I) to determine the total number of rearranged TCR or IG adaptive immune receptor amplicons observed by sequencing (herein referred to as Ai) and the total number of synthetic template amplicons observed by sequencing (herein referred to as Aii). The sequencing information includes the number of output sequencing products from the plurality of rearranged TCR or Ig adaptive immune receptor amplicons (Ai) and the number of output sequencing products from the synthetic template amplicons (Aii).

III. Determining an absolute representation of adaptive immune cells in the sample based on the quantitative sequencing information determined from step II.

To determine the absolute representation of adaptive immune cells, an amplification factor is first calculated. The amplification factor is the ratio of the number of output sequencing products from the synthetic template amplicons (Aii) with the number of input synthetic template oligonucleotides (referred to herein as Aiii), calculated by counting the number of output synthetic template oligonucleotides having unique randomer sequences. It is assumed that the amplification factor of a particular primer set for a synthetic template oligonucleotide is the same amplification factor for the biological template.

Amplification factor=Aii/Aiii=number of output sequencing products from the synthetic template amplicons/known number of input synthetic template oligonucleotides.

In calculating this amplification factor, it is assumed that the ratio of the number of output sequencing reads per molecule of input is the same for a synthetic template oligonucleotide molecule and a biological rearranged TCR or Ig adaptive immune receptor nucleic acid molecule.

After calculating the amplification factor, the total number of rearranged TCR or Ig adaptive immune receptor molecules in the sample, and accordingly, the total number of lymphocyte cells, can be determined.

In an embodiment, the number of biological rearranged nucleic acid molecules encoding adaptive immune receptors is determined by the following:

Number of rearranged nucleic acid molecules encoding adaptive immune receptors=Ai/(Aii/Aiii)=(Number of output sequencing products determined from the plurality of rearranged TCR or Ig adaptive immune receptor amplicons)/(Amplification factor)

The total number of rearranged nucleic acid molecules encoding adaptive immune receptors is equal to the total number of adaptive immune cells (e.g., T cells or B cells) in the sample. Accordingly, the total number of adaptive immune cells in the sample can be determined.

c. Determining the Relative Representation of Adaptive Immune Cells in a Complex Mixture of Cells

Methods of the invention include determining a relative representation of adaptive immune cells in a complex mixture of cells that include adaptive immune cells and cells that are not adaptive immune cells. In some embodiments, the total number of adaptive immune cells is determined as described in the section above and then used to calculate the relative representation of adaptive immune cells in the total sample of cells.

In certain embodiments, the method for quantifying the relative representation of adaptive immune cells in a complex mixture of cells is similar to the method described above for quantifying the absolute number of rearranged DNA molecules. However, the method for quantifying the relative representation includes the use of control synthetic template oligonucleotides and control gene segment primers to determine the total number of input genomes in a biological sample. The method comprises the following steps:

I. Amplifying nucleic acid molecules obtained from a given sample, in a multiplex PCR using an oligonucleotide amplification primer set comprising V-segment and J-segment primers as described herein capable of amplifying substantially all V-segment and J-segment combinations of rearranged adaptive immune receptors, and a control gene segment primer set comprising a plurality of primers capable of amplifying at least a portion of a control gene that is found in adaptive immune cells and cells that are not adaptive immune cells. The given sample comprises i) rearranged TCR or IG adaptive immune receptor nucleic acid molecules, each comprising a V region and a J region, ii) synthetic template oligonucleotides as described above, iii) control gene segment molecules, and iv) control synthetic template oligonucleotides as described above, thereby generating amplicons comprising a plurality of uniquely rearranged TCR or IG adaptive immune receptor amplicons, a plurality of control gene segment amplicons, a plurality of synthetic template amplicons, and a plurality of control synthetic template amplicons.

II. Quantitatively sequencing the plurality of uniquely rearranged TCR or Ig adaptive immune receptor amplicons, the plurality of control gene segment amplicons, the plurality of synthetic template amplicons, and the plurality of control synthetic template amplicons generated in (I) to determine the total number of rearranged TCR or Ig adaptive immune receptor amplicons observed by sequencing (herein referred to as Ai), the total number of synthetic template amplicons observed by sequencing (herein referred to as Aii), the total number of control gene segment amplicons observed by sequencing (herein referred to as Bi), and the total number of control synthetic template amplicons observed by sequencing (herein referred to as Bii). The sequencing information includes the number of output sequencing products from the plurality of rearranged TCR or Ig adaptive immune receptor amplicons (Ai), the number of output sequencing products from the synthetic template amplicons (Aii), the number of output sequencing products from the control gene segment amplicons (Bi), and the number of output sequencing products from the control synthetic template amplicons (Bii).

III. Determining an absolute representation of adaptive immune cells in the sample based on the quantitative sequencing information determined from step II. This determination of the absolute representation of adaptive immune cells is described in detail above.

IV. Determining an absolute representation of total cells (adaptive immune cells and cells that are not adaptive immune cells) in the sample based on the quantitative sequencing information determined from step II.

To determine the absolute representation of total cells, a control amplification factor is first calculated. The control amplification factor is the ratio of the number of output sequencing products from the control synthetic template amplicons (Bii) with the number of input control synthetic template oligonucleotides (referred to herein as Biii), calculated by counting the number of output control synthetic template oligonucleotides having unique randomer sequences. It is assumed that the control amplification factor for the control synthetic template oligonucleotide is the same amplification factor for the biological template.

Control amplification factor=Bii/Biii=number of output sequencing products from the control synthetic template amplicons/number of input control synthetic template oligonucleotides.

In calculating this control amplification factor, it is assumed that the ratio of the number of output sequencing reads per molecule of input is the same for a control synthetic template oligonucleotide molecule and a biological control gene segment molecule.

After calculating the control amplification factor, the total number of genomes (or cells) in the sample can be determined.

In an embodiment, the number of total input genomes is determined by the following:

Number of total input genomes=Bi/(Bii/Biii)=(Number of output sequencing products determined from the control gene segment amplicons)/(Control amplification factor)

The total number of input genomes is the total number of cells (adaptive immune cells and cells that are not adaptive immune cells) in the sample. Accordingly, the total number of cells in the sample can be determined.

V. Determining the relative representation of adaptive immune cells in the complex mixture of cells based on the absolute representation of adaptive immune cells in the sample and the absolute representation of total cells in the sample. The total number of adaptive immune cells is compared with the total number of cells. In an embodiment, the relative representation of adaptive immune cells is determined by dividing the total number of adaptive immune cells in the sample by the total number of cells in the sample.

In another embodiment, the total mass of DNA in the sample is used to quantify the total number of adaptive immune cells and non-adaptive immune cells in the complex mixture. Assuming that each cell has approximately 6.5 picograms of DNA and given a known total mass of input DNA to the PCR assay, the total number of total adaptive immune cells and non-adaptive immune cells in the sample is quantified by dividing the total known mass of input DNA by 6.5 picograms. This results in the relative representation of adaptive immune cells in the complex mixture of cells that include adaptive immune cells and cells that are not adaptive immune cells.

In other words, the relative representation of adaptive immune cells=total number of rearranged nucleic acid molecules encoding adaptive immune receptors/(total mass of DNA representing adaptive immune cells and non-adaptive immune cells).

Various other calculations as known to those of skill in the art can be used to determine the relative representation of adaptive immune cells in a complex mixture.

d. Methods for Determining a Ratio of T Cells or B Cells in a Sample Relative to the Total Number of Input Genomes Using Synthetic Templates and One or More Control Genes (Genomic Control Regions)

Methods of the invention include steps for determining a ratio of T cells or B cells in a sample relative to the total number of input genomes contained in said sample comprising:

A) amplifying by multiplex PCR and sequencing:

    • i) rearranged CDR3 oligonucleotide sequences from T cell receptor (TCR) loci from T cells or Immunoglobulin (Ig) loci from B cells in said sample to obtain a total number of output biological sequences, each oligonucleotide sequence comprising a V segment and a J segment;
    • ii) a first set of synthetic templates representing substantially all possible V segment and J segment combinations and each comprising one TCR or Ig V segment and one TCR or Ig J or C segment and a unique barcode which identifies said synthetic template as synthetic, and wherein each synthetic template comprises a unique combination of a V segment and J or C segment;

B) determining an amplification factor for each synthetic template comprising a unique combination of a V segment and a J or C segment, wherein said amplification factor is represented by a total number of first synthetic templates observed from step A(ii) divided by a total input number of unique first synthetic templates input in step A(ii);

C) determining the total number of T cells or B cells in the sample by dividing the total number of output biological sequences observed in step A(i) by the amplification factor from step B;

D) amplifying by multiplex PCR and sequencing:

i) one or more genomic control regions from DNA obtained from said sample to obtain a total number of output biological sequences for each genomic control region; and

ii) a second set of synthetic templates comprising the sequence of one or more of said genomic control sequences, a unique barcode and a stretch of random nucleic acids, wherein each synthetic template is represented only once;

E) determining an amplification factor for each of said genomic control region by dividing the total number of second synthetic templates amplified and sequenced in step D(ii) by the total input number of unique second synthetic templates amplified and sequenced in step D(ii);

F) determining the total number of input genomes by dividing the total number of output biological sequences for each genomic control region from step D(i) by the corresponding amplification factor for that genomic control region from Step E; and

G) determining the ratio of T cells or B cells contained in the sample relative to the number of total genomes in the sample by dividing the total number of T cells or B cells obtained in step C by the total number of input genomes obtained in step F.

In some embodiments, the method comprises amplifying by multiplex PCR and sequencing two, three, four, five or more genomic control regions in step D(i). In certain embodiments, five genomic control regions are amplified and sequenced in step D(i).

In other embodiments, the total number of input genomes is calculated in step F by taking an average using each of the five amplification factors determined for each of said five genomic control regions. In another embodiment, the highest and lowest calculated number of input genomes is discarded prior to taking said average.

Examples of genomic control regions are PPIA, PSMB2, RAB7A, UBC, VCP, REEP5, and EMC7 or any other gene that has a known or predictable copy number (See SEQ ID NOs: 3160-3166 and 3241-3252 for a more comprehensive, albeit non-exhaustive list of genomic control regions.

In some embodiment, the total number of synthetic templates in said first set of synthetic templates subject to amplification in step A(ii) is determined using a limiting dilution of said synthetic templates each comprising a unique TCR or Ig V and J or C region such that each unique synthetic template is found in a single copy.

In other embodiments, the total number of synthetic templates in said first set of synthetic templates subject to amplification in step A(ii) is determined by counting the number of unique synthetic templates based on the unique random nucleotides contained in each synthetic template.

III. Methods for Diagnosing, Preventing, or Treating Disease in Patients Based on Determining Relative Representation of Adaptive Immune Cells in a Patient's Sample

According to certain embodiments, methods are provided for determining a course of treatment for a patient in need thereof, comprising quantifying the relative representation of tumor-infiltrating lymphocytes or lymphocytes infiltrating a somatic tissue that is the target of an autoimmune reaction, using the methods described herein. In this regard, the patient in need thereof may be a cancer patient or a patient having an autoimmune disease. In certain embodiments, a patient may have a cancer including, but not limited to, colorectal, hepatocellular, gallbladder, pancreatic, esophageal, lung, breast, prostate, skin (e.g., melanoma), head and neck, renal cell carcinoma, ovarian, endometrial, cervical, bladder and urothelial cancer. In certain other embodiments, a patient may have an organ transplant, such as a liver transplant, a lung transplant, a kidney transplant, a heart transplant, a spleen transplant, a pancreas transplant, a skin transplant/graft, an intestine transplant, and a thymus transplant.

Autoimmune diseases include, but are not limited to, arthritis (including rheumatoid arthritis, reactive arthritis), systemic lupus erythematosus (SLE), psoriasis, inflammatory bowel disease (IBD) (including ulcerative colitis and Crohn's disease), encephalomyelitis, uveitis, myasthenia gravis, multiple sclerosis, insulin dependent diabetes, Addison's disease, celiac disease, chronic fatigue syndrome, autoimmune hepatitis, autoimmune alopecia, ankylosing spondylitis, fibromyalgia, pemphigus vulgaris, Sjogren's syndrome, Kawasaki's Disease, hyperthyroidism/Graves disease, hypothyroidism/Hashimoto's disease, endometriosis, scleroderma, pernicious anemia, Goodpasture syndrome, Guillain-Barré syndrome, Wegener's disease, glomerulonephritis, aplastic anemia (including multiply transfused aplastic anemia patients), paroxysmal nocturnal hemoglobinuria, idiopathic thrombocytopenic purpura, autoimmune hemolytic anemia, Evan's syndrome, Factor VIII inhibitor syndrome, systemic vasculitis, dermatomyositis, polymyositis and rheumatic fever, autoimmune lymphoproliferative syndrome (ALPS), autoimmune bullous pemphigoid, Parkinson's disease, sarcoidosis, vitiligo, primary biliary cirrhosis, and autoimmune myocarditis.

The methods described herein may be used to enumerate the relative presence of tumor-infiltrating lymphocytes, or of lymphocytes infiltrating a somatic tissue that is the target of an autoimmune reaction, based on quantification of the relative representation of DNA from such adaptive immune cells in DNA extracted from a biological sample, comprising a mixture of cell types, that has been obtained from such a tumor or tissue. Such methods are useful for determining cancer or autoimmune disease prognosis and diagnosis, for assessing effects of a therapeutic treatment (e.g., assessing drug efficacy and/or dose-response relationships), and for identifying therapeutic courses for cancer treatment, for treatment of autoimmune diseases, or for treatment of transplant rejection, and may find other related uses.

To assess a therapeutic treatment, for example, certain embodiments contemplate a method in which is assessed an effect of the therapeutic treatment on the relative representation of adaptive immune cells in at least one tissue in a subject to whom the treatment has been administered. By way of illustration and not limitation, according to certain such embodiments a treatment that alters (e.g., increases or decreases in a statistically significant manner) the relative representation of adaptive immune cells in a tissue or tissues may confer certain benefits on the subject. For instance, certain cancer immunotherapies are designed to enhance the number of tumor infiltrating lymphocytes (TIL). It has been shown that the presence of CD3+ TIL in ovarian tumors is strongly correlated with patient outcome (see, e.g., Hwang et al., 2011 Gynecol. Oncol., 124(2):192). Further data clarified that in addition to TIL presence, the characteristics of the TIL populations were also significant: CD8+ TILs and clonal TILs were associated with longer Disease Free Survival (DFS), and infiltrating regulatory T cells were associated with shorter DFS (see, Stumpf et al., 2009 Br. J. Cancer 101:1513-21). These studies indicated that TIL may be an independent prognostic factor (see, Clarke et al., 2009 Mod. Pathol. 22:393-402). Thus, quantification of the relative representation of adaptive immune cell DNA as described herein, for purposes of detecting possible increases in TIL in tumor tissue samples obtained at one or a plurality of time points before treatment, during the course of treatment and/or following treatment may provide highly useful information with respect to determining efficacy of the treatment, and therefrom developing a prognosis for the subject.

As another example, certain autoimmune disease-directed immunotherapies are designed to reduce the number of tissue infiltrating lymphocytes in one or more afflicted tissues such as tissues or organs that may be targets of clinically inappropriate autoimmune attack, such that quantification of the relative representation of adaptive immune cell DNA as described herein, for purposes of detecting possible decreases in adaptive immune cells in tissue samples obtained at one or a plurality of time points before treatment, during the course of treatment and/or following treatment may provide highly useful information with respect to determining efficacy of the treatment, and therefrom developing a prognosis for the subject.

As a further example, certain transplant rejection-directed immunotherapies are designed to reduce the number of tissue infiltrating lymphocytes in transplanted organs, such that quantification of the relative representation of adaptive immune cell DNA as described herein, for purposes of detecting possible decreases in adaptive immune cells in tissue samples from transplanted organs obtained at one or a plurality of time points before treatment, during the course of treatment and/or following treatment may provide highly useful information with respect to determining efficacy of the treatment, and therefrom developing a prognosis for the subject.

In these and related embodiments, the herein described methods for quantifying the relative representation of adaptive immune cell DNA may be practiced using test biological samples obtained from a subject at one or a plurality of time points prior to administering the therapeutic treatment to the subject, and at one or a plurality of time points after administering the therapeutic treatment to the subject. The samples may be obtained from the same or from different tissues, which may vary as a function of the particular condition of the subject. For example, by way of illustration and not limitation, in the case of an inoperable tumor the test biological samples that are obtained from the subject before and after treatment may be from the same tissue, whereas in the case of a tumor that is partially removed surgically, or that occurs at multiple sites in the subject, the test biological samples may be obtained from different tissues or from different tissue sites before and after the therapeutic treatment is administered.

Also contemplated herein are embodiments in which any of the herein described methods may further comprise determination of the relative structural diversity of adaptive immune receptors (e.g., the sequence diversity among products of productively rearranged TCR and/or immunoglobulin genes) in the adaptive immune cell component of the mixture of cells that is present in the test biological sample. In certain such embodiments, the present qPCR methodologies using the herein described rearranged adaptive immune receptor encoding specific oligonucleotide primer sets permit ready identification of the particular primer combinations that generate the production of amplified rearranged DNA molecules. Accordingly, for example, these embodiments permit determination of the relative degree of clonality of an adaptive immune cell population that is present as part of a mixed cell population in a test biological sample, which may have prognostic value.

For instance, in a solid tumor sample in which TILs are detected by quantifying the relative representation of adaptive immune cell DNA in DNA extracted from the sample as described herein, the present methods contemplate determination of whether only one or a few (e.g., no more than 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10) combinations of a particular V-segment oligonucleotide primer and a particular J-segment oligonucleotide primer are predominantly (e.g., generating at least 80, 85, 90, 95, 97 or 99 percent of amplification products) responsible for the PCR production of amplified rearranged adaptive immune cell DNA molecules. Such an observation of one or a few predominant adaptive immune receptor gene-encoding amplification product would, according to non-limiting theory, indicate a low degree of TIL heterogeneity. Conversely, determination of a high degree of heterogeneity in adaptive immune receptor structural diversity by characterization of TIL DNA would indicate that a predominant TIL clone is not present.

Accordingly, described herein are methods for measuring the number of adaptive immune cells (e.g. T cells) in a complex mixture of cells. The present methods have particular utility in quantifying tumor-infiltrating lymphocytes or lymphocytes infiltrating somatic tissue that is the target of an autoimmune response. Existing methods for T and B cell quantification rely upon the physical separation of such cells from the mixture. However, in many cases, T and B cells cannot be separated from the initial sample, such as formalin-fixed or frozen tissue samples. Furthermore, prior methods for adaptive immune cell quantification (e.g., flow immunocytofluorimetry, fluorescence activated cell sorting (FACS), immunohistochemistry (IHC)) rely on the expression of T cell- or B cell-specific proteins, such as cell surface receptors. Since immune cells express varying amounts of these lineage specific receptors, quantifying the number of cells from such a highly variable measure requires costly standardization, specialized equipment and highly trained staff. The presently disclosed methods are, by contrast, platform-independent and can be performed on any PCR instrument and high-throughput sequencing instrument, and the reagents can be synthesized and provided in kit form. The presently disclosed methods are also highly sensitive and can be applied in high throughput settings not previously attainable. As described herein, quantification of adaptive immune cells may be achieved by a simple preparation of DNA from a complex mixture of cells, in concert with quantification of the relative proportion of adaptive immune cells present by amplification of the rearranged adaptive immune cell CDR3-encoding genes.

In certain embodiments, the invention includes methods for comparing adaptive immune cell DNA quantities with total cell DNA (e.g., from adaptive immune cells plus non-adaptive immune cells in the cell mixture). Methods also include optionally comparing other relevant parameters before, during or after administration to a control subject of control compositions that can be, for example, negative controls that have been previously demonstrated to have undergone no statistically significant alteration of physiological state, such as sham injection, saline, DMSO or other vehicle or buffer control, inactive enantiomers, scrambled peptides or nucleotides, etc., and/or before, during or after administration of positive controls that have been previously demonstrated to cause a statistically significant alteration of physiological state, such as an FDA-approved therapeutic compound.

The practice of certain embodiments of the present invention will employ, unless indicated specifically to the contrary, conventional methods in microbiology, molecular biology, biochemistry, molecular genetics, cell biology, virology and immunology techniques that are within the skill of the art, and reference to several of which is made below for the purpose of illustration. Such techniques are explained fully in the literature. See, e.g., Sambrook, et al., Molecular Cloning: A Laboratory Manual (3rd Edition, 2001); Sambrook, et al., Molecular Cloning: A Laboratory Manual (2nd Edition, 1989); Maniatis et al., Molecular Cloning: A Laboratory Manual (1982); Ausubel et al., Current Protocols in Molecular Biology (John Wiley and Sons, updated July 2008); Short Protocols in Molecular Biology: A Compendium of Methods from Current Protocols in Molecular Biology, Greene Pub. Associates and Wiley-Interscience; Glover, DNA Cloning: A Practical Approach, vol. I & II (IRL Press, Oxford Univ. Press USA, 1985); Current Protocols in Immunology (Edited by: John E. Coligan, Ada M. Kruisbeek, David H. Margulies, Ethan M. Shevach, Warren Strober 2001 John Wiley & Sons, NY, N.Y.); Real-Time PCR: Current Technology and Applications, Edited by Julie Logan, Kirstin Edwards and Nick Saunders, 2009, Caister Academic Press, Norfolk, UK; Anand, Techniques for the Analysis of Complex Genomes, (Academic Press, New York, 1992); Guthrie and Fink, Guide to Yeast Genetics and Molecular Biology (Academic Press, New York, 1991); Oligonucleotide Synthesis (N. Gait, Ed., 1984); Nucleic Acid Hybridization (B. Hames & S. Higgins, Eds., 1985); Transcription and Translation (B. Hames & S. Higgins, Eds., 1984); Animal Cell Culture (R. Freshney, Ed., 1986); Perbal, A Practical Guide to Molecular Cloning (1984); Next-Generation Genome Sequencing (Janitz, 2008 Wiley-VCH); PCR Protocols (Methods in Molecular Biology) (Park, Ed., 3rd Edition, 2010 Humana Press); Immobilized Cells And Enzymes (IRL Press, 1986); the treatise, Methods In Enzymology (Academic Press, Inc., N.Y.); Gene Transfer Vectors For Mammalian Cells (J. H. Miller and M. P. Calos eds., 1987, Cold Spring Harbor Laboratory); Harlow and Lane, Antibodies, (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1998); Immunochemical Methods In Cell And Molecular Biology (Mayer and Walker, eds., Academic Press, London, 1987); Handbook Of Experimental Immunology, Volumes I-IV (D. M. Weir and C C Blackwell, eds., 1986); Riott, Essential Immunology, 6th Edition, (Blackwell Scientific Publications, Oxford, 1988); Embryonic Stem Cells: Methods and Protocols (Methods in Molecular Biology) (Kurstad Turksen, Ed., 2002); Embryonic Stem Cell Protocols: Volume I: Isolation and Characterization (Methods in Molecular Biology) (Kurstad Turksen, Ed., 2006); Embryonic Stem Cell Protocols: Volume II: Differentiation Models (Methods in Molecular Biology) (Kurstad Turksen, Ed., 2006); Human Embryonic Stem Cell Protocols (Methods in Molecular Biology) (Kursad Turksen Ed., 2006); Mesenchymal Stem Cells: Methods and Protocols (Methods in Molecular Biology) (Darwin J. Prockop, Donald G Phinney, and Bruce A. Bunnell Eds., 2008); Hematopoietic Stem Cell Protocols (Methods in Molecular Medicine) (Christopher A. Klug, and Craig T. Jordan Eds., 2001); Hematopoietic Stem Cell Protocols (Methods in Molecular Biology) (Kevin D. Bunting Ed., 2008) Neural Stem Cells: Methods and Protocols (Methods in Molecular Biology) (Leslie P. Weiner Ed., 2008).

Unless specific definitions are provided, the nomenclature utilized in connection with, and the laboratory procedures and techniques of, molecular biology, analytical chemistry, synthetic organic chemistry, and medicinal and pharmaceutical chemistry described herein are those well known and commonly used in the art. Standard techniques may be used for recombinant technology, molecular biological, microbiological, chemical syntheses, chemical analyses, pharmaceutical preparation, formulation, and delivery, and treatment of patients.

Unless the context requires otherwise, throughout the present specification and claims, the word “comprise” and variations thereof, such as, “comprises” and “comprising” are to be construed in an open, inclusive sense, that is, as “including, but not limited to”. By “consisting of” is meant including, and typically limited to, whatever follows the phrase “consisting of.” By “consisting essentially of” is meant including any elements listed after the phrase, and limited to other elements that do not interfere with or contribute to the activity or action specified in the disclosure for the listed elements. Thus, the phrase “consisting essentially of” indicates that the listed elements are required or mandatory, but that no other elements are required and may or may not be present depending upon whether or not they affect the activity or action of the listed elements.

In this specification and the appended claims, the singular forms “a,”“an” and “the” include plural references unless the content clearly dictates otherwise. As used herein, in particular embodiments, the terms “about” or “approximately” when preceding a numerical value indicates the value plus or minus a range of 5%, 6%, 7%, 8% or 9%. In other embodiments, the terms “about” or “approximately” when preceding a numerical value indicates the value plus or minus a range of 10%, 11%, 12%, 13% or 14%. In yet other embodiments, the terms “about” or “approximately” when preceding a numerical value indicates the value plus or minus a range of 15%, 16%, 17%, 18%, 19% or 20%.

Reference throughout this specification to “one embodiment” or “an embodiment” or “an aspect” means that a particular feature, structure or characteristic described in connection with the embodiment is included in at least one embodiment of the present invention. Thus, the appearances of the phrases “in one embodiment” or “in an embodiment” in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.

EXAMPLES

Below are examples of specific embodiments for carrying out the present invention. The examples are offered for illustrative purposes only, and are not intended to limit the scope of the present invention in any way. Efforts have been made to ensure accuracy with respect to numbers used (e.g., amounts, temperatures, etc.), but some experimental error and deviation should, of course, be allowed for.

Example 1: Constructing Synthetic Templates and Estimating the Number of Input Synthetic Templates

Synthetic templates were designed and constructed as shown in FIGS. 1A and 1B. Each synthetic template included a 495 bp sequence comprising a universal primer sequence (UA) (102), a 16 bp template-specific barcode (BC) (104), a 300 bp adaptive immune receptor variable (V) region encoding gene sequence (V gene) (106), a 9 bp synthetic template internal marker (IM) (108), a repeat of the barcode (BC) (104), a repeat of the internal marker (IM) (108), a 100 bp adaptive immune receptor variable (J) region encoding gene sequence (J gene) (110), a third repeat of the barcode (BC) (104), and a reverse universal primer sequence (UB) (112). Synthetic templates can also be designed as described in Carlson, C. S. et al. Using synthetic templates to design an unbiased multiplex PCR assay. Nature Communications 4, 2680, doi: 10.1038/ncomms3680 (2013). Synthetic templates were constructed that included substantially all V-segment and J-segment combinations of rearranged adaptive immune receptors. Sequences of the synthetic templates are found in Table A.

The starting pool of synthetic templates was quantified using simplex PCR. Universal primers specific for the universal primer regions (102) and (112) and tailed with Illumina adaptor regions were used to amplify and sequence the synthetic templates. To estimate the number of sequencing reads of the synthetic template amplicons generated per input synthetic template, the number of input synthetic templates added to the PCR was estimated using a limiting dilution. A limiting dilution was achieved by diluting the amount of DNA in each sample to the point where only a very small amount of mixed synthetic template oligonucleotides was present in the dilution (e.g., a small subset of unique synthetic template oligonucleotides at a low copy per template). When a limiting dilution of synthetic template oligonucleotides was added to the PCR assay, the majority of unique synthetic template oligonucleotides was not detected by PCR in the sample, and the synthetic template oligonucleotides that were detected were likely to have been added as a single copy. Thus, the simplex PCR results accurately characterized the concentration of each unique synthetic template in the sample.

FIG. 2 is a graph showing the relationship between the number of unique synthetic templates in the sample and the total number of synthetic templates in the sample. This relationship was analyzed using an in silico simulation of a random sampling from the pool of synthetic templates. The mean and standard deviation of the number of unique synthetic templates observed (y-axis) are presented as a function of the total number of molecules sampled from the synthetic template oligonucleotide pool (x-axis). The graph demonstrates that the detected number of unique synthetic template oligonucleotides provides information regarding the total number of molecules in the pool. Aliquots of this pool of synthetic templates can be spiked in to subsequent multiplex PCRs (in the Examples below, for instance) and used to determine a number of input molecules in a particular sample.

Example 2: Validating Method of Estimating the Number of Input Synthetic Templates

To validate the method described in Example 1 of estimating the number of input synthetic templates added to the PCR, bulk fibroblast gDNA and gDNA from sorted (CD19+) B cells were used to construct mixtures of 300 ng total gDNA, containing various combinations of B cell DNA (from 0.07 ng to 300 ng) and fibroblast DNA. Since each sample contained a known proportion of input B cell DNA, the number of rearranged IgH loci in each sample was also known. This number was compared to the number of rearranged IgH input synthetic templates estimated using the method described in Example 1. Examples of rearranged IgH input synthetic template sequences are found in SEQ ID NOs: 1645-3003.

This validation experiment was replicated four times using four separate PCRs and sequencing reactions. The graph in FIG. 3 shows that the four PCRs exhibited replicable results, and that the Example 1 method of estimating the number of input synthetic templates was successful, since the estimated numbers were in agreement with the known numbers of rearranged IgH loci.

Example 3: Determining a Relative Representation of T Cells in a Mixture of Cells

To determine the relative representation of tumor-infiltrating lymphocytes (TILs) in four tissue samples taken from high-grade serous ovarian carcinomas, TCRB ddPCR was performed (as described in U.S. application Ser. No. 13/656,265; and Robins, H. S. et al. Digital genomic quantification of tumor-infiltrating lymphocytes. Science Translational Medicine 5, 214ra169, doi:10.1126/scitranslmed.3007247 (2013), hereby incorporated by reference). Furthermore, synthetic templates were used to separately determine the relative representation of TILs. First, a sample was prepared that included both gDNA extracted from the tumor tissue and synthetic templates as shown in FIG. 1 including substantially all V-segment and J-segment combinations of rearranged adaptive immune receptors (synthetic template sequences are found in SEQ ID NOs: 787-1644). Next, a multiplex PCR was performed on the sample using V-segment and J-segment primers to amplify nucleic acid molecules comprising i) rearranged TCR or Ig adaptive immune receptor nucleic acid molecules, each comprising a V region and a J region, and ii) synthetic template oligonucleotides having a known input amount, thereby generating amplicons comprising a plurality of rearranged TCR or Ig adaptive immune receptor amplicons and a plurality of synthetic template amplicons.

The amplicons were sequenced to determine the number of rearranged TCR or Ig adaptive immune receptor amplicons and the number of unique synthetic template amplicons. The amplification factor was determined by calculating the ratio of the number of output sequencing products from the synthetic template amplicons with the known number of input synthetic template oligonucleotides, determined using the estimation method of Example 1 and described herein.

Next, the absolute number of input rearranged TCR or Ig adaptive immune receptor molecules in the sample was determined by dividing the number of output sequencing products determined from the rearranged TCR or Ig adaptive immune receptor amplicons by the amplification factor. The absolute number of input rearranged TCR or Ig receptor molecules equates to the total number adaptive immune cells in the sample.

From there, the number of total adaptive immune cells and non-adaptive immune cells in the sample was determined by dividing a total known mass of input DNA by 6.5 picograms (assuming that each cell has approximately 6.5 picograms of DNA and given a known total mass of input DNA to the PCR assay). The relative representation of adaptive immune cells in the sample was calculated by dividing the number of unique adaptive immune cells by the total number of adaptive immune cells and non-adaptive immune cells in the sample.

FIG. 4 is a graph showing (1) the relative representation of T cells in the sample determined using ddPCR methods, as described in U.S. application Ser. No. 13/656,265, filed on Oct. 21, 2012, and International App. No. PCT/US2012/061193, filed on Oct. 21, 2012, each of which is incorporated by reference in its entirety, as compared with (2) the relative representation of T cells in the sample using synthetic templates and the methods described above. FIG. 4 shows that similar numbers are calculated using both methods. Therefore, synthetic templates can be used to accurately calculate the relative representation of adaptive immune cells in a sample containing adaptive immune cells and non-adaptive immune cells.

Example 4: Determining a Relative Representation of T Cells in a Mixture of Cells Using Genomic Control Regions

In this example, synthetic templates and genomic control genes were used to accurately calculate the relative representation of adaptive immune cells in a sample containing adaptive immune cells and non-adaptive immune cells.

Sample Source:

T cells were isolated from whole blood using standard cell biology techniques. DNA was extracted from the population of purified T cells. DNA was normalized, assuming 6.4 pg DNA/double stranded human genome such that approximately 5 genomes, 250 genomes, 1250 genomes, or 6250 genomes of T cell DNA were added to a standard TCRB PCR reaction.

Multiplex PCR Reaction:

TCRB Assay: Rearranged TCRB genes were amplified using a multiplex PCR. V segment and J segment primers were designed to amplify ˜110 bp rearranged fragments. Synthetic templates were added to each PCR reaction and were amplified with the same primers, and the synthetic templates included a barcode to differentiate them from biologic templates. The volume of DNA necessary to add 5, 250, 1250, and 6250 genomes were added to each PCR reaction.

A second PCR tailing reaction was performed using tailing primers comprising well-specific barcodes and Illumina sequencing adaptors. The PCR tailing reaction added well-specific barcodes and Illumina sequencing adaptors to each PCR product.

Genomic Control Assay:

In addition to the TCRB assay, five single copy autosomal loci were amplified using a multiplex PCR assay. Each single copy autosomal locus is present in every cell and serves as a genomic control. The genomic controls were used to count the number of genomes present in the sample. Primers were designed to amplify 110 bp fragments of each locus, which were the same size as the TCRB primers.

The multiplex PCR reaction included co-amplification of synthetic templates that include oligonucleotide sequences of each of the five autosomal genes. The synthetic templates included unique barcodes that identify the molecules as synthetic templates and a 6 bp random sequence. The same concentration of DNA for genomic controls was used as the TCRB genes, but at an eighth of the volume, such that less than 1 genome, 31, 156, 781 double stranded genomes were added to each PCR reaction. Well specific barcodes and illumina sequencing adaptors were added to each PCR product in a second tailing PCR assay, as described above.

Sequencing:

Samples were pooled, normalized, and loaded on an Illumina MiSEQ. Output sequence data was processed, and sequence reads of the synthetic templates were used to measure sequencing coverage. Sequencing coverage is an estimate of the number of sequencing clusters derived from a single molecule added to the PCR reaction.

Analysis:

The number of TCRB molecules in the sample was estimated using the methods described above. The number of genomes added to the TCRB assay was determined by estimating the number of genomes in the genomic control assay as previously described (Section III). The calculated number of genomes from the genomic control assay was scaled by 4 to account for 1) that there were 2 loci/genome and 2) the eight fold reduction of input (FIG. 6).

While the invention has been particularly shown and described with reference to a preferred embodiment and various alternate embodiments, it will be understood by persons skilled in the relevant art that various changes in form and details can be made therein without departing from the spirit and scope of the invention.

All references, issued patents and patent applications cited within the body of the instant specification are hereby incorporated by reference in their entirety, for all purposes.


SEQUENCE LISTING
The patent contains a lengthy “Sequence Listing” section. A copy of the “Sequence Listing” is available in electronic form from the USPTO web site (). An electronic copy of the “Sequence Listing” will also be available from the USPTO upon request and payment of the fee set forth in 37 CFR 1.19(b)(3).

<160> NUMBER OF SEQ ID NOS: 3252

<140> CURRENT APPLICATION NUMBER: US/15/304,631

<210> SEQ ID NO 1

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 1

gaatgccctg acagctctcg cttata 26

<210> SEQ ID NO 2

<211> LENGTH: 41

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 2

ctcagagaag tctgaaatat tcgatgatca attctcagtt g 41

<210> SEQ ID NO 3

<211> LENGTH: 34

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 3

ccaaatcgmt tctcacctaa atctccagac aaag 34

<210> SEQ ID NO 4

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 4

cacctgactc tccagacaaa gctcat 26

<210> SEQ ID NO 5

<211> LENGTH: 22

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 5

cctgaatgcc ccaacagctc tc 22

<210> SEQ ID NO 6

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 6

gattctcagg gcgccagttc tcta 24

<210> SEQ ID NO 7

<211> LENGTH: 31

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 7

cctaattgat tctcagctca ccacgtccat a 31

<210> SEQ ID NO 8

<211> LENGTH: 19

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 8

tcagggcgcc agttccatg 19

<210> SEQ ID NO 9

<211> LENGTH: 29

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 9

tcctagattc tcaggtctcc agttcccta 29

<210> SEQ ID NO 10

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 10

gaggaaactt ccctgatcga ttctcagc 28

<210> SEQ ID NO 11

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 11

caacttccct gatcgattct caggtca 27

<210> SEQ ID NO 12

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 12

aggaaacttc cctgatcaat tctcaggtca 30

<210> SEQ ID NO 13

<211> LENGTH: 29

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 13

ggaaacttcc ctcctagatt ttcaggtcg 29

<210> SEQ ID NO 14

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 14

ccccaatggc tacaatgtct ccagatt 27

<210> SEQ ID NO 15

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 15

ggagaggtcc ctgatggcta caa 23

<210> SEQ ID NO 16

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 16

tccctgatgg ttatagtgtc tccagagc 28

<210> SEQ ID NO 17

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 17

ggagaagtcc ccaatggcta caatgtc 27

<210> SEQ ID NO 18

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 18

aaaggagaag tcccgaatgg ctacaa 26

<210> SEQ ID NO 19

<211> LENGTH: 29

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 19

gttcccaatg gctacaatgt ctccagatc 29

<210> SEQ ID NO 20

<211> LENGTH: 32

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 20

gaagtcccca atggctacaa tgtctctaga tt 32

<210> SEQ ID NO 21

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 21

gagaagtccc cgatggctac aatgta 26

<210> SEQ ID NO 22

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 22

gtgatcggtt ctctgcacag aggt 24

<210> SEQ ID NO 23

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 23

cgcttctctg cagagaggac tgg 23

<210> SEQ ID NO 24

<211> LENGTH: 22

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 24

ggttctttgc agtcaggcct ga 22

<210> SEQ ID NO 25

<211> LENGTH: 22

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 25

cagtggtcgg ttctctgcag ag 22

<210> SEQ ID NO 26

<211> LENGTH: 29

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 26

gctcagtgat caattctcca cagagaggt 29

<210> SEQ ID NO 27

<211> LENGTH: 22

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 27

ttctctgcag agaggcctga gg 22

<210> SEQ ID NO 28

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 28

cccagtgatc gcttctttgc agaaa 25

<210> SEQ ID NO 29

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 29

ctgcagagag gcctaaggga tct 23

<210> SEQ ID NO 30

<211> LENGTH: 33

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 30

gaagggtaca atgtctctgg aaacaaactc aag 33

<210> SEQ ID NO 31

<211> LENGTH: 31

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 31

ggggtactgt gtttcttgaa acaagcttga g 31

<210> SEQ ID NO 32

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 32

cagttccctg acttgcactc tgaactaaac 30

<210> SEQ ID NO 33

<211> LENGTH: 32

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 33

actaacaaag gagaagtctc agatggctac ag 32

<210> SEQ ID NO 34

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 34

agataaagga gaagtccccg atggcta 27

<210> SEQ ID NO 35

<211> LENGTH: 35

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 35

gatactgaca aaggagaagt ctcagatggc tatag 35

<210> SEQ ID NO 36

<211> LENGTH: 29

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 36

ctaaggatcg attttctgca gagaggctc 29

<210> SEQ ID NO 37

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 37

ttgattctca gcacagatgc ctgatgt 27

<210> SEQ ID NO 38

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 38

attctcagct gagaggcctg atgg 24

<210> SEQ ID NO 39

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 39

ggatcgattc tcagctaaga tgcctaatgc 30

<210> SEQ ID NO 40

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 40

ctcagcagag atgcctgatg caacttta 28

<210> SEQ ID NO 41

<211> LENGTH: 29

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 41

ctgatcgatt ctcagctcaa cagttcagt 29

<210> SEQ ID NO 42

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 42

tagctgaaag gactggaggg acgtat 26

<210> SEQ ID NO 43

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 43

ccaggaggcc gaacacttct ttct 24

<210> SEQ ID NO 44

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 44

gctaagtgcc tcccaaattc accct 25

<210> SEQ ID NO 45

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 45

cacagctgaa agacctaacg gaacgt 26

<210> SEQ ID NO 46

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 46

ctgctgaatt tcccaaagag ggcc 24

<210> SEQ ID NO 47

<211> LENGTH: 20

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 47

agggtacagc gtctctcggg 20

<210> SEQ ID NO 48

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 48

gcctgacctt gtccactctg aca 23

<210> SEQ ID NO 49

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 49

atgagcgatt tttagcccaa tgctcca 27

<210> SEQ ID NO 50

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 50

tgaaggctac gtgtctgcca agag 24

<210> SEQ ID NO 51

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 51

ctcatctcaa tgccccaaga acgc 24

<210> SEQ ID NO 52

<211> LENGTH: 31

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 52

agatctctga tggatacagt gtctctcgac a 31

<210> SEQ ID NO 53

<211> LENGTH: 34

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 53

agatctttcc tctgagtcaa cagtctccag aata 34

<210> SEQ ID NO 54

<211> LENGTH: 35

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 54

cactgaaaaa ggagatatct ctgaggggta tcatg 35

<210> SEQ ID NO 55

<211> LENGTH: 31

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 55

gttcctgaag ggtacaaagt ctctcgaaaa g 31

<210> SEQ ID NO 56

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 56

ctgaggggta cagtgtctct agagaga 27

<210> SEQ ID NO 57

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 57

agccgcccaa acctaacatt ctcaa 25

<210> SEQ ID NO 58

<211> LENGTH: 18

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 58

cccaggaccg gcagttca 18

<210> SEQ ID NO 59

<211> LENGTH: 40

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 59

ttgattagag acatatccct attgaaaata tttcctggca 40

<210> SEQ ID NO 60

<211> LENGTH: 31

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer

<400> SEQUENCE: 60

agatgccctg agtcagcata gtcattctaa c 31

<210> SEQ ID NO 61

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 61

gggctggcaa gccacgtttg gtggaatgcc ctgacagctc tcgcttata 49

<210> SEQ ID NO 62

<211> LENGTH: 64

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 62

gggctggcaa gccacgtttg gtgctcagag aagtctgaaa tattcgatga tcaattctca 60

gttg 64

<210> SEQ ID NO 63

<211> LENGTH: 57

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 63

gggctggcaa gccacgtttg gtgccaaatc gmttctcacc taaatctcca gacaaag 57

<210> SEQ ID NO 64

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 64

gggctggcaa gccacgtttg gtgcacctga ctctccagac aaagctcat 49

<210> SEQ ID NO 65

<211> LENGTH: 45

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 65

gggctggcaa gccacgtttg gtgcctgaat gccccaacag ctctc 45

<210> SEQ ID NO 66

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 66

gggctggcaa gccacgtttg gtggattctc agggcgccag ttctcta 47

<210> SEQ ID NO 67

<211> LENGTH: 54

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 67

gggctggcaa gccacgtttg gtgcctaatt gattctcagc tcaccacgtc cata 54

<210> SEQ ID NO 68

<211> LENGTH: 42

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 68

gggctggcaa gccacgtttg gtgtcagggc gccagttcca tg 42

<210> SEQ ID NO 69

<211> LENGTH: 52

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 69

gggctggcaa gccacgtttg gtgtcctaga ttctcaggtc tccagttccc ta 52

<210> SEQ ID NO 70

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 70

gggctggcaa gccacgtttg gtggaggaaa cttccctgat cgattctcag c 51

<210> SEQ ID NO 71

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 71

gggctggcaa gccacgtttg gtgcaacttc cctgatcgat tctcaggtca 50

<210> SEQ ID NO 72

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 72

gggctggcaa gccacgtttg gtgaggaaac ttccctgatc aattctcagg tca 53

<210> SEQ ID NO 73

<211> LENGTH: 52

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 73

gggctggcaa gccacgtttg gtgggaaact tccctcctag attttcaggt cg 52

<210> SEQ ID NO 74

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 74

gggctggcaa gccacgtttg gtgccccaat ggctacaatg tctccagatt 50

<210> SEQ ID NO 75

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 75

gggctggcaa gccacgtttg gtgggagagg tccctgatgg ctacaa 46

<210> SEQ ID NO 76

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 76

gggctggcaa gccacgtttg gtgtccctga tggttatagt gtctccagag c 51

<210> SEQ ID NO 77

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 77

gggctggcaa gccacgtttg gtgggagaag tccccaatgg ctacaatgtc 50

<210> SEQ ID NO 78

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 78

gggctggcaa gccacgtttg gtgaaaggag aagtcccgaa tggctacaa 49

<210> SEQ ID NO 79

<211> LENGTH: 52

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 79

gggctggcaa gccacgtttg gtggttccca atggctacaa tgtctccaga tc 52

<210> SEQ ID NO 80

<211> LENGTH: 55

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 80

gggctggcaa gccacgtttg gtggaagtcc ccaatggcta caatgtctct agatt 55

<210> SEQ ID NO 81

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 81

gggctggcaa gccacgtttg gtggagaagt ccccgatggc tacaatgta 49

<210> SEQ ID NO 82

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 82

gggctggcaa gccacgtttg gtggtgatcg gttctctgca cagaggt 47

<210> SEQ ID NO 83

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 83

gggctggcaa gccacgtttg gtgcgcttct ctgcagagag gactgg 46

<210> SEQ ID NO 84

<211> LENGTH: 45

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 84

gggctggcaa gccacgtttg gtgggttctt tgcagtcagg cctga 45

<210> SEQ ID NO 85

<211> LENGTH: 45

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 85

gggctggcaa gccacgtttg gtgcagtggt cggttctctg cagag 45

<210> SEQ ID NO 86

<211> LENGTH: 52

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 86

gggctggcaa gccacgtttg gtggctcagt gatcaattct ccacagagag gt 52

<210> SEQ ID NO 87

<211> LENGTH: 45

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 87

gggctggcaa gccacgtttg gtgttctctg cagagaggcc tgagg 45

<210> SEQ ID NO 88

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 88

gggctggcaa gccacgtttg gtgcccagtg atcgcttctt tgcagaaa 48

<210> SEQ ID NO 89

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 89

gggctggcaa gccacgtttg gtgctgcaga gaggcctaag ggatct 46

<210> SEQ ID NO 90

<211> LENGTH: 56

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 90

gggctggcaa gccacgtttg gtggaagggt acaatgtctc tggaaacaaa ctcaag 56

<210> SEQ ID NO 91

<211> LENGTH: 54

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 91

gggctggcaa gccacgtttg gtgggggtac tgtgtttctt gaaacaagct tgag 54

<210> SEQ ID NO 92

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 92

gggctggcaa gccacgtttg gtgcagttcc ctgacttgca ctctgaacta aac 53

<210> SEQ ID NO 93

<211> LENGTH: 55

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 93

gggctggcaa gccacgtttg gtgactaaca aaggagaagt ctcagatggc tacag 55

<210> SEQ ID NO 94

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 94

gggctggcaa gccacgtttg gtgagataaa ggagaagtcc ccgatggcta 50

<210> SEQ ID NO 95

<211> LENGTH: 58

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 95

gggctggcaa gccacgtttg gtggatactg acaaaggaga agtctcagat ggctatag 58

<210> SEQ ID NO 96

<211> LENGTH: 52

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 96

gggctggcaa gccacgtttg gtgctaagga tcgattttct gcagagaggc tc 52

<210> SEQ ID NO 97

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 97

gggctggcaa gccacgtttg gtgttgattc tcagcacaga tgcctgatgt 50

<210> SEQ ID NO 98

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 98

gggctggcaa gccacgtttg gtgattctca gctgagaggc ctgatgg 47

<210> SEQ ID NO 99

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 99

gggctggcaa gccacgtttg gtgggatcga ttctcagcta agatgcctaa tgc 53

<210> SEQ ID NO 100

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 100

gggctggcaa gccacgtttg gtgctcagca gagatgcctg atgcaacttt a 51

<210> SEQ ID NO 101

<211> LENGTH: 52

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 101

gggctggcaa gccacgtttg gtgctgatcg attctcagct caacagttca gt 52

<210> SEQ ID NO 102

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 102

gggctggcaa gccacgtttg gtgtagctga aaggactgga gggacgtat 49

<210> SEQ ID NO 103

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 103

gggctggcaa gccacgtttg gtgccaggag gccgaacact tctttct 47

<210> SEQ ID NO 104

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 104

gggctggcaa gccacgtttg gtggctaagt gcctcccaaa ttcaccct 48

<210> SEQ ID NO 105

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 105

gggctggcaa gccacgtttg gtgcacagct gaaagaccta acggaacgt 49

<210> SEQ ID NO 106

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 106

gggctggcaa gccacgtttg gtgctgctga atttcccaaa gagggcc 47

<210> SEQ ID NO 107

<211> LENGTH: 43

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 107

gggctggcaa gccacgtttg gtgagggtac agcgtctctc ggg 43

<210> SEQ ID NO 108

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 108

gggctggcaa gccacgtttg gtggcctgac cttgtccact ctgaca 46

<210> SEQ ID NO 109

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 109

gggctggcaa gccacgtttg gtgatgagcg atttttagcc caatgctcca 50

<210> SEQ ID NO 110

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 110

gggctggcaa gccacgtttg gtgtgaaggc tacgtgtctg ccaagag 47

<210> SEQ ID NO 111

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 111

gggctggcaa gccacgtttg gtgctcatct caatgcccca agaacgc 47

<210> SEQ ID NO 112

<211> LENGTH: 54

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 112

gggctggcaa gccacgtttg gtgagatctc tgatggatac agtgtctctc gaca 54

<210> SEQ ID NO 113

<211> LENGTH: 57

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 113

gggctggcaa gccacgtttg gtgagatctt tcctctgagt caacagtctc cagaata 57

<210> SEQ ID NO 114

<211> LENGTH: 58

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 114

gggctggcaa gccacgtttg gtgcactgaa aaaggagata tctctgaggg gtatcatg 58

<210> SEQ ID NO 115

<211> LENGTH: 54

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 115

gggctggcaa gccacgtttg gtggttcctg aagggtacaa agtctctcga aaag 54

<210> SEQ ID NO 116

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 116

gggctggcaa gccacgtttg gtgctgaggg gtacagtgtc tctagagaga 50

<210> SEQ ID NO 117

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 117

gggctggcaa gccacgtttg gtgagccgcc caaacctaac attctcaa 48

<210> SEQ ID NO 118

<211> LENGTH: 41

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 118

gggctggcaa gccacgtttg gtgcccagga ccggcagttc a 41

<210> SEQ ID NO 119

<211> LENGTH: 63

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 119

gggctggcaa gccacgtttg gtgttgatta gagacatatc cctattgaaa atatttcctg 60

gca 63

<210> SEQ ID NO 120

<211> LENGTH: 54

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB V-Segment Forward Primer With Universal Adaptor Sequence

<400> SEQUENCE: 120

gggctggcaa gccacgtttg gtgagatgcc ctgagtcagc atagtcattc taac 54

<210> SEQ ID NO 121

<211> LENGTH: 29

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB J-segment Reverse Primer

<400> SEQUENCE: 121

gtcttaccta caactgtgag tctggtgcc 29

<210> SEQ ID NO 122

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB J-segment Reverse Primer

<400> SEQUENCE: 122

ccttacctac aacggttaac ctggtccc 28

<210> SEQ ID NO 123

<211> LENGTH: 31

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB J-segment Reverse Primer

<400> SEQUENCE: 123

cttactcacc tacaacagtg agccaacttc c 31

<210> SEQ ID NO 124

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB J-segment Reverse Primer

<400> SEQUENCE: 124

atacccaaga cagagagctg ggttcc 26

<210> SEQ ID NO 125

<211> LENGTH: 29

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB J-segment Reverse Primer

<400> SEQUENCE: 125

aacttaccta ggatggagag tcgagtccc 29

<210> SEQ ID NO 126

<211> LENGTH: 22

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB J-segment Reverse Primer

<400> SEQUENCE: 126

ctgtcacagt gagcctggtc cc 22

<210> SEQ ID NO 127

<211> LENGTH: 18

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB J-segment Reverse Primer

<400> SEQUENCE: 127

cacggtgagc cgtgtccc 18

<210> SEQ ID NO 128

<211> LENGTH: 22

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB J-segment Reverse Primer

<400> SEQUENCE: 128

ccagtacggt cagcctagag cc 22

<210> SEQ ID NO 129

<211> LENGTH: 18

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB J-segment Reverse Primer

<400> SEQUENCE: 129

cactgtcagc cgggtgcc 18

<210> SEQ ID NO 130

<211> LENGTH: 18

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB J-segment Reverse Primer

<400> SEQUENCE: 130

cactgagagc cgggtccc 18

<210> SEQ ID NO 131

<211> LENGTH: 17

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB J-segment Reverse Primer

<400> SEQUENCE: 131

accaggagcc gcgtgcc 17

<210> SEQ ID NO 132

<211> LENGTH: 18

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB J-segment Reverse Primer

<400> SEQUENCE: 132

cacggtcagc ctgctgcc 18

<210> SEQ ID NO 133

<211> LENGTH: 18

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB J-segment Reverse Primer

<400> SEQUENCE: 133

gaccgtgagc ctggtgcc 18

<210> SEQ ID NO 134

<211> LENGTH: 52

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB J-segment Reverse Primer With Universal Adaptor Sequence

<400> SEQUENCE: 134

ccgggagctg catgtgtcag agggtcttac ctacaactgt gagtctggtg cc 52

<210> SEQ ID NO 135

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB J-segment Reverse Primer With Universal Adaptor Sequence

<400> SEQUENCE: 135

ccgggagctg catgtgtcag aggccttacc tacaacggtt aacctggtcc c 51

<210> SEQ ID NO 136

<211> LENGTH: 54

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB J-segment Reverse Primer With Universal Adaptor Sequence

<400> SEQUENCE: 136

ccgggagctg catgtgtcag aggcttactc acctacaaca gtgagccaac ttcc 54

<210> SEQ ID NO 137

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB J-segment Reverse Primer With Universal Adaptor Sequence

<400> SEQUENCE: 137

ccgggagctg catgtgtcag aggataccca agacagagag ctgggttcc 49

<210> SEQ ID NO 138

<211> LENGTH: 52

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB J-segment Reverse Primer With Universal Adaptor Sequence

<400> SEQUENCE: 138

ccgggagctg catgtgtcag aggaacttac ctaggatgga gagtcgagtc cc 52

<210> SEQ ID NO 139

<211> LENGTH: 45

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB J-segment Reverse Primer With Universal Adaptor Sequence

<400> SEQUENCE: 139

ccgggagctg catgtgtcag aggctgtcac agtgagcctg gtccc 45

<210> SEQ ID NO 140

<211> LENGTH: 41

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB J-segment Reverse Primer With Universal Adaptor Sequence

<400> SEQUENCE: 140

ccgggagctg catgtgtcag aggcacggtg agccgtgtcc c 41

<210> SEQ ID NO 141

<211> LENGTH: 45

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB J-segment Reverse Primer With Universal Adaptor Sequence

<400> SEQUENCE: 141

ccgggagctg catgtgtcag aggccagtac ggtcagccta gagcc 45

<210> SEQ ID NO 142

<211> LENGTH: 41

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB J-segment Reverse Primer With Universal Adaptor Sequence

<400> SEQUENCE: 142

ccgggagctg catgtgtcag aggcactgtc agccgggtgc c 41

<210> SEQ ID NO 143

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB J-segment Reverse Primer With Universal Adaptor Sequence

<400> SEQUENCE: 143

gtcttaccta caactgtgag tctggtgccc actgagagcc gggtccc 47

<210> SEQ ID NO 144

<211> LENGTH: 40

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB J-segment Reverse Primer With Universal Adaptor Sequence

<400> SEQUENCE: 144

ccgggagctg catgtgtcag aggaccagga gccgcgtgcc 40

<210> SEQ ID NO 145

<211> LENGTH: 41

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB J-segment Reverse Primer With Universal Adaptor Sequence

<400> SEQUENCE: 145

ccgggagctg catgtgtcag aggcacggtc agcctgctgc c 41

<210> SEQ ID NO 146

<211> LENGTH: 41

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRB J-segment Reverse Primer With Universal Adaptor Sequence

<400> SEQUENCE: 146

ccgggagctg catgtgtcag agggaccgtg agcctggtgc c 41

<210> SEQ ID NO 147

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRG V-segment Forward Primer

<400> SEQUENCE: 147

ggaggggaag gccccacagc gtcttc 26

<210> SEQ ID NO 148

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRG V-segment Forward Primer

<400> SEQUENCE: 148

tgaagtcata cagttcctgg tgtccat 27

<210> SEQ ID NO 149

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRG V-segment Forward Primer

<400> SEQUENCE: 149

ccaaatcagg ctttggagca cctgatct 28

<210> SEQ ID NO 150

<211> LENGTH: 33

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRG V-segment Forward Primer

<400> SEQUENCE: 150

ccaaacaaag gcttagaata tttattacat gtc 33

<210> SEQ ID NO 151

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRG V-segment Forward Primer

<400> SEQUENCE: 151

ccaggtccct gaggcactcc accagct 27

<210> SEQ ID NO 152

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRG V-segment Forward Primer

<400> SEQUENCE: 152

ctgaatctaa attatgagcc atctgaca 28

<210> SEQ ID NO 153

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRG V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 153

gggctggcaa gccacgtttg gtgggagggg aaggccccac agcgtcttc 49

<210> SEQ ID NO 154

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRG V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 154

gggctggcaa gccacgtttg gtgtgaagtc atacagttcc tggtgtccat 50

<210> SEQ ID NO 155

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRG V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 155

gggctggcaa gccacgtttg gtgccaaatc aggctttgga gcacctgatc t 51

<210> SEQ ID NO 156

<211> LENGTH: 56

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRG V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 156

gggctggcaa gccacgtttg gtgccaaaca aaggcttaga atatttatta catgtc 56

<210> SEQ ID NO 157

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRG V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 157

gggctggcaa gccacgtttg gtgccaggtc cctgaggcac tccaccagct 50

<210> SEQ ID NO 158

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRG V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 158

gggctggcaa gccacgtttg gtgctgaatc taaattatga gccatctgac a 51

<210> SEQ ID NO 159

<211> LENGTH: 34

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRG J-segment Reverse Primer

<400> SEQUENCE: 159

gtgaagttac tatgagctta gtcccttcag caaa 34

<210> SEQ ID NO 160

<211> LENGTH: 33

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRG J-segment Reverse Primer

<400> SEQUENCE: 160

cgaagttact atgagcctag tcccttttgc aaa 33

<210> SEQ ID NO 161

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRG J-segment Reverse Primer

<400> SEQUENCE: 161

tgacaacaag tgttgttcca ctgccaaa 28

<210> SEQ ID NO 162

<211> LENGTH: 31

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRG J-segment Reverse Primer

<400> SEQUENCE: 162

ctgtaatgat aagctttgtt ccgggaccaa a 31

<210> SEQ ID NO 163

<211> LENGTH: 57

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRG J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 163

ccgggagctg catgtgtcag agggtgaagt tactatgagc ttagtccctt cagcaaa 57

<210> SEQ ID NO 164

<211> LENGTH: 56

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRG J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 164

ccgggagctg catgtgtcag aggcgaagtt actatgagcc tagtcccttt tgcaaa 56

<210> SEQ ID NO 165

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRG J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 165

ccgggagctg catgtgtcag aggtgacaac aagtgttgtt ccactgccaa a 51

<210> SEQ ID NO 166

<211> LENGTH: 54

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRG J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 166

ccgggagctg catgtgtcag aggctgtaat gataagcttt gttccgggac caaa 54

<210> SEQ ID NO 167

<211> LENGTH: 32

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 167

tcattcctta gtcgctctga tagttatggt ta 32

<210> SEQ ID NO 168

<211> LENGTH: 31

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 168

cattccttag tcggtctaaa gggtacagtt a 31

<210> SEQ ID NO 169

<211> LENGTH: 31

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 169

acaacatgac ctatgaacgg ttctcttcat c 31

<210> SEQ ID NO 170

<211> LENGTH: 31

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 170

ctgaatttaa caagagccaa acctccttcc a 31

<210> SEQ ID NO 171

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 171

ccgacagaaa gtccagcact ctgag 25

<210> SEQ ID NO 172

<211> LENGTH: 36

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 172

cactgttcta ttgaataaaa aggataaaca tctgtc 36

<210> SEQ ID NO 173

<211> LENGTH: 32

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 173

gtcacctttg ataccaccct taaacagagt tt 32

<210> SEQ ID NO 174

<211> LENGTH: 35

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 174

agactaaatg ctacattact gaagaatgga agcag 35

<210> SEQ ID NO 175

<211> LENGTH: 36

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 175

tgaggctgaa tttataaaga gtaaattctc ctttaa 36

<210> SEQ ID NO 176

<211> LENGTH: 32

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 176

gctgaattta agaagagtga aacctccttc ca 32

<210> SEQ ID NO 177

<211> LENGTH: 33

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 177

ggctgaattt aagaggagtc aatcttcctt caa 33

<210> SEQ ID NO 178

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 178

gacacttatc acttccccaa tcaatacccc 30

<210> SEQ ID NO 179

<211> LENGTH: 33

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 179

ggctgaattt aacaagagtc aaacttcctt cca 33

<210> SEQ ID NO 180

<211> LENGTH: 32

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 180

gctgaattta agaagagcga aacctccttc ta 32

<210> SEQ ID NO 181

<211> LENGTH: 31

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 181

ccatgtaccg taaagaaacc acttctttcc a 31

<210> SEQ ID NO 182

<211> LENGTH: 31

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 182

ccacataccg taaagaaacc acttctttcc a 31

<210> SEQ ID NO 183

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 183

tggatgcaga cacaaagcaa agctc 25

<210> SEQ ID NO 184

<211> LENGTH: 36

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 184

taaagaactg cttggaaaag aaaaatttta tagtgt 36

<210> SEQ ID NO 185

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 185

acagctcaat agagccagcc agtatatttc 30

<210> SEQ ID NO 186

<211> LENGTH: 29

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 186

cagctcaata aagccagcca gtatgtttc 29

<210> SEQ ID NO 187

<211> LENGTH: 32

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 187

gcacaggtcg ataaatccag caagtatatc tc 32

<210> SEQ ID NO 188

<211> LENGTH: 34

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 188

ctgttacatt gaacaagaca gccaaacatt tctc 34

<210> SEQ ID NO 189

<211> LENGTH: 36

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 189

caccgtttta ttgaataaga cagtgaaaca tctctc 36

<210> SEQ ID NO 190

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 190

ccagaaggca agaaaatccg ccaa 24

<210> SEQ ID NO 191

<211> LENGTH: 33

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 191

agaagcgctt ggaaaagaga agttttatag tgt 33

<210> SEQ ID NO 192

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 192

tgaccttaac aaaggcgaga catctttcca 30

<210> SEQ ID NO 193

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 193

cgcttgacac ttccaagaaa agcagttc 28

<210> SEQ ID NO 194

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 194

cagtcctatc aagagtgaca gttccttcca 30

<210> SEQ ID NO 195

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 195

gaacttccag aaatccacca gttccttcaa 30

<210> SEQ ID NO 196

<211> LENGTH: 33

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 196

gctaaaagcc acattaacaa agaaggaaag ctt 33

<210> SEQ ID NO 197

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 197

ctcgctggat aaatcatcag gacgtagtac 30

<210> SEQ ID NO 198

<211> LENGTH: 22

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 198

tcgctacgga acgctacagc tt 22

<210> SEQ ID NO 199

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 199

ctccttcaat aaaagtgcca agcagttctc 30

<210> SEQ ID NO 200

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 200

cactcttaat accaaggagg gttacagcta 30

<210> SEQ ID NO 201

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 201

tcagtttgga gaagcaaaaa agaacagctc 30

<210> SEQ ID NO 202

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 202

gatcatcaca gaagacagaa agtccagcac 30

<210> SEQ ID NO 203

<211> LENGTH: 29

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 203

gcaatcgctg aagacagaaa gtccagtac 29

<210> SEQ ID NO 204

<211> LENGTH: 29

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 204

cagtttggtg atgcaagaaa ggacagttc 29

<210> SEQ ID NO 205

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 205

cagtcaaagc tgaggaactt tatggcca 28

<210> SEQ ID NO 206

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 206

cttcttaaac aaaagtgcca agcacctctc 30

<210> SEQ ID NO 207

<211> LENGTH: 34

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 207

ctgcttcatt taatgaaaaa aagcagcaaa gctc 34

<210> SEQ ID NO 208

<211> LENGTH: 36

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 208

ttctgtgagc ttccagaaaa caactaaaac tattca 36

<210> SEQ ID NO 209

<211> LENGTH: 36

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 209

cactgtactg ttgaataaaa atgctaaaca tgtctc 36

<210> SEQ ID NO 210

<211> LENGTH: 36

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 210

gcctgtgaac tttgaaaaaa agaaaaagtt catcaa 36

<210> SEQ ID NO 211

<211> LENGTH: 31

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 211

ccaagttgga tgagaaaaag cagcaaagtt c 31

<210> SEQ ID NO 212

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 212

tcagtttggt ataaccagaa aggacagctt 30

<210> SEQ ID NO 213

<211> LENGTH: 36

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 213

aagtagcata ttagataaga aagaactttc cagcat 36

<210> SEQ ID NO 214

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 214

caggcttaaa aaaggagacc agcacatttc 30

<210> SEQ ID NO 215

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 215

cttccagaaa gcagccaaat ccttcag 27

<210> SEQ ID NO 216

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 216

tgataccaaa gcccgtctca gcac 24

<210> SEQ ID NO 217

<211> LENGTH: 31

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 217

gaggcggaaa tattaaagac aaaaactccc c 31

<210> SEQ ID NO 218

<211> LENGTH: 31

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer

<400> SEQUENCE: 218

ccacaataaa catacaggaa aagcacagct c 31

<210> SEQ ID NO 219

<211> LENGTH: 22

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRD V-segment Forward Primer

<400> SEQUENCE: 219

agaaagcagc gaaatccgtc gc 22

<210> SEQ ID NO 220

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRD V-segment Forward Primer

<400> SEQUENCE: 220

tgacattgat attgcaaaga acctggctgt 30

<210> SEQ ID NO 221

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRD V-segment Forward Primer

<400> SEQUENCE: 221

gaaacacatt ctgacccaga aagcctttca 30

<210> SEQ ID NO 222

<211> LENGTH: 55

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 222

gggctggcaa gccacgtttg gtgtcattcc ttagtcgctc tgatagttat ggtta 55

<210> SEQ ID NO 223

<211> LENGTH: 54

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 223

gggctggcaa gccacgtttg gtgcattcct tagtcggtct aaagggtaca gtta 54

<210> SEQ ID NO 224

<211> LENGTH: 54

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 224

gggctggcaa gccacgtttg gtgacaacat gacctatgaa cggttctctt catc 54

<210> SEQ ID NO 225

<211> LENGTH: 54

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 225

gggctggcaa gccacgtttg gtgctgaatt taacaagagc caaacctcct tcca 54

<210> SEQ ID NO 226

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 226

gggctggcaa gccacgtttg gtgccgacag aaagtccagc actctgag 48

<210> SEQ ID NO 227

<211> LENGTH: 59

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 227

gggctggcaa gccacgtttg gtgcactgtt ctattgaata aaaaggataa acatctgtc 59

<210> SEQ ID NO 228

<211> LENGTH: 55

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 228

gggctggcaa gccacgtttg gtggtcacct ttgataccac ccttaaacag agttt 55

<210> SEQ ID NO 229

<211> LENGTH: 58

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 229

gggctggcaa gccacgtttg gtgagactaa atgctacatt actgaagaat ggaagcag 58

<210> SEQ ID NO 230

<211> LENGTH: 59

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 230

gggctggcaa gccacgtttg gtgtgaggct gaatttataa agagtaaatt ctcctttaa 59

<210> SEQ ID NO 231

<211> LENGTH: 55

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 231

gggctggcaa gccacgtttg gtggctgaat ttaagaagag tgaaacctcc ttcca 55

<210> SEQ ID NO 232

<211> LENGTH: 56

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 232

gggctggcaa gccacgtttg gtgggctgaa tttaagagga gtcaatcttc cttcaa 56

<210> SEQ ID NO 233

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 233

gggctggcaa gccacgtttg gtggacactt atcacttccc caatcaatac ccc 53

<210> SEQ ID NO 234

<211> LENGTH: 56

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 234

gggctggcaa gccacgtttg gtgggctgaa tttaacaaga gtcaaacttc cttcca 56

<210> SEQ ID NO 235

<211> LENGTH: 55

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 235

gggctggcaa gccacgtttg gtggctgaat ttaagaagag cgaaacctcc ttcta 55

<210> SEQ ID NO 236

<211> LENGTH: 54

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 236

gggctggcaa gccacgtttg gtgccatgta ccgtaaagaa accacttctt tcca 54

<210> SEQ ID NO 237

<211> LENGTH: 54

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 237

gggctggcaa gccacgtttg gtgccacata ccgtaaagaa accacttctt tcca 54

<210> SEQ ID NO 238

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 238

gggctggcaa gccacgtttg gtgtggatgc agacacaaag caaagctc 48

<210> SEQ ID NO 239

<211> LENGTH: 59

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 239

gggctggcaa gccacgtttg gtgtaaagaa ctgcttggaa aagaaaaatt ttatagtgt 59

<210> SEQ ID NO 240

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 240

gggctggcaa gccacgtttg gtgacagctc aatagagcca gccagtatat ttc 53

<210> SEQ ID NO 241

<211> LENGTH: 52

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 241

gggctggcaa gccacgtttg gtgcagctca ataaagccag ccagtatgtt tc 52

<210> SEQ ID NO 242

<211> LENGTH: 55

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 242

gggctggcaa gccacgtttg gtggcacagg tcgataaatc cagcaagtat atctc 55

<210> SEQ ID NO 243

<211> LENGTH: 57

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 243

gggctggcaa gccacgtttg gtgctgttac attgaacaag acagccaaac atttctc 57

<210> SEQ ID NO 244

<211> LENGTH: 59

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 244

gggctggcaa gccacgtttg gtgcaccgtt ttattgaata agacagtgaa acatctctc 59

<210> SEQ ID NO 245

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 245

gggctggcaa gccacgtttg gtgccagaag gcaagaaaat ccgccaa 47

<210> SEQ ID NO 246

<211> LENGTH: 56

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 246

gggctggcaa gccacgtttg gtgagaagcg cttggaaaag agaagtttta tagtgt 56

<210> SEQ ID NO 247

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 247

gggctggcaa gccacgtttg gtgtgacctt aacaaaggcg agacatcttt cca 53

<210> SEQ ID NO 248

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 248

gggctggcaa gccacgtttg gtgcgcttga cacttccaag aaaagcagtt c 51

<210> SEQ ID NO 249

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 249

gggctggcaa gccacgtttg gtgcagtcct atcaagagtg acagttcctt cca 53

<210> SEQ ID NO 250

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 250

gggctggcaa gccacgtttg gtggaacttc cagaaatcca ccagttcctt caa 53

<210> SEQ ID NO 251

<211> LENGTH: 56

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 251

gggctggcaa gccacgtttg gtggctaaaa gccacattaa caaagaagga aagctt 56

<210> SEQ ID NO 252

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 252

gggctggcaa gccacgtttg gtgctcgctg gataaatcat caggacgtag tac 53

<210> SEQ ID NO 253

<211> LENGTH: 45

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 253

gggctggcaa gccacgtttg gtgtcgctac ggaacgctac agctt 45

<210> SEQ ID NO 254

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 254

gggctggcaa gccacgtttg gtgctccttc aataaaagtg ccaagcagtt ctc 53

<210> SEQ ID NO 255

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 255

gggctggcaa gccacgtttg gtgcactctt aataccaagg agggttacag cta 53

<210> SEQ ID NO 256

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 256

gggctggcaa gccacgtttg gtgtcagttt ggagaagcaa aaaagaacag ctc 53

<210> SEQ ID NO 257

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 257

gggctggcaa gccacgtttg gtggatcatc acagaagaca gaaagtccag cac 53

<210> SEQ ID NO 258

<211> LENGTH: 52

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 258

gggctggcaa gccacgtttg gtggcaatcg ctgaagacag aaagtccagt ac 52

<210> SEQ ID NO 259

<211> LENGTH: 52

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 259

gggctggcaa gccacgtttg gtgcagtttg gtgatgcaag aaaggacagt tc 52

<210> SEQ ID NO 260

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 260

gggctggcaa gccacgtttg gtgcagtcaa agctgaggaa ctttatggcc a 51

<210> SEQ ID NO 261

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 261

gggctggcaa gccacgtttg gtgcttctta aacaaaagtg ccaagcacct ctc 53

<210> SEQ ID NO 262

<211> LENGTH: 57

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 262

gggctggcaa gccacgtttg gtgctgcttc atttaatgaa aaaaagcagc aaagctc 57

<210> SEQ ID NO 263

<211> LENGTH: 59

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 263

gggctggcaa gccacgtttg gtgttctgtg agcttccaga aaacaactaa aactattca 59

<210> SEQ ID NO 264

<211> LENGTH: 59

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 264

gggctggcaa gccacgtttg gtgcactgta ctgttgaata aaaatgctaa acatgtctc 59

<210> SEQ ID NO 265

<211> LENGTH: 59

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 265

gggctggcaa gccacgtttg gtggcctgtg aactttgaaa aaaagaaaaa gttcatcaa 59

<210> SEQ ID NO 266

<211> LENGTH: 54

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 266

gggctggcaa gccacgtttg gtgccaagtt ggatgagaaa aagcagcaaa gttc 54

<210> SEQ ID NO 267

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 267

gggctggcaa gccacgtttg gtgtcagttt ggtataacca gaaaggacag ctt 53

<210> SEQ ID NO 268

<211> LENGTH: 59

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 268

gggctggcaa gccacgtttg gtgaagtagc atattagata agaaagaact ttccagcat 59

<210> SEQ ID NO 269

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 269

gggctggcaa gccacgtttg gtgcaggctt aaaaaaggag accagcacat ttc 53

<210> SEQ ID NO 270

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 270

gggctggcaa gccacgtttg gtgcttccag aaagcagcca aatccttcag 50

<210> SEQ ID NO 271

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 271

gggctggcaa gccacgtttg gtgtgatacc aaagcccgtc tcagcac 47

<210> SEQ ID NO 272

<211> LENGTH: 54

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 272

gggctggcaa gccacgtttg gtggaggcgg aaatattaaa gacaaaaact cccc 54

<210> SEQ ID NO 273

<211> LENGTH: 54

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 273

gggctggcaa gccacgtttg gtgccacaat aaacatacag gaaaagcaca gctc 54

<210> SEQ ID NO 274

<211> LENGTH: 45

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRD V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 274

gggctggcaa gccacgtttg gtgagaaagc agcgaaatcc gtcgc 45

<210> SEQ ID NO 275

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRD V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 275

gggctggcaa gccacgtttg gtgtgacatt gatattgcaa agaacctggc tgt 53

<210> SEQ ID NO 276

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRD V-segment Forward Primer With Universal Adaptor

<400> SEQUENCE: 276

gggctggcaa gccacgtttg gtggaaacac attctgaccc agaaagcctt tca 53

<210> SEQ ID NO 277

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 277

ggggagaagt ggaaactctg gttcc 25

<210> SEQ ID NO 278

<211> LENGTH: 32

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 278

gactcaccag atataatgaa tacatgggtc cc 32

<210> SEQ ID NO 279

<211> LENGTH: 21

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 279

ccggatgctg agtctggtcc c 21

<210> SEQ ID NO 280

<211> LENGTH: 20

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 280

tgtacagcca gcctggtccc 20

<210> SEQ ID NO 281

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 281

cttggttgca cttggagtct tgttcc 26

<210> SEQ ID NO 282

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 282

cacggatgaa caataaggct ggttcc 26

<210> SEQ ID NO 283

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 283

ttggtatgac caccacttgg ttccc 25

<210> SEQ ID NO 284

<211> LENGTH: 21

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 284

actgaccaga agtcgggtgc c 21

<210> SEQ ID NO 285

<211> LENGTH: 36

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 285

atggaactta cttgctttaa caaatagtct tgttcc 36

<210> SEQ ID NO 286

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 286

actgagttcc acttttagct gagtgcc 27

<210> SEQ ID NO 287

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 287

acctggagag actagaagca tagtccc 27

<210> SEQ ID NO 288

<211> LENGTH: 21

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 288

cctgaccagc agtctggtcc c 21

<210> SEQ ID NO 289

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 289

cttgggatga cttggagctt tgttcc 26

<210> SEQ ID NO 290

<211> LENGTH: 34

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 290

cctacttacc aggttttact gataatcttg tccc 34

<210> SEQ ID NO 291

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 291

ctggaactca ctgataaggt ggttcccttc 30

<210> SEQ ID NO 292

<211> LENGTH: 32

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 292

tacttactaa gatccacctt taacatggtt cc 32

<210> SEQ ID NO 293

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 293

acttggttta actagcaccc tggttcc 27

<210> SEQ ID NO 294

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 294

aggccagaca gtcaactgag ttcc 24

<210> SEQ ID NO 295

<211> LENGTH: 34

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 295

ctcacttact tggagtgaca ttatgtttgg atcc 34

<210> SEQ ID NO 296

<211> LENGTH: 32

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 296

tacttacttg ctcttacagt tactgtggtt cc 32

<210> SEQ ID NO 297

<211> LENGTH: 33

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 297

taacttactt ggttttacat tgagtttggt ccc 33

<210> SEQ ID NO 298

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 298

taccaggtaa aacagtcaat tgtgtccc 28

<210> SEQ ID NO 299

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 299

actgggtttc acagataact ccgttcc 27

<210> SEQ ID NO 300

<211> LENGTH: 21

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 300

ggtgaccaca acctgggtcc c 21

<210> SEQ ID NO 301

<211> LENGTH: 22

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 301

gcttgacaag cagccttgtc cc 22

<210> SEQ ID NO 302

<211> LENGTH: 22

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 302

gcagcacgga caatctggtt cc 22

<210> SEQ ID NO 303

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 303

ggcttcacag tgagcgtagt ccc 23

<210> SEQ ID NO 304

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 304

cttggtatga ccgagagttt ggtccc 26

<210> SEQ ID NO 305

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 305

acttgcaatc acagaaagtc ttgtgcc 27

<210> SEQ ID NO 306

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 306

ctggggagaa tatgaagtcg tgtccc 26

<210> SEQ ID NO 307

<211> LENGTH: 22

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 307

gcttcaccac cagctgagtt cc 22

<210> SEQ ID NO 308

<211> LENGTH: 21

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 308

ctggacagca agcagagtgc c 21

<210> SEQ ID NO 309

<211> LENGTH: 32

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 309

gacttacctg gctttataat tagcttggtc cc 32

<210> SEQ ID NO 310

<211> LENGTH: 32

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 310

tacttacttg gaaagacttg taatctggtc cc 32

<210> SEQ ID NO 311

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 311

cttacgtggt aaaacaatca cttgagtgcc 30

<210> SEQ ID NO 312

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 312

ggggaataac ggtgagtctc gttcc 25

<210> SEQ ID NO 313

<211> LENGTH: 31

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 313

cctacctggt tttacttggt aaagttgtcc c 31

<210> SEQ ID NO 314

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 314

cggatttact gccaggcttg ttcc 24

<210> SEQ ID NO 315

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 315

cggggtttga ccattaacct tgttcc 26

<210> SEQ ID NO 316

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 316

gctaaaacct tcagcctggt gcc 23

<210> SEQ ID NO 317

<211> LENGTH: 20

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 317

gtgaccaaca gcgaggtgcc 20

<210> SEQ ID NO 318

<211> LENGTH: 33

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 318

acacttactt ggtttaacag agagtttagt gcc 33

<210> SEQ ID NO 319

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 319

acttggtttt actgtcagtc tggtccc 27

<210> SEQ ID NO 320

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 320

cgagcgtgac ctgaagtctt gttcc 25

<210> SEQ ID NO 321

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 321

cagggctgga tgattagatg agtccc 26

<210> SEQ ID NO 322

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 322

gggcctaact gctaaacgag tccc 24

<210> SEQ ID NO 323

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 323

cacaggactt gactctcaga atggttcc 28

<210> SEQ ID NO 324

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 324

actgggtatg atggtgagtc ttgttcc 27

<210> SEQ ID NO 325

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 325

cttggaatga ccgtcaaact tgtccc 26

<210> SEQ ID NO 326

<211> LENGTH: 31

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 326

acttacttgg aatgactgat aagcttgtcc c 31

<210> SEQ ID NO 327

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 327

tgcttggctt cacagttagt catgtctc 28

<210> SEQ ID NO 328

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 328

cttggatgga cagtcaagat ggtccc 26

<210> SEQ ID NO 329

<211> LENGTH: 32

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 329

aacttacttg gattcacggt taagagagtt cc 32

<210> SEQ ID NO 330

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 330

ttgggttgat agtcagcctg gttcc 25

<210> SEQ ID NO 331

<211> LENGTH: 36

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 331

ccacacttac ttggatttat ttttgtactc atcccc 36

<210> SEQ ID NO 332

<211> LENGTH: 35

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 332

taaaacatac ctggtctaac actcagagtt attcc 35

<210> SEQ ID NO 333

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 333

tacatgggtt tactgtcagt ttcgttcc 28

<210> SEQ ID NO 334

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 334

ccaggattca ctgtgagctg tgttcc 26

<210> SEQ ID NO 335

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 335

agcttcactc tcacttgcgt ccc 23

<210> SEQ ID NO 336

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 336

cccaggctca caattaactc agtccc 26

<210> SEQ ID NO 337

<211> LENGTH: 32

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer

<400> SEQUENCE: 337

tacttgctga gtttcatgat tcctctagtg tt 32

<210> SEQ ID NO 338

<211> LENGTH: 22

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRD J-segment Reverse Primer

<400> SEQUENCE: 338

gttccacagt cacacgggtt cc 22

<210> SEQ ID NO 339

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRD J-segment Reverse Primer

<400> SEQUENCE: 339

ctggttccac gatgagttgt gttcc 25

<210> SEQ ID NO 340

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRD J-segment Reverse Primer

<400> SEQUENCE: 340

ggctccacga agagtttgat gcc 23

<210> SEQ ID NO 341

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRD J-segment Reverse Primer

<400> SEQUENCE: 341

cttacgttgt tgtacctcca gataggttcc 30

<210> SEQ ID NO 342

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 342

ccgggagctg catgtgtcag aggggggaga agtggaaact ctggttcc 48

<210> SEQ ID NO 343

<211> LENGTH: 55

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 343

ccgggagctg catgtgtcag agggactcac cagatataat gaatacatgg gtccc 55

<210> SEQ ID NO 344

<211> LENGTH: 44

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 344

ccgggagctg catgtgtcag aggccggatg ctgagtctgg tccc 44

<210> SEQ ID NO 345

<211> LENGTH: 43

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 345

ccgggagctg catgtgtcag aggtgtacag ccagcctggt ccc 43

<210> SEQ ID NO 346

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 346

ccgggagctg catgtgtcag aggcttggtt gcacttggag tcttgttcc 49

<210> SEQ ID NO 347

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 347

ccgggagctg catgtgtcag aggcacggat gaacaataag gctggttcc 49

<210> SEQ ID NO 348

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 348

ccgggagctg catgtgtcag aggttggtat gaccaccact tggttccc 48

<210> SEQ ID NO 349

<211> LENGTH: 44

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 349

ccgggagctg catgtgtcag aggactgacc agaagtcggg tgcc 44

<210> SEQ ID NO 350

<211> LENGTH: 59

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 350

ccgggagctg catgtgtcag aggatggaac ttacttgctt taacaaatag tcttgttcc 59

<210> SEQ ID NO 351

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 351

ccgggagctg catgtgtcag aggactgagt tccactttta gctgagtgcc 50

<210> SEQ ID NO 352

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 352

ccgggagctg catgtgtcag aggacctgga gagactagaa gcatagtccc 50

<210> SEQ ID NO 353

<211> LENGTH: 44

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 353

ccgggagctg catgtgtcag aggcctgacc agcagtctgg tccc 44

<210> SEQ ID NO 354

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 354

ccgggagctg catgtgtcag aggcttggga tgacttggag ctttgttcc 49

<210> SEQ ID NO 355

<211> LENGTH: 57

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 355

ccgggagctg catgtgtcag aggcctactt accaggtttt actgataatc ttgtccc 57

<210> SEQ ID NO 356

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 356

ccgggagctg catgtgtcag aggctggaac tcactgataa ggtggttccc ttc 53

<210> SEQ ID NO 357

<211> LENGTH: 55

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 357

ccgggagctg catgtgtcag aggtacttac taagatccac ctttaacatg gttcc 55

<210> SEQ ID NO 358

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 358

ccgggagctg catgtgtcag aggacttggt ttaactagca ccctggttcc 50

<210> SEQ ID NO 359

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 359

ccgggagctg catgtgtcag aggaggccag acagtcaact gagttcc 47

<210> SEQ ID NO 360

<211> LENGTH: 57

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 360

ccgggagctg catgtgtcag aggctcactt acttggagtg acattatgtt tggatcc 57

<210> SEQ ID NO 361

<211> LENGTH: 55

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 361

ccgggagctg catgtgtcag aggtacttac ttgctcttac agttactgtg gttcc 55

<210> SEQ ID NO 362

<211> LENGTH: 56

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 362

ccgggagctg catgtgtcag aggtaactta cttggtttta cattgagttt ggtccc 56

<210> SEQ ID NO 363

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 363

ccgggagctg catgtgtcag aggtaccagg taaaacagtc aattgtgtcc c 51

<210> SEQ ID NO 364

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 364

ccgggagctg catgtgtcag aggactgggt ttcacagata actccgttcc 50

<210> SEQ ID NO 365

<211> LENGTH: 44

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 365

ccgggagctg catgtgtcag aggggtgacc acaacctggg tccc 44

<210> SEQ ID NO 366

<211> LENGTH: 45

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 366

ccgggagctg catgtgtcag agggcttgac aagcagcctt gtccc 45

<210> SEQ ID NO 367

<211> LENGTH: 45

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 367

ccgggagctg catgtgtcag agggcagcac ggacaatctg gttcc 45

<210> SEQ ID NO 368

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 368

ccgggagctg catgtgtcag aggggcttca cagtgagcgt agtccc 46

<210> SEQ ID NO 369

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 369

ccgggagctg catgtgtcag aggcttggta tgaccgagag tttggtccc 49

<210> SEQ ID NO 370

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 370

ccgggagctg catgtgtcag aggacttgca atcacagaaa gtcttgtgcc 50

<210> SEQ ID NO 371

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 371

ccgggagctg catgtgtcag aggctgggga gaatatgaag tcgtgtccc 49

<210> SEQ ID NO 372

<211> LENGTH: 45

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 372

ccgggagctg catgtgtcag agggcttcac caccagctga gttcc 45

<210> SEQ ID NO 373

<211> LENGTH: 44

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 373

ccgggagctg catgtgtcag aggctggaca gcaagcagag tgcc 44

<210> SEQ ID NO 374

<211> LENGTH: 55

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 374

ccgggagctg catgtgtcag agggacttac ctggctttat aattagcttg gtccc 55

<210> SEQ ID NO 375

<211> LENGTH: 55

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 375

ccgggagctg catgtgtcag aggtacttac ttggaaagac ttgtaatctg gtccc 55

<210> SEQ ID NO 376

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 376

ccgggagctg catgtgtcag aggcttacgt ggtaaaacaa tcacttgagt gcc 53

<210> SEQ ID NO 377

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 377

ccgggagctg catgtgtcag aggggggaat aacggtgagt ctcgttcc 48

<210> SEQ ID NO 378

<211> LENGTH: 54

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 378

ccgggagctg catgtgtcag aggcctacct ggttttactt ggtaaagttg tccc 54

<210> SEQ ID NO 379

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 379

ccgggagctg catgtgtcag aggcggattt actgccaggc ttgttcc 47

<210> SEQ ID NO 380

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 380

ccgggagctg catgtgtcag aggcggggtt tgaccattaa ccttgttcc 49

<210> SEQ ID NO 381

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 381

ccgggagctg catgtgtcag agggctaaaa ccttcagcct ggtgcc 46

<210> SEQ ID NO 382

<211> LENGTH: 43

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 382

ccgggagctg catgtgtcag agggtgacca acagcgaggt gcc 43

<210> SEQ ID NO 383

<211> LENGTH: 56

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 383

ccgggagctg catgtgtcag aggacactta cttggtttaa cagagagttt agtgcc 56

<210> SEQ ID NO 384

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 384

ccgggagctg catgtgtcag aggacttggt tttactgtca gtctggtccc 50

<210> SEQ ID NO 385

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 385

ccgggagctg catgtgtcag aggcgagcgt gacctgaagt cttgttcc 48

<210> SEQ ID NO 386

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 386

ccgggagctg catgtgtcag aggcagggct ggatgattag atgagtccc 49

<210> SEQ ID NO 387

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 387

ccgggagctg catgtgtcag agggggccta actgctaaac gagtccc 47

<210> SEQ ID NO 388

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 388

ccgggagctg catgtgtcag aggcacagga cttgactctc agaatggttc c 51

<210> SEQ ID NO 389

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 389

ccgggagctg catgtgtcag aggactgggt atgatggtga gtcttgttcc 50

<210> SEQ ID NO 390

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 390

ccgggagctg catgtgtcag aggcttggaa tgaccgtcaa acttgtccc 49

<210> SEQ ID NO 391

<211> LENGTH: 54

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 391

ccgggagctg catgtgtcag aggacttact tggaatgact gataagcttg tccc 54

<210> SEQ ID NO 392

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 392

ccgggagctg catgtgtcag aggtgcttgg cttcacagtt agtcatgtct c 51

<210> SEQ ID NO 393

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 393

ccgggagctg catgtgtcag aggcttggat ggacagtcaa gatggtccc 49

<210> SEQ ID NO 394

<211> LENGTH: 55

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 394

ccgggagctg catgtgtcag aggaacttac ttggattcac ggttaagaga gttcc 55

<210> SEQ ID NO 395

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 395

ccgggagctg catgtgtcag aggttgggtt gatagtcagc ctggttcc 48

<210> SEQ ID NO 396

<211> LENGTH: 59

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 396

ccgggagctg catgtgtcag aggccacact tacttggatt tatttttgta ctcatcccc 59

<210> SEQ ID NO 397

<211> LENGTH: 58

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 397

ccgggagctg catgtgtcag aggtaaaaca tacctggtct aacactcaga gttattcc 58

<210> SEQ ID NO 398

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 398

ccgggagctg catgtgtcag aggtacatgg gtttactgtc agtttcgttc c 51

<210> SEQ ID NO 399

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 399

ccgggagctg catgtgtcag aggccaggat tcactgtgag ctgtgttcc 49

<210> SEQ ID NO 400

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 400

ccgggagctg catgtgtcag aggagcttca ctctcacttg cgtccc 46

<210> SEQ ID NO 401

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 401

ccgggagctg catgtgtcag aggcccaggc tcacaattaa ctcagtccc 49

<210> SEQ ID NO 402

<211> LENGTH: 55

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRA J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 402

ccgggagctg catgtgtcag aggtacttgc tgagtttcat gattcctcta gtgtt 55

<210> SEQ ID NO 403

<211> LENGTH: 45

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRD J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 403

ccgggagctg catgtgtcag agggttccac agtcacacgg gttcc 45

<210> SEQ ID NO 404

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRD J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 404

ccgggagctg catgtgtcag aggctggttc cacgatgagt tgtgttcc 48

<210> SEQ ID NO 405

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRD J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 405

ccgggagctg catgtgtcag aggggctcca cgaagagttt gatgcc 46

<210> SEQ ID NO 406

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: TCRD J-segment Reverse Primer With Universal Adaptor

<400> SEQUENCE: 406

ccgggagctg catgtgtcag aggcttacgt tgttgtacct ccagataggt tcc 53

<210> SEQ ID NO 407

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 407

ggcccctgga caagggcttg aaagg 25

<210> SEQ ID NO 408

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 408

gctctcaggg aggggctgca atgg 24

<210> SEQ ID NO 409

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 409

ccagatgccc gggaaagaac tggagtgg 28

<210> SEQ ID NO 410

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 410

aggctccagg gaatgggctg gagttg 26

<210> SEQ ID NO 411

<211> LENGTH: 29

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 411

gccaggctcc agggaaggga ctggaatat 29

<210> SEQ ID NO 412

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 412

gggccctgga caagggcata gtgg 24

<210> SEQ ID NO 413

<211> LENGTH: 38

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 413

cctggatcca ccatctccca tcaaaaggtc ttcagtgt 38

<210> SEQ ID NO 414

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 414

ccccaggaca ggggcttgag tgg 23

<210> SEQ ID NO 415

<211> LENGTH: 31

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 415

tgtgacaaag ccctggacaa gggcatagtg g 31

<210> SEQ ID NO 416

<211> LENGTH: 33

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 416

gtccagcagg attcatggca agtgttggtg agg 33

<210> SEQ ID NO 417

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 417

ccgccagtcc tcagtcaagg gagtgg 26

<210> SEQ ID NO 418

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 418

cccctctctg ggagggaatg gagtgg 26

<210> SEQ ID NO 419

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 419

ctccccaggg aagggactgg agtga 25

<210> SEQ ID NO 420

<211> LENGTH: 32

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 420

ctgtaggcac tggatggaaa gcacaggagt gg 32

<210> SEQ ID NO 421

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 421

cccccaggga agggaatgga gtgg 24

<210> SEQ ID NO 422

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 422

cccgtgggca acaggctgga atgg 24

<210> SEQ ID NO 423

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 423

ccccccagga agggactgga gtga 24

<210> SEQ ID NO 424

<211> LENGTH: 21

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 424

ccggggaagg gactggagcg a 21

<210> SEQ ID NO 425

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 425

caggcccatg gacaagggct tgaggaa 27

<210> SEQ ID NO 426

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 426

caggctccag ggaacagact ggagtgg 27

<210> SEQ ID NO 427

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 427

agcccccagg gaagaagctg aagtgg 26

<210> SEQ ID NO 428

<211> LENGTH: 31

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 428

ccagcacgct tcacaacagg gataggtgtg g 31

<210> SEQ ID NO 429

<211> LENGTH: 31

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 429

ctgcttgccc ctagaagatg gacaggagtg g 31

<210> SEQ ID NO 430

<211> LENGTH: 33

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 430

gtgtgacagg cccctggata agggtttgaa tgg 33

<210> SEQ ID NO 431

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 431

gcccccatgg gaggtactgg agtgg 25

<210> SEQ ID NO 432

<211> LENGTH: 31

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 432

ccagcacgct tcacaacagg aattggtgtg g 31

<210> SEQ ID NO 433

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 433

cgccaggctc caagaaaggg tttgtagtgg 30

<210> SEQ ID NO 434

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 434

ccgcaaggaa gggactggag cga 23

<210> SEQ ID NO 435

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 435

ccaggctcca ggaaagggtt tggagtgg 28

<210> SEQ ID NO 436

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 436

cagcaggctt cacaacaggg attgtggtgg 30

<210> SEQ ID NO 437

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 437

cacgagtcca catgggaagg actggagtgg 30

<210> SEQ ID NO 438

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 438

tctccaggca aggggctgga gtga 24

<210> SEQ ID NO 439

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 439

ccgggcttca agaaaggggc tggaatgg 28

<210> SEQ ID NO 440

<211> LENGTH: 31

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 440

caatgagact ctagggaagg ggctggaggg a 31

<210> SEQ ID NO 441

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 441

cactagcccg cttccatggg ctgt 24

<210> SEQ ID NO 442

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 442

aggcttcagg acaggggttg gtgtgg 26

<210> SEQ ID NO 443

<211> LENGTH: 31

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 443

ccgccaagct acaggaaaag gtctggagtg g 31

<210> SEQ ID NO 444

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 444

gctccaggaa aggggctgca gtga 24

<210> SEQ ID NO 445

<211> LENGTH: 20

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 445

ctgggaaggc gctggggcac 20

<210> SEQ ID NO 446

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 446

tgatagaccc ctggacaggg ctttgagtgg 30

<210> SEQ ID NO 447

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 447

ggctcgtgga caacgccttg agtgg 25

<210> SEQ ID NO 448

<211> LENGTH: 22

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 448

cccagggaag gggctggagt gg 22

<210> SEQ ID NO 449

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 449

gctccaggga aggggctgga gtga 24

<210> SEQ ID NO 450

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 450

ggcccatgca caagggcttg agtgg 25

<210> SEQ ID NO 451

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 451

ctgggggaca gaggctggag tgg 23

<210> SEQ ID NO 452

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 452

ccccagggaa ggggctggaa tgg 23

<210> SEQ ID NO 453

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 453

ccccaggaaa ggccctggag tgg 23

<210> SEQ ID NO 454

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 454

cagtccccat cgagaggcct tgagtgg 27

<210> SEQ ID NO 455

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 455

ctgcaggcaa ggggctggag tga 23

<210> SEQ ID NO 456

<211> LENGTH: 29

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 456

gacaggctcc tggaaaaggg cttgagtgg 29

<210> SEQ ID NO 457

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 457

ggccactgga caagggcttg agtgg 25

<210> SEQ ID NO 458

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 458

caggcttctg ggaaagggct ggagtgg 27

<210> SEQ ID NO 459

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 459

ggcccctgga aaagggcttg agtgg 25

<210> SEQ ID NO 460

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 460

gcccccggac aaaggcttga gtgg 24

<210> SEQ ID NO 461

<211> LENGTH: 22

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 461

cgccgggaag ggactggagt gg 22

<210> SEQ ID NO 462

<211> LENGTH: 32

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 462

tccagcaggc ttcatgacag ggatttgtgt gg 32

<210> SEQ ID NO 463

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 463

cagtcccctg gacaagggct tgagtgg 27

<210> SEQ ID NO 464

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 464

cagcaggctt catgacaggg attggtgtgg 30

<210> SEQ ID NO 465

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 465

gcctcaggga aggggctgga atgg 24

<210> SEQ ID NO 466

<211> LENGTH: 22

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 466

tcccgggaag gggctggaat gg 22

<210> SEQ ID NO 467

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 467

gcccctggac aagggcttga gtgg 24

<210> SEQ ID NO 468

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 468

ctccggggaa gggtctggag tgg 23

<210> SEQ ID NO 469

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 469

ctccaggcaa ggggctggag tgg 23

<210> SEQ ID NO 470

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 470

cccccggaca agcgcttgag tgg 23

<210> SEQ ID NO 471

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 471

gatgcccggg aaaggcttgg agtgg 25

<210> SEQ ID NO 472

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 472

gctccaggga agggtctgca gtgg 24

<210> SEQ ID NO 473

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 473

ctccagggaa ggggctggtg tgg 23

<210> SEQ ID NO 474

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 474

gcaggcttca tgacaggggt gggtgtag 28

<210> SEQ ID NO 475

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 475

gcttccggga aagggctgga gtgg 24

<210> SEQ ID NO 476

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 476

gctggaggag aggggctgga gtgg 24

<210> SEQ ID NO 477

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 477

catcaggctc ctgggaaagg gctggagtag 30

<210> SEQ ID NO 478

<211> LENGTH: 20

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 478

ccgggaaggg gctggagtgg 20

<210> SEQ ID NO 479

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 479

caccagggaa gggcctggag tgg 23

<210> SEQ ID NO 480

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 480

ctccggagaa ggggctggag tgg 23

<210> SEQ ID NO 481

<211> LENGTH: 22

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 481

cccagggaag gccctggagt gg 22

<210> SEQ ID NO 482

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 482

ccctcagcaa aggccctgga gtgg 24

<210> SEQ ID NO 483

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 483

caagctccag ggaagggact ggagtgg 27

<210> SEQ ID NO 484

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 484

ctccagggaa ggggctggag tgg 23

<210> SEQ ID NO 485

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 485

ctccagggaa gggtccggag tgg 23

<210> SEQ ID NO 486

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 486

gctccaggaa aggggctgga gtgg 24

<210> SEQ ID NO 487

<211> LENGTH: 22

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 487

cccagggaag ggcctggagt gg 22

<210> SEQ ID NO 488

<211> LENGTH: 22

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 488

gcccgggaaa ggcctggagt gg 22

<210> SEQ ID NO 489

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 489

ctccagggaa gggcctggag tgg 23

<210> SEQ ID NO 490

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 490

aggcttcatg acaggggtgg gtgtgg 26

<210> SEQ ID NO 491

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 491

ccccagggaa gggactggag tgg 23

<210> SEQ ID NO 492

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer

<400> SEQUENCE: 492

ggctccaggg aagggtctgg agtgg 25

<210> SEQ ID NO 493

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 493

gggctggcaa gccacgtttg gtgggcccct ggacaagggc ttgaaagg 48

<210> SEQ ID NO 494

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 494

gggctggcaa gccacgtttg gtggctctca gggaggggct gcaatgg 47

<210> SEQ ID NO 495

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 495

gggctggcaa gccacgtttg gtgccagatg cccgggaaag aactggagtg g 51

<210> SEQ ID NO 496

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 496

gggctggcaa gccacgtttg gtgaggctcc agggaatggg ctggagttg 49

<210> SEQ ID NO 497

<211> LENGTH: 52

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 497

gggctggcaa gccacgtttg gtggccaggc tccagggaag ggactggaat at 52

<210> SEQ ID NO 498

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 498

gggctggcaa gccacgtttg gtggggccct ggacaagggc atagtgg 47

<210> SEQ ID NO 499

<211> LENGTH: 61

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 499

gggctggcaa gccacgtttg gtgcctggat ccaccatctc ccatcaaaag gtcttcagtg 60

t 61

<210> SEQ ID NO 500

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 500

gggctggcaa gccacgtttg gtgccccagg acaggggctt gagtgg 46

<210> SEQ ID NO 501

<211> LENGTH: 54

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 501

gggctggcaa gccacgtttg gtgtgtgaca aagccctgga caagggcata gtgg 54

<210> SEQ ID NO 502

<211> LENGTH: 56

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 502

gggctggcaa gccacgtttg gtggtccagc aggattcatg gcaagtgttg gtgagg 56

<210> SEQ ID NO 503

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 503

gggctggcaa gccacgtttg gtgccgccag tcctcagtca agggagtgg 49

<210> SEQ ID NO 504

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 504

gggctggcaa gccacgtttg gtgcccctct ctgggaggga atggagtgg 49

<210> SEQ ID NO 505

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 505

gggctggcaa gccacgtttg gtgctcccca gggaagggac tggagtga 48

<210> SEQ ID NO 506

<211> LENGTH: 55

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 506

gggctggcaa gccacgtttg gtgctgtagg cactggatgg aaagcacagg agtgg 55

<210> SEQ ID NO 507

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 507

gggctggcaa gccacgtttg gtgcccccag ggaagggaat ggagtgg 47

<210> SEQ ID NO 508

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 508

gggctggcaa gccacgtttg gtgcccgtgg gcaacaggct ggaatgg 47

<210> SEQ ID NO 509

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 509

gggctggcaa gccacgtttg gtgcccccca ggaagggact ggagtga 47

<210> SEQ ID NO 510

<211> LENGTH: 44

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 510

gggctggcaa gccacgtttg gtgccgggga agggactgga gcga 44

<210> SEQ ID NO 511

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 511

gggctggcaa gccacgtttg gtgcaggccc atggacaagg gcttgaggaa 50

<210> SEQ ID NO 512

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 512

gggctggcaa gccacgtttg gtgcaggctc cagggaacag actggagtgg 50

<210> SEQ ID NO 513

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 513

gggctggcaa gccacgtttg gtgagccccc agggaagaag ctgaagtgg 49

<210> SEQ ID NO 514

<211> LENGTH: 54

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 514

gggctggcaa gccacgtttg gtgccagcac gcttcacaac agggataggt gtgg 54

<210> SEQ ID NO 515

<211> LENGTH: 54

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 515

gggctggcaa gccacgtttg gtgctgcttg cccctagaag atggacagga gtgg 54

<210> SEQ ID NO 516

<211> LENGTH: 56

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 516

gggctggcaa gccacgtttg gtggtgtgac aggcccctgg ataagggttt gaatgg 56

<210> SEQ ID NO 517

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 517

gggctggcaa gccacgtttg gtggccccca tgggaggtac tggagtgg 48

<210> SEQ ID NO 518

<211> LENGTH: 54

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 518

gggctggcaa gccacgtttg gtgccagcac gcttcacaac aggaattggt gtgg 54

<210> SEQ ID NO 519

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 519

gggctggcaa gccacgtttg gtgcgccagg ctccaagaaa gggtttgtag tgg 53

<210> SEQ ID NO 520

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 520

gggctggcaa gccacgtttg gtgccgcaag gaagggactg gagcga 46

<210> SEQ ID NO 521

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 521

gggctggcaa gccacgtttg gtgccaggct ccaggaaagg gtttggagtg g 51

<210> SEQ ID NO 522

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 522

gggctggcaa gccacgtttg gtgcagcagg cttcacaaca gggattgtgg tgg 53

<210> SEQ ID NO 523

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 523

gggctggcaa gccacgtttg gtgcacgagt ccacatggga aggactggag tgg 53

<210> SEQ ID NO 524

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 524

gggctggcaa gccacgtttg gtgtctccag gcaaggggct ggagtga 47

<210> SEQ ID NO 525

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 525

gggctggcaa gccacgtttg gtgccgggct tcaagaaagg ggctggaatg g 51

<210> SEQ ID NO 526

<211> LENGTH: 54

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 526

gggctggcaa gccacgtttg gtgcaatgag actctaggga aggggctgga ggga 54

<210> SEQ ID NO 527

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 527

gggctggcaa gccacgtttg gtgcactagc ccgcttccat gggctgt 47

<210> SEQ ID NO 528

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 528

gggctggcaa gccacgtttg gtgaggcttc aggacagggg ttggtgtgg 49

<210> SEQ ID NO 529

<211> LENGTH: 54

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 529

gggctggcaa gccacgtttg gtgccgccaa gctacaggaa aaggtctgga gtgg 54

<210> SEQ ID NO 530

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 530

gggctggcaa gccacgtttg gtggctccag gaaaggggct gcagtga 47

<210> SEQ ID NO 531

<211> LENGTH: 43

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 531

gggctggcaa gccacgtttg gtgctgggaa ggcgctgggg cac 43

<210> SEQ ID NO 532

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 532

gggctggcaa gccacgtttg gtgtgataga cccctggaca gggctttgag tgg 53

<210> SEQ ID NO 533

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 533

gggctggcaa gccacgtttg gtgggctcgt ggacaacgcc ttgagtgg 48

<210> SEQ ID NO 534

<211> LENGTH: 45

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 534

gggctggcaa gccacgtttg gtgcccaggg aaggggctgg agtgg 45

<210> SEQ ID NO 535

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 535

gggctggcaa gccacgtttg gtggctccag ggaaggggct ggagtga 47

<210> SEQ ID NO 536

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 536

gggctggcaa gccacgtttg gtgggcccat gcacaagggc ttgagtgg 48

<210> SEQ ID NO 537

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 537

gggctggcaa gccacgtttg gtgctggggg acagaggctg gagtgg 46

<210> SEQ ID NO 538

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 538

gggctggcaa gccacgtttg gtgccccagg gaaggggctg gaatgg 46

<210> SEQ ID NO 539

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 539

gggctggcaa gccacgtttg gtgccccagg aaaggccctg gagtgg 46

<210> SEQ ID NO 540

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 540

gggctggcaa gccacgtttg gtgcagtccc catcgagagg ccttgagtgg 50

<210> SEQ ID NO 541

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 541

gggctggcaa gccacgtttg gtgctgcagg caaggggctg gagtga 46

<210> SEQ ID NO 542

<211> LENGTH: 52

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 542

gggctggcaa gccacgtttg gtggacaggc tcctggaaaa gggcttgagt gg 52

<210> SEQ ID NO 543

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 543

gggctggcaa gccacgtttg gtgggccact ggacaagggc ttgagtgg 48

<210> SEQ ID NO 544

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 544

gggctggcaa gccacgtttg gtgcaggctt ctgggaaagg gctggagtgg 50

<210> SEQ ID NO 545

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 545

gggctggcaa gccacgtttg gtgggcccct ggaaaagggc ttgagtgg 48

<210> SEQ ID NO 546

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 546

gggctggcaa gccacgtttg gtggcccccg gacaaaggct tgagtgg 47

<210> SEQ ID NO 547

<211> LENGTH: 45

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 547

gggctggcaa gccacgtttg gtgcgccggg aagggactgg agtgg 45

<210> SEQ ID NO 548

<211> LENGTH: 55

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 548

gggctggcaa gccacgtttg gtgtccagca ggcttcatga cagggatttg tgtgg 55

<210> SEQ ID NO 549

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 549

gggctggcaa gccacgtttg gtgcagtccc ctggacaagg gcttgagtgg 50

<210> SEQ ID NO 550

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 550

gggctggcaa gccacgtttg gtgcagcagg cttcatgaca gggattggtg tgg 53

<210> SEQ ID NO 551

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 551

gggctggcaa gccacgtttg gtggcctcag ggaaggggct ggaatgg 47

<210> SEQ ID NO 552

<211> LENGTH: 45

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 552

gggctggcaa gccacgtttg gtgtcccggg aaggggctgg aatgg 45

<210> SEQ ID NO 553

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 553

gggctggcaa gccacgtttg gtggcccctg gacaagggct tgagtgg 47

<210> SEQ ID NO 554

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 554

gggctggcaa gccacgtttg gtgctccggg gaagggtctg gagtgg 46

<210> SEQ ID NO 555

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 555

gggctggcaa gccacgtttg gtgctccagg caaggggctg gagtgg 46

<210> SEQ ID NO 556

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 556

gggctggcaa gccacgtttg gtgcccccgg acaagcgctt gagtgg 46

<210> SEQ ID NO 557

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 557

gggctggcaa gccacgtttg gtggatgccc gggaaaggct tggagtgg 48

<210> SEQ ID NO 558

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 558

gggctggcaa gccacgtttg gtggctccag ggaagggtct gcagtgg 47

<210> SEQ ID NO 559

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 559

gggctggcaa gccacgtttg gtgctccagg gaaggggctg gtgtgg 46

<210> SEQ ID NO 560

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 560

gggctggcaa gccacgtttg gtggcaggct tcatgacagg ggtgggtgta g 51

<210> SEQ ID NO 561

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 561

gggctggcaa gccacgtttg gtggcttccg ggaaagggct ggagtgg 47

<210> SEQ ID NO 562

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 562

gggctggcaa gccacgtttg gtggctggag gagaggggct ggagtgg 47

<210> SEQ ID NO 563

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 563

gggctggcaa gccacgtttg gtgcatcagg ctcctgggaa agggctggag tag 53

<210> SEQ ID NO 564

<211> LENGTH: 43

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 564

gggctggcaa gccacgtttg gtgccgggaa ggggctggag tgg 43

<210> SEQ ID NO 565

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 565

gggctggcaa gccacgtttg gtgcaccagg gaagggcctg gagtgg 46

<210> SEQ ID NO 566

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 566

gggctggcaa gccacgtttg gtgctccgga gaaggggctg gagtgg 46

<210> SEQ ID NO 567

<211> LENGTH: 45

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 567

gggctggcaa gccacgtttg gtgcccaggg aaggccctgg agtgg 45

<210> SEQ ID NO 568

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 568

gggctggcaa gccacgtttg gtgccctcag caaaggccct ggagtgg 47

<210> SEQ ID NO 569

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 569

gggctggcaa gccacgtttg gtgcaagctc cagggaaggg actggagtgg 50

<210> SEQ ID NO 570

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 570

gggctggcaa gccacgtttg gtgctccagg gaaggggctg gagtgg 46

<210> SEQ ID NO 571

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 571

gggctggcaa gccacgtttg gtgctccagg gaagggtccg gagtgg 46

<210> SEQ ID NO 572

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 572

gggctggcaa gccacgtttg gtggctccag gaaaggggct ggagtgg 47

<210> SEQ ID NO 573

<211> LENGTH: 45

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 573

gggctggcaa gccacgtttg gtgcccaggg aagggcctgg agtgg 45

<210> SEQ ID NO 574

<211> LENGTH: 45

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 574

gggctggcaa gccacgtttg gtggcccggg aaaggcctgg agtgg 45

<210> SEQ ID NO 575

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 575

gggctggcaa gccacgtttg gtgctccagg gaagggcctg gagtgg 46

<210> SEQ ID NO 576

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 576

gggctggcaa gccacgtttg gtgaggcttc atgacagggg tgggtgtgg 49

<210> SEQ ID NO 577

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 577

gggctggcaa gccacgtttg gtgccccagg gaagggactg gagtgg 46

<210> SEQ ID NO 578

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 578

gggctggcaa gccacgtttg gtgggctcca gggaagggtc tggagtgg 48

<210> SEQ ID NO 579

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH J-segment Reverse Primer

<400> SEQUENCE: 579

cacctgagga gacggtgacc agggt 25

<210> SEQ ID NO 580

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH J-segment Reverse Primer

<400> SEQUENCE: 580

actcacctga ggagacagtg accagggt 28

<210> SEQ ID NO 581

<211> LENGTH: 37

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH J-segment Reverse Primer

<400> SEQUENCE: 581

ggaaagccat cttacctgaa gagacggtga ccattgt 37

<210> SEQ ID NO 582

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH J-segment Reverse Primer

<400> SEQUENCE: 582

tcttacctga ggagacggtg accgtggt 28

<210> SEQ ID NO 583

<211> LENGTH: 20

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH J-segment Reverse Primer

<400> SEQUENCE: 583

gtggctgcgg tctccagggc 20

<210> SEQ ID NO 584

<211> LENGTH: 18

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH J-segment Reverse Primer

<400> SEQUENCE: 584

ggagacggtg gccgggct 18

<210> SEQ ID NO 585

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH J-segment Reverse Primer

<400> SEQUENCE: 585

gcctgacccc tcagaagcca gacc 24

<210> SEQ ID NO 586

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH J-segment Reverse Primer with Universal Adaptor

<400> SEQUENCE: 586

ccgggagctg catgtgtcag aggcacctga ggagacggtg accagggt 48

<210> SEQ ID NO 587

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH J-segment Reverse Primer with Universal Adaptor

<400> SEQUENCE: 587

ccgggagctg catgtgtcag aggactcacc tgaggagaca gtgaccaggg t 51

<210> SEQ ID NO 588

<211> LENGTH: 60

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH J-segment Reverse Primer with Universal Adaptor

<400> SEQUENCE: 588

ccgggagctg catgtgtcag aggggaaagc catcttacct gaagagacgg tgaccattgt 60

<210> SEQ ID NO 589

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH J-segment Reverse Primer with Universal Adaptor

<400> SEQUENCE: 589

ccgggagctg catgtgtcag aggtcttacc tgaggagacg gtgaccgtgg t 51

<210> SEQ ID NO 590

<211> LENGTH: 43

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH J-segment Reverse Primer with Universal Adaptor

<400> SEQUENCE: 590

ccgggagctg catgtgtcag agggtggctg cggtctccag ggc 43

<210> SEQ ID NO 591

<211> LENGTH: 41

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH J-segment Reverse Primer with Universal Adaptor

<400> SEQUENCE: 591

ccgggagctg catgtgtcag aggggagacg gtggccgggc t 41

<210> SEQ ID NO 592

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGH J-segment Reverse Primer with Universal Adaptor

<400> SEQUENCE: 592

ccgggagctg catgtgtcag agggcctgac ccctcagaag ccagacc 47

<210> SEQ ID NO 593

<211> LENGTH: 35

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 593

tctgcatctg taggagacag agtcaccatc acttg 35

<210> SEQ ID NO 594

<211> LENGTH: 35

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 594

tctgcatcta caggagacag agtcaccatc acttg 35

<210> SEQ ID NO 595

<211> LENGTH: 35

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 595

ctgcatctgt aaggagacag tgtcaccatc acttg 35

<210> SEQ ID NO 596

<211> LENGTH: 35

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 596

tctgcatcta caggagacag agtcaccatc agttg 35

<210> SEQ ID NO 597

<211> LENGTH: 35

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 597

actgcatctg taggagagag agtcaccatc acttg 35

<210> SEQ ID NO 598

<211> LENGTH: 33

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 598

gcatctgtaa ggagacagcg tcaccatcac ttg 33

<210> SEQ ID NO 599

<211> LENGTH: 36

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 599

gtctgcatct gtaggagaca gagtcagtat catttg 36

<210> SEQ ID NO 600

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 600

ggagagccgg cctccatctc ctg 23

<210> SEQ ID NO 601

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 601

cctggagagc cagcctccat ctcctg 26

<210> SEQ ID NO 602

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 602

ctggagagcc ggcctccatc tcttg 25

<210> SEQ ID NO 603

<211> LENGTH: 31

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 603

tcttccttgg agagccatcc tccatttcct g 31

<210> SEQ ID NO 604

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 604

ggacagccgg cctccatctc ctg 23

<210> SEQ ID NO 605

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 605

tggagagccg gcctccatct cctg 24

<210> SEQ ID NO 606

<211> LENGTH: 36

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 606

ataatatttg tacataactt tgtacttcat ctcctg 36

<210> SEQ ID NO 607

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 607

cccctggaaa gccagcctct atctcctg 28

<210> SEQ ID NO 608

<211> LENGTH: 32

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 608

ctcttccttg gagagccatc ctccatttcc tg 32

<210> SEQ ID NO 609

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 609

ggacagccgg cctccatctc ctt 23

<210> SEQ ID NO 610

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 610

cctggagagc aggcctccat gtcctg 26

<210> SEQ ID NO 611

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 611

ccaggggaaa gagccaccct ctcctg 26

<210> SEQ ID NO 612

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 612

tccaggggaa agagtcaccc tctcctg 27

<210> SEQ ID NO 613

<211> LENGTH: 35

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 613

tctttgtctc tggagaaaaa agccaccctg acttg 35

<210> SEQ ID NO 614

<211> LENGTH: 29

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 614

tctctagggg aaaaagccac cctcaccta 29

<210> SEQ ID NO 615

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 615

ggggaaggag ccaccctcac ctg 23

<210> SEQ ID NO 616

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 616

gggcgagagg gccaccatca actg 24

<210> SEQ ID NO 617

<211> LENGTH: 32

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 617

gcgactccag gagacaaagt caacatctcc tg 32

<210> SEQ ID NO 618

<211> LENGTH: 34

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 618

ctgtgactcc aaaggagaaa gtcaccatca cctg 34

<210> SEQ ID NO 619

<211> LENGTH: 29

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 619

actccagggg agaaagtcac catcacctg 29

<210> SEQ ID NO 620

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 620

caggacagag ggccaccatc acctg 25

<210> SEQ ID NO 621

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 621

cccaggcaga gggtcaccat ctcctg 26

<210> SEQ ID NO 622

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 622

ccagggcaga gggtcaccat ctcctg 26

<210> SEQ ID NO 623

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 623

ccgggcagag ggtcaccatc tcttg 25

<210> SEQ ID NO 624

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 624

ccccaggaca gaaggtcacc atctcctg 28

<210> SEQ ID NO 625

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 625

ccacaaggca gaggctcact gtctcctg 28

<210> SEQ ID NO 626

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 626

gtctcctgga cagtcagtca ccatctcctg 30

<210> SEQ ID NO 627

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 627

gtctcctgga cagtcgatca ccatctcctg 30

<210> SEQ ID NO 628

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 628

tcctggacag tcggtcacca tctcctg 27

<210> SEQ ID NO 629

<211> LENGTH: 33

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 629

ctgggacttg gggtaaacag tcaccatctt ctg 33

<210> SEQ ID NO 630

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 630

ccaggacaga cagccagcat cacctg 26

<210> SEQ ID NO 631

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 631

ctttgggacg tacggccagg atcatctg 28

<210> SEQ ID NO 632

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 632

ctttgggaca gatggccagg atcacctg 28

<210> SEQ ID NO 633

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 633

ccaggacagg cagccatgat cacctg 26

<210> SEQ ID NO 634

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 634

tgggacagag ggccaggatc accta 25

<210> SEQ ID NO 635

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 635

gggacaggcg gccaggatta cctg 24

<210> SEQ ID NO 636

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 636

ccaggacaaa cggccaggat cacctg 26

<210> SEQ ID NO 637

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 637

cacagcacag atggccagga tcacctg 27

<210> SEQ ID NO 638

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 638

ccaggacaga cagccaggat cagctg 26

<210> SEQ ID NO 639

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 639

ccccaggaca gatgaccagg atcacctg 28

<210> SEQ ID NO 640

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 640

ccctaggaca gatggccagg atcacctg 28

<210> SEQ ID NO 641

<211> LENGTH: 31

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 641

gtgtctgtgg acagtcagca agggtaacct g 31

<210> SEQ ID NO 642

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 642

ggccttggga cagacagtca ggatcacatg 30

<210> SEQ ID NO 643

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 643

ccccaggaaa gacggccagg attacctg 28

<210> SEQ ID NO 644

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 644

cccaggacag aaagccagga tcacctg 27

<210> SEQ ID NO 645

<211> LENGTH: 34

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 645

cagtagctcc aggacagatg actaggatca cctg 34

<210> SEQ ID NO 646

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 646

caggacagac ggccaggatc acctg 25

<210> SEQ ID NO 647

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 647

cctgggacag tcagccaggg taacctg 27

<210> SEQ ID NO 648

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 648

cgggacagac agccaggatc acctg 25

<210> SEQ ID NO 649

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 649

cccaggacag acacccagga tcacctg 27

<210> SEQ ID NO 650

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 650

ccccattaca gatggccagg atcacctg 28

<210> SEQ ID NO 651

<211> LENGTH: 29

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 651

gccttgggat agacagccag gatcacctg 29

<210> SEQ ID NO 652

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 652

ccttgggaca aatggccagg atcacctg 28

<210> SEQ ID NO 653

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 653

ctgggagcct cgatcaagct cacctg 26

<210> SEQ ID NO 654

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 654

cctgggatcc tcggtcaagc tcacctg 27

<210> SEQ ID NO 655

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 655

gggagcctcg gtcaagctca cctg 24

<210> SEQ ID NO 656

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 656

tcctggagaa tccgccagac tcacctg 27

<210> SEQ ID NO 657

<211> LENGTH: 29

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 657

tctcctggag catcagccag attcacctg 29

<210> SEQ ID NO 658

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 658

tcctggagca tcagccagtc tcacctg 27

<210> SEQ ID NO 659

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 659

tcctggagca tcagccagac tcacctg 27

<210> SEQ ID NO 660

<211> LENGTH: 32

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 660

gcatcttctg gagcatcagt cagactcacc tg 32

<210> SEQ ID NO 661

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 661

cccaggaggg acagtcactc tcaccta 27

<210> SEQ ID NO 662

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 662

cccaggaggg acagtcactc tcacctg 27

<210> SEQ ID NO 663

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 663

cccctggagg gacagtcaca ctcacttg 28

<210> SEQ ID NO 664

<211> LENGTH: 25

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 664

tgggagcctc ggtcacactc acctg 25

<210> SEQ ID NO 665

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer

<400> SEQUENCE: 665

cttgagacag accgccacac tcacctg 27

<210> SEQ ID NO 666

<211> LENGTH: 37

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer

<400> SEQUENCE: 666

gtaaataatt gcatttttta atgaccgtgg gtctgtg 37

<210> SEQ ID NO 667

<211> LENGTH: 58

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 667

gggctggcaa gccacgtttg gtgtctgcat ctgtaggaga cagagtcacc atcacttg 58

<210> SEQ ID NO 668

<211> LENGTH: 58

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 668

gggctggcaa gccacgtttg gtgtctgcat ctacaggaga cagagtcacc atcacttg 58

<210> SEQ ID NO 669

<211> LENGTH: 58

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 669

gggctggcaa gccacgtttg gtgctgcatc tgtaaggaga cagtgtcacc atcacttg 58

<210> SEQ ID NO 670

<211> LENGTH: 58

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 670

gggctggcaa gccacgtttg gtgtctgcat ctacaggaga cagagtcacc atcagttg 58

<210> SEQ ID NO 671

<211> LENGTH: 58

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 671

gggctggcaa gccacgtttg gtgactgcat ctgtaggaga gagagtcacc atcacttg 58

<210> SEQ ID NO 672

<211> LENGTH: 56

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 672

gggctggcaa gccacgtttg gtggcatctg taaggagaca gcgtcaccat cacttg 56

<210> SEQ ID NO 673

<211> LENGTH: 59

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 673

gggctggcaa gccacgtttg gtggtctgca tctgtaggag acagagtcag tatcatttg 59

<210> SEQ ID NO 674

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 674

gggctggcaa gccacgtttg gtgggagagc cggcctccat ctcctg 46

<210> SEQ ID NO 675

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 675

gggctggcaa gccacgtttg gtgcctggag agccagcctc catctcctg 49

<210> SEQ ID NO 676

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 676

gggctggcaa gccacgtttg gtgctggaga gccggcctcc atctcttg 48

<210> SEQ ID NO 677

<211> LENGTH: 54

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 677

gggctggcaa gccacgtttg gtgtcttcct tggagagcca tcctccattt cctg 54

<210> SEQ ID NO 678

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGKV-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 678

gggctggcaa gccacgtttg gtgggacagc cggcctccat ctcctg 46

<210> SEQ ID NO 679

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 679

gggctggcaa gccacgtttg gtgtggagag ccggcctcca tctcctg 47

<210> SEQ ID NO 680

<211> LENGTH: 59

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGKV-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 680

gggctggcaa gccacgtttg gtgataatat ttgtacataa ctttgtactt catctcctg 59

<210> SEQ ID NO 681

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 681

gggctggcaa gccacgtttg gtgcccctgg aaagccagcc tctatctcct g 51

<210> SEQ ID NO 682

<211> LENGTH: 55

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 682

gggctggcaa gccacgtttg gtgctcttcc ttggagagcc atcctccatt tcctg 55

<210> SEQ ID NO 683

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 683

gggctggcaa gccacgtttg gtgggacagc cggcctccat ctcctt 46

<210> SEQ ID NO 684

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 684

gggctggcaa gccacgtttg gtgcctggag agcaggcctc catgtcctg 49

<210> SEQ ID NO 685

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 685

gggctggcaa gccacgtttg gtgccagggg aaagagccac cctctcctg 49

<210> SEQ ID NO 686

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 686

gggctggcaa gccacgtttg gtgtccaggg gaaagagtca ccctctcctg 50

<210> SEQ ID NO 687

<211> LENGTH: 58

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 687

gggctggcaa gccacgtttg gtgtctttgt ctctggagaa aaaagccacc ctgacttg 58

<210> SEQ ID NO 688

<211> LENGTH: 52

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 688

gggctggcaa gccacgtttg gtgtctctag gggaaaaagc caccctcacc ta 52

<210> SEQ ID NO 689

<211> LENGTH: 46

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 689

gggctggcaa gccacgtttg gtgggggaag gagccaccct cacctg 46

<210> SEQ ID NO 690

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 690

gggctggcaa gccacgtttg gtggggcgag agggccacca tcaactg 47

<210> SEQ ID NO 691

<211> LENGTH: 55

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 691

gggctggcaa gccacgtttg gtggcgactc caggagacaa agtcaacatc tcctg 55

<210> SEQ ID NO 692

<211> LENGTH: 57

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 692

gggctggcaa gccacgtttg gtgctgtgac tccaaaggag aaagtcacca tcacctg 57

<210> SEQ ID NO 693

<211> LENGTH: 52

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 693

gggctggcaa gccacgtttg gtgactccag gggagaaagt caccatcacc tg 52

<210> SEQ ID NO 694

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 694

gggctggcaa gccacgtttg gtgcaggaca gagggccacc atcacctg 48

<210> SEQ ID NO 695

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 695

gggctggcaa gccacgtttg gtgcccaggc agagggtcac catctcctg 49

<210> SEQ ID NO 696

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 696

gggctggcaa gccacgtttg gtgccagggc agagggtcac catctcctg 49

<210> SEQ ID NO 697

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 697

gggctggcaa gccacgtttg gtgccgggca gagggtcacc atctcttg 48

<210> SEQ ID NO 698

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 698

gggctggcaa gccacgtttg gtgccccagg acagaaggtc accatctcct g 51

<210> SEQ ID NO 699

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 699

gggctggcaa gccacgtttg gtgccacaag gcagaggctc actgtctcct g 51

<210> SEQ ID NO 700

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 700

gggctggcaa gccacgtttg gtggtctcct ggacagtcag tcaccatctc ctg 53

<210> SEQ ID NO 701

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 701

gggctggcaa gccacgtttg gtggtctcct ggacagtcga tcaccatctc ctg 53

<210> SEQ ID NO 702

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 702

gggctggcaa gccacgtttg gtgtcctgga cagtcggtca ccatctcctg 50

<210> SEQ ID NO 703

<211> LENGTH: 56

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 703

gggctggcaa gccacgtttg gtgctgggac ttggggtaaa cagtcaccat cttctg 56

<210> SEQ ID NO 704

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 704

gggctggcaa gccacgtttg gtgccaggac agacagccag catcacctg 49

<210> SEQ ID NO 705

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 705

gggctggcaa gccacgtttg gtgctttggg acgtacggcc aggatcatct g 51

<210> SEQ ID NO 706

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 706

gggctggcaa gccacgtttg gtgctttggg acagatggcc aggatcacct g 51

<210> SEQ ID NO 707

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 707

gggctggcaa gccacgtttg gtgccaggac aggcagccat gatcacctg 49

<210> SEQ ID NO 708

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 708

gggctggcaa gccacgtttg gtgtgggaca gagggccagg atcaccta 48

<210> SEQ ID NO 709

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 709

gggctggcaa gccacgtttg gtggggacag gcggccagga ttacctg 47

<210> SEQ ID NO 710

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 710

gggctggcaa gccacgtttg gtgccaggac aaacggccag gatcacctg 49

<210> SEQ ID NO 711

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 711

gggctggcaa gccacgtttg gtgcacagca cagatggcca ggatcacctg 50

<210> SEQ ID NO 712

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 712

gggctggcaa gccacgtttg gtgccaggac agacagccag gatcagctg 49

<210> SEQ ID NO 713

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 713

gggctggcaa gccacgtttg gtgccccagg acagatgacc aggatcacct g 51

<210> SEQ ID NO 714

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 714

gggctggcaa gccacgtttg gtgccctagg acagatggcc aggatcacct g 51

<210> SEQ ID NO 715

<211> LENGTH: 54

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 715

gggctggcaa gccacgtttg gtggtgtctg tggacagtca gcaagggtaa cctg 54

<210> SEQ ID NO 716

<211> LENGTH: 53

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 716

gggctggcaa gccacgtttg gtgggccttg ggacagacag tcaggatcac atg 53

<210> SEQ ID NO 717

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 717

gggctggcaa gccacgtttg gtgccccagg aaagacggcc aggattacct g 51

<210> SEQ ID NO 718

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 718

gggctggcaa gccacgtttg gtgcccagga cagaaagcca ggatcacctg 50

<210> SEQ ID NO 719

<211> LENGTH: 57

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 719

gggctggcaa gccacgtttg gtgcagtagc tccaggacag atgactagga tcacctg 57

<210> SEQ ID NO 720

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 720

gggctggcaa gccacgtttg gtgcaggaca gacggccagg atcacctg 48

<210> SEQ ID NO 721

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 721

gggctggcaa gccacgtttg gtgcctggga cagtcagcca gggtaacctg 50

<210> SEQ ID NO 722

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 722

gggctggcaa gccacgtttg gtgcgggaca gacagccagg atcacctg 48

<210> SEQ ID NO 723

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 723

gggctggcaa gccacgtttg gtgcccagga cagacaccca ggatcacctg 50

<210> SEQ ID NO 724

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 724

gggctggcaa gccacgtttg gtgccccatt acagatggcc aggatcacct g 51

<210> SEQ ID NO 725

<211> LENGTH: 52

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 725

gggctggcaa gccacgtttg gtggccttgg gatagacagc caggatcacc tg 52

<210> SEQ ID NO 726

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 726

gggctggcaa gccacgtttg gtgccttggg acaaatggcc aggatcacct g 51

<210> SEQ ID NO 727

<211> LENGTH: 49

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 727

gggctggcaa gccacgtttg gtgctgggag cctcgatcaa gctcacctg 49

<210> SEQ ID NO 728

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 728

gggctggcaa gccacgtttg gtgcctggga tcctcggtca agctcacctg 50

<210> SEQ ID NO 729

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 729

gggctggcaa gccacgtttg gtggggagcc tcggtcaagc tcacctg 47

<210> SEQ ID NO 730

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 730

gggctggcaa gccacgtttg gtgtcctgga gaatccgcca gactcacctg 50

<210> SEQ ID NO 731

<211> LENGTH: 52

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 731

gggctggcaa gccacgtttg gtgtctcctg gagcatcagc cagattcacc tg 52

<210> SEQ ID NO 732

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 732

gggctggcaa gccacgtttg gtgtcctgga gcatcagcca gtctcacctg 50

<210> SEQ ID NO 733

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 733

gggctggcaa gccacgtttg gtgtcctgga gcatcagcca gactcacctg 50

<210> SEQ ID NO 734

<211> LENGTH: 55

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 734

gggctggcaa gccacgtttg gtggcatctt ctggagcatc agtcagactc acctg 55

<210> SEQ ID NO 735

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 735

gggctggcaa gccacgtttg gtgcccagga gggacagtca ctctcaccta 50

<210> SEQ ID NO 736

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 736

gggctggcaa gccacgtttg gtgcccagga gggacagtca ctctcacctg 50

<210> SEQ ID NO 737

<211> LENGTH: 51

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 737

gggctggcaa gccacgtttg gtgcccctgg agggacagtc acactcactt g 51

<210> SEQ ID NO 738

<211> LENGTH: 48

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 738

gggctggcaa gccacgtttg gtgtgggagc ctcggtcaca ctcacctg 48

<210> SEQ ID NO 739

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 739

gggctggcaa gccacgtttg gtgcttgaga cagaccgcca cactcacctg 50

<210> SEQ ID NO 740

<211> LENGTH: 60

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK V-segment Forward Primer with Universal Adaptor

<400> SEQUENCE: 740

gggctggcaa gccacgtttg gtggtaaata attgcatttt ttaatgaccg tgggtctgtg 60

<210> SEQ ID NO 741

<211> LENGTH: 32

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK J-Segment Reverse Primers

<400> SEQUENCE: 741

ttctactcac gtttgatttc caccttggtc cc 32

<210> SEQ ID NO 742

<211> LENGTH: 32

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK J-Segment Reverse Primers

<400> SEQUENCE: 742

aagtacttac gtttgatctc cagcttggtc cc 32

<210> SEQ ID NO 743

<211> LENGTH: 36

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK J-Segment Reverse Primers

<400> SEQUENCE: 743

acagatgtac ttacgtttga tatccacttt ggtccc 36

<210> SEQ ID NO 744

<211> LENGTH: 29

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK J-Segment Reverse Primers

<400> SEQUENCE: 744

cacttacgtt tgatctccac cttggtccc 29

<210> SEQ ID NO 745

<211> LENGTH: 36

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK J-Segment Reverse Primers

<400> SEQUENCE: 745

gaaaaattac ttacgtttaa tctccagtcg tgtccc 36

<210> SEQ ID NO 746

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL J-Segment Reverse Primers

<400> SEQUENCE: 746

cttacctagg acggtgacct tggtccc 27

<210> SEQ ID NO 747

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL J-Segment Reverse Primers

<400> SEQUENCE: 747

acctaggacg gtcagcttgg tccc 24

<210> SEQ ID NO 748

<211> LENGTH: 36

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL J-Segment Reverse Primers

<400> SEQUENCE: 748

aagaagagac tcatctaaaa tgatcagctg ggttcc 36

<210> SEQ ID NO 749

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL J-Segment Reverse Primers

<400> SEQUENCE: 749

atctaggacg gtcagctccg tccc 24

<210> SEQ ID NO 750

<211> LENGTH: 21

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL J-Segment Reverse Primers

<400> SEQUENCE: 750

gaggacggtc accttggtgc c 21

<210> SEQ ID NO 751

<211> LENGTH: 20

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL J-Segment Reverse Primers

<400> SEQUENCE: 751

aggacggtca gctgggtgcc 20

<210> SEQ ID NO 752

<211> LENGTH: 24

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL J-Segment Reverse Primers

<400> SEQUENCE: 752

ctgcagactc atgaggagtc gccc 24

<210> SEQ ID NO 753

<211> LENGTH: 55

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK J-Segment Reverse Primer

<400> SEQUENCE: 753

ccgggagctg catgtgtcag aggttctact cacgtttgat ttccaccttg gtccc 55

<210> SEQ ID NO 754

<211> LENGTH: 55

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK J-Segment Reverse Primer

<400> SEQUENCE: 754

ccgggagctg catgtgtcag aggaagtact tacgtttgat ctccagcttg gtccc 55

<210> SEQ ID NO 755

<211> LENGTH: 59

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK J-Segment Reverse Primer

<400> SEQUENCE: 755

ccgggagctg catgtgtcag aggacagatg tacttacgtt tgatatccac tttggtccc 59

<210> SEQ ID NO 756

<211> LENGTH: 52

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK J-Segment Reverse Primer

<400> SEQUENCE: 756

ccgggagctg catgtgtcag aggcacttac gtttgatctc caccttggtc cc 52

<210> SEQ ID NO 757

<211> LENGTH: 59

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGK J-Segment Reverse Primer

<400> SEQUENCE: 757

ccgggagctg catgtgtcag agggaaaaat tacttacgtt taatctccag tcgtgtccc 59

<210> SEQ ID NO 758

<211> LENGTH: 50

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL J-Segment Reverse Primer

<400> SEQUENCE: 758

ccgggagctg catgtgtcag aggcttacct aggacggtga ccttggtccc 50

<210> SEQ ID NO 759

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL J-Segment Reverse Primer

<400> SEQUENCE: 759

ccgggagctg catgtgtcag aggacctagg acggtcagct tggtccc 47

<210> SEQ ID NO 760

<211> LENGTH: 59

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL J-Segment Reverse Primer

<400> SEQUENCE: 760

ccgggagctg catgtgtcag aggaagaaga gactcatcta aaatgatcag ctgggttcc 59

<210> SEQ ID NO 761

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL J-Segment Reverse Primer

<400> SEQUENCE: 761

ccgggagctg catgtgtcag aggatctagg acggtcagct ccgtccc 47

<210> SEQ ID NO 762

<211> LENGTH: 44

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL J-Segment Reverse Primer

<400> SEQUENCE: 762

ccgggagctg catgtgtcag agggaggacg gtcaccttgg tgcc 44

<210> SEQ ID NO 763

<211> LENGTH: 43

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL J-Segment Reverse Primer

<400> SEQUENCE: 763

ccgggagctg catgtgtcag aggaggacgg tcagctgggt gcc 43

<210> SEQ ID NO 764

<211> LENGTH: 47

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: IGL J-Segment Reverse Primer

<400> SEQUENCE: 764

ccgggagctg catgtgtcag aggctgcaga ctcatgagga gtcgccc 47

<210> SEQ ID NO 765

<211> LENGTH: 18

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Exemplary Adaptor Sequence

<400> SEQUENCE: 765

aatacgactc actatagg 18

<210> SEQ ID NO 766

<211> LENGTH: 19

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Exemplary Adaptor Sequence

<400> SEQUENCE: 766

gctagttatt gctcagcgg 19

<210> SEQ ID NO 767

<211> LENGTH: 18

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Exemplary Adaptor Sequence

<400> SEQUENCE: 767

attaaccctc actaaagg 18

<210> SEQ ID NO 768

<211> LENGTH: 19

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Exemplary Adaptor Sequence

<400> SEQUENCE: 768

gatttaggtg acactatag 19

<210> SEQ ID NO 769

<211> LENGTH: 18

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Exemplary Adaptor Sequence

<400> SEQUENCE: 769

tgtaaaacga cggccagt 18

<210> SEQ ID NO 770

<211> LENGTH: 17

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Exemplary Adaptor Sequence

<400> SEQUENCE: 770

gttttcccag tcacgac 17

<210> SEQ ID NO 771

<211> LENGTH: 18

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Exemplary Adaptor Sequence

<400> SEQUENCE: 771

caggaaacag ctatgacc 18

<210> SEQ ID NO 772

<211> LENGTH: 21

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Exemplary Adaptor Sequence

<400> SEQUENCE: 772

gactggttcc aattgacaag c 21

<210> SEQ ID NO 773

<211> LENGTH: 21

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Exemplary Adaptor Sequence

<400> SEQUENCE: 773

gcaaatggca ttctgacatc c 21

<210> SEQ ID NO 774

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Exemplary Adaptor Sequence

<400> SEQUENCE: 774

gggctggcaa gccacgtttg gtg 23

<210> SEQ ID NO 775

<211> LENGTH: 23

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Exemplary Adaptor Sequence

<400> SEQUENCE: 775

ccgggagctg catgtgtcag agg 23

<210> SEQ ID NO 776

<211> LENGTH: 22

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Exemplary Adaptor Sequence

<400> SEQUENCE: 776

aactagaagg cacagtcgag gc 22

<210> SEQ ID NO 777

<211> LENGTH: 20

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Exemplary Adaptor Sequence

<400> SEQUENCE: 777

cactctcggc atggacgagc 20

<210> SEQ ID NO 778

<211> LENGTH: 20

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Exemplary Adaptor Sequence

<400> SEQUENCE: 778

tggtgcagat gaacttcagg 20

<210> SEQ ID NO 779

<211> LENGTH: 20

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Exemplary Adaptor Sequence

<400> SEQUENCE: 779

gttcgacccc gcctcgatcc 20

<210> SEQ ID NO 780

<211> LENGTH: 21

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Exemplary Adaptor Sequence

<400> SEQUENCE: 780

tgacacacat tccacagggt c 21

<210> SEQ ID NO 781

<211> LENGTH: 19

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Exemplary Adaptor Sequence

<400> SEQUENCE: 781

gcgtgaatgt aagcgtgac 19

<210> SEQ ID NO 782

<211> LENGTH: 20

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Exemplary Adaptor Sequence

<400> SEQUENCE: 782

ggattattca taccgtccca 20

<210> SEQ ID NO 783

<211> LENGTH: 20

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Exemplary Adaptor Sequence

<400> SEQUENCE: 783

caaatgtggt atggctgatt 20

<210> SEQ ID NO 784

<211> LENGTH: 20

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Exemplary Adaptor Sequence

<400> SEQUENCE: 784

atgccatagc atttttatcc 20

<210> SEQ ID NO 785

<211> LENGTH: 18

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Exemplary Adaptor Sequence

<400> SEQUENCE: 785

gatttaatct gtatcagg 18

<210> SEQ ID NO 786

<211> LENGTH: 21

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Exemplary Adaptor Sequence

<400> SEQUENCE: 786

cgcaaatggg cggtaggcgt g 21

<210> SEQ ID NO 787

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 787

gccttgccag cccgctcaga cacacgtgac actcttctta ttttcatagg ctccatggat 60

actggaatta cccagacacc aaaatacctg gtcacagcaa tggggagtaa aaggacaatg 120

aaacgtgagc atctgggaca tgattctatg tattggtaca gacagaaagc taagaaatcc 180

ctggagttca tgttttacta caactgtaag gaattcattg aaaacaagac tgtgccaaat 240

cacttcacac ctgaatgccc tgacagctct cgcttatacc ttcatgtggt cgcactgcag 300

caagaagact cagctgcgta tctctgcacc agcagtgaac acacgtgaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag acacacgtga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 788

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 788

gccttgccag cccgctcaga cacacgtttc ggaactctta ttttcatagg ctccatggat 60

actggaatta cccagacacc aaaatacctg gtcacagcaa tggggagtaa aaggacaatg 120

aaacgtgagc atctgggaca tgattctatg tattggtaca gacagaaagc taagaaatcc 180

ctggagttca tgttttacta caactgtaag gaattcattg aaaacaagac tgtgccaaat 240

cacttcacac ctgaatgccc tgacagctct cgcttatacc ttcatgtggt cgcactgcag 300

caagaagact cagctgcgta tctctgcacc agcagtgaac acacgtttcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat acacacgttt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 789

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 789

gccttgccag cccgctcaga cacacgtaag taacgtctta ttttcatagg ctccatggat 60

actggaatta cccagacacc aaaatacctg gtcacagcaa tggggagtaa aaggacaatg 120

aaacgtgagc atctgggaca tgattctatg tattggtaca gacagaaagc taagaaatcc 180

ctggagttca tgttttacta caactgtaag gaattcattg aaaacaagac tgtgccaaat 240

cacttcacac ctgaatgccc tgacagctct cgcttatacc ttcatgtggt cgcactgcag 300

caagaagact cagctgcgta tctctgcacc agcagtgaac acacgtaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag acacacgtaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 790

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 790

gccttgccag cccgctcaga cacacgtgtc tcctatctta ttttcatagg ctccatggat 60

actggaatta cccagacacc aaaatacctg gtcacagcaa tggggagtaa aaggacaatg 120

aaacgtgagc atctgggaca tgattctatg tattggtaca gacagaaagc taagaaatcc 180

ctggagttca tgttttacta caactgtaag gaattcattg aaaacaagac tgtgccaaat 240

cacttcacac ctgaatgccc tgacagctct cgcttatacc ttcatgtggt cgcactgcag 300

caagaagact cagctgcgta tctctgcacc agcagtgaac acacgtgtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag acacacgtgt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 791

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 791

gccttgccag cccgctcaga cacacgtaga gtgtctctta ttttcatagg ctccatggat 60

actggaatta cccagacacc aaaatacctg gtcacagcaa tggggagtaa aaggacaatg 120

aaacgtgagc atctgggaca tgattctatg tattggtaca gacagaaagc taagaaatcc 180

ctggagttca tgttttacta caactgtaag gaattcattg aaaacaagac tgtgccaaat 240

cacttcacac ctgaatgccc tgacagctct cgcttatacc ttcatgtggt cgcactgcag 300

caagaagact cagctgcgta tctctgcacc agcagtgaac acacgtagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct acacacgtag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 792

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 792

gccttgccag cccgctcaga cacacgtgtt ccgaatctta ttttcatagg ctccatggat 60

actggaatta cccagacacc aaaatacctg gtcacagcaa tggggagtaa aaggacaatg 120

aaacgtgagc atctgggaca tgattctatg tattggtaca gacagaaagc taagaaatcc 180

ctggagttca tgttttacta caactgtaag gaattcattg aaaacaagac tgtgccaaat 240

cacttcacac ctgaatgccc tgacagctct cgcttatacc ttcatgtggt cgcactgcag 300

caagaagact cagctgcgta tctctgcacc agcagtgaac acacgtgttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat acacacgtgt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 793

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 793

gccttgccag cccgctcaga cacacgtcgt tactttctta ttttcatagg ctccatggat 60

actggaatta cccagacacc aaaatacctg gtcacagcaa tggggagtaa aaggacaatg 120

aaacgtgagc atctgggaca tgattctatg tattggtaca gacagaaagc taagaaatcc 180

ctggagttca tgttttacta caactgtaag gaattcattg aaaacaagac tgtgccaaat 240

cacttcacac ctgaatgccc tgacagctct cgcttatacc ttcatgtggt cgcactgcag 300

caagaagact cagctgcgta tctctgcacc agcagtgaac acacgtcgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc acacacgtcg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 794

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 794

gccttgccag cccgctcaga cacacgttag gagactctta ttttcatagg ctccatggat 60

actggaatta cccagacacc aaaatacctg gtcacagcaa tggggagtaa aaggacaatg 120

aaacgtgagc atctgggaca tgattctatg tattggtaca gacagaaagc taagaaatcc 180

ctggagttca tgttttacta caactgtaag gaattcattg aaaacaagac tgtgccaaat 240

cacttcacac ctgaatgccc tgacagctct cgcttatacc ttcatgtggt cgcactgcag 300

caagaagact cagctgcgta tctctgcacc agcagtgaac acacgttagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg acacacgtta ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 795

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 795

gccttgccag cccgctcaga cacacgtgtg tctactctta ttttcatagg ctccatggat 60

actggaatta cccagacacc aaaatacctg gtcacagcaa tggggagtaa aaggacaatg 120

aaacgtgagc atctgggaca tgattctatg tattggtaca gacagaaagc taagaaatcc 180

ctggagttca tgttttacta caactgtaag gaattcattg aaaacaagac tgtgccaaat 240

cacttcacac ctgaatgccc tgacagctct cgcttatacc ttcatgtggt cgcactgcag 300

caagaagact cagctgcgta tctctgcacc agcagtgaac acacgtgtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc acacacgtgt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 796

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 796

gccttgccag cccgctcaga cacacgtgta cagtgtctta ttttcatagg ctccatggat 60

actggaatta cccagacacc aaaatacctg gtcacagcaa tggggagtaa aaggacaatg 120

aaacgtgagc atctgggaca tgattctatg tattggtaca gacagaaagc taagaaatcc 180

ctggagttca tgttttacta caactgtaag gaattcattg aaaacaagac tgtgccaaat 240

cacttcacac ctgaatgccc tgacagctct cgcttatacc ttcatgtggt cgcactgcag 300

caagaagact cagctgcgta tctctgcacc agcagtgaac acacgtgtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg acacacgtgt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 797

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 797

gccttgccag cccgctcaga cacacgtgga tcatctctta ttttcatagg ctccatggat 60

actggaatta cccagacacc aaaatacctg gtcacagcaa tggggagtaa aaggacaatg 120

aaacgtgagc atctgggaca tgattctatg tattggtaca gacagaaagc taagaaatcc 180

ctggagttca tgttttacta caactgtaag gaattcattg aaaacaagac tgtgccaaat 240

cacttcacac ctgaatgccc tgacagctct cgcttatacc ttcatgtggt cgcactgcag 300

caagaagact cagctgcgta tctctgcacc agcagtgaac acacgtggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg acacacgtgg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 798

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 798

gccttgccag cccgctcaga cacacgttat tggcgtctta ttttcatagg ctccatggat 60

actggaatta cccagacacc aaaatacctg gtcacagcaa tggggagtaa aaggacaatg 120

aaacgtgagc atctgggaca tgattctatg tattggtaca gacagaaagc taagaaatcc 180

ctggagttca tgttttacta caactgtaag gaattcattg aaaacaagac tgtgccaaat 240

cacttcacac ctgaatgccc tgacagctct cgcttatacc ttcatgtggt cgcactgcag 300

caagaagact cagctgcgta tctctgcacc agcagtgaac acacgttatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg acacacgtta ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 799

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 799

gccttgccag cccgctcaga cacacgtagg cttgatctta ttttcatagg ctccatggat 60

actggaatta cccagacacc aaaatacctg gtcacagcaa tggggagtaa aaggacaatg 120

aaacgtgagc atctgggaca tgattctatg tattggtaca gacagaaagc taagaaatcc 180

ctggagttca tgttttacta caactgtaag gaattcattg aaaacaagac tgtgccaaat 240

cacttcacac ctgaatgccc tgacagctct cgcttatacc ttcatgtggt cgcactgcag 300

caagaagact cagctgcgta tctctgcacc agcagtgaac acacgtaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg acacacgtag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 800

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 800

gccttgccag cccgctcagt agacggagac actcttctca caggactcac agaacctgaa 60

gtcacccaga ctcccagcca tcaggtcaca cagatgggac aggaagtgat cttgcgctgt 120

gtccccatct ctaatcactt atacttctat tggtacagac aaatcttggg gcagaaagtc 180

gagtttctgg tttcctttta taataatgaa atctcagaga agtctgaaat attcgatgat 240

caattctcag ttgaaaggcc tgatggatca aatttcactc tgaagatccg gtccacaaag 300

ctggaggact cagccatgta cttctgtgcc agcagtgata gacggagaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag tagacggaga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 801

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 801

gccttgccag cccgctcagt agacggattc ggaactctca caggactcac agaacctgaa 60

gtcacccaga ctcccagcca tcaggtcaca cagatgggac aggaagtgat cttgcgctgt 120

gtccccatct ctaatcactt atacttctat tggtacagac aaatcttggg gcagaaagtc 180

gagtttctgg tttcctttta taataatgaa atctcagaga agtctgaaat attcgatgat 240

caattctcag ttgaaaggcc tgatggatca aatttcactc tgaagatccg gtccacaaag 300

ctggaggact cagccatgta cttctgtgcc agcagtgata gacggattcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat tagacggatt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 802

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 802

gccttgccag cccgctcagt agacggaaag taacgtctca caggactcac agaacctgaa 60

gtcacccaga ctcccagcca tcaggtcaca cagatgggac aggaagtgat cttgcgctgt 120

gtccccatct ctaatcactt atacttctat tggtacagac aaatcttggg gcagaaagtc 180

gagtttctgg tttcctttta taataatgaa atctcagaga agtctgaaat attcgatgat 240

caattctcag ttgaaaggcc tgatggatca aatttcactc tgaagatccg gtccacaaag 300

ctggaggact cagccatgta cttctgtgcc agcagtgata gacggaaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag tagacggaaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 803

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 803

gccttgccag cccgctcagt agacggagtc tcctatctca caggactcac agaacctgaa 60

gtcacccaga ctcccagcca tcaggtcaca cagatgggac aggaagtgat cttgcgctgt 120

gtccccatct ctaatcactt atacttctat tggtacagac aaatcttggg gcagaaagtc 180

gagtttctgg tttcctttta taataatgaa atctcagaga agtctgaaat attcgatgat 240

caattctcag ttgaaaggcc tgatggatca aatttcactc tgaagatccg gtccacaaag 300

ctggaggact cagccatgta cttctgtgcc agcagtgata gacggagtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag tagacggagt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 804

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 804

gccttgccag cccgctcagt agacggaaga gtgtctctca caggactcac agaacctgaa 60

gtcacccaga ctcccagcca tcaggtcaca cagatgggac aggaagtgat cttgcgctgt 120

gtccccatct ctaatcactt atacttctat tggtacagac aaatcttggg gcagaaagtc 180

gagtttctgg tttcctttta taataatgaa atctcagaga agtctgaaat attcgatgat 240

caattctcag ttgaaaggcc tgatggatca aatttcactc tgaagatccg gtccacaaag 300

ctggaggact cagccatgta cttctgtgcc agcagtgata gacggaagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct tagacggaag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 805

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 805

gccttgccag cccgctcagt agacggagtt ccgaatctca caggactcac agaacctgaa 60

gtcacccaga ctcccagcca tcaggtcaca cagatgggac aggaagtgat cttgcgctgt 120

gtccccatct ctaatcactt atacttctat tggtacagac aaatcttggg gcagaaagtc 180

gagtttctgg tttcctttta taataatgaa atctcagaga agtctgaaat attcgatgat 240

caattctcag ttgaaaggcc tgatggatca aatttcactc tgaagatccg gtccacaaag 300

ctggaggact cagccatgta cttctgtgcc agcagtgata gacggagttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat tagacggagt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 806

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 806

gccttgccag cccgctcagt agacggacgt tactttctca caggactcac agaacctgaa 60

gtcacccaga ctcccagcca tcaggtcaca cagatgggac aggaagtgat cttgcgctgt 120

gtccccatct ctaatcactt atacttctat tggtacagac aaatcttggg gcagaaagtc 180

gagtttctgg tttcctttta taataatgaa atctcagaga agtctgaaat attcgatgat 240

caattctcag ttgaaaggcc tgatggatca aatttcactc tgaagatccg gtccacaaag 300

ctggaggact cagccatgta cttctgtgcc agcagtgata gacggacgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc tagacggacg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 807

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 807

gccttgccag cccgctcagt agacggatag gagactctca caggactcac agaacctgaa 60

gtcacccaga ctcccagcca tcaggtcaca cagatgggac aggaagtgat cttgcgctgt 120

gtccccatct ctaatcactt atacttctat tggtacagac aaatcttggg gcagaaagtc 180

gagtttctgg tttcctttta taataatgaa atctcagaga agtctgaaat attcgatgat 240

caattctcag ttgaaaggcc tgatggatca aatttcactc tgaagatccg gtccacaaag 300

ctggaggact cagccatgta cttctgtgcc agcagtgata gacggatagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg tagacggata ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 808

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 808

gccttgccag cccgctcagt agacggagtg tctactctca caggactcac agaacctgaa 60

gtcacccaga ctcccagcca tcaggtcaca cagatgggac aggaagtgat cttgcgctgt 120

gtccccatct ctaatcactt atacttctat tggtacagac aaatcttggg gcagaaagtc 180

gagtttctgg tttcctttta taataatgaa atctcagaga agtctgaaat attcgatgat 240

caattctcag ttgaaaggcc tgatggatca aatttcactc tgaagatccg gtccacaaag 300

ctggaggact cagccatgta cttctgtgcc agcagtgata gacggagtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc tagacggagt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 809

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 809

gccttgccag cccgctcagt agacggagta cagtgtctca caggactcac agaacctgaa 60

gtcacccaga ctcccagcca tcaggtcaca cagatgggac aggaagtgat cttgcgctgt 120

gtccccatct ctaatcactt atacttctat tggtacagac aaatcttggg gcagaaagtc 180

gagtttctgg tttcctttta taataatgaa atctcagaga agtctgaaat attcgatgat 240

caattctcag ttgaaaggcc tgatggatca aatttcactc tgaagatccg gtccacaaag 300

ctggaggact cagccatgta cttctgtgcc agcagtgata gacggagtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg tagacggagt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 810

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 810

gccttgccag cccgctcagt agacggagga tcatctctca caggactcac agaacctgaa 60

gtcacccaga ctcccagcca tcaggtcaca cagatgggac aggaagtgat cttgcgctgt 120

gtccccatct ctaatcactt atacttctat tggtacagac aaatcttggg gcagaaagtc 180

gagtttctgg tttcctttta taataatgaa atctcagaga agtctgaaat attcgatgat 240

caattctcag ttgaaaggcc tgatggatca aatttcactc tgaagatccg gtccacaaag 300

ctggaggact cagccatgta cttctgtgcc agcagtgata gacggaggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg tagacggagg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 811

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 811

gccttgccag cccgctcagt agacggatat tggcgtctca caggactcac agaacctgaa 60

gtcacccaga ctcccagcca tcaggtcaca cagatgggac aggaagtgat cttgcgctgt 120

gtccccatct ctaatcactt atacttctat tggtacagac aaatcttggg gcagaaagtc 180

gagtttctgg tttcctttta taataatgaa atctcagaga agtctgaaat attcgatgat 240

caattctcag ttgaaaggcc tgatggatca aatttcactc tgaagatccg gtccacaaag 300

ctggaggact cagccatgta cttctgtgcc agcagtgata gacggatatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg tagacggata ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 812

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 812

gccttgccag cccgctcagt agacggaagg cttgatctca caggactcac agaacctgaa 60

gtcacccaga ctcccagcca tcaggtcaca cagatgggac aggaagtgat cttgcgctgt 120

gtccccatct ctaatcactt atacttctat tggtacagac aaatcttggg gcagaaagtc 180

gagtttctgg tttcctttta taataatgaa atctcagaga agtctgaaat attcgatgat 240

caattctcag ttgaaaggcc tgatggatca aatttcactc tgaagatccg gtccacaaag 300

ctggaggact cagccatgta cttctgtgcc agcagtgata gacggaaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg tagacggaag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 813

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 813

gccttgccag cccgctcagc agctcttgac actctttcct ttgcaggtcc cttggacaca 60

gctgtttccc agactccaaa atacctggtc acacagatgg gaaacgacaa gtccattaaa 120

tgtgaacaaa atctgggcca tgatactatg tattggtata aacaggactc taagaaattt 180

ctgaagataa tgtttagcta caataataag gagctcatta taaatgaaac agttccaaat 240

cgcttctcac ctaaatctcc agacaaagct cacttaaatc ttcacatcaa ttccctggag 300

cttggtgact ctgctgtgta tttctgtgcc agcagtgaca gctcttgaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag cagctcttga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 814

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 814

gccttgccag cccgctcagc agctcttttc ggaacttcct ttgcaggtcc cttggacaca 60

gctgtttccc agactccaaa atacctggtc acacagatgg gaaacgacaa gtccattaaa 120

tgtgaacaaa atctgggcca tgatactatg tattggtata aacaggactc taagaaattt 180

ctgaagataa tgtttagcta caataataag gagctcatta taaatgaaac agttccaaat 240

cgcttctcac ctaaatctcc agacaaagct cacttaaatc ttcacatcaa ttccctggag 300

cttggtgact ctgctgtgta tttctgtgcc agcagtgaca gctcttttcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat cagctctttt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 815

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 815

gccttgccag cccgctcagc agctcttaag taacgttcct ttgcaggtcc cttggacaca 60

gctgtttccc agactccaaa atacctggtc acacagatgg gaaacgacaa gtccattaaa 120

tgtgaacaaa atctgggcca tgatactatg tattggtata aacaggactc taagaaattt 180

ctgaagataa tgtttagcta caataataag gagctcatta taaatgaaac agttccaaat 240

cgcttctcac ctaaatctcc agacaaagct cacttaaatc ttcacatcaa ttccctggag 300

cttggtgact ctgctgtgta tttctgtgcc agcagtgaca gctcttaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag cagctcttaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 816

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 816

gccttgccag cccgctcagc agctcttgtc tcctattcct ttgcaggtcc cttggacaca 60

gctgtttccc agactccaaa atacctggtc acacagatgg gaaacgacaa gtccattaaa 120

tgtgaacaaa atctgggcca tgatactatg tattggtata aacaggactc taagaaattt 180

ctgaagataa tgtttagcta caataataag gagctcatta taaatgaaac agttccaaat 240

cgcttctcac ctaaatctcc agacaaagct cacttaaatc ttcacatcaa ttccctggag 300

cttggtgact ctgctgtgta tttctgtgcc agcagtgaca gctcttgtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag cagctcttgt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 817

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 817

gccttgccag cccgctcagc agctcttaga gtgtcttcct ttgcaggtcc cttggacaca 60

gctgtttccc agactccaaa atacctggtc acacagatgg gaaacgacaa gtccattaaa 120

tgtgaacaaa atctgggcca tgatactatg tattggtata aacaggactc taagaaattt 180

ctgaagataa tgtttagcta caataataag gagctcatta taaatgaaac agttccaaat 240

cgcttctcac ctaaatctcc agacaaagct cacttaaatc ttcacatcaa ttccctggag 300

cttggtgact ctgctgtgta tttctgtgcc agcagtgaca gctcttagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct cagctcttag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 818

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 818

gccttgccag cccgctcagc agctcttgtt ccgaattcct ttgcaggtcc cttggacaca 60

gctgtttccc agactccaaa atacctggtc acacagatgg gaaacgacaa gtccattaaa 120

tgtgaacaaa atctgggcca tgatactatg tattggtata aacaggactc taagaaattt 180

ctgaagataa tgtttagcta caataataag gagctcatta taaatgaaac agttccaaat 240

cgcttctcac ctaaatctcc agacaaagct cacttaaatc ttcacatcaa ttccctggag 300

cttggtgact ctgctgtgta tttctgtgcc agcagtgaca gctcttgttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat cagctcttgt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 819

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 819

gccttgccag cccgctcagc agctcttcgt tacttttcct ttgcaggtcc cttggacaca 60

gctgtttccc agactccaaa atacctggtc acacagatgg gaaacgacaa gtccattaaa 120

tgtgaacaaa atctgggcca tgatactatg tattggtata aacaggactc taagaaattt 180

ctgaagataa tgtttagcta caataataag gagctcatta taaatgaaac agttccaaat 240

cgcttctcac ctaaatctcc agacaaagct cacttaaatc ttcacatcaa ttccctggag 300

cttggtgact ctgctgtgta tttctgtgcc agcagtgaca gctcttcgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc cagctcttcg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 820

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 820

gccttgccag cccgctcagc agctctttag gagacttcct ttgcaggtcc cttggacaca 60

gctgtttccc agactccaaa atacctggtc acacagatgg gaaacgacaa gtccattaaa 120

tgtgaacaaa atctgggcca tgatactatg tattggtata aacaggactc taagaaattt 180

ctgaagataa tgtttagcta caataataag gagctcatta taaatgaaac agttccaaat 240

cgcttctcac ctaaatctcc agacaaagct cacttaaatc ttcacatcaa ttccctggag 300

cttggtgact ctgctgtgta tttctgtgcc agcagtgaca gctctttagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg cagctcttta ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 821

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 821

gccttgccag cccgctcagc agctcttgtg tctacttcct ttgcaggtcc cttggacaca 60

gctgtttccc agactccaaa atacctggtc acacagatgg gaaacgacaa gtccattaaa 120

tgtgaacaaa atctgggcca tgatactatg tattggtata aacaggactc taagaaattt 180

ctgaagataa tgtttagcta caataataag gagctcatta taaatgaaac agttccaaat 240

cgcttctcac ctaaatctcc agacaaagct cacttaaatc ttcacatcaa ttccctggag 300

cttggtgact ctgctgtgta tttctgtgcc agcagtgaca gctcttgtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc cagctcttgt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 822

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 822

gccttgccag cccgctcagc agctcttgta cagtgttcct ttgcaggtcc cttggacaca 60

gctgtttccc agactccaaa atacctggtc acacagatgg gaaacgacaa gtccattaaa 120

tgtgaacaaa atctgggcca tgatactatg tattggtata aacaggactc taagaaattt 180

ctgaagataa tgtttagcta caataataag gagctcatta taaatgaaac agttccaaat 240

cgcttctcac ctaaatctcc agacaaagct cacttaaatc ttcacatcaa ttccctggag 300

cttggtgact ctgctgtgta tttctgtgcc agcagtgaca gctcttgtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg cagctcttgt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 823

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 823

gccttgccag cccgctcagc agctcttgga tcatcttcct ttgcaggtcc cttggacaca 60

gctgtttccc agactccaaa atacctggtc acacagatgg gaaacgacaa gtccattaaa 120

tgtgaacaaa atctgggcca tgatactatg tattggtata aacaggactc taagaaattt 180

ctgaagataa tgtttagcta caataataag gagctcatta taaatgaaac agttccaaat 240

cgcttctcac ctaaatctcc agacaaagct cacttaaatc ttcacatcaa ttccctggag 300

cttggtgact ctgctgtgta tttctgtgcc agcagtgaca gctcttggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg cagctcttgg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 824

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 824

gccttgccag cccgctcagc agctctttat tggcgttcct ttgcaggtcc cttggacaca 60

gctgtttccc agactccaaa atacctggtc acacagatgg gaaacgacaa gtccattaaa 120

tgtgaacaaa atctgggcca tgatactatg tattggtata aacaggactc taagaaattt 180

ctgaagataa tgtttagcta caataataag gagctcatta taaatgaaac agttccaaat 240

cgcttctcac ctaaatctcc agacaaagct cacttaaatc ttcacatcaa ttccctggag 300

cttggtgact ctgctgtgta tttctgtgcc agcagtgaca gctctttatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg cagctcttta ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 825

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 825

gccttgccag cccgctcagc agctcttagg cttgattcct ttgcaggtcc cttggacaca 60

gctgtttccc agactccaaa atacctggtc acacagatgg gaaacgacaa gtccattaaa 120

tgtgaacaaa atctgggcca tgatactatg tattggtata aacaggactc taagaaattt 180

ctgaagataa tgtttagcta caataataag gagctcatta taaatgaaac agttccaaat 240

cgcttctcac ctaaatctcc agacaaagct cacttaaatc ttcacatcaa ttccctggag 300

cttggtgact ctgctgtgta tttctgtgcc agcagtgaca gctcttaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg cagctcttag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 826

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 826

gccttgccag cccgctcagg agcgatagac actctttcct ttacagatcc actggacaca 60

gccgtttccc agactccaaa atacctggtc acacagatgg gaaaaaagga gtctcttaaa 120

tgagaacaaa atctgggcca taatgctatg tattggtata aacaggactc taagaaattt 180

ctgaagacaa tgtttatcta cagtaacaag gagccaattt taaatgaaac agttccaaat 240

cgcttctcac ctgactctcc agacaaagct catttaaatc ttcacatcaa ttccctggag 300

cttggtgact ctgctgtgta tttctgtgcc agcagtgaga gcgatagaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag gagcgataga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 827

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 827

gccttgccag cccgctcagg agcgatattc ggaacttcct ttacagatcc actggacaca 60

gccgtttccc agactccaaa atacctggtc acacagatgg gaaaaaagga gtctcttaaa 120

tgagaacaaa atctgggcca taatgctatg tattggtata aacaggactc taagaaattt 180

ctgaagacaa tgtttatcta cagtaacaag gagccaattt taaatgaaac agttccaaat 240

cgcttctcac ctgactctcc agacaaagct catttaaatc ttcacatcaa ttccctggag 300

cttggtgact ctgctgtgta tttctgtgcc agcagtgaga gcgatattcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat gagcgatatt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 828

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 828

gccttgccag cccgctcagg agcgataaag taacgttcct ttacagatcc actggacaca 60

gccgtttccc agactccaaa atacctggtc acacagatgg gaaaaaagga gtctcttaaa 120

tgagaacaaa atctgggcca taatgctatg tattggtata aacaggactc taagaaattt 180

ctgaagacaa tgtttatcta cagtaacaag gagccaattt taaatgaaac agttccaaat 240

cgcttctcac ctgactctcc agacaaagct catttaaatc ttcacatcaa ttccctggag 300

cttggtgact ctgctgtgta tttctgtgcc agcagtgaga gcgataaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag gagcgataaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 829

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 829

gccttgccag cccgctcagg agcgatagtc tcctattcct ttacagatcc actggacaca 60

gccgtttccc agactccaaa atacctggtc acacagatgg gaaaaaagga gtctcttaaa 120

tgagaacaaa atctgggcca taatgctatg tattggtata aacaggactc taagaaattt 180

ctgaagacaa tgtttatcta cagtaacaag gagccaattt taaatgaaac agttccaaat 240

cgcttctcac ctgactctcc agacaaagct catttaaatc ttcacatcaa ttccctggag 300

cttggtgact ctgctgtgta tttctgtgcc agcagtgaga gcgatagtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag gagcgatagt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 830

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 830

gccttgccag cccgctcagg agcgataaga gtgtcttcct ttacagatcc actggacaca 60

gccgtttccc agactccaaa atacctggtc acacagatgg gaaaaaagga gtctcttaaa 120

tgagaacaaa atctgggcca taatgctatg tattggtata aacaggactc taagaaattt 180

ctgaagacaa tgtttatcta cagtaacaag gagccaattt taaatgaaac agttccaaat 240

cgcttctcac ctgactctcc agacaaagct catttaaatc ttcacatcaa ttccctggag 300

cttggtgact ctgctgtgta tttctgtgcc agcagtgaga gcgataagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct gagcgataag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 831

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 831

gccttgccag cccgctcagg agcgatagtt ccgaattcct ttacagatcc actggacaca 60

gccgtttccc agactccaaa atacctggtc acacagatgg gaaaaaagga gtctcttaaa 120

tgagaacaaa atctgggcca taatgctatg tattggtata aacaggactc taagaaattt 180

ctgaagacaa tgtttatcta cagtaacaag gagccaattt taaatgaaac agttccaaat 240

cgcttctcac ctgactctcc agacaaagct catttaaatc ttcacatcaa ttccctggag 300

cttggtgact ctgctgtgta tttctgtgcc agcagtgaga gcgatagttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat gagcgatagt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 832

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 832

gccttgccag cccgctcagg agcgatacgt tacttttcct ttacagatcc actggacaca 60

gccgtttccc agactccaaa atacctggtc acacagatgg gaaaaaagga gtctcttaaa 120

tgagaacaaa atctgggcca taatgctatg tattggtata aacaggactc taagaaattt 180

ctgaagacaa tgtttatcta cagtaacaag gagccaattt taaatgaaac agttccaaat 240

cgcttctcac ctgactctcc agacaaagct catttaaatc ttcacatcaa ttccctggag 300

cttggtgact ctgctgtgta tttctgtgcc agcagtgaga gcgatacgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc gagcgatacg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 833

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 833

gccttgccag cccgctcagg agcgatatag gagacttcct ttacagatcc actggacaca 60

gccgtttccc agactccaaa atacctggtc acacagatgg gaaaaaagga gtctcttaaa 120

tgagaacaaa atctgggcca taatgctatg tattggtata aacaggactc taagaaattt 180

ctgaagacaa tgtttatcta cagtaacaag gagccaattt taaatgaaac agttccaaat 240

cgcttctcac ctgactctcc agacaaagct catttaaatc ttcacatcaa ttccctggag 300

cttggtgact ctgctgtgta tttctgtgcc agcagtgaga gcgatatagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg gagcgatata ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 834

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 834

gccttgccag cccgctcagg agcgatagtg tctacttcct ttacagatcc actggacaca 60

gccgtttccc agactccaaa atacctggtc acacagatgg gaaaaaagga gtctcttaaa 120

tgagaacaaa atctgggcca taatgctatg tattggtata aacaggactc taagaaattt 180

ctgaagacaa tgtttatcta cagtaacaag gagccaattt taaatgaaac agttccaaat 240

cgcttctcac ctgactctcc agacaaagct catttaaatc ttcacatcaa ttccctggag 300

cttggtgact ctgctgtgta tttctgtgcc agcagtgaga gcgatagtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc gagcgatagt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 835

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 835

gccttgccag cccgctcagg agcgatagta cagtgttcct ttacagatcc actggacaca 60

gccgtttccc agactccaaa atacctggtc acacagatgg gaaaaaagga gtctcttaaa 120

tgagaacaaa atctgggcca taatgctatg tattggtata aacaggactc taagaaattt 180

ctgaagacaa tgtttatcta cagtaacaag gagccaattt taaatgaaac agttccaaat 240

cgcttctcac ctgactctcc agacaaagct catttaaatc ttcacatcaa ttccctggag 300

cttggtgact ctgctgtgta tttctgtgcc agcagtgaga gcgatagtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg gagcgatagt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 836

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 836

gccttgccag cccgctcagg agcgatagga tcatcttcct ttacagatcc actggacaca 60

gccgtttccc agactccaaa atacctggtc acacagatgg gaaaaaagga gtctcttaaa 120

tgagaacaaa atctgggcca taatgctatg tattggtata aacaggactc taagaaattt 180

ctgaagacaa tgtttatcta cagtaacaag gagccaattt taaatgaaac agttccaaat 240

cgcttctcac ctgactctcc agacaaagct catttaaatc ttcacatcaa ttccctggag 300

cttggtgact ctgctgtgta tttctgtgcc agcagtgaga gcgataggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg gagcgatagg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 837

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 837

gccttgccag cccgctcagg agcgatatat tggcgttcct ttacagatcc actggacaca 60

gccgtttccc agactccaaa atacctggtc acacagatgg gaaaaaagga gtctcttaaa 120

tgagaacaaa atctgggcca taatgctatg tattggtata aacaggactc taagaaattt 180

ctgaagacaa tgtttatcta cagtaacaag gagccaattt taaatgaaac agttccaaat 240

cgcttctcac ctgactctcc agacaaagct catttaaatc ttcacatcaa ttccctggag 300

cttggtgact ctgctgtgta tttctgtgcc agcagtgaga gcgatatatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg gagcgatata ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 838

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 838

gccttgccag cccgctcagg agcgataagg cttgattcct ttacagatcc actggacaca 60

gccgtttccc agactccaaa atacctggtc acacagatgg gaaaaaagga gtctcttaaa 120

tgagaacaaa atctgggcca taatgctatg tattggtata aacaggactc taagaaattt 180

ctgaagacaa tgtttatcta cagtaacaag gagccaattt taaatgaaac agttccaaat 240

cgcttctcac ctgactctcc agacaaagct catttaaatc ttcacatcaa ttccctggag 300

cttggtgact ctgctgtgta tttctgtgcc agcagtgaga gcgataaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg gagcgataag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 839

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 839

gccttgccag cccgctcagg catctgagac actcttcctc ctacagttcc catagacact 60

gaagttaccc agacaccaaa acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atatggggca cagggctatg tattggtaca agcagaaagc taagaagcca 180

ccggagctca tgtttgtcta cagctatgag aaactctcta taaatgaaag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct ctcttaaacc ttcacctaca cgccctgcag 300

ccagaagact cagccctgta tctctgcgcc agcagtgagc atctgagaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag gcatctgaga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 840

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 840

gccttgccag cccgctcagg catctgattc ggaactcctc ctacagttcc catagacact 60

gaagttaccc agacaccaaa acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atatggggca cagggctatg tattggtaca agcagaaagc taagaagcca 180

ccggagctca tgtttgtcta cagctatgag aaactctcta taaatgaaag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct ctcttaaacc ttcacctaca cgccctgcag 300

ccagaagact cagccctgta tctctgcgcc agcagtgagc atctgattcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat gcatctgatt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 841

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 841

gccttgccag cccgctcagg catctgaaag taacgtcctc ctacagttcc catagacact 60

gaagttaccc agacaccaaa acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atatggggca cagggctatg tattggtaca agcagaaagc taagaagcca 180

ccggagctca tgtttgtcta cagctatgag aaactctcta taaatgaaag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct ctcttaaacc ttcacctaca cgccctgcag 300

ccagaagact cagccctgta tctctgcgcc agcagtgagc atctgaaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag gcatctgaaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 842

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 842

gccttgccag cccgctcagg catctgagtc tcctatcctc ctacagttcc catagacact 60

gaagttaccc agacaccaaa acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atatggggca cagggctatg tattggtaca agcagaaagc taagaagcca 180

ccggagctca tgtttgtcta cagctatgag aaactctcta taaatgaaag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct ctcttaaacc ttcacctaca cgccctgcag 300

ccagaagact cagccctgta tctctgcgcc agcagtgagc atctgagtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag gcatctgagt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 843

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 843

gccttgccag cccgctcagg catctgaaga gtgtctcctc ctacagttcc catagacact 60

gaagttaccc agacaccaaa acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atatggggca cagggctatg tattggtaca agcagaaagc taagaagcca 180

ccggagctca tgtttgtcta cagctatgag aaactctcta taaatgaaag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct ctcttaaacc ttcacctaca cgccctgcag 300

ccagaagact cagccctgta tctctgcgcc agcagtgagc atctgaagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct gcatctgaag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 844

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 844

gccttgccag cccgctcagg catctgagtt ccgaatcctc ctacagttcc catagacact 60

gaagttaccc agacaccaaa acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atatggggca cagggctatg tattggtaca agcagaaagc taagaagcca 180

ccggagctca tgtttgtcta cagctatgag aaactctcta taaatgaaag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct ctcttaaacc ttcacctaca cgccctgcag 300

ccagaagact cagccctgta tctctgcgcc agcagtgagc atctgagttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat gcatctgagt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 845

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 845

gccttgccag cccgctcagg catctgacgt tactttcctc ctacagttcc catagacact 60

gaagttaccc agacaccaaa acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atatggggca cagggctatg tattggtaca agcagaaagc taagaagcca 180

ccggagctca tgtttgtcta cagctatgag aaactctcta taaatgaaag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct ctcttaaacc ttcacctaca cgccctgcag 300

ccagaagact cagccctgta tctctgcgcc agcagtgagc atctgacgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc gcatctgacg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 846

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 846

gccttgccag cccgctcagg catctgatag gagactcctc ctacagttcc catagacact 60

gaagttaccc agacaccaaa acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atatggggca cagggctatg tattggtaca agcagaaagc taagaagcca 180

ccggagctca tgtttgtcta cagctatgag aaactctcta taaatgaaag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct ctcttaaacc ttcacctaca cgccctgcag 300

ccagaagact cagccctgta tctctgcgcc agcagtgagc atctgatagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg gcatctgata ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 847

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 847

gccttgccag cccgctcagg catctgagtg tctactcctc ctacagttcc catagacact 60

gaagttaccc agacaccaaa acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atatggggca cagggctatg tattggtaca agcagaaagc taagaagcca 180

ccggagctca tgtttgtcta cagctatgag aaactctcta taaatgaaag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct ctcttaaacc ttcacctaca cgccctgcag 300

ccagaagact cagccctgta tctctgcgcc agcagtgagc atctgagtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc gcatctgagt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 848

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 848

gccttgccag cccgctcagg catctgagta cagtgtcctc ctacagttcc catagacact 60

gaagttaccc agacaccaaa acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atatggggca cagggctatg tattggtaca agcagaaagc taagaagcca 180

ccggagctca tgtttgtcta cagctatgag aaactctcta taaatgaaag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct ctcttaaacc ttcacctaca cgccctgcag 300

ccagaagact cagccctgta tctctgcgcc agcagtgagc atctgagtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg gcatctgagt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 849

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 849

gccttgccag cccgctcagg catctgagga tcatctcctc ctacagttcc catagacact 60

gaagttaccc agacaccaaa acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atatggggca cagggctatg tattggtaca agcagaaagc taagaagcca 180

ccggagctca tgtttgtcta cagctatgag aaactctcta taaatgaaag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct ctcttaaacc ttcacctaca cgccctgcag 300

ccagaagact cagccctgta tctctgcgcc agcagtgagc atctgaggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg gcatctgagg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 850

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 850

gccttgccag cccgctcagg catctgatat tggcgtcctc ctacagttcc catagacact 60

gaagttaccc agacaccaaa acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atatggggca cagggctatg tattggtaca agcagaaagc taagaagcca 180

ccggagctca tgtttgtcta cagctatgag aaactctcta taaatgaaag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct ctcttaaacc ttcacctaca cgccctgcag 300

ccagaagact cagccctgta tctctgcgcc agcagtgagc atctgatatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg gcatctgata ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 851

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 851

gccttgccag cccgctcagg catctgaagg cttgatcctc ctacagttcc catagacact 60

gaagttaccc agacaccaaa acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atatggggca cagggctatg tattggtaca agcagaaagc taagaagcca 180

ccggagctca tgtttgtcta cagctatgag aaactctcta taaatgaaag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct ctcttaaacc ttcacctaca cgccctgcag 300

ccagaagact cagccctgta tctctgcgcc agcagtgagc atctgaaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg gcatctgaag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 852

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 852

gccttgccag cccgctcagt gctacacgac actcttcccc ccgcagtccc catggaaacg 60

ggagttacgc agacaccaag acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atctggggca taacgctatg tattggtaca agcaaagtgc taagaagcca 180

ctggagctca tgtttgtcta caactttaaa gaacagactg aaaacaacag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct cacttattcc ttcacctaca caccctgcag 300

ccagaagact cggccctgta tctctgtgcc agcagtgatg ctacacgaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag tgctacacga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 853

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 853

gccttgccag cccgctcagt gctacacttc ggaactcccc ccgcagtccc catggaaacg 60

ggagttacgc agacaccaag acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atctggggca taacgctatg tattggtaca agcaaagtgc taagaagcca 180

ctggagctca tgtttgtcta caactttaaa gaacagactg aaaacaacag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct cacttattcc ttcacctaca caccctgcag 300

ccagaagact cggccctgta tctctgtgcc agcagtgatg ctacacttcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat tgctacactt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 854

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 854

gccttgccag cccgctcagt gctacacaag taacgtcccc ccgcagtccc catggaaacg 60

ggagttacgc agacaccaag acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atctggggca taacgctatg tattggtaca agcaaagtgc taagaagcca 180

ctggagctca tgtttgtcta caactttaaa gaacagactg aaaacaacag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct cacttattcc ttcacctaca caccctgcag 300

ccagaagact cggccctgta tctctgtgcc agcagtgatg ctacacaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag tgctacacaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 855

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 855

gccttgccag cccgctcagt gctacacgtc tcctatcccc ccgcagtccc catggaaacg 60

ggagttacgc agacaccaag acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atctggggca taacgctatg tattggtaca agcaaagtgc taagaagcca 180

ctggagctca tgtttgtcta caactttaaa gaacagactg aaaacaacag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct cacttattcc ttcacctaca caccctgcag 300

ccagaagact cggccctgta tctctgtgcc agcagtgatg ctacacgtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag tgctacacgt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 856

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 856

gccttgccag cccgctcagt gctacacaga gtgtctcccc ccgcagtccc catggaaacg 60

ggagttacgc agacaccaag acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atctggggca taacgctatg tattggtaca agcaaagtgc taagaagcca 180

ctggagctca tgtttgtcta caactttaaa gaacagactg aaaacaacag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct cacttattcc ttcacctaca caccctgcag 300

ccagaagact cggccctgta tctctgtgcc agcagtgatg ctacacagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct tgctacacag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 857

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 857

gccttgccag cccgctcagt gctacacgtt ccgaatcccc ccgcagtccc catggaaacg 60

ggagttacgc agacaccaag acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atctggggca taacgctatg tattggtaca agcaaagtgc taagaagcca 180

ctggagctca tgtttgtcta caactttaaa gaacagactg aaaacaacag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct cacttattcc ttcacctaca caccctgcag 300

ccagaagact cggccctgta tctctgtgcc agcagtgatg ctacacgttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat tgctacacgt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 858

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 858

gccttgccag cccgctcagt gctacaccgt tactttcccc ccgcagtccc catggaaacg 60

ggagttacgc agacaccaag acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atctggggca taacgctatg tattggtaca agcaaagtgc taagaagcca 180

ctggagctca tgtttgtcta caactttaaa gaacagactg aaaacaacag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct cacttattcc ttcacctaca caccctgcag 300

ccagaagact cggccctgta tctctgtgcc agcagtgatg ctacaccgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc tgctacaccg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 859

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 859

gccttgccag cccgctcagt gctacactag gagactcccc ccgcagtccc catggaaacg 60

ggagttacgc agacaccaag acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atctggggca taacgctatg tattggtaca agcaaagtgc taagaagcca 180

ctggagctca tgtttgtcta caactttaaa gaacagactg aaaacaacag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct cacttattcc ttcacctaca caccctgcag 300

ccagaagact cggccctgta tctctgtgcc agcagtgatg ctacactagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg tgctacacta ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 860

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 860

gccttgccag cccgctcagt gctacacgtg tctactcccc ccgcagtccc catggaaacg 60

ggagttacgc agacaccaag acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atctggggca taacgctatg tattggtaca agcaaagtgc taagaagcca 180

ctggagctca tgtttgtcta caactttaaa gaacagactg aaaacaacag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct cacttattcc ttcacctaca caccctgcag 300

ccagaagact cggccctgta tctctgtgcc agcagtgatg ctacacgtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc tgctacacgt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 861

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 861

gccttgccag cccgctcagt gctacacgta cagtgtcccc ccgcagtccc catggaaacg 60

ggagttacgc agacaccaag acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atctggggca taacgctatg tattggtaca agcaaagtgc taagaagcca 180

ctggagctca tgtttgtcta caactttaaa gaacagactg aaaacaacag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct cacttattcc ttcacctaca caccctgcag 300

ccagaagact cggccctgta tctctgtgcc agcagtgatg ctacacgtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg tgctacacgt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 862

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 862

gccttgccag cccgctcagt gctacacgga tcatctcccc ccgcagtccc catggaaacg 60

ggagttacgc agacaccaag acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atctggggca taacgctatg tattggtaca agcaaagtgc taagaagcca 180

ctggagctca tgtttgtcta caactttaaa gaacagactg aaaacaacag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct cacttattcc ttcacctaca caccctgcag 300

ccagaagact cggccctgta tctctgtgcc agcagtgatg ctacacggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg tgctacacgg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 863

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 863

gccttgccag cccgctcagt gctacactat tggcgtcccc ccgcagtccc catggaaacg 60

ggagttacgc agacaccaag acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atctggggca taacgctatg tattggtaca agcaaagtgc taagaagcca 180

ctggagctca tgtttgtcta caactttaaa gaacagactg aaaacaacag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct cacttattcc ttcacctaca caccctgcag 300

ccagaagact cggccctgta tctctgtgcc agcagtgatg ctacactatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg tgctacacta ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 864

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 864

gccttgccag cccgctcagt gctacacagg cttgatcccc ccgcagtccc catggaaacg 60

ggagttacgc agacaccaag acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atctggggca taacgctatg tattggtaca agcaaagtgc taagaagcca 180

ctggagctca tgtttgtcta caactttaaa gaacagactg aaaacaacag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct cacttattcc ttcacctaca caccctgcag 300

ccagaagact cggccctgta tctctgtgcc agcagtgatg ctacacaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg tgctacacag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 865

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 865

gccttgccag cccgctcaga actgccagac actcttcccc ccacagtccc catggaaacg 60

ggagttacgc agacaccaag acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atctgggtca taacgctatg tattggtaca agcaaagtgc taagaagcca 180

ctggagctca tgtttgtcta cagtcttgaa gaacgggttg aaaacaacag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct cacttattcc ttcacctaca caccctgcag 300

ccagaagact cggccctgta tctctgcgcc agcagtgaaa ctgccagaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag aactgccaga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 866

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 866

gccttgccag cccgctcaga actgccattc ggaactcccc ccacagtccc catggaaacg 60

ggagttacgc agacaccaag acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atctgggtca taacgctatg tattggtaca agcaaagtgc taagaagcca 180

ctggagctca tgtttgtcta cagtcttgaa gaacgggttg aaaacaacag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct cacttattcc ttcacctaca caccctgcag 300

ccagaagact cggccctgta tctctgcgcc agcagtgaaa ctgccattcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat aactgccatt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 867

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 867

gccttgccag cccgctcaga actgccaaag taacgtcccc ccacagtccc catggaaacg 60

ggagttacgc agacaccaag acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atctgggtca taacgctatg tattggtaca agcaaagtgc taagaagcca 180

ctggagctca tgtttgtcta cagtcttgaa gaacgggttg aaaacaacag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct cacttattcc ttcacctaca caccctgcag 300

ccagaagact cggccctgta tctctgcgcc agcagtgaaa ctgccaaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag aactgccaaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 868

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 868

gccttgccag cccgctcaga actgccagtc tcctatcccc ccacagtccc catggaaacg 60

ggagttacgc agacaccaag acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atctgggtca taacgctatg tattggtaca agcaaagtgc taagaagcca 180

ctggagctca tgtttgtcta cagtcttgaa gaacgggttg aaaacaacag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct cacttattcc ttcacctaca caccctgcag 300

ccagaagact cggccctgta tctctgcgcc agcagtgaaa ctgccagtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag aactgccagt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 869

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 869

gccttgccag cccgctcaga actgccaaga gtgtctcccc ccacagtccc catggaaacg 60

ggagttacgc agacaccaag acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atctgggtca taacgctatg tattggtaca agcaaagtgc taagaagcca 180

ctggagctca tgtttgtcta cagtcttgaa gaacgggttg aaaacaacag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct cacttattcc ttcacctaca caccctgcag 300

ccagaagact cggccctgta tctctgcgcc agcagtgaaa ctgccaagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct aactgccaag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 870

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 870

gccttgccag cccgctcaga actgccagtt ccgaatcccc ccacagtccc catggaaacg 60

ggagttacgc agacaccaag acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atctgggtca taacgctatg tattggtaca agcaaagtgc taagaagcca 180

ctggagctca tgtttgtcta cagtcttgaa gaacgggttg aaaacaacag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct cacttattcc ttcacctaca caccctgcag 300

ccagaagact cggccctgta tctctgcgcc agcagtgaaa ctgccagttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat aactgccagt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 871

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 871

gccttgccag cccgctcaga actgccacgt tactttcccc ccacagtccc catggaaacg 60

ggagttacgc agacaccaag acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atctgggtca taacgctatg tattggtaca agcaaagtgc taagaagcca 180

ctggagctca tgtttgtcta cagtcttgaa gaacgggttg aaaacaacag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct cacttattcc ttcacctaca caccctgcag 300

ccagaagact cggccctgta tctctgcgcc agcagtgaaa ctgccacgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc aactgccacg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 872

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 872

gccttgccag cccgctcaga actgccatag gagactcccc ccacagtccc catggaaacg 60

ggagttacgc agacaccaag acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atctgggtca taacgctatg tattggtaca agcaaagtgc taagaagcca 180

ctggagctca tgtttgtcta cagtcttgaa gaacgggttg aaaacaacag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct cacttattcc ttcacctaca caccctgcag 300

ccagaagact cggccctgta tctctgcgcc agcagtgaaa ctgccatagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg aactgccata ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 873

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 873

gccttgccag cccgctcaga actgccagtg tctactcccc ccacagtccc catggaaacg 60

ggagttacgc agacaccaag acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atctgggtca taacgctatg tattggtaca agcaaagtgc taagaagcca 180

ctggagctca tgtttgtcta cagtcttgaa gaacgggttg aaaacaacag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct cacttattcc ttcacctaca caccctgcag 300

ccagaagact cggccctgta tctctgcgcc agcagtgaaa ctgccagtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc aactgccagt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 874

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 874

gccttgccag cccgctcaga actgccagta cagtgtcccc ccacagtccc catggaaacg 60

ggagttacgc agacaccaag acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atctgggtca taacgctatg tattggtaca agcaaagtgc taagaagcca 180

ctggagctca tgtttgtcta cagtcttgaa gaacgggttg aaaacaacag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct cacttattcc ttcacctaca caccctgcag 300

ccagaagact cggccctgta tctctgcgcc agcagtgaaa ctgccagtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg aactgccagt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 875

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 875

gccttgccag cccgctcaga actgccagga tcatctcccc ccacagtccc catggaaacg 60

ggagttacgc agacaccaag acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atctgggtca taacgctatg tattggtaca agcaaagtgc taagaagcca 180

ctggagctca tgtttgtcta cagtcttgaa gaacgggttg aaaacaacag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct cacttattcc ttcacctaca caccctgcag 300

ccagaagact cggccctgta tctctgcgcc agcagtgaaa ctgccaggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg aactgccagg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 876

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 876

gccttgccag cccgctcaga actgccatat tggcgtcccc ccacagtccc catggaaacg 60

ggagttacgc agacaccaag acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atctgggtca taacgctatg tattggtaca agcaaagtgc taagaagcca 180

ctggagctca tgtttgtcta cagtcttgaa gaacgggttg aaaacaacag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct cacttattcc ttcacctaca caccctgcag 300

ccagaagact cggccctgta tctctgcgcc agcagtgaaa ctgccatatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg aactgccata ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 877

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 877

gccttgccag cccgctcaga actgccaagg cttgatcccc ccacagtccc catggaaacg 60

ggagttacgc agacaccaag acacctggtc atgggaatga caaataagaa gtctttgaaa 120

tgtgaacaac atctgggtca taacgctatg tattggtaca agcaaagtgc taagaagcca 180

ctggagctca tgtttgtcta cagtcttgaa gaacgggttg aaaacaacag tgtgccaagt 240

cgcttctcac ctgaatgccc caacagctct cacttattcc ttcacctaca caccctgcag 300

ccagaagact cggccctgta tctctgcgcc agcagtgaaa ctgccaaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg aactgccaag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 878

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 878

gccttgccag cccgctcagt tggactggac actctctttt cccacaggcc cagtaaaggc 60

tggagtcact caaactccaa gatatctgat caaaacgaga ggacagcaag tgacactgag 120

ctgctcccct atctctgggc ataggagtgt atcctggtac caacagaccc caggacaggg 180

ccttcagttc ctctttgaat acttcagtga gacacagaga aacaaaggaa acttccctgg 240

tcgattctca gggcgccagt tctctaactc tcgctctgag atgaatgtga gcaccttgga 300

gctgggggac tcggcccttt atctttgcgc cagcatgatt ggactggaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag ttggactgga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 879

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 879

gccttgccag cccgctcagt tggactgttc ggaacctttt cccacaggcc cagtaaaggc 60

tggagtcact caaactccaa gatatctgat caaaacgaga ggacagcaag tgacactgag 120

ctgctcccct atctctgggc ataggagtgt atcctggtac caacagaccc caggacaggg 180

ccttcagttc ctctttgaat acttcagtga gacacagaga aacaaaggaa acttccctgg 240

tcgattctca gggcgccagt tctctaactc tcgctctgag atgaatgtga gcaccttgga 300

gctgggggac tcggcccttt atctttgcgc cagcatgatt ggactgttcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat ttggactgtt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 880

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 880

gccttgccag cccgctcagt tggactgaag taacgctttt cccacaggcc cagtaaaggc 60

tggagtcact caaactccaa gatatctgat caaaacgaga ggacagcaag tgacactgag 120

ctgctcccct atctctgggc ataggagtgt atcctggtac caacagaccc caggacaggg 180

ccttcagttc ctctttgaat acttcagtga gacacagaga aacaaaggaa acttccctgg 240

tcgattctca gggcgccagt tctctaactc tcgctctgag atgaatgtga gcaccttgga 300

gctgggggac tcggcccttt atctttgcgc cagcatgatt ggactgaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag ttggactgaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 881

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 881

gccttgccag cccgctcagt tggactggtc tcctactttt cccacaggcc cagtaaaggc 60

tggagtcact caaactccaa gatatctgat caaaacgaga ggacagcaag tgacactgag 120

ctgctcccct atctctgggc ataggagtgt atcctggtac caacagaccc caggacaggg 180

ccttcagttc ctctttgaat acttcagtga gacacagaga aacaaaggaa acttccctgg 240

tcgattctca gggcgccagt tctctaactc tcgctctgag atgaatgtga gcaccttgga 300

gctgggggac tcggcccttt atctttgcgc cagcatgatt ggactggtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag ttggactggt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 882

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 882

gccttgccag cccgctcagt tggactgaga gtgtcctttt cccacaggcc cagtaaaggc 60

tggagtcact caaactccaa gatatctgat caaaacgaga ggacagcaag tgacactgag 120

ctgctcccct atctctgggc ataggagtgt atcctggtac caacagaccc caggacaggg 180

ccttcagttc ctctttgaat acttcagtga gacacagaga aacaaaggaa acttccctgg 240

tcgattctca gggcgccagt tctctaactc tcgctctgag atgaatgtga gcaccttgga 300

gctgggggac tcggcccttt atctttgcgc cagcatgatt ggactgagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct ttggactgag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 883

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 883

gccttgccag cccgctcagt tggactggtt ccgaactttt cccacaggcc cagtaaaggc 60

tggagtcact caaactccaa gatatctgat caaaacgaga ggacagcaag tgacactgag 120

ctgctcccct atctctgggc ataggagtgt atcctggtac caacagaccc caggacaggg 180

ccttcagttc ctctttgaat acttcagtga gacacagaga aacaaaggaa acttccctgg 240

tcgattctca gggcgccagt tctctaactc tcgctctgag atgaatgtga gcaccttgga 300

gctgggggac tcggcccttt atctttgcgc cagcatgatt ggactggttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat ttggactggt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 884

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 884

gccttgccag cccgctcagt tggactgcgt tacttctttt cccacaggcc cagtaaaggc 60

tggagtcact caaactccaa gatatctgat caaaacgaga ggacagcaag tgacactgag 120

ctgctcccct atctctgggc ataggagtgt atcctggtac caacagaccc caggacaggg 180

ccttcagttc ctctttgaat acttcagtga gacacagaga aacaaaggaa acttccctgg 240

tcgattctca gggcgccagt tctctaactc tcgctctgag atgaatgtga gcaccttgga 300

gctgggggac tcggcccttt atctttgcgc cagcatgatt ggactgcgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc ttggactgcg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 885

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 885

gccttgccag cccgctcagt tggactgtag gagacctttt cccacaggcc cagtaaaggc 60

tggagtcact caaactccaa gatatctgat caaaacgaga ggacagcaag tgacactgag 120

ctgctcccct atctctgggc ataggagtgt atcctggtac caacagaccc caggacaggg 180

ccttcagttc ctctttgaat acttcagtga gacacagaga aacaaaggaa acttccctgg 240

tcgattctca gggcgccagt tctctaactc tcgctctgag atgaatgtga gcaccttgga 300

gctgggggac tcggcccttt atctttgcgc cagcatgatt ggactgtagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg ttggactgta ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 886

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 886

gccttgccag cccgctcagt tggactggtg tctacctttt cccacaggcc cagtaaaggc 60

tggagtcact caaactccaa gatatctgat caaaacgaga ggacagcaag tgacactgag 120

ctgctcccct atctctgggc ataggagtgt atcctggtac caacagaccc caggacaggg 180

ccttcagttc ctctttgaat acttcagtga gacacagaga aacaaaggaa acttccctgg 240

tcgattctca gggcgccagt tctctaactc tcgctctgag atgaatgtga gcaccttgga 300

gctgggggac tcggcccttt atctttgcgc cagcatgatt ggactggtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc ttggactggt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 887

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 887

gccttgccag cccgctcagt tggactggta cagtgctttt cccacaggcc cagtaaaggc 60

tggagtcact caaactccaa gatatctgat caaaacgaga ggacagcaag tgacactgag 120

ctgctcccct atctctgggc ataggagtgt atcctggtac caacagaccc caggacaggg 180

ccttcagttc ctctttgaat acttcagtga gacacagaga aacaaaggaa acttccctgg 240

tcgattctca gggcgccagt tctctaactc tcgctctgag atgaatgtga gcaccttgga 300

gctgggggac tcggcccttt atctttgcgc cagcatgatt ggactggtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg ttggactggt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 888

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 888

gccttgccag cccgctcagt tggactggga tcatcctttt cccacaggcc cagtaaaggc 60

tggagtcact caaactccaa gatatctgat caaaacgaga ggacagcaag tgacactgag 120

ctgctcccct atctctgggc ataggagtgt atcctggtac caacagaccc caggacaggg 180

ccttcagttc ctctttgaat acttcagtga gacacagaga aacaaaggaa acttccctgg 240

tcgattctca gggcgccagt tctctaactc tcgctctgag atgaatgtga gcaccttgga 300

gctgggggac tcggcccttt atctttgcgc cagcatgatt ggactgggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg ttggactggg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 889

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 889

gccttgccag cccgctcagt tggactgtat tggcgctttt cccacaggcc cagtaaaggc 60

tggagtcact caaactccaa gatatctgat caaaacgaga ggacagcaag tgacactgag 120

ctgctcccct atctctgggc ataggagtgt atcctggtac caacagaccc caggacaggg 180

ccttcagttc ctctttgaat acttcagtga gacacagaga aacaaaggaa acttccctgg 240

tcgattctca gggcgccagt tctctaactc tcgctctgag atgaatgtga gcaccttgga 300

gctgggggac tcggcccttt atctttgcgc cagcatgatt ggactgtatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg ttggactgta ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 890

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 890

gccttgccag cccgctcagt tggactgagg cttgactttt cccacaggcc cagtaaaggc 60

tggagtcact caaactccaa gatatctgat caaaacgaga ggacagcaag tgacactgag 120

ctgctcccct atctctgggc ataggagtgt atcctggtac caacagaccc caggacaggg 180

ccttcagttc ctctttgaat acttcagtga gacacagaga aacaaaggaa acttccctgg 240

tcgattctca gggcgccagt tctctaactc tcgctctgag atgaatgtga gcaccttgga 300

gctgggggac tcggcccttt atctttgcgc cagcatgatt ggactgaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg ttggactgag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 891

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 891

gccttgccag cccgctcagg tagacacgac actctttctc cacctatttt ttcctcaggc 60

ccagtggagg ctggaatcac ccaagctcca agacacctga tcaaaacaag agaccagcaa 120

gtgacactga gatgctcccc tgcctctggg cataactgtg tgtcctggta cctacgaact 180

ccaagtcagc ccctctagtt attgttacaa tattgtaata ggttacaaag agcaaaagga 240

aacttgccta attgattctc agctcaccac gtccataact attactgagt caaacacgga 300

gctaggggac tcagccctgt atctctgtgc cagcatgagt agacacgaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag gtagacacga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 892

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 892

gccttgccag cccgctcagg tagacacttc ggaacttctc cacctatttt ttcctcaggc 60

ccagtggagg ctggaatcac ccaagctcca agacacctga tcaaaacaag agaccagcaa 120

gtgacactga gatgctcccc tgcctctggg cataactgtg tgtcctggta cctacgaact 180

ccaagtcagc ccctctagtt attgttacaa tattgtaata ggttacaaag agcaaaagga 240

aacttgccta attgattctc agctcaccac gtccataact attactgagt caaacacgga 300

gctaggggac tcagccctgt atctctgtgc cagcatgagt agacacttcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat gtagacactt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 893

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 893

gccttgccag cccgctcagg tagacacaag taacgttctc cacctatttt ttcctcaggc 60

ccagtggagg ctggaatcac ccaagctcca agacacctga tcaaaacaag agaccagcaa 120

gtgacactga gatgctcccc tgcctctggg cataactgtg tgtcctggta cctacgaact 180

ccaagtcagc ccctctagtt attgttacaa tattgtaata ggttacaaag agcaaaagga 240

aacttgccta attgattctc agctcaccac gtccataact attactgagt caaacacgga 300

gctaggggac tcagccctgt atctctgtgc cagcatgagt agacacaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag gtagacacaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 894

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 894

gccttgccag cccgctcagg tagacacgtc tcctattctc cacctatttt ttcctcaggc 60

ccagtggagg ctggaatcac ccaagctcca agacacctga tcaaaacaag agaccagcaa 120

gtgacactga gatgctcccc tgcctctggg cataactgtg tgtcctggta cctacgaact 180

ccaagtcagc ccctctagtt attgttacaa tattgtaata ggttacaaag agcaaaagga 240

aacttgccta attgattctc agctcaccac gtccataact attactgagt caaacacgga 300

gctaggggac tcagccctgt atctctgtgc cagcatgagt agacacgtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag gtagacacgt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 895

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 895

gccttgccag cccgctcagg tagacacaga gtgtcttctc cacctatttt ttcctcaggc 60

ccagtggagg ctggaatcac ccaagctcca agacacctga tcaaaacaag agaccagcaa 120

gtgacactga gatgctcccc tgcctctggg cataactgtg tgtcctggta cctacgaact 180

ccaagtcagc ccctctagtt attgttacaa tattgtaata ggttacaaag agcaaaagga 240

aacttgccta attgattctc agctcaccac gtccataact attactgagt caaacacgga 300

gctaggggac tcagccctgt atctctgtgc cagcatgagt agacacagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct gtagacacag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 896

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 896

gccttgccag cccgctcagg tagacacgtt ccgaattctc cacctatttt ttcctcaggc 60

ccagtggagg ctggaatcac ccaagctcca agacacctga tcaaaacaag agaccagcaa 120

gtgacactga gatgctcccc tgcctctggg cataactgtg tgtcctggta cctacgaact 180

ccaagtcagc ccctctagtt attgttacaa tattgtaata ggttacaaag agcaaaagga 240

aacttgccta attgattctc agctcaccac gtccataact attactgagt caaacacgga 300

gctaggggac tcagccctgt atctctgtgc cagcatgagt agacacgttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat gtagacacgt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 897

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 897

gccttgccag cccgctcagg tagacaccgt tacttttctc cacctatttt ttcctcaggc 60

ccagtggagg ctggaatcac ccaagctcca agacacctga tcaaaacaag agaccagcaa 120

gtgacactga gatgctcccc tgcctctggg cataactgtg tgtcctggta cctacgaact 180

ccaagtcagc ccctctagtt attgttacaa tattgtaata ggttacaaag agcaaaagga 240

aacttgccta attgattctc agctcaccac gtccataact attactgagt caaacacgga 300

gctaggggac tcagccctgt atctctgtgc cagcatgagt agacaccgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc gtagacaccg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 898

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 898

gccttgccag cccgctcagg tagacactag gagacttctc cacctatttt ttcctcaggc 60

ccagtggagg ctggaatcac ccaagctcca agacacctga tcaaaacaag agaccagcaa 120

gtgacactga gatgctcccc tgcctctggg cataactgtg tgtcctggta cctacgaact 180

ccaagtcagc ccctctagtt attgttacaa tattgtaata ggttacaaag agcaaaagga 240

aacttgccta attgattctc agctcaccac gtccataact attactgagt caaacacgga 300

gctaggggac tcagccctgt atctctgtgc cagcatgagt agacactagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg gtagacacta ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 899

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 899

gccttgccag cccgctcagg tagacacgtg tctacttctc cacctatttt ttcctcaggc 60

ccagtggagg ctggaatcac ccaagctcca agacacctga tcaaaacaag agaccagcaa 120

gtgacactga gatgctcccc tgcctctggg cataactgtg tgtcctggta cctacgaact 180

ccaagtcagc ccctctagtt attgttacaa tattgtaata ggttacaaag agcaaaagga 240

aacttgccta attgattctc agctcaccac gtccataact attactgagt caaacacgga 300

gctaggggac tcagccctgt atctctgtgc cagcatgagt agacacgtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc gtagacacgt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 900

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 900

gccttgccag cccgctcagg tagacacgta cagtgttctc cacctatttt ttcctcaggc 60

ccagtggagg ctggaatcac ccaagctcca agacacctga tcaaaacaag agaccagcaa 120

gtgacactga gatgctcccc tgcctctggg cataactgtg tgtcctggta cctacgaact 180

ccaagtcagc ccctctagtt attgttacaa tattgtaata ggttacaaag agcaaaagga 240

aacttgccta attgattctc agctcaccac gtccataact attactgagt caaacacgga 300

gctaggggac tcagccctgt atctctgtgc cagcatgagt agacacgtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg gtagacacgt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 901

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 901

gccttgccag cccgctcagg tagacacgga tcatcttctc cacctatttt ttcctcaggc 60

ccagtggagg ctggaatcac ccaagctcca agacacctga tcaaaacaag agaccagcaa 120

gtgacactga gatgctcccc tgcctctggg cataactgtg tgtcctggta cctacgaact 180

ccaagtcagc ccctctagtt attgttacaa tattgtaata ggttacaaag agcaaaagga 240

aacttgccta attgattctc agctcaccac gtccataact attactgagt caaacacgga 300

gctaggggac tcagccctgt atctctgtgc cagcatgagt agacacggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg gtagacacgg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 902

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 902

gccttgccag cccgctcagg tagacactat tggcgttctc cacctatttt ttcctcaggc 60

ccagtggagg ctggaatcac ccaagctcca agacacctga tcaaaacaag agaccagcaa 120

gtgacactga gatgctcccc tgcctctggg cataactgtg tgtcctggta cctacgaact 180

ccaagtcagc ccctctagtt attgttacaa tattgtaata ggttacaaag agcaaaagga 240

aacttgccta attgattctc agctcaccac gtccataact attactgagt caaacacgga 300

gctaggggac tcagccctgt atctctgtgc cagcatgagt agacactatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg gtagacacta ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 903

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 903

gccttgccag cccgctcagg tagacacagg cttgattctc cacctatttt ttcctcaggc 60

ccagtggagg ctggaatcac ccaagctcca agacacctga tcaaaacaag agaccagcaa 120

gtgacactga gatgctcccc tgcctctggg cataactgtg tgtcctggta cctacgaact 180

ccaagtcagc ccctctagtt attgttacaa tattgtaata ggttacaaag agcaaaagga 240

aacttgccta attgattctc agctcaccac gtccataact attactgagt caaacacgga 300

gctaggggac tcagccctgt atctctgtgc cagcatgagt agacacaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg gtagacacag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 904

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 904

gccttgccag cccgctcagc actgtacgac actctctttt cccacaggcc cagtggaggc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct atctctgggc acagcagtgt gtcctggtac caacaggccc cgggtcaggg 180

gccccagttt atctttgaat atgctaatga gttaaggaga tcagaaggaa acttccctaa 240

tcgattctca gggcgccagt tccatgactg ttgctctgag atgaatgtga gtgccttgga 300

gctgggggac tcggccctgt atctctgtgc cagaatgaca ctgtacgaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag cactgtacga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 905

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 905

gccttgccag cccgctcagc actgtacttc ggaacctttt cccacaggcc cagtggaggc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct atctctgggc acagcagtgt gtcctggtac caacaggccc cgggtcaggg 180

gccccagttt atctttgaat atgctaatga gttaaggaga tcagaaggaa acttccctaa 240

tcgattctca gggcgccagt tccatgactg ttgctctgag atgaatgtga gtgccttgga 300

gctgggggac tcggccctgt atctctgtgc cagaatgaca ctgtacttcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat cactgtactt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 906

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 906

gccttgccag cccgctcagc actgtacaag taacgctttt cccacaggcc cagtggaggc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct atctctgggc acagcagtgt gtcctggtac caacaggccc cgggtcaggg 180

gccccagttt atctttgaat atgctaatga gttaaggaga tcagaaggaa acttccctaa 240

tcgattctca gggcgccagt tccatgactg ttgctctgag atgaatgtga gtgccttgga 300

gctgggggac tcggccctgt atctctgtgc cagaatgaca ctgtacaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag cactgtacaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 907

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 907

gccttgccag cccgctcagc actgtacgtc tcctactttt cccacaggcc cagtggaggc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct atctctgggc acagcagtgt gtcctggtac caacaggccc cgggtcaggg 180

gccccagttt atctttgaat atgctaatga gttaaggaga tcagaaggaa acttccctaa 240

tcgattctca gggcgccagt tccatgactg ttgctctgag atgaatgtga gtgccttgga 300

gctgggggac tcggccctgt atctctgtgc cagaatgaca ctgtacgtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag cactgtacgt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 908

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 908

gccttgccag cccgctcagc actgtacaga gtgtcctttt cccacaggcc cagtggaggc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct atctctgggc acagcagtgt gtcctggtac caacaggccc cgggtcaggg 180

gccccagttt atctttgaat atgctaatga gttaaggaga tcagaaggaa acttccctaa 240

tcgattctca gggcgccagt tccatgactg ttgctctgag atgaatgtga gtgccttgga 300

gctgggggac tcggccctgt atctctgtgc cagaatgaca ctgtacagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct cactgtacag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 909

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 909

gccttgccag cccgctcagc actgtacgtt ccgaactttt cccacaggcc cagtggaggc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct atctctgggc acagcagtgt gtcctggtac caacaggccc cgggtcaggg 180

gccccagttt atctttgaat atgctaatga gttaaggaga tcagaaggaa acttccctaa 240

tcgattctca gggcgccagt tccatgactg ttgctctgag atgaatgtga gtgccttgga 300

gctgggggac tcggccctgt atctctgtgc cagaatgaca ctgtacgttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat cactgtacgt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 910

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 910

gccttgccag cccgctcagc actgtaccgt tacttctttt cccacaggcc cagtggaggc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct atctctgggc acagcagtgt gtcctggtac caacaggccc cgggtcaggg 180

gccccagttt atctttgaat atgctaatga gttaaggaga tcagaaggaa acttccctaa 240

tcgattctca gggcgccagt tccatgactg ttgctctgag atgaatgtga gtgccttgga 300

gctgggggac tcggccctgt atctctgtgc cagaatgaca ctgtaccgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc cactgtaccg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 911

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 911

gccttgccag cccgctcagc actgtactag gagacctttt cccacaggcc cagtggaggc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct atctctgggc acagcagtgt gtcctggtac caacaggccc cgggtcaggg 180

gccccagttt atctttgaat atgctaatga gttaaggaga tcagaaggaa acttccctaa 240

tcgattctca gggcgccagt tccatgactg ttgctctgag atgaatgtga gtgccttgga 300

gctgggggac tcggccctgt atctctgtgc cagaatgaca ctgtactagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg cactgtacta ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 912

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 912

gccttgccag cccgctcagc actgtacgtg tctacctttt cccacaggcc cagtggaggc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct atctctgggc acagcagtgt gtcctggtac caacaggccc cgggtcaggg 180

gccccagttt atctttgaat atgctaatga gttaaggaga tcagaaggaa acttccctaa 240

tcgattctca gggcgccagt tccatgactg ttgctctgag atgaatgtga gtgccttgga 300

gctgggggac tcggccctgt atctctgtgc cagaatgaca ctgtacgtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc cactgtacgt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 913

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 913

gccttgccag cccgctcagc actgtacgta cagtgctttt cccacaggcc cagtggaggc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct atctctgggc acagcagtgt gtcctggtac caacaggccc cgggtcaggg 180

gccccagttt atctttgaat atgctaatga gttaaggaga tcagaaggaa acttccctaa 240

tcgattctca gggcgccagt tccatgactg ttgctctgag atgaatgtga gtgccttgga 300

gctgggggac tcggccctgt atctctgtgc cagaatgaca ctgtacgtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg cactgtacgt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 914

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 914

gccttgccag cccgctcagc actgtacgga tcatcctttt cccacaggcc cagtggaggc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct atctctgggc acagcagtgt gtcctggtac caacaggccc cgggtcaggg 180

gccccagttt atctttgaat atgctaatga gttaaggaga tcagaaggaa acttccctaa 240

tcgattctca gggcgccagt tccatgactg ttgctctgag atgaatgtga gtgccttgga 300

gctgggggac tcggccctgt atctctgtgc cagaatgaca ctgtacggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg cactgtacgg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 915

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 915

gccttgccag cccgctcagc actgtactat tggcgctttt cccacaggcc cagtggaggc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct atctctgggc acagcagtgt gtcctggtac caacaggccc cgggtcaggg 180

gccccagttt atctttgaat atgctaatga gttaaggaga tcagaaggaa acttccctaa 240

tcgattctca gggcgccagt tccatgactg ttgctctgag atgaatgtga gtgccttgga 300

gctgggggac tcggccctgt atctctgtgc cagaatgaca ctgtactatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg cactgtacta ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 916

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 916

gccttgccag cccgctcagc actgtacagg cttgactttt cccacaggcc cagtggaggc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct atctctgggc acagcagtgt gtcctggtac caacaggccc cgggtcaggg 180

gccccagttt atctttgaat atgctaatga gttaaggaga tcagaaggaa acttccctaa 240

tcgattctca gggcgccagt tccatgactg ttgctctgag atgaatgtga gtgccttgga 300

gctgggggac tcggccctgt atctctgtgc cagaatgaca ctgtacaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg cactgtacag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 917

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 917

gccttgccag cccgctcagg atgatccgac actctctttt cccacaggct cagtggagac 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctcttct cagtctgggc acaacactgt gtcctggtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attataggga ggaagagaat ggcagaggaa acttccctcc 240

tagattctca ggtctccagt tccctaatta tagctctgag ctgaatgtga acgccttgga 300

gctggacgac tcggccctgt atctctgtgc cagcatgaga tgatccgaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag gatgatccga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 918

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 918

gccttgccag cccgctcagg atgatccttc ggaacctttt cccacaggct cagtggagac 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctcttct cagtctgggc acaacactgt gtcctggtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attataggga ggaagagaat ggcagaggaa acttccctcc 240

tagattctca ggtctccagt tccctaatta tagctctgag ctgaatgtga acgccttgga 300

gctggacgac tcggccctgt atctctgtgc cagcatgaga tgatccttcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat gatgatcctt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 919

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 919

gccttgccag cccgctcagg atgatccaag taacgctttt cccacaggct cagtggagac 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctcttct cagtctgggc acaacactgt gtcctggtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attataggga ggaagagaat ggcagaggaa acttccctcc 240

tagattctca ggtctccagt tccctaatta tagctctgag ctgaatgtga acgccttgga 300

gctggacgac tcggccctgt atctctgtgc cagcatgaga tgatccaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag gatgatccaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 920

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 920

gccttgccag cccgctcagg atgatccgtc tcctactttt cccacaggct cagtggagac 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctcttct cagtctgggc acaacactgt gtcctggtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attataggga ggaagagaat ggcagaggaa acttccctcc 240

tagattctca ggtctccagt tccctaatta tagctctgag ctgaatgtga acgccttgga 300

gctggacgac tcggccctgt atctctgtgc cagcatgaga tgatccgtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag gatgatccgt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 921

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 921

gccttgccag cccgctcagg atgatccaga gtgtcctttt cccacaggct cagtggagac 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctcttct cagtctgggc acaacactgt gtcctggtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attataggga ggaagagaat ggcagaggaa acttccctcc 240

tagattctca ggtctccagt tccctaatta tagctctgag ctgaatgtga acgccttgga 300

gctggacgac tcggccctgt atctctgtgc cagcatgaga tgatccagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct gatgatccag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 922

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 922

gccttgccag cccgctcagg atgatccgtt ccgaactttt cccacaggct cagtggagac 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctcttct cagtctgggc acaacactgt gtcctggtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attataggga ggaagagaat ggcagaggaa acttccctcc 240

tagattctca ggtctccagt tccctaatta tagctctgag ctgaatgtga acgccttgga 300

gctggacgac tcggccctgt atctctgtgc cagcatgaga tgatccgttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat gatgatccgt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 923

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 923

gccttgccag cccgctcagg atgatcccgt tacttctttt cccacaggct cagtggagac 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctcttct cagtctgggc acaacactgt gtcctggtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attataggga ggaagagaat ggcagaggaa acttccctcc 240

tagattctca ggtctccagt tccctaatta tagctctgag ctgaatgtga acgccttgga 300

gctggacgac tcggccctgt atctctgtgc cagcatgaga tgatcccgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc gatgatcccg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 924

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 924

gccttgccag cccgctcagg atgatcctag gagacctttt cccacaggct cagtggagac 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctcttct cagtctgggc acaacactgt gtcctggtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attataggga ggaagagaat ggcagaggaa acttccctcc 240

tagattctca ggtctccagt tccctaatta tagctctgag ctgaatgtga acgccttgga 300

gctggacgac tcggccctgt atctctgtgc cagcatgaga tgatcctagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg gatgatccta ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 925

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 925

gccttgccag cccgctcagg atgatccgtg tctacctttt cccacaggct cagtggagac 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctcttct cagtctgggc acaacactgt gtcctggtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attataggga ggaagagaat ggcagaggaa acttccctcc 240

tagattctca ggtctccagt tccctaatta tagctctgag ctgaatgtga acgccttgga 300

gctggacgac tcggccctgt atctctgtgc cagcatgaga tgatccgtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc gatgatccgt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 926

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 926

gccttgccag cccgctcagg atgatccgta cagtgctttt cccacaggct cagtggagac 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctcttct cagtctgggc acaacactgt gtcctggtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attataggga ggaagagaat ggcagaggaa acttccctcc 240

tagattctca ggtctccagt tccctaatta tagctctgag ctgaatgtga acgccttgga 300

gctggacgac tcggccctgt atctctgtgc cagcatgaga tgatccgtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg gatgatccgt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 927

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 927

gccttgccag cccgctcagg atgatccgga tcatcctttt cccacaggct cagtggagac 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctcttct cagtctgggc acaacactgt gtcctggtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attataggga ggaagagaat ggcagaggaa acttccctcc 240

tagattctca ggtctccagt tccctaatta tagctctgag ctgaatgtga acgccttgga 300

gctggacgac tcggccctgt atctctgtgc cagcatgaga tgatccggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg gatgatccgg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 928

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 928

gccttgccag cccgctcagg atgatcctat tggcgctttt cccacaggct cagtggagac 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctcttct cagtctgggc acaacactgt gtcctggtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attataggga ggaagagaat ggcagaggaa acttccctcc 240

tagattctca ggtctccagt tccctaatta tagctctgag ctgaatgtga acgccttgga 300

gctggacgac tcggccctgt atctctgtgc cagcatgaga tgatcctatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg gatgatccta ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 929

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 929

gccttgccag cccgctcagg atgatccagg cttgactttt cccacaggct cagtggagac 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctcttct cagtctgggc acaacactgt gtcctggtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attataggga ggaagagaat ggcagaggaa acttccctcc 240

tagattctca ggtctccagt tccctaatta tagctctgag ctgaatgtga acgccttgga 300

gctggacgac tcggccctgt atctctgtgc cagcatgaga tgatccaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg gatgatccag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 930

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 930

gccttgccag cccgctcagc gccaatagac actctctttt cccacaggcc cagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct atctctgggc acaagagtgt gtcctggtac caacaggtcc tgggtcaggg 180

gccccagttt atctttcagt attatgagaa agaagagaga ggaagaggaa acttccctga 240

tcgattctca gctcgccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctgggggac tcggccctgt atctctgtgc cagcatgacg ccaatagaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag cgccaataga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 931

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 931

gccttgccag cccgctcagc gccaatattc ggaacctttt cccacaggcc cagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct atctctgggc acaagagtgt gtcctggtac caacaggtcc tgggtcaggg 180

gccccagttt atctttcagt attatgagaa agaagagaga ggaagaggaa acttccctga 240

tcgattctca gctcgccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctgggggac tcggccctgt atctctgtgc cagcatgacg ccaatattcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat cgccaatatt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 932

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 932

gccttgccag cccgctcagc gccaataaag taacgctttt cccacaggcc cagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct atctctgggc acaagagtgt gtcctggtac caacaggtcc tgggtcaggg 180

gccccagttt atctttcagt attatgagaa agaagagaga ggaagaggaa acttccctga 240

tcgattctca gctcgccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctgggggac tcggccctgt atctctgtgc cagcatgacg ccaataaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag cgccaataaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 933

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 933

gccttgccag cccgctcagc gccaatagtc tcctactttt cccacaggcc cagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct atctctgggc acaagagtgt gtcctggtac caacaggtcc tgggtcaggg 180

gccccagttt atctttcagt attatgagaa agaagagaga ggaagaggaa acttccctga 240

tcgattctca gctcgccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctgggggac tcggccctgt atctctgtgc cagcatgacg ccaatagtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag cgccaatagt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 934

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 934

gccttgccag cccgctcagc gccaataaga gtgtcctttt cccacaggcc cagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct atctctgggc acaagagtgt gtcctggtac caacaggtcc tgggtcaggg 180

gccccagttt atctttcagt attatgagaa agaagagaga ggaagaggaa acttccctga 240

tcgattctca gctcgccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctgggggac tcggccctgt atctctgtgc cagcatgacg ccaataagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct cgccaataag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 935

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 935

gccttgccag cccgctcagc gccaatagtt ccgaactttt cccacaggcc cagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct atctctgggc acaagagtgt gtcctggtac caacaggtcc tgggtcaggg 180

gccccagttt atctttcagt attatgagaa agaagagaga ggaagaggaa acttccctga 240

tcgattctca gctcgccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctgggggac tcggccctgt atctctgtgc cagcatgacg ccaatagttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat cgccaatagt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 936

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 936

gccttgccag cccgctcagc gccaatacgt tacttctttt cccacaggcc cagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct atctctgggc acaagagtgt gtcctggtac caacaggtcc tgggtcaggg 180

gccccagttt atctttcagt attatgagaa agaagagaga ggaagaggaa acttccctga 240

tcgattctca gctcgccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctgggggac tcggccctgt atctctgtgc cagcatgacg ccaatacgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc cgccaatacg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 937

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 937

gccttgccag cccgctcagc gccaatatag gagacctttt cccacaggcc cagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct atctctgggc acaagagtgt gtcctggtac caacaggtcc tgggtcaggg 180

gccccagttt atctttcagt attatgagaa agaagagaga ggaagaggaa acttccctga 240

tcgattctca gctcgccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctgggggac tcggccctgt atctctgtgc cagcatgacg ccaatatagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg cgccaatata ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 938

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 938

gccttgccag cccgctcagc gccaatagtg tctacctttt cccacaggcc cagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct atctctgggc acaagagtgt gtcctggtac caacaggtcc tgggtcaggg 180

gccccagttt atctttcagt attatgagaa agaagagaga ggaagaggaa acttccctga 240

tcgattctca gctcgccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctgggggac tcggccctgt atctctgtgc cagcatgacg ccaatagtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc cgccaatagt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 939

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 939

gccttgccag cccgctcagc gccaatagta cagtgctttt cccacaggcc cagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct atctctgggc acaagagtgt gtcctggtac caacaggtcc tgggtcaggg 180

gccccagttt atctttcagt attatgagaa agaagagaga ggaagaggaa acttccctga 240

tcgattctca gctcgccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctgggggac tcggccctgt atctctgtgc cagcatgacg ccaatagtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg cgccaatagt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 940

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 940

gccttgccag cccgctcagc gccaatagga tcatcctttt cccacaggcc cagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct atctctgggc acaagagtgt gtcctggtac caacaggtcc tgggtcaggg 180

gccccagttt atctttcagt attatgagaa agaagagaga ggaagaggaa acttccctga 240

tcgattctca gctcgccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctgggggac tcggccctgt atctctgtgc cagcatgacg ccaataggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg cgccaatagg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 941

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 941

gccttgccag cccgctcagc gccaatatat tggcgctttt cccacaggcc cagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct atctctgggc acaagagtgt gtcctggtac caacaggtcc tgggtcaggg 180

gccccagttt atctttcagt attatgagaa agaagagaga ggaagaggaa acttccctga 240

tcgattctca gctcgccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctgggggac tcggccctgt atctctgtgc cagcatgacg ccaatatatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg cgccaatata ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 942

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 942

gccttgccag cccgctcagc gccaataagg cttgactttt cccacaggcc cagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct atctctgggc acaagagtgt gtcctggtac caacaggtcc tgggtcaggg 180

gccccagttt atctttcagt attatgagaa agaagagaga ggaagaggaa acttccctga 240

tcgattctca gctcgccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctgggggac tcggccctgt atctctgtgc cagcatgacg ccaataaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg cgccaataag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 943

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 943

gccttgccag cccgctcagt caagcctgac actctctttt cccacaggct tagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct aagtctgggc atgacactgt gtcctggtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attatgagga ggaagagaga cagagaggca acttccctga 240

tcgattctca ggtcaccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctgggggac tcggccctct atctctgtgc cagcatgatc aagcctgaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag tcaagcctga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 944

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 944

gccttgccag cccgctcagt caagcctttc ggaacctttt cccacaggct tagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct aagtctgggc atgacactgt gtcctggtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attatgagga ggaagagaga cagagaggca acttccctga 240

tcgattctca ggtcaccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctgggggac tcggccctct atctctgtgc cagcatgatc aagcctttcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat tcaagccttt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 945

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 945

gccttgccag cccgctcagt caagcctaag taacgctttt cccacaggct tagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct aagtctgggc atgacactgt gtcctggtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attatgagga ggaagagaga cagagaggca acttccctga 240

tcgattctca ggtcaccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctgggggac tcggccctct atctctgtgc cagcatgatc aagcctaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag tcaagcctaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 946

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 946

gccttgccag cccgctcagt caagcctgtc tcctactttt cccacaggct tagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct aagtctgggc atgacactgt gtcctggtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attatgagga ggaagagaga cagagaggca acttccctga 240

tcgattctca ggtcaccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctgggggac tcggccctct atctctgtgc cagcatgatc aagcctgtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag tcaagcctgt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 947

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 947

gccttgccag cccgctcagt caagcctaga gtgtcctttt cccacaggct tagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct aagtctgggc atgacactgt gtcctggtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attatgagga ggaagagaga cagagaggca acttccctga 240

tcgattctca ggtcaccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctgggggac tcggccctct atctctgtgc cagcatgatc aagcctagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct tcaagcctag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 948

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 948

gccttgccag cccgctcagt caagcctgtt ccgaactttt cccacaggct tagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct aagtctgggc atgacactgt gtcctggtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attatgagga ggaagagaga cagagaggca acttccctga 240

tcgattctca ggtcaccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctgggggac tcggccctct atctctgtgc cagcatgatc aagcctgttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat tcaagcctgt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 949

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 949

gccttgccag cccgctcagt caagcctcgt tacttctttt cccacaggct tagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct aagtctgggc atgacactgt gtcctggtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attatgagga ggaagagaga cagagaggca acttccctga 240

tcgattctca ggtcaccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctgggggac tcggccctct atctctgtgc cagcatgatc aagcctcgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc tcaagcctcg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 950

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 950

gccttgccag cccgctcagt caagccttag gagacctttt cccacaggct tagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct aagtctgggc atgacactgt gtcctggtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attatgagga ggaagagaga cagagaggca acttccctga 240

tcgattctca ggtcaccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctgggggac tcggccctct atctctgtgc cagcatgatc aagccttagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg tcaagcctta ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 951

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 951

gccttgccag cccgctcagt caagcctgtg tctacctttt cccacaggct tagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct aagtctgggc atgacactgt gtcctggtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attatgagga ggaagagaga cagagaggca acttccctga 240

tcgattctca ggtcaccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctgggggac tcggccctct atctctgtgc cagcatgatc aagcctgtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc tcaagcctgt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 952

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 952

gccttgccag cccgctcagt caagcctgta cagtgctttt cccacaggct tagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct aagtctgggc atgacactgt gtcctggtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attatgagga ggaagagaga cagagaggca acttccctga 240

tcgattctca ggtcaccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctgggggac tcggccctct atctctgtgc cagcatgatc aagcctgtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg tcaagcctgt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 953

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 953

gccttgccag cccgctcagt caagcctgga tcatcctttt cccacaggct tagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct aagtctgggc atgacactgt gtcctggtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attatgagga ggaagagaga cagagaggca acttccctga 240

tcgattctca ggtcaccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctgggggac tcggccctct atctctgtgc cagcatgatc aagcctggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg tcaagcctgg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 954

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 954

gccttgccag cccgctcagt caagccttat tggcgctttt cccacaggct tagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct aagtctgggc atgacactgt gtcctggtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attatgagga ggaagagaga cagagaggca acttccctga 240

tcgattctca ggtcaccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctgggggac tcggccctct atctctgtgc cagcatgatc aagccttatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg tcaagcctta ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 955

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 955

gccttgccag cccgctcagt caagcctagg cttgactttt cccacaggct tagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcaag tgactctgag 120

atgctctcct aagtctgggc atgacactgt gtcctggtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attatgagga ggaagagaga cagagaggca acttccctga 240

tcgattctca ggtcaccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctgggggac tcggccctct atctctgtgc cagcatgatc aagcctaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg tcaagcctag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 956

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 956

gccttgccag cccgctcaga cgtgtgtgac actctctttt cccacaggcc cagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcacg tgactctgag 120

atgctctcct atctctgggc acaccagtgt gtcctcgtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attatgagaa agaagagaga ggaagaggaa acttccctga 240

tcaattctca ggtcaccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctaggggac tcggccctct atctctgtgc cagcatgaac gtgtgtgaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag acgtgtgtga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 957

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 957

gccttgccag cccgctcaga cgtgtgtttc ggaacctttt cccacaggcc cagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcacg tgactctgag 120

atgctctcct atctctgggc acaccagtgt gtcctcgtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attatgagaa agaagagaga ggaagaggaa acttccctga 240

tcaattctca ggtcaccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctaggggac tcggccctct atctctgtgc cagcatgaac gtgtgtttcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat acgtgtgttt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 958

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 958

gccttgccag cccgctcaga cgtgtgtaag taacgctttt cccacaggcc cagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcacg tgactctgag 120

atgctctcct atctctgggc acaccagtgt gtcctcgtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attatgagaa agaagagaga ggaagaggaa acttccctga 240

tcaattctca ggtcaccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctaggggac tcggccctct atctctgtgc cagcatgaac gtgtgtaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag acgtgtgtaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 959

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 959

gccttgccag cccgctcaga cgtgtgtgtc tcctactttt cccacaggcc cagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcacg tgactctgag 120

atgctctcct atctctgggc acaccagtgt gtcctcgtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attatgagaa agaagagaga ggaagaggaa acttccctga 240

tcaattctca ggtcaccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctaggggac tcggccctct atctctgtgc cagcatgaac gtgtgtgtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag acgtgtgtgt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 960

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 960

gccttgccag cccgctcaga cgtgtgtaga gtgtcctttt cccacaggcc cagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcacg tgactctgag 120

atgctctcct atctctgggc acaccagtgt gtcctcgtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attatgagaa agaagagaga ggaagaggaa acttccctga 240

tcaattctca ggtcaccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctaggggac tcggccctct atctctgtgc cagcatgaac gtgtgtagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct acgtgtgtag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 961

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 961

gccttgccag cccgctcaga cgtgtgtgtt ccgaactttt cccacaggcc cagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcacg tgactctgag 120

atgctctcct atctctgggc acaccagtgt gtcctcgtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attatgagaa agaagagaga ggaagaggaa acttccctga 240

tcaattctca ggtcaccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctaggggac tcggccctct atctctgtgc cagcatgaac gtgtgtgttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat acgtgtgtgt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 962

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 962

gccttgccag cccgctcaga cgtgtgtcgt tacttctttt cccacaggcc cagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcacg tgactctgag 120

atgctctcct atctctgggc acaccagtgt gtcctcgtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attatgagaa agaagagaga ggaagaggaa acttccctga 240

tcaattctca ggtcaccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctaggggac tcggccctct atctctgtgc cagcatgaac gtgtgtcgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc acgtgtgtcg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 963

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 963

gccttgccag cccgctcaga cgtgtgttag gagacctttt cccacaggcc cagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcacg tgactctgag 120

atgctctcct atctctgggc acaccagtgt gtcctcgtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attatgagaa agaagagaga ggaagaggaa acttccctga 240

tcaattctca ggtcaccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctaggggac tcggccctct atctctgtgc cagcatgaac gtgtgttagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg acgtgtgtta ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 964

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 964

gccttgccag cccgctcaga cgtgtgtgtg tctacctttt cccacaggcc cagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcacg tgactctgag 120

atgctctcct atctctgggc acaccagtgt gtcctcgtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attatgagaa agaagagaga ggaagaggaa acttccctga 240

tcaattctca ggtcaccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctaggggac tcggccctct atctctgtgc cagcatgaac gtgtgtgtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc acgtgtgtgt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 965

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 965

gccttgccag cccgctcaga cgtgtgtgta cagtgctttt cccacaggcc cagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcacg tgactctgag 120

atgctctcct atctctgggc acaccagtgt gtcctcgtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attatgagaa agaagagaga ggaagaggaa acttccctga 240

tcaattctca ggtcaccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctaggggac tcggccctct atctctgtgc cagcatgaac gtgtgtgtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg acgtgtgtgt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 966

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 966

gccttgccag cccgctcaga cgtgtgtgga tcatcctttt cccacaggcc cagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcacg tgactctgag 120

atgctctcct atctctgggc acaccagtgt gtcctcgtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attatgagaa agaagagaga ggaagaggaa acttccctga 240

tcaattctca ggtcaccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctaggggac tcggccctct atctctgtgc cagcatgaac gtgtgtggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg acgtgtgtgg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 967

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 967

gccttgccag cccgctcaga cgtgtgttat tggcgctttt cccacaggcc cagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcacg tgactctgag 120

atgctctcct atctctgggc acaccagtgt gtcctcgtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attatgagaa agaagagaga ggaagaggaa acttccctga 240

tcaattctca ggtcaccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctaggggac tcggccctct atctctgtgc cagcatgaac gtgtgttatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg acgtgtgtta ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 968

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 968

gccttgccag cccgctcaga cgtgtgtagg cttgactttt cccacaggcc cagtggacgc 60

tggagtcacc caaagtccca cacacctgat caaaacgaga ggacagcacg tgactctgag 120

atgctctcct atctctgggc acaccagtgt gtcctcgtac caacaggccc tgggtcaggg 180

gccccagttt atctttcagt attatgagaa agaagagaga ggaagaggaa acttccctga 240

tcaattctca ggtcaccagt tccctaacta tagctctgag ctgaatgtga acgccttgtt 300

gctaggggac tcggccctct atctctgtgc cagcatgaac gtgtgtaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg acgtgtgtag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 969

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 969

gccttgccag cccgctcagt ccgtctagac actctctttt cctgcaggcc cagtggaggc 60

tggagtcaca caaagtccca cacacctgat caaaacgaga ggacagcaag cgactctgag 120

atgctctcct atctctgggc acaccagtgt gtactggtac caacaggccc tgggtctggg 180

cctccagttc ctcctttggt atgacgaggg tgaagagaga aacagaggaa acttccctcc 240

tagattttca ggtcgccagt tccctaatta tagctctgag ctgaatgtga acgccttgga 300

gctggaggac tcggccctgt atctctgtgc cagcatgatc cgtctagaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag tccgtctaga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 970

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 970

gccttgccag cccgctcagt ccgtctattc ggaacctttt cctgcaggcc cagtggaggc 60

tggagtcaca caaagtccca cacacctgat caaaacgaga ggacagcaag cgactctgag 120

atgctctcct atctctgggc acaccagtgt gtactggtac caacaggccc tgggtctggg 180

cctccagttc ctcctttggt atgacgaggg tgaagagaga aacagaggaa acttccctcc 240

tagattttca ggtcgccagt tccctaatta tagctctgag ctgaatgtga acgccttgga 300

gctggaggac tcggccctgt atctctgtgc cagcatgatc cgtctattcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat tccgtctatt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 971

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 971

gccttgccag cccgctcagt ccgtctaaag taacgctttt cctgcaggcc cagtggaggc 60

tggagtcaca caaagtccca cacacctgat caaaacgaga ggacagcaag cgactctgag 120

atgctctcct atctctgggc acaccagtgt gtactggtac caacaggccc tgggtctggg 180

cctccagttc ctcctttggt atgacgaggg tgaagagaga aacagaggaa acttccctcc 240

tagattttca ggtcgccagt tccctaatta tagctctgag ctgaatgtga acgccttgga 300

gctggaggac tcggccctgt atctctgtgc cagcatgatc cgtctaaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag tccgtctaaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 972

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 972

gccttgccag cccgctcagt ccgtctagtc tcctactttt cctgcaggcc cagtggaggc 60

tggagtcaca caaagtccca cacacctgat caaaacgaga ggacagcaag cgactctgag 120

atgctctcct atctctgggc acaccagtgt gtactggtac caacaggccc tgggtctggg 180

cctccagttc ctcctttggt atgacgaggg tgaagagaga aacagaggaa acttccctcc 240

tagattttca ggtcgccagt tccctaatta tagctctgag ctgaatgtga acgccttgga 300

gctggaggac tcggccctgt atctctgtgc cagcatgatc cgtctagtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag tccgtctagt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 973

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 973

gccttgccag cccgctcagt ccgtctaaga gtgtcctttt cctgcaggcc cagtggaggc 60

tggagtcaca caaagtccca cacacctgat caaaacgaga ggacagcaag cgactctgag 120

atgctctcct atctctgggc acaccagtgt gtactggtac caacaggccc tgggtctggg 180

cctccagttc ctcctttggt atgacgaggg tgaagagaga aacagaggaa acttccctcc 240

tagattttca ggtcgccagt tccctaatta tagctctgag ctgaatgtga acgccttgga 300

gctggaggac tcggccctgt atctctgtgc cagcatgatc cgtctaagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct tccgtctaag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 974

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 974

gccttgccag cccgctcagt ccgtctagtt ccgaactttt cctgcaggcc cagtggaggc 60

tggagtcaca caaagtccca cacacctgat caaaacgaga ggacagcaag cgactctgag 120

atgctctcct atctctgggc acaccagtgt gtactggtac caacaggccc tgggtctggg 180

cctccagttc ctcctttggt atgacgaggg tgaagagaga aacagaggaa acttccctcc 240

tagattttca ggtcgccagt tccctaatta tagctctgag ctgaatgtga acgccttgga 300

gctggaggac tcggccctgt atctctgtgc cagcatgatc cgtctagttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat tccgtctagt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 975

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 975

gccttgccag cccgctcagt ccgtctacgt tacttctttt cctgcaggcc cagtggaggc 60

tggagtcaca caaagtccca cacacctgat caaaacgaga ggacagcaag cgactctgag 120

atgctctcct atctctgggc acaccagtgt gtactggtac caacaggccc tgggtctggg 180

cctccagttc ctcctttggt atgacgaggg tgaagagaga aacagaggaa acttccctcc 240

tagattttca ggtcgccagt tccctaatta tagctctgag ctgaatgtga acgccttgga 300

gctggaggac tcggccctgt atctctgtgc cagcatgatc cgtctacgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc tccgtctacg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 976

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 976

gccttgccag cccgctcagt ccgtctatag gagacctttt cctgcaggcc cagtggaggc 60

tggagtcaca caaagtccca cacacctgat caaaacgaga ggacagcaag cgactctgag 120

atgctctcct atctctgggc acaccagtgt gtactggtac caacaggccc tgggtctggg 180

cctccagttc ctcctttggt atgacgaggg tgaagagaga aacagaggaa acttccctcc 240

tagattttca ggtcgccagt tccctaatta tagctctgag ctgaatgtga acgccttgga 300

gctggaggac tcggccctgt atctctgtgc cagcatgatc cgtctatagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg tccgtctata ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 977

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 977

gccttgccag cccgctcagt ccgtctagtg tctacctttt cctgcaggcc cagtggaggc 60

tggagtcaca caaagtccca cacacctgat caaaacgaga ggacagcaag cgactctgag 120

atgctctcct atctctgggc acaccagtgt gtactggtac caacaggccc tgggtctggg 180

cctccagttc ctcctttggt atgacgaggg tgaagagaga aacagaggaa acttccctcc 240

tagattttca ggtcgccagt tccctaatta tagctctgag ctgaatgtga acgccttgga 300

gctggaggac tcggccctgt atctctgtgc cagcatgatc cgtctagtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc tccgtctagt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 978

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 978

gccttgccag cccgctcagt ccgtctagta cagtgctttt cctgcaggcc cagtggaggc 60

tggagtcaca caaagtccca cacacctgat caaaacgaga ggacagcaag cgactctgag 120

atgctctcct atctctgggc acaccagtgt gtactggtac caacaggccc tgggtctggg 180

cctccagttc ctcctttggt atgacgaggg tgaagagaga aacagaggaa acttccctcc 240

tagattttca ggtcgccagt tccctaatta tagctctgag ctgaatgtga acgccttgga 300

gctggaggac tcggccctgt atctctgtgc cagcatgatc cgtctagtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg tccgtctagt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 979

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 979

gccttgccag cccgctcagt ccgtctagga tcatcctttt cctgcaggcc cagtggaggc 60

tggagtcaca caaagtccca cacacctgat caaaacgaga ggacagcaag cgactctgag 120

atgctctcct atctctgggc acaccagtgt gtactggtac caacaggccc tgggtctggg 180

cctccagttc ctcctttggt atgacgaggg tgaagagaga aacagaggaa acttccctcc 240

tagattttca ggtcgccagt tccctaatta tagctctgag ctgaatgtga acgccttgga 300

gctggaggac tcggccctgt atctctgtgc cagcatgatc cgtctaggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg tccgtctagg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 980

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 980

gccttgccag cccgctcagt ccgtctatat tggcgctttt cctgcaggcc cagtggaggc 60

tggagtcaca caaagtccca cacacctgat caaaacgaga ggacagcaag cgactctgag 120

atgctctcct atctctgggc acaccagtgt gtactggtac caacaggccc tgggtctggg 180

cctccagttc ctcctttggt atgacgaggg tgaagagaga aacagaggaa acttccctcc 240

tagattttca ggtcgccagt tccctaatta tagctctgag ctgaatgtga acgccttgga 300

gctggaggac tcggccctgt atctctgtgc cagcatgatc cgtctatatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg tccgtctata ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 981

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 981

gccttgccag cccgctcagt ccgtctaagg cttgactttt cctgcaggcc cagtggaggc 60

tggagtcaca caaagtccca cacacctgat caaaacgaga ggacagcaag cgactctgag 120

atgctctcct atctctgggc acaccagtgt gtactggtac caacaggccc tgggtctggg 180

cctccagttc ctcctttggt atgacgaggg tgaagagaga aacagaggaa acttccctcc 240

tagattttca ggtcgccagt tccctaatta tagctctgag ctgaatgtga acgccttgga 300

gctggaggac tcggccctgt atctctgtgc cagcatgatc cgtctaaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg tccgtctaag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 982

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 982

gccttgccag cccgctcaga agagctggac actctcttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccaggtc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca taactccatg tactggtatc gacaagaccc aggcatggga 180

ctgaggctga tttattactc agcttctgag ggtaccactg acaaaggaga agtccccaat 240

ggctacaatg tctccagatt aaacaaacgg gagttctcgc tcaggctgga gtcggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgaaa gagctggaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag aagagctgga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 983

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 983

gccttgccag cccgctcaga agagctgttc ggaaccttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccaggtc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca taactccatg tactggtatc gacaagaccc aggcatggga 180

ctgaggctga tttattactc agcttctgag ggtaccactg acaaaggaga agtccccaat 240

ggctacaatg tctccagatt aaacaaacgg gagttctcgc tcaggctgga gtcggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgaaa gagctgttcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat aagagctgtt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 984

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 984

gccttgccag cccgctcaga agagctgaag taacgcttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccaggtc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca taactccatg tactggtatc gacaagaccc aggcatggga 180

ctgaggctga tttattactc agcttctgag ggtaccactg acaaaggaga agtccccaat 240

ggctacaatg tctccagatt aaacaaacgg gagttctcgc tcaggctgga gtcggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgaaa gagctgaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag aagagctgaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 985

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 985

gccttgccag cccgctcaga agagctggtc tcctacttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccaggtc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca taactccatg tactggtatc gacaagaccc aggcatggga 180

ctgaggctga tttattactc agcttctgag ggtaccactg acaaaggaga agtccccaat 240

ggctacaatg tctccagatt aaacaaacgg gagttctcgc tcaggctgga gtcggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgaaa gagctggtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag aagagctggt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 986

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 986

gccttgccag cccgctcaga agagctgaga gtgtccttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccaggtc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca taactccatg tactggtatc gacaagaccc aggcatggga 180

ctgaggctga tttattactc agcttctgag ggtaccactg acaaaggaga agtccccaat 240

ggctacaatg tctccagatt aaacaaacgg gagttctcgc tcaggctgga gtcggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgaaa gagctgagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct aagagctgag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 987

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 987

gccttgccag cccgctcaga agagctggtt ccgaacttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccaggtc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca taactccatg tactggtatc gacaagaccc aggcatggga 180

ctgaggctga tttattactc agcttctgag ggtaccactg acaaaggaga agtccccaat 240

ggctacaatg tctccagatt aaacaaacgg gagttctcgc tcaggctgga gtcggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgaaa gagctggttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat aagagctggt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 988

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 988

gccttgccag cccgctcaga agagctgcgt tacttcttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccaggtc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca taactccatg tactggtatc gacaagaccc aggcatggga 180

ctgaggctga tttattactc agcttctgag ggtaccactg acaaaggaga agtccccaat 240

ggctacaatg tctccagatt aaacaaacgg gagttctcgc tcaggctgga gtcggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgaaa gagctgcgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc aagagctgcg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 989

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 989

gccttgccag cccgctcaga agagctgtag gagaccttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccaggtc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca taactccatg tactggtatc gacaagaccc aggcatggga 180

ctgaggctga tttattactc agcttctgag ggtaccactg acaaaggaga agtccccaat 240

ggctacaatg tctccagatt aaacaaacgg gagttctcgc tcaggctgga gtcggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgaaa gagctgtagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg aagagctgta ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 990

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 990

gccttgccag cccgctcaga agagctggtg tctaccttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccaggtc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca taactccatg tactggtatc gacaagaccc aggcatggga 180

ctgaggctga tttattactc agcttctgag ggtaccactg acaaaggaga agtccccaat 240

ggctacaatg tctccagatt aaacaaacgg gagttctcgc tcaggctgga gtcggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgaaa gagctggtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc aagagctggt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 991

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 991

gccttgccag cccgctcaga agagctggta cagtgcttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccaggtc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca taactccatg tactggtatc gacaagaccc aggcatggga 180

ctgaggctga tttattactc agcttctgag ggtaccactg acaaaggaga agtccccaat 240

ggctacaatg tctccagatt aaacaaacgg gagttctcgc tcaggctgga gtcggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgaaa gagctggtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg aagagctggt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 992

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 992

gccttgccag cccgctcaga agagctggga tcatccttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccaggtc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca taactccatg tactggtatc gacaagaccc aggcatggga 180

ctgaggctga tttattactc agcttctgag ggtaccactg acaaaggaga agtccccaat 240

ggctacaatg tctccagatt aaacaaacgg gagttctcgc tcaggctgga gtcggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgaaa gagctgggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg aagagctggg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 993

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 993

gccttgccag cccgctcaga agagctgtat tggcgcttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccaggtc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca taactccatg tactggtatc gacaagaccc aggcatggga 180

ctgaggctga tttattactc agcttctgag ggtaccactg acaaaggaga agtccccaat 240

ggctacaatg tctccagatt aaacaaacgg gagttctcgc tcaggctgga gtcggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgaaa gagctgtatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg aagagctgta ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 994

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 994

gccttgccag cccgctcaga agagctgagg cttgacttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccaggtc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca taactccatg tactggtatc gacaagaccc aggcatggga 180

ctgaggctga tttattactc agcttctgag ggtaccactg acaaaggaga agtccccaat 240

ggctacaatg tctccagatt aaacaaacgg gagttctcgc tcaggctgga gtcggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgaaa gagctgaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg aagagctgag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 995

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 995

gccttgccag cccgctcagt cagatgcgac actctcttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccgggtc ctgaagacag gacagagcat gacactgctg 120

tgtgcccagg atatgaacca tgaatacatg tactggtatc gacaagaccc aggcatgggg 180

ctgaggctga ttcattactc agttggtgag ggtacaactg ccaaaggaga ggtccctgat 240

ggctacaatg tctccagatt aaaaaaacag aatttcctgc tggggttgga gtcggctgct 300

ccctcccaaa catctgtgta cttctgtgcc agcagtgatc agatgcgaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag tcagatgcga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 996

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 996

gccttgccag cccgctcagt cagatgcttc ggaaccttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccgggtc ctgaagacag gacagagcat gacactgctg 120

tgtgcccagg atatgaacca tgaatacatg tactggtatc gacaagaccc aggcatgggg 180

ctgaggctga ttcattactc agttggtgag ggtacaactg ccaaaggaga ggtccctgat 240

ggctacaatg tctccagatt aaaaaaacag aatttcctgc tggggttgga gtcggctgct 300

ccctcccaaa catctgtgta cttctgtgcc agcagtgatc agatgcttcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat tcagatgctt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 997

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 997

gccttgccag cccgctcagt cagatgcaag taacgcttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccgggtc ctgaagacag gacagagcat gacactgctg 120

tgtgcccagg atatgaacca tgaatacatg tactggtatc gacaagaccc aggcatgggg 180

ctgaggctga ttcattactc agttggtgag ggtacaactg ccaaaggaga ggtccctgat 240

ggctacaatg tctccagatt aaaaaaacag aatttcctgc tggggttgga gtcggctgct 300

ccctcccaaa catctgtgta cttctgtgcc agcagtgatc agatgcaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag tcagatgcaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 998

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 998

gccttgccag cccgctcagt cagatgcgtc tcctacttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccgggtc ctgaagacag gacagagcat gacactgctg 120

tgtgcccagg atatgaacca tgaatacatg tactggtatc gacaagaccc aggcatgggg 180

ctgaggctga ttcattactc agttggtgag ggtacaactg ccaaaggaga ggtccctgat 240

ggctacaatg tctccagatt aaaaaaacag aatttcctgc tggggttgga gtcggctgct 300

ccctcccaaa catctgtgta cttctgtgcc agcagtgatc agatgcgtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag tcagatgcgt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 999

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 999

gccttgccag cccgctcagt cagatgcaga gtgtccttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccgggtc ctgaagacag gacagagcat gacactgctg 120

tgtgcccagg atatgaacca tgaatacatg tactggtatc gacaagaccc aggcatgggg 180

ctgaggctga ttcattactc agttggtgag ggtacaactg ccaaaggaga ggtccctgat 240

ggctacaatg tctccagatt aaaaaaacag aatttcctgc tggggttgga gtcggctgct 300

ccctcccaaa catctgtgta cttctgtgcc agcagtgatc agatgcagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct tcagatgcag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1000

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1000

gccttgccag cccgctcagt cagatgcgtt ccgaacttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccgggtc ctgaagacag gacagagcat gacactgctg 120

tgtgcccagg atatgaacca tgaatacatg tactggtatc gacaagaccc aggcatgggg 180

ctgaggctga ttcattactc agttggtgag ggtacaactg ccaaaggaga ggtccctgat 240

ggctacaatg tctccagatt aaaaaaacag aatttcctgc tggggttgga gtcggctgct 300

ccctcccaaa catctgtgta cttctgtgcc agcagtgatc agatgcgttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat tcagatgcgt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1001

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1001

gccttgccag cccgctcagt cagatgccgt tacttcttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccgggtc ctgaagacag gacagagcat gacactgctg 120

tgtgcccagg atatgaacca tgaatacatg tactggtatc gacaagaccc aggcatgggg 180

ctgaggctga ttcattactc agttggtgag ggtacaactg ccaaaggaga ggtccctgat 240

ggctacaatg tctccagatt aaaaaaacag aatttcctgc tggggttgga gtcggctgct 300

ccctcccaaa catctgtgta cttctgtgcc agcagtgatc agatgccgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc tcagatgccg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1002

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1002

gccttgccag cccgctcagt cagatgctag gagaccttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccgggtc ctgaagacag gacagagcat gacactgctg 120

tgtgcccagg atatgaacca tgaatacatg tactggtatc gacaagaccc aggcatgggg 180

ctgaggctga ttcattactc agttggtgag ggtacaactg ccaaaggaga ggtccctgat 240

ggctacaatg tctccagatt aaaaaaacag aatttcctgc tggggttgga gtcggctgct 300

ccctcccaaa catctgtgta cttctgtgcc agcagtgatc agatgctagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg tcagatgcta ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1003

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1003

gccttgccag cccgctcagt cagatgcgtg tctaccttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccgggtc ctgaagacag gacagagcat gacactgctg 120

tgtgcccagg atatgaacca tgaatacatg tactggtatc gacaagaccc aggcatgggg 180

ctgaggctga ttcattactc agttggtgag ggtacaactg ccaaaggaga ggtccctgat 240

ggctacaatg tctccagatt aaaaaaacag aatttcctgc tggggttgga gtcggctgct 300

ccctcccaaa catctgtgta cttctgtgcc agcagtgatc agatgcgtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc tcagatgcgt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1004

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1004

gccttgccag cccgctcagt cagatgcgta cagtgcttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccgggtc ctgaagacag gacagagcat gacactgctg 120

tgtgcccagg atatgaacca tgaatacatg tactggtatc gacaagaccc aggcatgggg 180

ctgaggctga ttcattactc agttggtgag ggtacaactg ccaaaggaga ggtccctgat 240

ggctacaatg tctccagatt aaaaaaacag aatttcctgc tggggttgga gtcggctgct 300

ccctcccaaa catctgtgta cttctgtgcc agcagtgatc agatgcgtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg tcagatgcgt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1005

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1005

gccttgccag cccgctcagt cagatgcgga tcatccttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccgggtc ctgaagacag gacagagcat gacactgctg 120

tgtgcccagg atatgaacca tgaatacatg tactggtatc gacaagaccc aggcatgggg 180

ctgaggctga ttcattactc agttggtgag ggtacaactg ccaaaggaga ggtccctgat 240

ggctacaatg tctccagatt aaaaaaacag aatttcctgc tggggttgga gtcggctgct 300

ccctcccaaa catctgtgta cttctgtgcc agcagtgatc agatgcggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg tcagatgcgg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1006

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1006

gccttgccag cccgctcagt cagatgctat tggcgcttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccgggtc ctgaagacag gacagagcat gacactgctg 120

tgtgcccagg atatgaacca tgaatacatg tactggtatc gacaagaccc aggcatgggg 180

ctgaggctga ttcattactc agttggtgag ggtacaactg ccaaaggaga ggtccctgat 240

ggctacaatg tctccagatt aaaaaaacag aatttcctgc tggggttgga gtcggctgct 300

ccctcccaaa catctgtgta cttctgtgcc agcagtgatc agatgctatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg tcagatgcta ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1007

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1007

gccttgccag cccgctcagt cagatgcagg cttgacttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccgggtc ctgaagacag gacagagcat gacactgctg 120

tgtgcccagg atatgaacca tgaatacatg tactggtatc gacaagaccc aggcatgggg 180

ctgaggctga ttcattactc agttggtgag ggtacaactg ccaaaggaga ggtccctgat 240

ggctacaatg tctccagatt aaaaaaacag aatttcctgc tggggttgga gtcggctgct 300

ccctcccaaa catctgtgta cttctgtgcc agcagtgatc agatgcaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg tcagatgcag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1008

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1008

gccttgccag cccgctcagg tgtagcagac actctcttct ctgtaggtcc agtgattgct 60

gggatcaccc aggcaccaac atctcagatc ctggcagcag gacggcgcat gacactgaga 120

tgtacccagg atatgagaca taatgccatg tactggtata gacaagatct aggactgggg 180

ctaaggctca tccattattc aaatactgca ggtaccactg gcaaaggaga agtccctgat 240

ggttatagtg tctccagagc aaacacagat gatttccccc tcacgttggc gtctgctgta 300

ccctctcaga catctgtgta cttctgtgcc agcagtgagt gtagcagaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag gtgtagcaga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1009

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1009

gccttgccag cccgctcagg tgtagcattc ggaaccttct ctgtaggtcc agtgattgct 60

gggatcaccc aggcaccaac atctcagatc ctggcagcag gacggcgcat gacactgaga 120

tgtacccagg atatgagaca taatgccatg tactggtata gacaagatct aggactgggg 180

ctaaggctca tccattattc aaatactgca ggtaccactg gcaaaggaga agtccctgat 240

ggttatagtg tctccagagc aaacacagat gatttccccc tcacgttggc gtctgctgta 300

ccctctcaga catctgtgta cttctgtgcc agcagtgagt gtagcattcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat gtgtagcatt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1010

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1010

gccttgccag cccgctcagg tgtagcaaag taacgcttct ctgtaggtcc agtgattgct 60

gggatcaccc aggcaccaac atctcagatc ctggcagcag gacggcgcat gacactgaga 120

tgtacccagg atatgagaca taatgccatg tactggtata gacaagatct aggactgggg 180

ctaaggctca tccattattc aaatactgca ggtaccactg gcaaaggaga agtccctgat 240

ggttatagtg tctccagagc aaacacagat gatttccccc tcacgttggc gtctgctgta 300

ccctctcaga catctgtgta cttctgtgcc agcagtgagt gtagcaaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag gtgtagcaaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1011

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1011

gccttgccag cccgctcagg tgtagcagtc tcctacttct ctgtaggtcc agtgattgct 60

gggatcaccc aggcaccaac atctcagatc ctggcagcag gacggcgcat gacactgaga 120

tgtacccagg atatgagaca taatgccatg tactggtata gacaagatct aggactgggg 180

ctaaggctca tccattattc aaatactgca ggtaccactg gcaaaggaga agtccctgat 240

ggttatagtg tctccagagc aaacacagat gatttccccc tcacgttggc gtctgctgta 300

ccctctcaga catctgtgta cttctgtgcc agcagtgagt gtagcagtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag gtgtagcagt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1012

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1012

gccttgccag cccgctcagg tgtagcaaga gtgtccttct ctgtaggtcc agtgattgct 60

gggatcaccc aggcaccaac atctcagatc ctggcagcag gacggcgcat gacactgaga 120

tgtacccagg atatgagaca taatgccatg tactggtata gacaagatct aggactgggg 180

ctaaggctca tccattattc aaatactgca ggtaccactg gcaaaggaga agtccctgat 240

ggttatagtg tctccagagc aaacacagat gatttccccc tcacgttggc gtctgctgta 300

ccctctcaga catctgtgta cttctgtgcc agcagtgagt gtagcaagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct gtgtagcaag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1013

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1013

gccttgccag cccgctcagg tgtagcagtt ccgaacttct ctgtaggtcc agtgattgct 60

gggatcaccc aggcaccaac atctcagatc ctggcagcag gacggcgcat gacactgaga 120

tgtacccagg atatgagaca taatgccatg tactggtata gacaagatct aggactgggg 180

ctaaggctca tccattattc aaatactgca ggtaccactg gcaaaggaga agtccctgat 240

ggttatagtg tctccagagc aaacacagat gatttccccc tcacgttggc gtctgctgta 300

ccctctcaga catctgtgta cttctgtgcc agcagtgagt gtagcagttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat gtgtagcagt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1014

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1014

gccttgccag cccgctcagg tgtagcacgt tacttcttct ctgtaggtcc agtgattgct 60

gggatcaccc aggcaccaac atctcagatc ctggcagcag gacggcgcat gacactgaga 120

tgtacccagg atatgagaca taatgccatg tactggtata gacaagatct aggactgggg 180

ctaaggctca tccattattc aaatactgca ggtaccactg gcaaaggaga agtccctgat 240

ggttatagtg tctccagagc aaacacagat gatttccccc tcacgttggc gtctgctgta 300

ccctctcaga catctgtgta cttctgtgcc agcagtgagt gtagcacgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc gtgtagcacg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1015

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1015

gccttgccag cccgctcagg tgtagcatag gagaccttct ctgtaggtcc agtgattgct 60

gggatcaccc aggcaccaac atctcagatc ctggcagcag gacggcgcat gacactgaga 120

tgtacccagg atatgagaca taatgccatg tactggtata gacaagatct aggactgggg 180

ctaaggctca tccattattc aaatactgca ggtaccactg gcaaaggaga agtccctgat 240

ggttatagtg tctccagagc aaacacagat gatttccccc tcacgttggc gtctgctgta 300

ccctctcaga catctgtgta cttctgtgcc agcagtgagt gtagcatagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg gtgtagcata ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1016

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1016

gccttgccag cccgctcagg tgtagcagtg tctaccttct ctgtaggtcc agtgattgct 60

gggatcaccc aggcaccaac atctcagatc ctggcagcag gacggcgcat gacactgaga 120

tgtacccagg atatgagaca taatgccatg tactggtata gacaagatct aggactgggg 180

ctaaggctca tccattattc aaatactgca ggtaccactg gcaaaggaga agtccctgat 240

ggttatagtg tctccagagc aaacacagat gatttccccc tcacgttggc gtctgctgta 300

ccctctcaga catctgtgta cttctgtgcc agcagtgagt gtagcagtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc gtgtagcagt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1017

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1017

gccttgccag cccgctcagg tgtagcagta cagtgcttct ctgtaggtcc agtgattgct 60

gggatcaccc aggcaccaac atctcagatc ctggcagcag gacggcgcat gacactgaga 120

tgtacccagg atatgagaca taatgccatg tactggtata gacaagatct aggactgggg 180

ctaaggctca tccattattc aaatactgca ggtaccactg gcaaaggaga agtccctgat 240

ggttatagtg tctccagagc aaacacagat gatttccccc tcacgttggc gtctgctgta 300

ccctctcaga catctgtgta cttctgtgcc agcagtgagt gtagcagtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg gtgtagcagt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1018

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1018

gccttgccag cccgctcagg tgtagcagga tcatccttct ctgtaggtcc agtgattgct 60

gggatcaccc aggcaccaac atctcagatc ctggcagcag gacggcgcat gacactgaga 120

tgtacccagg atatgagaca taatgccatg tactggtata gacaagatct aggactgggg 180

ctaaggctca tccattattc aaatactgca ggtaccactg gcaaaggaga agtccctgat 240

ggttatagtg tctccagagc aaacacagat gatttccccc tcacgttggc gtctgctgta 300

ccctctcaga catctgtgta cttctgtgcc agcagtgagt gtagcaggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg gtgtagcagg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1019

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1019

gccttgccag cccgctcagg tgtagcatat tggcgcttct ctgtaggtcc agtgattgct 60

gggatcaccc aggcaccaac atctcagatc ctggcagcag gacggcgcat gacactgaga 120

tgtacccagg atatgagaca taatgccatg tactggtata gacaagatct aggactgggg 180

ctaaggctca tccattattc aaatactgca ggtaccactg gcaaaggaga agtccctgat 240

ggttatagtg tctccagagc aaacacagat gatttccccc tcacgttggc gtctgctgta 300

ccctctcaga catctgtgta cttctgtgcc agcagtgagt gtagcatatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg gtgtagcata ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1020

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1020

gccttgccag cccgctcagg tgtagcaagg cttgacttct ctgtaggtcc agtgattgct 60

gggatcaccc aggcaccaac atctcagatc ctggcagcag gacggcgcat gacactgaga 120

tgtacccagg atatgagaca taatgccatg tactggtata gacaagatct aggactgggg 180

ctaaggctca tccattattc aaatactgca ggtaccactg gcaaaggaga agtccctgat 240

ggttatagtg tctccagagc aaacacagat gatttccccc tcacgttggc gtctgctgta 300

ccctctcaga catctgtgta cttctgtgcc agcagtgagt gtagcaaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg gtgtagcaag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1021

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1021

gccttgccag cccgctcagt ggcagttgac actctcttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccaggtc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca tgaatacatg tcctggtatc gacaagaccc aggcatgggg 180

ctgaggctga ttcattactc agttggtgct ggtatcactg accaaggaga agtccccaat 240

ggctacaatg tctccagatc aaccacagag gatttcccgc tcaggctgct gtcggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgatg gcagttgaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag tggcagttga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1022

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1022

gccttgccag cccgctcagt ggcagttttc ggaaccttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccaggtc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca tgaatacatg tcctggtatc gacaagaccc aggcatgggg 180

ctgaggctga ttcattactc agttggtgct ggtatcactg accaaggaga agtccccaat 240

ggctacaatg tctccagatc aaccacagag gatttcccgc tcaggctgct gtcggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgatg gcagttttcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat tggcagtttt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1023

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1023

gccttgccag cccgctcagt ggcagttaag taacgcttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccaggtc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca tgaatacatg tcctggtatc gacaagaccc aggcatgggg 180

ctgaggctga ttcattactc agttggtgct ggtatcactg accaaggaga agtccccaat 240

ggctacaatg tctccagatc aaccacagag gatttcccgc tcaggctgct gtcggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgatg gcagttaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag tggcagttaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1024

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1024

gccttgccag cccgctcagt ggcagttgtc tcctacttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccaggtc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca tgaatacatg tcctggtatc gacaagaccc aggcatgggg 180

ctgaggctga ttcattactc agttggtgct ggtatcactg accaaggaga agtccccaat 240

ggctacaatg tctccagatc aaccacagag gatttcccgc tcaggctgct gtcggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgatg gcagttgtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag tggcagttgt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1025

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1025

gccttgccag cccgctcagt ggcagttaga gtgtccttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccaggtc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca tgaatacatg tcctggtatc gacaagaccc aggcatgggg 180

ctgaggctga ttcattactc agttggtgct ggtatcactg accaaggaga agtccccaat 240

ggctacaatg tctccagatc aaccacagag gatttcccgc tcaggctgct gtcggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgatg gcagttagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct tggcagttag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1026

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1026

gccttgccag cccgctcagt ggcagttgtt ccgaacttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccaggtc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca tgaatacatg tcctggtatc gacaagaccc aggcatgggg 180

ctgaggctga ttcattactc agttggtgct ggtatcactg accaaggaga agtccccaat 240

ggctacaatg tctccagatc aaccacagag gatttcccgc tcaggctgct gtcggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgatg gcagttgttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat tggcagttgt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1027

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1027

gccttgccag cccgctcagt ggcagttcgt tacttcttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccaggtc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca tgaatacatg tcctggtatc gacaagaccc aggcatgggg 180

ctgaggctga ttcattactc agttggtgct ggtatcactg accaaggaga agtccccaat 240

ggctacaatg tctccagatc aaccacagag gatttcccgc tcaggctgct gtcggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgatg gcagttcgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc tggcagttcg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1028

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1028

gccttgccag cccgctcagt ggcagtttag gagaccttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccaggtc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca tgaatacatg tcctggtatc gacaagaccc aggcatgggg 180

ctgaggctga ttcattactc agttggtgct ggtatcactg accaaggaga agtccccaat 240

ggctacaatg tctccagatc aaccacagag gatttcccgc tcaggctgct gtcggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgatg gcagtttagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg tggcagttta ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1029

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1029

gccttgccag cccgctcagt ggcagttgtg tctaccttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccaggtc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca tgaatacatg tcctggtatc gacaagaccc aggcatgggg 180

ctgaggctga ttcattactc agttggtgct ggtatcactg accaaggaga agtccccaat 240

ggctacaatg tctccagatc aaccacagag gatttcccgc tcaggctgct gtcggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgatg gcagttgtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc tggcagttgt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1030

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1030

gccttgccag cccgctcagt ggcagttgta cagtgcttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccaggtc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca tgaatacatg tcctggtatc gacaagaccc aggcatgggg 180

ctgaggctga ttcattactc agttggtgct ggtatcactg accaaggaga agtccccaat 240

ggctacaatg tctccagatc aaccacagag gatttcccgc tcaggctgct gtcggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgatg gcagttgtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg tggcagttgt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1031

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1031

gccttgccag cccgctcagt ggcagttgga tcatccttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccaggtc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca tgaatacatg tcctggtatc gacaagaccc aggcatgggg 180

ctgaggctga ttcattactc agttggtgct ggtatcactg accaaggaga agtccccaat 240

ggctacaatg tctccagatc aaccacagag gatttcccgc tcaggctgct gtcggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgatg gcagttggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg tggcagttgg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1032

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1032

gccttgccag cccgctcagt ggcagtttat tggcgcttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccaggtc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca tgaatacatg tcctggtatc gacaagaccc aggcatgggg 180

ctgaggctga ttcattactc agttggtgct ggtatcactg accaaggaga agtccccaat 240

ggctacaatg tctccagatc aaccacagag gatttcccgc tcaggctgct gtcggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgatg gcagtttatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg tggcagttta ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1033

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1033

gccttgccag cccgctcagt ggcagttagg cttgacttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccaggtc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca tgaatacatg tcctggtatc gacaagaccc aggcatgggg 180

ctgaggctga ttcattactc agttggtgct ggtatcactg accaaggaga agtccccaat 240

ggctacaatg tctccagatc aaccacagag gatttcccgc tcaggctgct gtcggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgatg gcagttaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg tggcagttag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1034

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1034

gccttgccag cccgctcagc agtccaagac actctcttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccgcatc ctgaagatag gacagagcat gacactgcag 120

tgtacccagg atatgaacca taactacatg tactggtatc gacaagaccc aggcatgggg 180

ctgaagctga tttattattc agttggtgct ggtatcactg ataaaggaga agtcccgaat 240

ggctacaacg tctccagatc aaccacagag gatttcccgc tcaggctgga gttggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgaca gtccaagaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag cagtccaaga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1035

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1035

gccttgccag cccgctcagc agtccaattc ggaaccttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccgcatc ctgaagatag gacagagcat gacactgcag 120

tgtacccagg atatgaacca taactacatg tactggtatc gacaagaccc aggcatgggg 180

ctgaagctga tttattattc agttggtgct ggtatcactg ataaaggaga agtcccgaat 240

ggctacaacg tctccagatc aaccacagag gatttcccgc tcaggctgga gttggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgaca gtccaattcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat cagtccaatt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1036

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1036

gccttgccag cccgctcagc agtccaaaag taacgcttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccgcatc ctgaagatag gacagagcat gacactgcag 120

tgtacccagg atatgaacca taactacatg tactggtatc gacaagaccc aggcatgggg 180

ctgaagctga tttattattc agttggtgct ggtatcactg ataaaggaga agtcccgaat 240

ggctacaacg tctccagatc aaccacagag gatttcccgc tcaggctgga gttggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgaca gtccaaaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag cagtccaaaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1037

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1037

gccttgccag cccgctcagc agtccaagtc tcctacttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccgcatc ctgaagatag gacagagcat gacactgcag 120

tgtacccagg atatgaacca taactacatg tactggtatc gacaagaccc aggcatgggg 180

ctgaagctga tttattattc agttggtgct ggtatcactg ataaaggaga agtcccgaat 240

ggctacaacg tctccagatc aaccacagag gatttcccgc tcaggctgga gttggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgaca gtccaagtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag cagtccaagt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1038

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1038

gccttgccag cccgctcagc agtccaaaga gtgtccttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccgcatc ctgaagatag gacagagcat gacactgcag 120

tgtacccagg atatgaacca taactacatg tactggtatc gacaagaccc aggcatgggg 180

ctgaagctga tttattattc agttggtgct ggtatcactg ataaaggaga agtcccgaat 240

ggctacaacg tctccagatc aaccacagag gatttcccgc tcaggctgga gttggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgaca gtccaaagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct cagtccaaag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1039

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1039

gccttgccag cccgctcagc agtccaagtt ccgaacttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccgcatc ctgaagatag gacagagcat gacactgcag 120

tgtacccagg atatgaacca taactacatg tactggtatc gacaagaccc aggcatgggg 180

ctgaagctga tttattattc agttggtgct ggtatcactg ataaaggaga agtcccgaat 240

ggctacaacg tctccagatc aaccacagag gatttcccgc tcaggctgga gttggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgaca gtccaagttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat cagtccaagt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1040

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1040

gccttgccag cccgctcagc agtccaacgt tacttcttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccgcatc ctgaagatag gacagagcat gacactgcag 120

tgtacccagg atatgaacca taactacatg tactggtatc gacaagaccc aggcatgggg 180

ctgaagctga tttattattc agttggtgct ggtatcactg ataaaggaga agtcccgaat 240

ggctacaacg tctccagatc aaccacagag gatttcccgc tcaggctgga gttggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgaca gtccaacgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc cagtccaacg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1041

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1041

gccttgccag cccgctcagc agtccaatag gagaccttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccgcatc ctgaagatag gacagagcat gacactgcag 120

tgtacccagg atatgaacca taactacatg tactggtatc gacaagaccc aggcatgggg 180

ctgaagctga tttattattc agttggtgct ggtatcactg ataaaggaga agtcccgaat 240

ggctacaacg tctccagatc aaccacagag gatttcccgc tcaggctgga gttggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgaca gtccaatagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg cagtccaata ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1042

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1042

gccttgccag cccgctcagc agtccaagtg tctaccttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccgcatc ctgaagatag gacagagcat gacactgcag 120

tgtacccagg atatgaacca taactacatg tactggtatc gacaagaccc aggcatgggg 180

ctgaagctga tttattattc agttggtgct ggtatcactg ataaaggaga agtcccgaat 240

ggctacaacg tctccagatc aaccacagag gatttcccgc tcaggctgga gttggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgaca gtccaagtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc cagtccaagt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1043

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1043

gccttgccag cccgctcagc agtccaagta cagtgcttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccgcatc ctgaagatag gacagagcat gacactgcag 120

tgtacccagg atatgaacca taactacatg tactggtatc gacaagaccc aggcatgggg 180

ctgaagctga tttattattc agttggtgct ggtatcactg ataaaggaga agtcccgaat 240

ggctacaacg tctccagatc aaccacagag gatttcccgc tcaggctgga gttggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgaca gtccaagtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg cagtccaagt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1044

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1044

gccttgccag cccgctcagc agtccaagga tcatccttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccgcatc ctgaagatag gacagagcat gacactgcag 120

tgtacccagg atatgaacca taactacatg tactggtatc gacaagaccc aggcatgggg 180

ctgaagctga tttattattc agttggtgct ggtatcactg ataaaggaga agtcccgaat 240

ggctacaacg tctccagatc aaccacagag gatttcccgc tcaggctgga gttggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgaca gtccaaggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg cagtccaagg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1045

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1045

gccttgccag cccgctcagc agtccaatat tggcgcttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccgcatc ctgaagatag gacagagcat gacactgcag 120

tgtacccagg atatgaacca taactacatg tactggtatc gacaagaccc aggcatgggg 180

ctgaagctga tttattattc agttggtgct ggtatcactg ataaaggaga agtcccgaat 240

ggctacaacg tctccagatc aaccacagag gatttcccgc tcaggctgga gttggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgaca gtccaatatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg cagtccaata ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1046

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1046

gccttgccag cccgctcagc agtccaaagg cttgacttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccgcatc ctgaagatag gacagagcat gacactgcag 120

tgtacccagg atatgaacca taactacatg tactggtatc gacaagaccc aggcatgggg 180

ctgaagctga tttattattc agttggtgct ggtatcactg ataaaggaga agtcccgaat 240

ggctacaacg tctccagatc aaccacagag gatttcccgc tcaggctgga gttggctgct 300

ccctcccaga catctgtgta cttctgtgcc agcagtgaca gtccaaaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg cagtccaaag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1047

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1047

gccttgccag cccgctcagt acgtacggac actctcttcc ctgcaggtcc aatgaatgct 60

ggtgtcactc agaccccaaa attccacgtc ctgaagacag gacagagcat gactctgctg 120

tgtgcccagg atatgaacca tgaatacatg tatcggtatc gacaagaccc aggcaagggg 180

ctgaggctga tttactactc agttgctgct gctctcactg acaaaggaga agttcccaat 240

ggctacaatg tctccagatc aaacacagag gatttccccc tcaagctgga gtcagctgct 300

ccctctcaga cttctgttta cttctgtgcc agcagtgata cgtacggaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag tacgtacgga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1048

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1048

gccttgccag cccgctcagt acgtacgttc ggaaccttcc ctgcaggtcc aatgaatgct 60

ggtgtcactc agaccccaaa attccacgtc ctgaagacag gacagagcat gactctgctg 120

tgtgcccagg atatgaacca tgaatacatg tatcggtatc gacaagaccc aggcaagggg 180

ctgaggctga tttactactc agttgctgct gctctcactg acaaaggaga agttcccaat 240

ggctacaatg tctccagatc aaacacagag gatttccccc tcaagctgga gtcagctgct 300

ccctctcaga cttctgttta cttctgtgcc agcagtgata cgtacgttcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat tacgtacgtt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1049

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1049

gccttgccag cccgctcagt acgtacgaag taacgcttcc ctgcaggtcc aatgaatgct 60

ggtgtcactc agaccccaaa attccacgtc ctgaagacag gacagagcat gactctgctg 120

tgtgcccagg atatgaacca tgaatacatg tatcggtatc gacaagaccc aggcaagggg 180

ctgaggctga tttactactc agttgctgct gctctcactg acaaaggaga agttcccaat 240

ggctacaatg tctccagatc aaacacagag gatttccccc tcaagctgga gtcagctgct 300

ccctctcaga cttctgttta cttctgtgcc agcagtgata cgtacgaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag tacgtacgaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1050

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1050

gccttgccag cccgctcagt acgtacggtc tcctacttcc ctgcaggtcc aatgaatgct 60

ggtgtcactc agaccccaaa attccacgtc ctgaagacag gacagagcat gactctgctg 120

tgtgcccagg atatgaacca tgaatacatg tatcggtatc gacaagaccc aggcaagggg 180

ctgaggctga tttactactc agttgctgct gctctcactg acaaaggaga agttcccaat 240

ggctacaatg tctccagatc aaacacagag gatttccccc tcaagctgga gtcagctgct 300

ccctctcaga cttctgttta cttctgtgcc agcagtgata cgtacggtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag tacgtacggt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1051

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1051

gccttgccag cccgctcagt acgtacgaga gtgtccttcc ctgcaggtcc aatgaatgct 60

ggtgtcactc agaccccaaa attccacgtc ctgaagacag gacagagcat gactctgctg 120

tgtgcccagg atatgaacca tgaatacatg tatcggtatc gacaagaccc aggcaagggg 180

ctgaggctga tttactactc agttgctgct gctctcactg acaaaggaga agttcccaat 240

ggctacaatg tctccagatc aaacacagag gatttccccc tcaagctgga gtcagctgct 300

ccctctcaga cttctgttta cttctgtgcc agcagtgata cgtacgagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct tacgtacgag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1052

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1052

gccttgccag cccgctcagt acgtacggtt ccgaacttcc ctgcaggtcc aatgaatgct 60

ggtgtcactc agaccccaaa attccacgtc ctgaagacag gacagagcat gactctgctg 120

tgtgcccagg atatgaacca tgaatacatg tatcggtatc gacaagaccc aggcaagggg 180

ctgaggctga tttactactc agttgctgct gctctcactg acaaaggaga agttcccaat 240

ggctacaatg tctccagatc aaacacagag gatttccccc tcaagctgga gtcagctgct 300

ccctctcaga cttctgttta cttctgtgcc agcagtgata cgtacggttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat tacgtacggt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1053

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1053

gccttgccag cccgctcagt acgtacgcgt tacttcttcc ctgcaggtcc aatgaatgct 60

ggtgtcactc agaccccaaa attccacgtc ctgaagacag gacagagcat gactctgctg 120

tgtgcccagg atatgaacca tgaatacatg tatcggtatc gacaagaccc aggcaagggg 180

ctgaggctga tttactactc agttgctgct gctctcactg acaaaggaga agttcccaat 240

ggctacaatg tctccagatc aaacacagag gatttccccc tcaagctgga gtcagctgct 300

ccctctcaga cttctgttta cttctgtgcc agcagtgata cgtacgcgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc tacgtacgcg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1054

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1054

gccttgccag cccgctcagt acgtacgtag gagaccttcc ctgcaggtcc aatgaatgct 60

ggtgtcactc agaccccaaa attccacgtc ctgaagacag gacagagcat gactctgctg 120

tgtgcccagg atatgaacca tgaatacatg tatcggtatc gacaagaccc aggcaagggg 180

ctgaggctga tttactactc agttgctgct gctctcactg acaaaggaga agttcccaat 240

ggctacaatg tctccagatc aaacacagag gatttccccc tcaagctgga gtcagctgct 300

ccctctcaga cttctgttta cttctgtgcc agcagtgata cgtacgtagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg tacgtacgta ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1055

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1055

gccttgccag cccgctcagt acgtacggtg tctaccttcc ctgcaggtcc aatgaatgct 60

ggtgtcactc agaccccaaa attccacgtc ctgaagacag gacagagcat gactctgctg 120

tgtgcccagg atatgaacca tgaatacatg tatcggtatc gacaagaccc aggcaagggg 180

ctgaggctga tttactactc agttgctgct gctctcactg acaaaggaga agttcccaat 240

ggctacaatg tctccagatc aaacacagag gatttccccc tcaagctgga gtcagctgct 300

ccctctcaga cttctgttta cttctgtgcc agcagtgata cgtacggtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc tacgtacggt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1056

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1056

gccttgccag cccgctcagt acgtacggta cagtgcttcc ctgcaggtcc aatgaatgct 60

ggtgtcactc agaccccaaa attccacgtc ctgaagacag gacagagcat gactctgctg 120

tgtgcccagg atatgaacca tgaatacatg tatcggtatc gacaagaccc aggcaagggg 180

ctgaggctga tttactactc agttgctgct gctctcactg acaaaggaga agttcccaat 240

ggctacaatg tctccagatc aaacacagag gatttccccc tcaagctgga gtcagctgct 300

ccctctcaga cttctgttta cttctgtgcc agcagtgata cgtacggtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg tacgtacggt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1057

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1057

gccttgccag cccgctcagt acgtacggga tcatccttcc ctgcaggtcc aatgaatgct 60

ggtgtcactc agaccccaaa attccacgtc ctgaagacag gacagagcat gactctgctg 120

tgtgcccagg atatgaacca tgaatacatg tatcggtatc gacaagaccc aggcaagggg 180

ctgaggctga tttactactc agttgctgct gctctcactg acaaaggaga agttcccaat 240

ggctacaatg tctccagatc aaacacagag gatttccccc tcaagctgga gtcagctgct 300

ccctctcaga cttctgttta cttctgtgcc agcagtgata cgtacgggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg tacgtacggg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1058

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1058

gccttgccag cccgctcagt acgtacgtat tggcgcttcc ctgcaggtcc aatgaatgct 60

ggtgtcactc agaccccaaa attccacgtc ctgaagacag gacagagcat gactctgctg 120

tgtgcccagg atatgaacca tgaatacatg tatcggtatc gacaagaccc aggcaagggg 180

ctgaggctga tttactactc agttgctgct gctctcactg acaaaggaga agttcccaat 240

ggctacaatg tctccagatc aaacacagag gatttccccc tcaagctgga gtcagctgct 300

ccctctcaga cttctgttta cttctgtgcc agcagtgata cgtacgtatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg tacgtacgta ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1059

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1059

gccttgccag cccgctcagt acgtacgagg cttgacttcc ctgcaggtcc aatgaatgct 60

ggtgtcactc agaccccaaa attccacgtc ctgaagacag gacagagcat gactctgctg 120

tgtgcccagg atatgaacca tgaatacatg tatcggtatc gacaagaccc aggcaagggg 180

ctgaggctga tttactactc agttgctgct gctctcactg acaaaggaga agttcccaat 240

ggctacaatg tctccagatc aaacacagag gatttccccc tcaagctgga gtcagctgct 300

ccctctcaga cttctgttta cttctgtgcc agcagtgata cgtacgaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg tacgtacgag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1060

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1060

gccttgccag cccgctcaga gtaccgagac actctgttct tctctgcagg tcccgtgaat 60

gctggtgtca ctcagacccc aaaattccac atcctgaaga caggacagag catgacactg 120

cagtgtgccc aggatatgaa ccatggatac atgtcctggt atcgacaaga cccaggcatg 180

gggctgagac tgatttacta ctcagctgct gctggtacta ctgacaaaga agtccccaat 240

ggctacaatg tctctagatt aaacacagag gatttcccac tcaggctggt gtcggctgct 300

ccctcccaga catctgtgta cttgtgtgcc agcagtgaag taccgagaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag agtaccgaga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1061

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1061

gccttgccag cccgctcaga gtaccgattc ggaacgttct tctctgcagg tcccgtgaat 60

gctggtgtca ctcagacccc aaaattccac atcctgaaga caggacagag catgacactg 120

cagtgtgccc aggatatgaa ccatggatac atgtcctggt atcgacaaga cccaggcatg 180

gggctgagac tgatttacta ctcagctgct gctggtacta ctgacaaaga agtccccaat 240

ggctacaatg tctctagatt aaacacagag gatttcccac tcaggctggt gtcggctgct 300

ccctcccaga catctgtgta cttgtgtgcc agcagtgaag taccgattcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat agtaccgatt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1062

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1062

gccttgccag cccgctcaga gtaccgaaag taacggttct tctctgcagg tcccgtgaat 60

gctggtgtca ctcagacccc aaaattccac atcctgaaga caggacagag catgacactg 120

cagtgtgccc aggatatgaa ccatggatac atgtcctggt atcgacaaga cccaggcatg 180

gggctgagac tgatttacta ctcagctgct gctggtacta ctgacaaaga agtccccaat 240

ggctacaatg tctctagatt aaacacagag gatttcccac tcaggctggt gtcggctgct 300

ccctcccaga catctgtgta cttgtgtgcc agcagtgaag taccgaaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag agtaccgaaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1063

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1063

gccttgccag cccgctcaga gtaccgagtc tcctagttct tctctgcagg tcccgtgaat 60

gctggtgtca ctcagacccc aaaattccac atcctgaaga caggacagag catgacactg 120

cagtgtgccc aggatatgaa ccatggatac atgtcctggt atcgacaaga cccaggcatg 180

gggctgagac tgatttacta ctcagctgct gctggtacta ctgacaaaga agtccccaat 240

ggctacaatg tctctagatt aaacacagag gatttcccac tcaggctggt gtcggctgct 300

ccctcccaga catctgtgta cttgtgtgcc agcagtgaag taccgagtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag agtaccgagt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1064

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1064

gccttgccag cccgctcaga gtaccgaaga gtgtcgttct tctctgcagg tcccgtgaat 60

gctggtgtca ctcagacccc aaaattccac atcctgaaga caggacagag catgacactg 120

cagtgtgccc aggatatgaa ccatggatac atgtcctggt atcgacaaga cccaggcatg 180

gggctgagac tgatttacta ctcagctgct gctggtacta ctgacaaaga agtccccaat 240

ggctacaatg tctctagatt aaacacagag gatttcccac tcaggctggt gtcggctgct 300

ccctcccaga catctgtgta cttgtgtgcc agcagtgaag taccgaagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct agtaccgaag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1065

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1065

gccttgccag cccgctcaga gtaccgagtt ccgaagttct tctctgcagg tcccgtgaat 60

gctggtgtca ctcagacccc aaaattccac atcctgaaga caggacagag catgacactg 120

cagtgtgccc aggatatgaa ccatggatac atgtcctggt atcgacaaga cccaggcatg 180

gggctgagac tgatttacta ctcagctgct gctggtacta ctgacaaaga agtccccaat 240

ggctacaatg tctctagatt aaacacagag gatttcccac tcaggctggt gtcggctgct 300

ccctcccaga catctgtgta cttgtgtgcc agcagtgaag taccgagttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat agtaccgagt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1066

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1066

gccttgccag cccgctcaga gtaccgacgt tacttgttct tctctgcagg tcccgtgaat 60

gctggtgtca ctcagacccc aaaattccac atcctgaaga caggacagag catgacactg 120

cagtgtgccc aggatatgaa ccatggatac atgtcctggt atcgacaaga cccaggcatg 180

gggctgagac tgatttacta ctcagctgct gctggtacta ctgacaaaga agtccccaat 240

ggctacaatg tctctagatt aaacacagag gatttcccac tcaggctggt gtcggctgct 300

ccctcccaga catctgtgta cttgtgtgcc agcagtgaag taccgacgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc agtaccgacg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1067

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1067

gccttgccag cccgctcaga gtaccgatag gagacgttct tctctgcagg tcccgtgaat 60

gctggtgtca ctcagacccc aaaattccac atcctgaaga caggacagag catgacactg 120

cagtgtgccc aggatatgaa ccatggatac atgtcctggt atcgacaaga cccaggcatg 180

gggctgagac tgatttacta ctcagctgct gctggtacta ctgacaaaga agtccccaat 240

ggctacaatg tctctagatt aaacacagag gatttcccac tcaggctggt gtcggctgct 300

ccctcccaga catctgtgta cttgtgtgcc agcagtgaag taccgatagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg agtaccgata ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1068

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1068

gccttgccag cccgctcaga gtaccgagtg tctacgttct tctctgcagg tcccgtgaat 60

gctggtgtca ctcagacccc aaaattccac atcctgaaga caggacagag catgacactg 120

cagtgtgccc aggatatgaa ccatggatac atgtcctggt atcgacaaga cccaggcatg 180

gggctgagac tgatttacta ctcagctgct gctggtacta ctgacaaaga agtccccaat 240

ggctacaatg tctctagatt aaacacagag gatttcccac tcaggctggt gtcggctgct 300

ccctcccaga catctgtgta cttgtgtgcc agcagtgaag taccgagtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc agtaccgagt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1069

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1069

gccttgccag cccgctcaga gtaccgagta cagtggttct tctctgcagg tcccgtgaat 60

gctggtgtca ctcagacccc aaaattccac atcctgaaga caggacagag catgacactg 120

cagtgtgccc aggatatgaa ccatggatac atgtcctggt atcgacaaga cccaggcatg 180

gggctgagac tgatttacta ctcagctgct gctggtacta ctgacaaaga agtccccaat 240

ggctacaatg tctctagatt aaacacagag gatttcccac tcaggctggt gtcggctgct 300

ccctcccaga catctgtgta cttgtgtgcc agcagtgaag taccgagtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg agtaccgagt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1070

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1070

gccttgccag cccgctcaga gtaccgagga tcatcgttct tctctgcagg tcccgtgaat 60

gctggtgtca ctcagacccc aaaattccac atcctgaaga caggacagag catgacactg 120

cagtgtgccc aggatatgaa ccatggatac atgtcctggt atcgacaaga cccaggcatg 180

gggctgagac tgatttacta ctcagctgct gctggtacta ctgacaaaga agtccccaat 240

ggctacaatg tctctagatt aaacacagag gatttcccac tcaggctggt gtcggctgct 300

ccctcccaga catctgtgta cttgtgtgcc agcagtgaag taccgaggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg agtaccgagg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1071

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1071

gccttgccag cccgctcaga gtaccgatat tggcggttct tctctgcagg tcccgtgaat 60

gctggtgtca ctcagacccc aaaattccac atcctgaaga caggacagag catgacactg 120

cagtgtgccc aggatatgaa ccatggatac atgtcctggt atcgacaaga cccaggcatg 180

gggctgagac tgatttacta ctcagctgct gctggtacta ctgacaaaga agtccccaat 240

ggctacaatg tctctagatt aaacacagag gatttcccac tcaggctggt gtcggctgct 300

ccctcccaga catctgtgta cttgtgtgcc agcagtgaag taccgatatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg agtaccgata ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1072

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1072

gccttgccag cccgctcaga gtaccgaagg cttgagttct tctctgcagg tcccgtgaat 60

gctggtgtca ctcagacccc aaaattccac atcctgaaga caggacagag catgacactg 120

cagtgtgccc aggatatgaa ccatggatac atgtcctggt atcgacaaga cccaggcatg 180

gggctgagac tgatttacta ctcagctgct gctggtacta ctgacaaaga agtccccaat 240

ggctacaatg tctctagatt aaacacagag gatttcccac tcaggctggt gtcggctgct 300

ccctcccaga catctgtgta cttgtgtgcc agcagtgaag taccgaaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg agtaccgaag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1073

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1073

gccttgccag cccgctcaga tccatgggac actctcttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccacatc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca tggatacttg tcctggtatc gacaagaccc aggcatgggg 180

ctgaggcgca ttcattactc agttgctgct ggtatcactg acaaaggaga agtccccgat 240

ggctacaatg tatccagatc aaacacagag gatttcccgc tcaggctgga gtcagctgct 300

ccctcccaga catctgtata cttctgtgcc agcagtgaat ccatgggaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag atccatggga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1074

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1074

gccttgccag cccgctcaga tccatggttc ggaaccttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccacatc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca tggatacttg tcctggtatc gacaagaccc aggcatgggg 180

ctgaggcgca ttcattactc agttgctgct ggtatcactg acaaaggaga agtccccgat 240

ggctacaatg tatccagatc aaacacagag gatttcccgc tcaggctgga gtcagctgct 300

ccctcccaga catctgtata cttctgtgcc agcagtgaat ccatggttcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat atccatggtt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1075

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1075

gccttgccag cccgctcaga tccatggaag taacgcttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccacatc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca tggatacttg tcctggtatc gacaagaccc aggcatgggg 180

ctgaggcgca ttcattactc agttgctgct ggtatcactg acaaaggaga agtccccgat 240

ggctacaatg tatccagatc aaacacagag gatttcccgc tcaggctgga gtcagctgct 300

ccctcccaga catctgtata cttctgtgcc agcagtgaat ccatggaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag atccatggaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1076

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1076

gccttgccag cccgctcaga tccatgggtc tcctacttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccacatc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca tggatacttg tcctggtatc gacaagaccc aggcatgggg 180

ctgaggcgca ttcattactc agttgctgct ggtatcactg acaaaggaga agtccccgat 240

ggctacaatg tatccagatc aaacacagag gatttcccgc tcaggctgga gtcagctgct 300

ccctcccaga catctgtata cttctgtgcc agcagtgaat ccatgggtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag atccatgggt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1077

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1077

gccttgccag cccgctcaga tccatggaga gtgtccttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccacatc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca tggatacttg tcctggtatc gacaagaccc aggcatgggg 180

ctgaggcgca ttcattactc agttgctgct ggtatcactg acaaaggaga agtccccgat 240

ggctacaatg tatccagatc aaacacagag gatttcccgc tcaggctgga gtcagctgct 300

ccctcccaga catctgtata cttctgtgcc agcagtgaat ccatggagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct atccatggag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1078

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1078

gccttgccag cccgctcaga tccatgggtt ccgaacttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccacatc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca tggatacttg tcctggtatc gacaagaccc aggcatgggg 180

ctgaggcgca ttcattactc agttgctgct ggtatcactg acaaaggaga agtccccgat 240

ggctacaatg tatccagatc aaacacagag gatttcccgc tcaggctgga gtcagctgct 300

ccctcccaga catctgtata cttctgtgcc agcagtgaat ccatgggttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat atccatgggt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1079

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1079

gccttgccag cccgctcaga tccatggcgt tacttcttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccacatc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca tggatacttg tcctggtatc gacaagaccc aggcatgggg 180

ctgaggcgca ttcattactc agttgctgct ggtatcactg acaaaggaga agtccccgat 240

ggctacaatg tatccagatc aaacacagag gatttcccgc tcaggctgga gtcagctgct 300

ccctcccaga catctgtata cttctgtgcc agcagtgaat ccatggcgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc atccatggcg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1080

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1080

gccttgccag cccgctcaga tccatggtag gagaccttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccacatc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca tggatacttg tcctggtatc gacaagaccc aggcatgggg 180

ctgaggcgca ttcattactc agttgctgct ggtatcactg acaaaggaga agtccccgat 240

ggctacaatg tatccagatc aaacacagag gatttcccgc tcaggctgga gtcagctgct 300

ccctcccaga catctgtata cttctgtgcc agcagtgaat ccatggtagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg atccatggta ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1081

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1081

gccttgccag cccgctcaga tccatgggtg tctaccttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccacatc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca tggatacttg tcctggtatc gacaagaccc aggcatgggg 180

ctgaggcgca ttcattactc agttgctgct ggtatcactg acaaaggaga agtccccgat 240

ggctacaatg tatccagatc aaacacagag gatttcccgc tcaggctgga gtcagctgct 300

ccctcccaga catctgtata cttctgtgcc agcagtgaat ccatgggtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc atccatgggt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1082

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1082

gccttgccag cccgctcaga tccatgggta cagtgcttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccacatc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca tggatacttg tcctggtatc gacaagaccc aggcatgggg 180

ctgaggcgca ttcattactc agttgctgct ggtatcactg acaaaggaga agtccccgat 240

ggctacaatg tatccagatc aaacacagag gatttcccgc tcaggctgga gtcagctgct 300

ccctcccaga catctgtata cttctgtgcc agcagtgaat ccatgggtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg atccatgggt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1083

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1083

gccttgccag cccgctcaga tccatgggga tcatccttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccacatc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca tggatacttg tcctggtatc gacaagaccc aggcatgggg 180

ctgaggcgca ttcattactc agttgctgct ggtatcactg acaaaggaga agtccccgat 240

ggctacaatg tatccagatc aaacacagag gatttcccgc tcaggctgga gtcagctgct 300

ccctcccaga catctgtata cttctgtgcc agcagtgaat ccatggggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg atccatgggg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1084

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1084

gccttgccag cccgctcaga tccatggtat tggcgcttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccacatc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca tggatacttg tcctggtatc gacaagaccc aggcatgggg 180

ctgaggcgca ttcattactc agttgctgct ggtatcactg acaaaggaga agtccccgat 240

ggctacaatg tatccagatc aaacacagag gatttcccgc tcaggctgga gtcagctgct 300

ccctcccaga catctgtata cttctgtgcc agcagtgaat ccatggtatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg atccatggta ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1085

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1085

gccttgccag cccgctcaga tccatggagg cttgacttct ctgcaggtcc agtgaatgct 60

ggtgtcactc agaccccaaa attccacatc ctgaagacag gacagagcat gacactgcag 120

tgtgcccagg atatgaacca tggatacttg tcctggtatc gacaagaccc aggcatgggg 180

ctgaggcgca ttcattactc agttgctgct ggtatcactg acaaaggaga agtccccgat 240

ggctacaatg tatccagatc aaacacagag gatttcccgc tcaggctgga gtcagctgct 300

ccctcccaga catctgtata cttctgtgcc agcagtgaat ccatggaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg atccatggag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1086

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1086

gccttgccag cccgctcagg tagcagtgac actctctcca cagatcacac aggtgctgga 60

gtctcccagt ccctgagaca caaggtagca aagaagggaa aggatgtagc tctcagatat 120

gatccaattt caggtcataa tgccctttat tggtaccgac agagcctggg gcagggcctg 180

gagtttccaa tttacttcca aggcaaggat gcagcagaca aatcggggct tccccgtgat 240

cggttctctg cacagaggtc tgagggatcc atctccactc tgaagttcca gcgcacacag 300

cagggggact tggctgtgta tctctgtgcc agcagtgagt agcagtgaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag gtagcagtga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1087

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1087

gccttgccag cccgctcagg tagcagtttc ggaacctcca cagatcacac aggtgctgga 60

gtctcccagt ccctgagaca caaggtagca aagaagggaa aggatgtagc tctcagatat 120

gatccaattt caggtcataa tgccctttat tggtaccgac agagcctggg gcagggcctg 180

gagtttccaa tttacttcca aggcaaggat gcagcagaca aatcggggct tccccgtgat 240

cggttctctg cacagaggtc tgagggatcc atctccactc tgaagttcca gcgcacacag 300

cagggggact tggctgtgta tctctgtgcc agcagtgagt agcagtttcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat gtagcagttt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1088

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1088

gccttgccag cccgctcagg tagcagtaag taacgctcca cagatcacac aggtgctgga 60

gtctcccagt ccctgagaca caaggtagca aagaagggaa aggatgtagc tctcagatat 120

gatccaattt caggtcataa tgccctttat tggtaccgac agagcctggg gcagggcctg 180

gagtttccaa tttacttcca aggcaaggat gcagcagaca aatcggggct tccccgtgat 240

cggttctctg cacagaggtc tgagggatcc atctccactc tgaagttcca gcgcacacag 300

cagggggact tggctgtgta tctctgtgcc agcagtgagt agcagtaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag gtagcagtaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1089

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1089

gccttgccag cccgctcagg tagcagtgtc tcctactcca cagatcacac aggtgctgga 60

gtctcccagt ccctgagaca caaggtagca aagaagggaa aggatgtagc tctcagatat 120

gatccaattt caggtcataa tgccctttat tggtaccgac agagcctggg gcagggcctg 180

gagtttccaa tttacttcca aggcaaggat gcagcagaca aatcggggct tccccgtgat 240

cggttctctg cacagaggtc tgagggatcc atctccactc tgaagttcca gcgcacacag 300

cagggggact tggctgtgta tctctgtgcc agcagtgagt agcagtgtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag gtagcagtgt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1090

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1090

gccttgccag cccgctcagg tagcagtaga gtgtcctcca cagatcacac aggtgctgga 60

gtctcccagt ccctgagaca caaggtagca aagaagggaa aggatgtagc tctcagatat 120

gatccaattt caggtcataa tgccctttat tggtaccgac agagcctggg gcagggcctg 180

gagtttccaa tttacttcca aggcaaggat gcagcagaca aatcggggct tccccgtgat 240

cggttctctg cacagaggtc tgagggatcc atctccactc tgaagttcca gcgcacacag 300

cagggggact tggctgtgta tctctgtgcc agcagtgagt agcagtagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct gtagcagtag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1091

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1091

gccttgccag cccgctcagg tagcagtgtt ccgaactcca cagatcacac aggtgctgga 60

gtctcccagt ccctgagaca caaggtagca aagaagggaa aggatgtagc tctcagatat 120

gatccaattt caggtcataa tgccctttat tggtaccgac agagcctggg gcagggcctg 180

gagtttccaa tttacttcca aggcaaggat gcagcagaca aatcggggct tccccgtgat 240

cggttctctg cacagaggtc tgagggatcc atctccactc tgaagttcca gcgcacacag 300

cagggggact tggctgtgta tctctgtgcc agcagtgagt agcagtgttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat gtagcagtgt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1092

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1092

gccttgccag cccgctcagg tagcagtcgt tacttctcca cagatcacac aggtgctgga 60

gtctcccagt ccctgagaca caaggtagca aagaagggaa aggatgtagc tctcagatat 120

gatccaattt caggtcataa tgccctttat tggtaccgac agagcctggg gcagggcctg 180

gagtttccaa tttacttcca aggcaaggat gcagcagaca aatcggggct tccccgtgat 240

cggttctctg cacagaggtc tgagggatcc atctccactc tgaagttcca gcgcacacag 300

cagggggact tggctgtgta tctctgtgcc agcagtgagt agcagtcgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc gtagcagtcg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1093

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1093

gccttgccag cccgctcagg tagcagttag gagacctcca cagatcacac aggtgctgga 60

gtctcccagt ccctgagaca caaggtagca aagaagggaa aggatgtagc tctcagatat 120

gatccaattt caggtcataa tgccctttat tggtaccgac agagcctggg gcagggcctg 180

gagtttccaa tttacttcca aggcaaggat gcagcagaca aatcggggct tccccgtgat 240

cggttctctg cacagaggtc tgagggatcc atctccactc tgaagttcca gcgcacacag 300

cagggggact tggctgtgta tctctgtgcc agcagtgagt agcagttagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg gtagcagtta ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1094

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1094

gccttgccag cccgctcagg tagcagtgtg tctacctcca cagatcacac aggtgctgga 60

gtctcccagt ccctgagaca caaggtagca aagaagggaa aggatgtagc tctcagatat 120

gatccaattt caggtcataa tgccctttat tggtaccgac agagcctggg gcagggcctg 180

gagtttccaa tttacttcca aggcaaggat gcagcagaca aatcggggct tccccgtgat 240

cggttctctg cacagaggtc tgagggatcc atctccactc tgaagttcca gcgcacacag 300

cagggggact tggctgtgta tctctgtgcc agcagtgagt agcagtgtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc gtagcagtgt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1095

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1095

gccttgccag cccgctcagg tagcagtgta cagtgctcca cagatcacac aggtgctgga 60

gtctcccagt ccctgagaca caaggtagca aagaagggaa aggatgtagc tctcagatat 120

gatccaattt caggtcataa tgccctttat tggtaccgac agagcctggg gcagggcctg 180

gagtttccaa tttacttcca aggcaaggat gcagcagaca aatcggggct tccccgtgat 240

cggttctctg cacagaggtc tgagggatcc atctccactc tgaagttcca gcgcacacag 300

cagggggact tggctgtgta tctctgtgcc agcagtgagt agcagtgtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg gtagcagtgt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1096

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1096

gccttgccag cccgctcagg tagcagtgga tcatcctcca cagatcacac aggtgctgga 60

gtctcccagt ccctgagaca caaggtagca aagaagggaa aggatgtagc tctcagatat 120

gatccaattt caggtcataa tgccctttat tggtaccgac agagcctggg gcagggcctg 180

gagtttccaa tttacttcca aggcaaggat gcagcagaca aatcggggct tccccgtgat 240

cggttctctg cacagaggtc tgagggatcc atctccactc tgaagttcca gcgcacacag 300

cagggggact tggctgtgta tctctgtgcc agcagtgagt agcagtggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg gtagcagtgg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1097

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1097

gccttgccag cccgctcagg tagcagttat tggcgctcca cagatcacac aggtgctgga 60

gtctcccagt ccctgagaca caaggtagca aagaagggaa aggatgtagc tctcagatat 120

gatccaattt caggtcataa tgccctttat tggtaccgac agagcctggg gcagggcctg 180

gagtttccaa tttacttcca aggcaaggat gcagcagaca aatcggggct tccccgtgat 240

cggttctctg cacagaggtc tgagggatcc atctccactc tgaagttcca gcgcacacag 300

cagggggact tggctgtgta tctctgtgcc agcagtgagt agcagttatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg gtagcagtta ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1098

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1098

gccttgccag cccgctcagg tagcagtagg cttgactcca cagatcacac aggtgctgga 60

gtctcccagt ccctgagaca caaggtagca aagaagggaa aggatgtagc tctcagatat 120

gatccaattt caggtcataa tgccctttat tggtaccgac agagcctggg gcagggcctg 180

gagtttccaa tttacttcca aggcaaggat gcagcagaca aatcggggct tccccgtgat 240

cggttctctg cacagaggtc tgagggatcc atctccactc tgaagttcca gcgcacacag 300

cagggggact tggctgtgta tctctgtgcc agcagtgagt agcagtaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg gtagcagtag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1099

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1099

gccttgccag cccgctcaga tcttcgtgac actctctcca cagatcacac aggagctgga 60

gtctcccagt cccccagtaa caaggtcaca gagaagggaa aggatgtaga gctcaggtgt 120

gatccaattt caggtcatac tgccctttac tggtaccgac agagcctggg gcagggcctg 180

gagtttttaa tttacttcca aggcaacagt gcaccagaca aatcagggct gcccagtgat 240

cgcttctctg cagagaggac tgggggatcc gtctccactc tgacgatcca gcgcacacag 300

caggaggact cggccgtgta tctctgtgcc agcagtgaat cttcgtgaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag atcttcgtga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1100

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1100

gccttgccag cccgctcaga tcttcgtttc ggaacctcca cagatcacac aggagctgga 60

gtctcccagt cccccagtaa caaggtcaca gagaagggaa aggatgtaga gctcaggtgt 120

gatccaattt caggtcatac tgccctttac tggtaccgac agagcctggg gcagggcctg 180

gagtttttaa tttacttcca aggcaacagt gcaccagaca aatcagggct gcccagtgat 240

cgcttctctg cagagaggac tgggggatcc gtctccactc tgacgatcca gcgcacacag 300

caggaggact cggccgtgta tctctgtgcc agcagtgaat cttcgtttcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat atcttcgttt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1101

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1101

gccttgccag cccgctcaga tcttcgtaag taacgctcca cagatcacac aggagctgga 60

gtctcccagt cccccagtaa caaggtcaca gagaagggaa aggatgtaga gctcaggtgt 120

gatccaattt caggtcatac tgccctttac tggtaccgac agagcctggg gcagggcctg 180

gagtttttaa tttacttcca aggcaacagt gcaccagaca aatcagggct gcccagtgat 240

cgcttctctg cagagaggac tgggggatcc gtctccactc tgacgatcca gcgcacacag 300

caggaggact cggccgtgta tctctgtgcc agcagtgaat cttcgtaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag atcttcgtaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1102

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1102

gccttgccag cccgctcaga tcttcgtgtc tcctactcca cagatcacac aggagctgga 60

gtctcccagt cccccagtaa caaggtcaca gagaagggaa aggatgtaga gctcaggtgt 120

gatccaattt caggtcatac tgccctttac tggtaccgac agagcctggg gcagggcctg 180

gagtttttaa tttacttcca aggcaacagt gcaccagaca aatcagggct gcccagtgat 240

cgcttctctg cagagaggac tgggggatcc gtctccactc tgacgatcca gcgcacacag 300

caggaggact cggccgtgta tctctgtgcc agcagtgaat cttcgtgtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag atcttcgtgt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1103

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1103

gccttgccag cccgctcaga tcttcgtaga gtgtcctcca cagatcacac aggagctgga 60

gtctcccagt cccccagtaa caaggtcaca gagaagggaa aggatgtaga gctcaggtgt 120

gatccaattt caggtcatac tgccctttac tggtaccgac agagcctggg gcagggcctg 180

gagtttttaa tttacttcca aggcaacagt gcaccagaca aatcagggct gcccagtgat 240

cgcttctctg cagagaggac tgggggatcc gtctccactc tgacgatcca gcgcacacag 300

caggaggact cggccgtgta tctctgtgcc agcagtgaat cttcgtagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct atcttcgtag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1104

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1104

gccttgccag cccgctcaga tcttcgtgtt ccgaactcca cagatcacac aggagctgga 60

gtctcccagt cccccagtaa caaggtcaca gagaagggaa aggatgtaga gctcaggtgt 120

gatccaattt caggtcatac tgccctttac tggtaccgac agagcctggg gcagggcctg 180

gagtttttaa tttacttcca aggcaacagt gcaccagaca aatcagggct gcccagtgat 240

cgcttctctg cagagaggac tgggggatcc gtctccactc tgacgatcca gcgcacacag 300

caggaggact cggccgtgta tctctgtgcc agcagtgaat cttcgtgttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat atcttcgtgt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1105

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1105

gccttgccag cccgctcaga tcttcgtcgt tacttctcca cagatcacac aggagctgga 60

gtctcccagt cccccagtaa caaggtcaca gagaagggaa aggatgtaga gctcaggtgt 120

gatccaattt caggtcatac tgccctttac tggtaccgac agagcctggg gcagggcctg 180

gagtttttaa tttacttcca aggcaacagt gcaccagaca aatcagggct gcccagtgat 240

cgcttctctg cagagaggac tgggggatcc gtctccactc tgacgatcca gcgcacacag 300

caggaggact cggccgtgta tctctgtgcc agcagtgaat cttcgtcgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc atcttcgtcg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1106

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1106

gccttgccag cccgctcaga tcttcgttag gagacctcca cagatcacac aggagctgga 60

gtctcccagt cccccagtaa caaggtcaca gagaagggaa aggatgtaga gctcaggtgt 120

gatccaattt caggtcatac tgccctttac tggtaccgac agagcctggg gcagggcctg 180

gagtttttaa tttacttcca aggcaacagt gcaccagaca aatcagggct gcccagtgat 240

cgcttctctg cagagaggac tgggggatcc gtctccactc tgacgatcca gcgcacacag 300

caggaggact cggccgtgta tctctgtgcc agcagtgaat cttcgttagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg atcttcgtta ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1107

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1107

gccttgccag cccgctcaga tcttcgtgtg tctacctcca cagatcacac aggagctgga 60

gtctcccagt cccccagtaa caaggtcaca gagaagggaa aggatgtaga gctcaggtgt 120

gatccaattt caggtcatac tgccctttac tggtaccgac agagcctggg gcagggcctg 180

gagtttttaa tttacttcca aggcaacagt gcaccagaca aatcagggct gcccagtgat 240

cgcttctctg cagagaggac tgggggatcc gtctccactc tgacgatcca gcgcacacag 300

caggaggact cggccgtgta tctctgtgcc agcagtgaat cttcgtgtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc atcttcgtgt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1108

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1108

gccttgccag cccgctcaga tcttcgtgta cagtgctcca cagatcacac aggagctgga 60

gtctcccagt cccccagtaa caaggtcaca gagaagggaa aggatgtaga gctcaggtgt 120

gatccaattt caggtcatac tgccctttac tggtaccgac agagcctggg gcagggcctg 180

gagtttttaa tttacttcca aggcaacagt gcaccagaca aatcagggct gcccagtgat 240

cgcttctctg cagagaggac tgggggatcc gtctccactc tgacgatcca gcgcacacag 300

caggaggact cggccgtgta tctctgtgcc agcagtgaat cttcgtgtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg atcttcgtgt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1109

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1109

gccttgccag cccgctcaga tcttcgtgga tcatcctcca cagatcacac aggagctgga 60

gtctcccagt cccccagtaa caaggtcaca gagaagggaa aggatgtaga gctcaggtgt 120

gatccaattt caggtcatac tgccctttac tggtaccgac agagcctggg gcagggcctg 180

gagtttttaa tttacttcca aggcaacagt gcaccagaca aatcagggct gcccagtgat 240

cgcttctctg cagagaggac tgggggatcc gtctccactc tgacgatcca gcgcacacag 300

caggaggact cggccgtgta tctctgtgcc agcagtgaat cttcgtggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg atcttcgtgg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1110

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1110

gccttgccag cccgctcaga tcttcgttat tggcgctcca cagatcacac aggagctgga 60

gtctcccagt cccccagtaa caaggtcaca gagaagggaa aggatgtaga gctcaggtgt 120

gatccaattt caggtcatac tgccctttac tggtaccgac agagcctggg gcagggcctg 180

gagtttttaa tttacttcca aggcaacagt gcaccagaca aatcagggct gcccagtgat 240

cgcttctctg cagagaggac tgggggatcc gtctccactc tgacgatcca gcgcacacag 300

caggaggact cggccgtgta tctctgtgcc agcagtgaat cttcgttatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg atcttcgtta ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1111

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1111

gccttgccag cccgctcaga tcttcgtagg cttgactcca cagatcacac aggagctgga 60

gtctcccagt cccccagtaa caaggtcaca gagaagggaa aggatgtaga gctcaggtgt 120

gatccaattt caggtcatac tgccctttac tggtaccgac agagcctggg gcagggcctg 180

gagtttttaa tttacttcca aggcaacagt gcaccagaca aatcagggct gcccagtgat 240

cgcttctctg cagagaggac tgggggatcc gtctccactc tgacgatcca gcgcacacag 300

caggaggact cggccgtgta tctctgtgcc agcagtgaat cttcgtaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg atcttcgtag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1112

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1112

gccttgccag cccgctcagt ccacagtgac actctctccg cagatcacac aggtgctgga 60

gtctcccaga cccccagtaa caaggtcaca gagaagggaa aatatgtaga gctcaggtgt 120

gatccaattt caggtcatac tgccctttac tggtaccgac aaagcctggg gcagggccca 180

gagtttctaa tttacttcca aggcacgggt gcggcagatg actcagggct gcccaacgat 240

cggttctttg cagtcaggcc tgagggatcc gtctctactc tgaagatcca gcgcacagag 300

cggggggact cagccgtgta tctctgtgcc agcagtgatc cacagtgaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag tccacagtga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1113

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1113

gccttgccag cccgctcagt ccacagtttc ggaacctccg cagatcacac aggtgctgga 60

gtctcccaga cccccagtaa caaggtcaca gagaagggaa aatatgtaga gctcaggtgt 120

gatccaattt caggtcatac tgccctttac tggtaccgac aaagcctggg gcagggccca 180

gagtttctaa tttacttcca aggcacgggt gcggcagatg actcagggct gcccaacgat 240

cggttctttg cagtcaggcc tgagggatcc gtctctactc tgaagatcca gcgcacagag 300

cggggggact cagccgtgta tctctgtgcc agcagtgatc cacagtttcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat tccacagttt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1114

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1114

gccttgccag cccgctcagt ccacagtaag taacgctccg cagatcacac aggtgctgga 60

gtctcccaga cccccagtaa caaggtcaca gagaagggaa aatatgtaga gctcaggtgt 120

gatccaattt caggtcatac tgccctttac tggtaccgac aaagcctggg gcagggccca 180

gagtttctaa tttacttcca aggcacgggt gcggcagatg actcagggct gcccaacgat 240

cggttctttg cagtcaggcc tgagggatcc gtctctactc tgaagatcca gcgcacagag 300

cggggggact cagccgtgta tctctgtgcc agcagtgatc cacagtaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag tccacagtaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1115

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1115

gccttgccag cccgctcagt ccacagtgtc tcctactccg cagatcacac aggtgctgga 60

gtctcccaga cccccagtaa caaggtcaca gagaagggaa aatatgtaga gctcaggtgt 120

gatccaattt caggtcatac tgccctttac tggtaccgac aaagcctggg gcagggccca 180

gagtttctaa tttacttcca aggcacgggt gcggcagatg actcagggct gcccaacgat 240

cggttctttg cagtcaggcc tgagggatcc gtctctactc tgaagatcca gcgcacagag 300

cggggggact cagccgtgta tctctgtgcc agcagtgatc cacagtgtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag tccacagtgt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1116

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1116

gccttgccag cccgctcagt ccacagtaga gtgtcctccg cagatcacac aggtgctgga 60

gtctcccaga cccccagtaa caaggtcaca gagaagggaa aatatgtaga gctcaggtgt 120

gatccaattt caggtcatac tgccctttac tggtaccgac aaagcctggg gcagggccca 180

gagtttctaa tttacttcca aggcacgggt gcggcagatg actcagggct gcccaacgat 240

cggttctttg cagtcaggcc tgagggatcc gtctctactc tgaagatcca gcgcacagag 300

cggggggact cagccgtgta tctctgtgcc agcagtgatc cacagtagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct tccacagtag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1117

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1117

gccttgccag cccgctcagt ccacagtgtt ccgaactccg cagatcacac aggtgctgga 60

gtctcccaga cccccagtaa caaggtcaca gagaagggaa aatatgtaga gctcaggtgt 120

gatccaattt caggtcatac tgccctttac tggtaccgac aaagcctggg gcagggccca 180

gagtttctaa tttacttcca aggcacgggt gcggcagatg actcagggct gcccaacgat 240

cggttctttg cagtcaggcc tgagggatcc gtctctactc tgaagatcca gcgcacagag 300

cggggggact cagccgtgta tctctgtgcc agcagtgatc cacagtgttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat tccacagtgt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1118

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1118

gccttgccag cccgctcagt ccacagtcgt tacttctccg cagatcacac aggtgctgga 60

gtctcccaga cccccagtaa caaggtcaca gagaagggaa aatatgtaga gctcaggtgt 120

gatccaattt caggtcatac tgccctttac tggtaccgac aaagcctggg gcagggccca 180

gagtttctaa tttacttcca aggcacgggt gcggcagatg actcagggct gcccaacgat 240

cggttctttg cagtcaggcc tgagggatcc gtctctactc tgaagatcca gcgcacagag 300

cggggggact cagccgtgta tctctgtgcc agcagtgatc cacagtcgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc tccacagtcg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1119

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1119

gccttgccag cccgctcagt ccacagttag gagacctccg cagatcacac aggtgctgga 60

gtctcccaga cccccagtaa caaggtcaca gagaagggaa aatatgtaga gctcaggtgt 120

gatccaattt caggtcatac tgccctttac tggtaccgac aaagcctggg gcagggccca 180

gagtttctaa tttacttcca aggcacgggt gcggcagatg actcagggct gcccaacgat 240

cggttctttg cagtcaggcc tgagggatcc gtctctactc tgaagatcca gcgcacagag 300

cggggggact cagccgtgta tctctgtgcc agcagtgatc cacagttagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg tccacagtta ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1120

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1120

gccttgccag cccgctcagt ccacagtgtg tctacctccg cagatcacac aggtgctgga 60

gtctcccaga cccccagtaa caaggtcaca gagaagggaa aatatgtaga gctcaggtgt 120

gatccaattt caggtcatac tgccctttac tggtaccgac aaagcctggg gcagggccca 180

gagtttctaa tttacttcca aggcacgggt gcggcagatg actcagggct gcccaacgat 240

cggttctttg cagtcaggcc tgagggatcc gtctctactc tgaagatcca gcgcacagag 300

cggggggact cagccgtgta tctctgtgcc agcagtgatc cacagtgtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc tccacagtgt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1121

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1121

gccttgccag cccgctcagt ccacagtgta cagtgctccg cagatcacac aggtgctgga 60

gtctcccaga cccccagtaa caaggtcaca gagaagggaa aatatgtaga gctcaggtgt 120

gatccaattt caggtcatac tgccctttac tggtaccgac aaagcctggg gcagggccca 180

gagtttctaa tttacttcca aggcacgggt gcggcagatg actcagggct gcccaacgat 240

cggttctttg cagtcaggcc tgagggatcc gtctctactc tgaagatcca gcgcacagag 300

cggggggact cagccgtgta tctctgtgcc agcagtgatc cacagtgtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg tccacagtgt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1122

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1122

gccttgccag cccgctcagt ccacagtgga tcatcctccg cagatcacac aggtgctgga 60

gtctcccaga cccccagtaa caaggtcaca gagaagggaa aatatgtaga gctcaggtgt 120

gatccaattt caggtcatac tgccctttac tggtaccgac aaagcctggg gcagggccca 180

gagtttctaa tttacttcca aggcacgggt gcggcagatg actcagggct gcccaacgat 240

cggttctttg cagtcaggcc tgagggatcc gtctctactc tgaagatcca gcgcacagag 300

cggggggact cagccgtgta tctctgtgcc agcagtgatc cacagtggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg tccacagtgg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1123

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1123

gccttgccag cccgctcagt ccacagttat tggcgctccg cagatcacac aggtgctgga 60

gtctcccaga cccccagtaa caaggtcaca gagaagggaa aatatgtaga gctcaggtgt 120

gatccaattt caggtcatac tgccctttac tggtaccgac aaagcctggg gcagggccca 180

gagtttctaa tttacttcca aggcacgggt gcggcagatg actcagggct gcccaacgat 240

cggttctttg cagtcaggcc tgagggatcc gtctctactc tgaagatcca gcgcacagag 300

cggggggact cagccgtgta tctctgtgcc agcagtgatc cacagttatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg tccacagtta ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1124

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1124

gccttgccag cccgctcagt ccacagtagg cttgactccg cagatcacac aggtgctgga 60

gtctcccaga cccccagtaa caaggtcaca gagaagggaa aatatgtaga gctcaggtgt 120

gatccaattt caggtcatac tgccctttac tggtaccgac aaagcctggg gcagggccca 180

gagtttctaa tttacttcca aggcacgggt gcggcagatg actcagggct gcccaacgat 240

cggttctttg cagtcaggcc tgagggatcc gtctctactc tgaagatcca gcgcacagag 300

cggggggact cagccgtgta tctctgtgcc agcagtgatc cacagtaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg tccacagtag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1125

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1125

gccttgccag cccgctcaga tgacaccgac actctttcca cagatcacac aggtgctgga 60

gtctcccagt ccccaaggta caaagtcgca aagaggggac gggatgtagc tctcaggtgt 120

gattcaattt cgggtcatgt aaccctttat tggtaccgac agaccctggg gcagggctca 180

gaggttctga cttactccca gagtgatgct caacgagaca aatcagggcg gcccagtggt 240

cggttctctg cagagaggcc tgagagatcc gtctccactc tgaagatcca gcgcacagag 300

cagggggact cagctgtgta tctctgtgcc agcagtgaat gacaccgaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag atgacaccga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1126

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1126

gccttgccag cccgctcaga tgacaccttc ggaacttcca cagatcacac aggtgctgga 60

gtctcccagt ccccaaggta caaagtcgca aagaggggac gggatgtagc tctcaggtgt 120

gattcaattt cgggtcatgt aaccctttat tggtaccgac agaccctggg gcagggctca 180

gaggttctga cttactccca gagtgatgct caacgagaca aatcagggcg gcccagtggt 240

cggttctctg cagagaggcc tgagagatcc gtctccactc tgaagatcca gcgcacagag 300

cagggggact cagctgtgta tctctgtgcc agcagtgaat gacaccttcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat atgacacctt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1127

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1127

gccttgccag cccgctcaga tgacaccaag taacgttcca cagatcacac aggtgctgga 60

gtctcccagt ccccaaggta caaagtcgca aagaggggac gggatgtagc tctcaggtgt 120

gattcaattt cgggtcatgt aaccctttat tggtaccgac agaccctggg gcagggctca 180

gaggttctga cttactccca gagtgatgct caacgagaca aatcagggcg gcccagtggt 240

cggttctctg cagagaggcc tgagagatcc gtctccactc tgaagatcca gcgcacagag 300

cagggggact cagctgtgta tctctgtgcc agcagtgaat gacaccaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag atgacaccaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1128

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1128

gccttgccag cccgctcaga tgacaccgtc tcctattcca cagatcacac aggtgctgga 60

gtctcccagt ccccaaggta caaagtcgca aagaggggac gggatgtagc tctcaggtgt 120

gattcaattt cgggtcatgt aaccctttat tggtaccgac agaccctggg gcagggctca 180

gaggttctga cttactccca gagtgatgct caacgagaca aatcagggcg gcccagtggt 240

cggttctctg cagagaggcc tgagagatcc gtctccactc tgaagatcca gcgcacagag 300

cagggggact cagctgtgta tctctgtgcc agcagtgaat gacaccgtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag atgacaccgt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1129

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1129

gccttgccag cccgctcaga tgacaccaga gtgtcttcca cagatcacac aggtgctgga 60

gtctcccagt ccccaaggta caaagtcgca aagaggggac gggatgtagc tctcaggtgt 120

gattcaattt cgggtcatgt aaccctttat tggtaccgac agaccctggg gcagggctca 180

gaggttctga cttactccca gagtgatgct caacgagaca aatcagggcg gcccagtggt 240

cggttctctg cagagaggcc tgagagatcc gtctccactc tgaagatcca gcgcacagag 300

cagggggact cagctgtgta tctctgtgcc agcagtgaat gacaccagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct atgacaccag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1130

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1130

gccttgccag cccgctcaga tgacaccgtt ccgaattcca cagatcacac aggtgctgga 60

gtctcccagt ccccaaggta caaagtcgca aagaggggac gggatgtagc tctcaggtgt 120

gattcaattt cgggtcatgt aaccctttat tggtaccgac agaccctggg gcagggctca 180

gaggttctga cttactccca gagtgatgct caacgagaca aatcagggcg gcccagtggt 240

cggttctctg cagagaggcc tgagagatcc gtctccactc tgaagatcca gcgcacagag 300

cagggggact cagctgtgta tctctgtgcc agcagtgaat gacaccgttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat atgacaccgt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1131

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1131

gccttgccag cccgctcaga tgacacccgt tacttttcca cagatcacac aggtgctgga 60

gtctcccagt ccccaaggta caaagtcgca aagaggggac gggatgtagc tctcaggtgt 120

gattcaattt cgggtcatgt aaccctttat tggtaccgac agaccctggg gcagggctca 180

gaggttctga cttactccca gagtgatgct caacgagaca aatcagggcg gcccagtggt 240

cggttctctg cagagaggcc tgagagatcc gtctccactc tgaagatcca gcgcacagag 300

cagggggact cagctgtgta tctctgtgcc agcagtgaat gacacccgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc atgacacccg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1132

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1132

gccttgccag cccgctcaga tgacacctag gagacttcca cagatcacac aggtgctgga 60

gtctcccagt ccccaaggta caaagtcgca aagaggggac gggatgtagc tctcaggtgt 120

gattcaattt cgggtcatgt aaccctttat tggtaccgac agaccctggg gcagggctca 180

gaggttctga cttactccca gagtgatgct caacgagaca aatcagggcg gcccagtggt 240

cggttctctg cagagaggcc tgagagatcc gtctccactc tgaagatcca gcgcacagag 300

cagggggact cagctgtgta tctctgtgcc agcagtgaat gacacctagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg atgacaccta ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1133

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1133

gccttgccag cccgctcaga tgacaccgtg tctacttcca cagatcacac aggtgctgga 60

gtctcccagt ccccaaggta caaagtcgca aagaggggac gggatgtagc tctcaggtgt 120

gattcaattt cgggtcatgt aaccctttat tggtaccgac agaccctggg gcagggctca 180

gaggttctga cttactccca gagtgatgct caacgagaca aatcagggcg gcccagtggt 240

cggttctctg cagagaggcc tgagagatcc gtctccactc tgaagatcca gcgcacagag 300

cagggggact cagctgtgta tctctgtgcc agcagtgaat gacaccgtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc atgacaccgt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1134

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1134

gccttgccag cccgctcaga tgacaccgta cagtgttcca cagatcacac aggtgctgga 60

gtctcccagt ccccaaggta caaagtcgca aagaggggac gggatgtagc tctcaggtgt 120

gattcaattt cgggtcatgt aaccctttat tggtaccgac agaccctggg gcagggctca 180

gaggttctga cttactccca gagtgatgct caacgagaca aatcagggcg gcccagtggt 240

cggttctctg cagagaggcc tgagagatcc gtctccactc tgaagatcca gcgcacagag 300

cagggggact cagctgtgta tctctgtgcc agcagtgaat gacaccgtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg atgacaccgt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1135

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1135

gccttgccag cccgctcaga tgacaccgga tcatcttcca cagatcacac aggtgctgga 60

gtctcccagt ccccaaggta caaagtcgca aagaggggac gggatgtagc tctcaggtgt 120

gattcaattt cgggtcatgt aaccctttat tggtaccgac agaccctggg gcagggctca 180

gaggttctga cttactccca gagtgatgct caacgagaca aatcagggcg gcccagtggt 240

cggttctctg cagagaggcc tgagagatcc gtctccactc tgaagatcca gcgcacagag 300

cagggggact cagctgtgta tctctgtgcc agcagtgaat gacaccggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg atgacaccgg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1136

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1136

gccttgccag cccgctcaga tgacacctat tggcgttcca cagatcacac aggtgctgga 60

gtctcccagt ccccaaggta caaagtcgca aagaggggac gggatgtagc tctcaggtgt 120

gattcaattt cgggtcatgt aaccctttat tggtaccgac agaccctggg gcagggctca 180

gaggttctga cttactccca gagtgatgct caacgagaca aatcagggcg gcccagtggt 240

cggttctctg cagagaggcc tgagagatcc gtctccactc tgaagatcca gcgcacagag 300

cagggggact cagctgtgta tctctgtgcc agcagtgaat gacacctatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg atgacaccta ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1137

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1137

gccttgccag cccgctcaga tgacaccagg cttgattcca cagatcacac aggtgctgga 60

gtctcccagt ccccaaggta caaagtcgca aagaggggac gggatgtagc tctcaggtgt 120

gattcaattt cgggtcatgt aaccctttat tggtaccgac agaccctggg gcagggctca 180

gaggttctga cttactccca gagtgatgct caacgagaca aatcagggcg gcccagtggt 240

cggttctctg cagagaggcc tgagagatcc gtctccactc tgaagatcca gcgcacagag 300

cagggggact cagctgtgta tctctgtgcc agcagtgaat gacaccaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg atgacaccag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1138

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1138

gccttgccag cccgctcagc ttcacgagac actcttttcc acagatcaca caggtgctgg 60

agtctcccag tccccaaggt acgaagtcac acagagggga caggatgtag ctcccaggtg 120

tgatccaatt tcgggtcagg taacccttta ttggtaccga cagaccctgg ggcagggcca 180

agagtttctg acttccttcc aggatgaaac tcaacaagat aaatcagggc tgctcagtga 240

tcaattctcc acagagaggt ctgaggatct ttctccacct gaagatccag cgcacagagc 300

aagggcgact cggctgtgta tctctgtgcc agaagtgact tcacgagaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag cttcacgaga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1139

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1139

gccttgccag cccgctcagc ttcacgattc ggaactttcc acagatcaca caggtgctgg 60

agtctcccag tccccaaggt acgaagtcac acagagggga caggatgtag ctcccaggtg 120

tgatccaatt tcgggtcagg taacccttta ttggtaccga cagaccctgg ggcagggcca 180

agagtttctg acttccttcc aggatgaaac tcaacaagat aaatcagggc tgctcagtga 240

tcaattctcc acagagaggt ctgaggatct ttctccacct gaagatccag cgcacagagc 300

aagggcgact cggctgtgta tctctgtgcc agaagtgact tcacgattcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat cttcacgatt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1140

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1140

gccttgccag cccgctcagc ttcacgaaag taacgtttcc acagatcaca caggtgctgg 60

agtctcccag tccccaaggt acgaagtcac acagagggga caggatgtag ctcccaggtg 120

tgatccaatt tcgggtcagg taacccttta ttggtaccga cagaccctgg ggcagggcca 180

agagtttctg acttccttcc aggatgaaac tcaacaagat aaatcagggc tgctcagtga 240

tcaattctcc acagagaggt ctgaggatct ttctccacct gaagatccag cgcacagagc 300

aagggcgact cggctgtgta tctctgtgcc agaagtgact tcacgaaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag cttcacgaaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1141

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1141

gccttgccag cccgctcagc ttcacgagtc tcctatttcc acagatcaca caggtgctgg 60

agtctcccag tccccaaggt acgaagtcac acagagggga caggatgtag ctcccaggtg 120

tgatccaatt tcgggtcagg taacccttta ttggtaccga cagaccctgg ggcagggcca 180

agagtttctg acttccttcc aggatgaaac tcaacaagat aaatcagggc tgctcagtga 240

tcaattctcc acagagaggt ctgaggatct ttctccacct gaagatccag cgcacagagc 300

aagggcgact cggctgtgta tctctgtgcc agaagtgact tcacgagtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag cttcacgagt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1142

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1142

gccttgccag cccgctcagc ttcacgaaga gtgtctttcc acagatcaca caggtgctgg 60

agtctcccag tccccaaggt acgaagtcac acagagggga caggatgtag ctcccaggtg 120

tgatccaatt tcgggtcagg taacccttta ttggtaccga cagaccctgg ggcagggcca 180

agagtttctg acttccttcc aggatgaaac tcaacaagat aaatcagggc tgctcagtga 240

tcaattctcc acagagaggt ctgaggatct ttctccacct gaagatccag cgcacagagc 300

aagggcgact cggctgtgta tctctgtgcc agaagtgact tcacgaagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct cttcacgaag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1143

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1143

gccttgccag cccgctcagc ttcacgagtt ccgaatttcc acagatcaca caggtgctgg 60

agtctcccag tccccaaggt acgaagtcac acagagggga caggatgtag ctcccaggtg 120

tgatccaatt tcgggtcagg taacccttta ttggtaccga cagaccctgg ggcagggcca 180

agagtttctg acttccttcc aggatgaaac tcaacaagat aaatcagggc tgctcagtga 240

tcaattctcc acagagaggt ctgaggatct ttctccacct gaagatccag cgcacagagc 300

aagggcgact cggctgtgta tctctgtgcc agaagtgact tcacgagttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat cttcacgagt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1144

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1144

gccttgccag cccgctcagc ttcacgacgt tactttttcc acagatcaca caggtgctgg 60

agtctcccag tccccaaggt acgaagtcac acagagggga caggatgtag ctcccaggtg 120

tgatccaatt tcgggtcagg taacccttta ttggtaccga cagaccctgg ggcagggcca 180

agagtttctg acttccttcc aggatgaaac tcaacaagat aaatcagggc tgctcagtga 240

tcaattctcc acagagaggt ctgaggatct ttctccacct gaagatccag cgcacagagc 300

aagggcgact cggctgtgta tctctgtgcc agaagtgact tcacgacgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc cttcacgacg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1145

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1145

gccttgccag cccgctcagc ttcacgatag gagactttcc acagatcaca caggtgctgg 60

agtctcccag tccccaaggt acgaagtcac acagagggga caggatgtag ctcccaggtg 120

tgatccaatt tcgggtcagg taacccttta ttggtaccga cagaccctgg ggcagggcca 180

agagtttctg acttccttcc aggatgaaac tcaacaagat aaatcagggc tgctcagtga 240

tcaattctcc acagagaggt ctgaggatct ttctccacct gaagatccag cgcacagagc 300

aagggcgact cggctgtgta tctctgtgcc agaagtgact tcacgatagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg cttcacgata ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1146

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1146

gccttgccag cccgctcagc ttcacgagtg tctactttcc acagatcaca caggtgctgg 60

agtctcccag tccccaaggt acgaagtcac acagagggga caggatgtag ctcccaggtg 120

tgatccaatt tcgggtcagg taacccttta ttggtaccga cagaccctgg ggcagggcca 180

agagtttctg acttccttcc aggatgaaac tcaacaagat aaatcagggc tgctcagtga 240

tcaattctcc acagagaggt ctgaggatct ttctccacct gaagatccag cgcacagagc 300

aagggcgact cggctgtgta tctctgtgcc agaagtgact tcacgagtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc cttcacgagt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1147

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1147

gccttgccag cccgctcagc ttcacgagta cagtgtttcc acagatcaca caggtgctgg 60

agtctcccag tccccaaggt acgaagtcac acagagggga caggatgtag ctcccaggtg 120

tgatccaatt tcgggtcagg taacccttta ttggtaccga cagaccctgg ggcagggcca 180

agagtttctg acttccttcc aggatgaaac tcaacaagat aaatcagggc tgctcagtga 240

tcaattctcc acagagaggt ctgaggatct ttctccacct gaagatccag cgcacagagc 300

aagggcgact cggctgtgta tctctgtgcc agaagtgact tcacgagtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg cttcacgagt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1148

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1148

gccttgccag cccgctcagc ttcacgagga tcatctttcc acagatcaca caggtgctgg 60

agtctcccag tccccaaggt acgaagtcac acagagggga caggatgtag ctcccaggtg 120

tgatccaatt tcgggtcagg taacccttta ttggtaccga cagaccctgg ggcagggcca 180

agagtttctg acttccttcc aggatgaaac tcaacaagat aaatcagggc tgctcagtga 240

tcaattctcc acagagaggt ctgaggatct ttctccacct gaagatccag cgcacagagc 300

aagggcgact cggctgtgta tctctgtgcc agaagtgact tcacgaggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg cttcacgagg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1149

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1149

gccttgccag cccgctcagc ttcacgatat tggcgtttcc acagatcaca caggtgctgg 60

agtctcccag tccccaaggt acgaagtcac acagagggga caggatgtag ctcccaggtg 120

tgatccaatt tcgggtcagg taacccttta ttggtaccga cagaccctgg ggcagggcca 180

agagtttctg acttccttcc aggatgaaac tcaacaagat aaatcagggc tgctcagtga 240

tcaattctcc acagagaggt ctgaggatct ttctccacct gaagatccag cgcacagagc 300

aagggcgact cggctgtgta tctctgtgcc agaagtgact tcacgatatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg cttcacgata ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1150

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1150

gccttgccag cccgctcagc ttcacgaagg cttgatttcc acagatcaca caggtgctgg 60

agtctcccag tccccaaggt acgaagtcac acagagggga caggatgtag ctcccaggtg 120

tgatccaatt tcgggtcagg taacccttta ttggtaccga cagaccctgg ggcagggcca 180

agagtttctg acttccttcc aggatgaaac tcaacaagat aaatcagggc tgctcagtga 240

tcaattctcc acagagaggt ctgaggatct ttctccacct gaagatccag cgcacagagc 300

aagggcgact cggctgtgta tctctgtgcc agaagtgact tcacgaaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg cttcacgaag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1151

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1151

gccttgccag cccgctcaga gctagtggac actctttcca cagatcacac aggtgctgga 60

gtctcccagt ctcccaggta caaagtcaca aagaggggac aggatgtagc tctcaggtgt 120

gatccaattt cgggtcatgt atccctttat tggtaccgac aggccctggg gcagggccca 180

gagtttctga cttacttcaa ttatgaagcc caacaagaca aatcagggct gcccaatgat 240

cggttctctg cagagaggcc tgagggatcc atctccactc tgacgatcca gcgcacagag 300

cagcgggact cggccatgta tcgctgtgcc agcagtgaag ctagtggaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag agctagtgga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1152

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1152

gccttgccag cccgctcaga gctagtgttc ggaacttcca cagatcacac aggtgctgga 60

gtctcccagt ctcccaggta caaagtcaca aagaggggac aggatgtagc tctcaggtgt 120

gatccaattt cgggtcatgt atccctttat tggtaccgac aggccctggg gcagggccca 180

gagtttctga cttacttcaa ttatgaagcc caacaagaca aatcagggct gcccaatgat 240

cggttctctg cagagaggcc tgagggatcc atctccactc tgacgatcca gcgcacagag 300

cagcgggact cggccatgta tcgctgtgcc agcagtgaag ctagtgttcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat agctagtgtt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1153

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1153

gccttgccag cccgctcaga gctagtgaag taacgttcca cagatcacac aggtgctgga 60

gtctcccagt ctcccaggta caaagtcaca aagaggggac aggatgtagc tctcaggtgt 120

gatccaattt cgggtcatgt atccctttat tggtaccgac aggccctggg gcagggccca 180

gagtttctga cttacttcaa ttatgaagcc caacaagaca aatcagggct gcccaatgat 240

cggttctctg cagagaggcc tgagggatcc atctccactc tgacgatcca gcgcacagag 300

cagcgggact cggccatgta tcgctgtgcc agcagtgaag ctagtgaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag agctagtgaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1154

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1154

gccttgccag cccgctcaga gctagtggtc tcctattcca cagatcacac aggtgctgga 60

gtctcccagt ctcccaggta caaagtcaca aagaggggac aggatgtagc tctcaggtgt 120

gatccaattt cgggtcatgt atccctttat tggtaccgac aggccctggg gcagggccca 180

gagtttctga cttacttcaa ttatgaagcc caacaagaca aatcagggct gcccaatgat 240

cggttctctg cagagaggcc tgagggatcc atctccactc tgacgatcca gcgcacagag 300

cagcgggact cggccatgta tcgctgtgcc agcagtgaag ctagtggtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag agctagtggt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1155

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1155

gccttgccag cccgctcaga gctagtgaga gtgtcttcca cagatcacac aggtgctgga 60

gtctcccagt ctcccaggta caaagtcaca aagaggggac aggatgtagc tctcaggtgt 120

gatccaattt cgggtcatgt atccctttat tggtaccgac aggccctggg gcagggccca 180

gagtttctga cttacttcaa ttatgaagcc caacaagaca aatcagggct gcccaatgat 240

cggttctctg cagagaggcc tgagggatcc atctccactc tgacgatcca gcgcacagag 300

cagcgggact cggccatgta tcgctgtgcc agcagtgaag ctagtgagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct agctagtgag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1156

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1156

gccttgccag cccgctcaga gctagtggtt ccgaattcca cagatcacac aggtgctgga 60

gtctcccagt ctcccaggta caaagtcaca aagaggggac aggatgtagc tctcaggtgt 120

gatccaattt cgggtcatgt atccctttat tggtaccgac aggccctggg gcagggccca 180

gagtttctga cttacttcaa ttatgaagcc caacaagaca aatcagggct gcccaatgat 240

cggttctctg cagagaggcc tgagggatcc atctccactc tgacgatcca gcgcacagag 300

cagcgggact cggccatgta tcgctgtgcc agcagtgaag ctagtggttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat agctagtggt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1157

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1157

gccttgccag cccgctcaga gctagtgcgt tacttttcca cagatcacac aggtgctgga 60

gtctcccagt ctcccaggta caaagtcaca aagaggggac aggatgtagc tctcaggtgt 120

gatccaattt cgggtcatgt atccctttat tggtaccgac aggccctggg gcagggccca 180

gagtttctga cttacttcaa ttatgaagcc caacaagaca aatcagggct gcccaatgat 240

cggttctctg cagagaggcc tgagggatcc atctccactc tgacgatcca gcgcacagag 300

cagcgggact cggccatgta tcgctgtgcc agcagtgaag ctagtgcgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc agctagtgcg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1158

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1158

gccttgccag cccgctcaga gctagtgtag gagacttcca cagatcacac aggtgctgga 60

gtctcccagt ctcccaggta caaagtcaca aagaggggac aggatgtagc tctcaggtgt 120

gatccaattt cgggtcatgt atccctttat tggtaccgac aggccctggg gcagggccca 180

gagtttctga cttacttcaa ttatgaagcc caacaagaca aatcagggct gcccaatgat 240

cggttctctg cagagaggcc tgagggatcc atctccactc tgacgatcca gcgcacagag 300

cagcgggact cggccatgta tcgctgtgcc agcagtgaag ctagtgtagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg agctagtgta ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1159

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1159

gccttgccag cccgctcaga gctagtggtg tctacttcca cagatcacac aggtgctgga 60

gtctcccagt ctcccaggta caaagtcaca aagaggggac aggatgtagc tctcaggtgt 120

gatccaattt cgggtcatgt atccctttat tggtaccgac aggccctggg gcagggccca 180

gagtttctga cttacttcaa ttatgaagcc caacaagaca aatcagggct gcccaatgat 240

cggttctctg cagagaggcc tgagggatcc atctccactc tgacgatcca gcgcacagag 300

cagcgggact cggccatgta tcgctgtgcc agcagtgaag ctagtggtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc agctagtggt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1160

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1160

gccttgccag cccgctcaga gctagtggta cagtgttcca cagatcacac aggtgctgga 60

gtctcccagt ctcccaggta caaagtcaca aagaggggac aggatgtagc tctcaggtgt 120

gatccaattt cgggtcatgt atccctttat tggtaccgac aggccctggg gcagggccca 180

gagtttctga cttacttcaa ttatgaagcc caacaagaca aatcagggct gcccaatgat 240

cggttctctg cagagaggcc tgagggatcc atctccactc tgacgatcca gcgcacagag 300

cagcgggact cggccatgta tcgctgtgcc agcagtgaag ctagtggtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg agctagtggt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1161

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1161

gccttgccag cccgctcaga gctagtggga tcatcttcca cagatcacac aggtgctgga 60

gtctcccagt ctcccaggta caaagtcaca aagaggggac aggatgtagc tctcaggtgt 120

gatccaattt cgggtcatgt atccctttat tggtaccgac aggccctggg gcagggccca 180

gagtttctga cttacttcaa ttatgaagcc caacaagaca aatcagggct gcccaatgat 240

cggttctctg cagagaggcc tgagggatcc atctccactc tgacgatcca gcgcacagag 300

cagcgggact cggccatgta tcgctgtgcc agcagtgaag ctagtgggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg agctagtggg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1162

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1162

gccttgccag cccgctcaga gctagtgtat tggcgttcca cagatcacac aggtgctgga 60

gtctcccagt ctcccaggta caaagtcaca aagaggggac aggatgtagc tctcaggtgt 120

gatccaattt cgggtcatgt atccctttat tggtaccgac aggccctggg gcagggccca 180

gagtttctga cttacttcaa ttatgaagcc caacaagaca aatcagggct gcccaatgat 240

cggttctctg cagagaggcc tgagggatcc atctccactc tgacgatcca gcgcacagag 300

cagcgggact cggccatgta tcgctgtgcc agcagtgaag ctagtgtatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg agctagtgta ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1163

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1163

gccttgccag cccgctcaga gctagtgagg cttgattcca cagatcacac aggtgctgga 60

gtctcccagt ctcccaggta caaagtcaca aagaggggac aggatgtagc tctcaggtgt 120

gatccaattt cgggtcatgt atccctttat tggtaccgac aggccctggg gcagggccca 180

gagtttctga cttacttcaa ttatgaagcc caacaagaca aatcagggct gcccaatgat 240

cggttctctg cagagaggcc tgagggatcc atctccactc tgacgatcca gcgcacagag 300

cagcgggact cggccatgta tcgctgtgcc agcagtgaag ctagtgaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg agctagtgag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1164

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1164

gccttgccag cccgctcagt aaccaaggac actctttcca cagatcacac aggtgctgga 60

gtctcccagt ctcccaggta caaagtcaca aagaggggac aggatgtaac tctcaggtgt 120

gatccaattt cgagtcatgc aaccctttat tggtatcaac aggccctggg gcagggccca 180

gagtttctga cttacttcaa ttatgaagct caaccagaca aatcagggct gcccagtgat 240

cggttctctg cagagaggcc tgagggatcc atctccactc tgacgattca gcgcacagag 300

cagcgggact cagccatgta tcgctgtgcc agcagtgata accaaggaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag taaccaagga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1165

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1165

gccttgccag cccgctcagt aaccaagttc ggaacttcca cagatcacac aggtgctgga 60

gtctcccagt ctcccaggta caaagtcaca aagaggggac aggatgtaac tctcaggtgt 120

gatccaattt cgagtcatgc aaccctttat tggtatcaac aggccctggg gcagggccca 180

gagtttctga cttacttcaa ttatgaagct caaccagaca aatcagggct gcccagtgat 240

cggttctctg cagagaggcc tgagggatcc atctccactc tgacgattca gcgcacagag 300

cagcgggact cagccatgta tcgctgtgcc agcagtgata accaagttcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat taaccaagtt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1166

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1166

gccttgccag cccgctcagt aaccaagaag taacgttcca cagatcacac aggtgctgga 60

gtctcccagt ctcccaggta caaagtcaca aagaggggac aggatgtaac tctcaggtgt 120

gatccaattt cgagtcatgc aaccctttat tggtatcaac aggccctggg gcagggccca 180

gagtttctga cttacttcaa ttatgaagct caaccagaca aatcagggct gcccagtgat 240

cggttctctg cagagaggcc tgagggatcc atctccactc tgacgattca gcgcacagag 300

cagcgggact cagccatgta tcgctgtgcc agcagtgata accaagaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag taaccaagaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1167

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1167

gccttgccag cccgctcagt aaccaaggtc tcctattcca cagatcacac aggtgctgga 60

gtctcccagt ctcccaggta caaagtcaca aagaggggac aggatgtaac tctcaggtgt 120

gatccaattt cgagtcatgc aaccctttat tggtatcaac aggccctggg gcagggccca 180

gagtttctga cttacttcaa ttatgaagct caaccagaca aatcagggct gcccagtgat 240

cggttctctg cagagaggcc tgagggatcc atctccactc tgacgattca gcgcacagag 300

cagcgggact cagccatgta tcgctgtgcc agcagtgata accaaggtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag taaccaaggt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1168

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1168

gccttgccag cccgctcagt aaccaagaga gtgtcttcca cagatcacac aggtgctgga 60

gtctcccagt ctcccaggta caaagtcaca aagaggggac aggatgtaac tctcaggtgt 120

gatccaattt cgagtcatgc aaccctttat tggtatcaac aggccctggg gcagggccca 180

gagtttctga cttacttcaa ttatgaagct caaccagaca aatcagggct gcccagtgat 240

cggttctctg cagagaggcc tgagggatcc atctccactc tgacgattca gcgcacagag 300

cagcgggact cagccatgta tcgctgtgcc agcagtgata accaagagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct taaccaagag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1169

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1169

gccttgccag cccgctcagt aaccaaggtt ccgaattcca cagatcacac aggtgctgga 60

gtctcccagt ctcccaggta caaagtcaca aagaggggac aggatgtaac tctcaggtgt 120

gatccaattt cgagtcatgc aaccctttat tggtatcaac aggccctggg gcagggccca 180

gagtttctga cttacttcaa ttatgaagct caaccagaca aatcagggct gcccagtgat 240

cggttctctg cagagaggcc tgagggatcc atctccactc tgacgattca gcgcacagag 300

cagcgggact cagccatgta tcgctgtgcc agcagtgata accaaggttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat taaccaaggt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1170

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1170

gccttgccag cccgctcagt aaccaagcgt tacttttcca cagatcacac aggtgctgga 60

gtctcccagt ctcccaggta caaagtcaca aagaggggac aggatgtaac tctcaggtgt 120

gatccaattt cgagtcatgc aaccctttat tggtatcaac aggccctggg gcagggccca 180

gagtttctga cttacttcaa ttatgaagct caaccagaca aatcagggct gcccagtgat 240

cggttctctg cagagaggcc tgagggatcc atctccactc tgacgattca gcgcacagag 300

cagcgggact cagccatgta tcgctgtgcc agcagtgata accaagcgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc taaccaagcg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1171

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1171

gccttgccag cccgctcagt aaccaagtag gagacttcca cagatcacac aggtgctgga 60

gtctcccagt ctcccaggta caaagtcaca aagaggggac aggatgtaac tctcaggtgt 120

gatccaattt cgagtcatgc aaccctttat tggtatcaac aggccctggg gcagggccca 180

gagtttctga cttacttcaa ttatgaagct caaccagaca aatcagggct gcccagtgat 240

cggttctctg cagagaggcc tgagggatcc atctccactc tgacgattca gcgcacagag 300

cagcgggact cagccatgta tcgctgtgcc agcagtgata accaagtagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg taaccaagta ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1172

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1172

gccttgccag cccgctcagt aaccaaggtg tctacttcca cagatcacac aggtgctgga 60

gtctcccagt ctcccaggta caaagtcaca aagaggggac aggatgtaac tctcaggtgt 120

gatccaattt cgagtcatgc aaccctttat tggtatcaac aggccctggg gcagggccca 180

gagtttctga cttacttcaa ttatgaagct caaccagaca aatcagggct gcccagtgat 240

cggttctctg cagagaggcc tgagggatcc atctccactc tgacgattca gcgcacagag 300

cagcgggact cagccatgta tcgctgtgcc agcagtgata accaaggtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc taaccaaggt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1173

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1173

gccttgccag cccgctcagt aaccaaggta cagtgttcca cagatcacac aggtgctgga 60

gtctcccagt ctcccaggta caaagtcaca aagaggggac aggatgtaac tctcaggtgt 120

gatccaattt cgagtcatgc aaccctttat tggtatcaac aggccctggg gcagggccca 180

gagtttctga cttacttcaa ttatgaagct caaccagaca aatcagggct gcccagtgat 240

cggttctctg cagagaggcc tgagggatcc atctccactc tgacgattca gcgcacagag 300

cagcgggact cagccatgta tcgctgtgcc agcagtgata accaaggtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg taaccaaggt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1174

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1174

gccttgccag cccgctcagt aaccaaggga tcatcttcca cagatcacac aggtgctgga 60

gtctcccagt ctcccaggta caaagtcaca aagaggggac aggatgtaac tctcaggtgt 120

gatccaattt cgagtcatgc aaccctttat tggtatcaac aggccctggg gcagggccca 180

gagtttctga cttacttcaa ttatgaagct caaccagaca aatcagggct gcccagtgat 240

cggttctctg cagagaggcc tgagggatcc atctccactc tgacgattca gcgcacagag 300

cagcgggact cagccatgta tcgctgtgcc agcagtgata accaagggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg taaccaaggg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1175

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1175

gccttgccag cccgctcagt aaccaagtat tggcgttcca cagatcacac aggtgctgga 60

gtctcccagt ctcccaggta caaagtcaca aagaggggac aggatgtaac tctcaggtgt 120

gatccaattt cgagtcatgc aaccctttat tggtatcaac aggccctggg gcagggccca 180

gagtttctga cttacttcaa ttatgaagct caaccagaca aatcagggct gcccagtgat 240

cggttctctg cagagaggcc tgagggatcc atctccactc tgacgattca gcgcacagag 300

cagcgggact cagccatgta tcgctgtgcc agcagtgata accaagtatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg taaccaagta ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1176

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1176

gccttgccag cccgctcagt aaccaagagg cttgattcca cagatcacac aggtgctgga 60

gtctcccagt ctcccaggta caaagtcaca aagaggggac aggatgtaac tctcaggtgt 120

gatccaattt cgagtcatgc aaccctttat tggtatcaac aggccctggg gcagggccca 180

gagtttctga cttacttcaa ttatgaagct caaccagaca aatcagggct gcccagtgat 240

cggttctctg cagagaggcc tgagggatcc atctccactc tgacgattca gcgcacagag 300

cagcgggact cagccatgta tcgctgtgcc agcagtgata accaagaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg taaccaagag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1177

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1177

gccttgccag cccgctcagc aagaggtgac actctttcca cagatcacac aggtgctgga 60

gtctcccagt cccctaggta caaagtcgca aagagaggac aggatgtagc tctcaggtgt 120

gatccaattt cgggtcatgt atcccttttt tggtaccaac aggccctggg gcaggggcca 180

gagtttctga cttatttcca gaatgaagct caactagaca aatcggggct gcccagtgat 240

cgcttctttg cagaaaggcc tgagggatcc gtctccactc tgaagatcca gcgcacacag 300

caggaggact ccgccgtgta tctctgtgcc agcagtgaca agaggtgaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag caagaggtga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1178

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1178

gccttgccag cccgctcagc aagaggtttc ggaacttcca cagatcacac aggtgctgga 60

gtctcccagt cccctaggta caaagtcgca aagagaggac aggatgtagc tctcaggtgt 120

gatccaattt cgggtcatgt atcccttttt tggtaccaac aggccctggg gcaggggcca 180

gagtttctga cttatttcca gaatgaagct caactagaca aatcggggct gcccagtgat 240

cgcttctttg cagaaaggcc tgagggatcc gtctccactc tgaagatcca gcgcacacag 300

caggaggact ccgccgtgta tctctgtgcc agcagtgaca agaggtttcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat caagaggttt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1179

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1179

gccttgccag cccgctcagc aagaggtaag taacgttcca cagatcacac aggtgctgga 60

gtctcccagt cccctaggta caaagtcgca aagagaggac aggatgtagc tctcaggtgt 120

gatccaattt cgggtcatgt atcccttttt tggtaccaac aggccctggg gcaggggcca 180

gagtttctga cttatttcca gaatgaagct caactagaca aatcggggct gcccagtgat 240

cgcttctttg cagaaaggcc tgagggatcc gtctccactc tgaagatcca gcgcacacag 300

caggaggact ccgccgtgta tctctgtgcc agcagtgaca agaggtaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag caagaggtaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1180

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1180

gccttgccag cccgctcagc aagaggtgtc tcctattcca cagatcacac aggtgctgga 60

gtctcccagt cccctaggta caaagtcgca aagagaggac aggatgtagc tctcaggtgt 120

gatccaattt cgggtcatgt atcccttttt tggtaccaac aggccctggg gcaggggcca 180

gagtttctga cttatttcca gaatgaagct caactagaca aatcggggct gcccagtgat 240

cgcttctttg cagaaaggcc tgagggatcc gtctccactc tgaagatcca gcgcacacag 300

caggaggact ccgccgtgta tctctgtgcc agcagtgaca agaggtgtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag caagaggtgt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1181

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1181

gccttgccag cccgctcagc aagaggtaga gtgtcttcca cagatcacac aggtgctgga 60

gtctcccagt cccctaggta caaagtcgca aagagaggac aggatgtagc tctcaggtgt 120

gatccaattt cgggtcatgt atcccttttt tggtaccaac aggccctggg gcaggggcca 180

gagtttctga cttatttcca gaatgaagct caactagaca aatcggggct gcccagtgat 240

cgcttctttg cagaaaggcc tgagggatcc gtctccactc tgaagatcca gcgcacacag 300

caggaggact ccgccgtgta tctctgtgcc agcagtgaca agaggtagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct caagaggtag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1182

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1182

gccttgccag cccgctcagc aagaggtgtt ccgaattcca cagatcacac aggtgctgga 60

gtctcccagt cccctaggta caaagtcgca aagagaggac aggatgtagc tctcaggtgt 120

gatccaattt cgggtcatgt atcccttttt tggtaccaac aggccctggg gcaggggcca 180

gagtttctga cttatttcca gaatgaagct caactagaca aatcggggct gcccagtgat 240

cgcttctttg cagaaaggcc tgagggatcc gtctccactc tgaagatcca gcgcacacag 300

caggaggact ccgccgtgta tctctgtgcc agcagtgaca agaggtgttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat caagaggtgt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1183

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1183

gccttgccag cccgctcagc aagaggtcgt tacttttcca cagatcacac aggtgctgga 60

gtctcccagt cccctaggta caaagtcgca aagagaggac aggatgtagc tctcaggtgt 120

gatccaattt cgggtcatgt atcccttttt tggtaccaac aggccctggg gcaggggcca 180

gagtttctga cttatttcca gaatgaagct caactagaca aatcggggct gcccagtgat 240

cgcttctttg cagaaaggcc tgagggatcc gtctccactc tgaagatcca gcgcacacag 300

caggaggact ccgccgtgta tctctgtgcc agcagtgaca agaggtcgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc caagaggtcg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1184

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1184

gccttgccag cccgctcagc aagaggttag gagacttcca cagatcacac aggtgctgga 60

gtctcccagt cccctaggta caaagtcgca aagagaggac aggatgtagc tctcaggtgt 120

gatccaattt cgggtcatgt atcccttttt tggtaccaac aggccctggg gcaggggcca 180

gagtttctga cttatttcca gaatgaagct caactagaca aatcggggct gcccagtgat 240

cgcttctttg cagaaaggcc tgagggatcc gtctccactc tgaagatcca gcgcacacag 300

caggaggact ccgccgtgta tctctgtgcc agcagtgaca agaggttagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg caagaggtta ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1185

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1185

gccttgccag cccgctcagc aagaggtgtg tctacttcca cagatcacac aggtgctgga 60

gtctcccagt cccctaggta caaagtcgca aagagaggac aggatgtagc tctcaggtgt 120

gatccaattt cgggtcatgt atcccttttt tggtaccaac aggccctggg gcaggggcca 180

gagtttctga cttatttcca gaatgaagct caactagaca aatcggggct gcccagtgat 240

cgcttctttg cagaaaggcc tgagggatcc gtctccactc tgaagatcca gcgcacacag 300

caggaggact ccgccgtgta tctctgtgcc agcagtgaca agaggtgtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc caagaggtgt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1186

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1186

gccttgccag cccgctcagc aagaggtgta cagtgttcca cagatcacac aggtgctgga 60

gtctcccagt cccctaggta caaagtcgca aagagaggac aggatgtagc tctcaggtgt 120

gatccaattt cgggtcatgt atcccttttt tggtaccaac aggccctggg gcaggggcca 180

gagtttctga cttatttcca gaatgaagct caactagaca aatcggggct gcccagtgat 240

cgcttctttg cagaaaggcc tgagggatcc gtctccactc tgaagatcca gcgcacacag 300

caggaggact ccgccgtgta tctctgtgcc agcagtgaca agaggtgtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg caagaggtgt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1187

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1187

gccttgccag cccgctcagc aagaggtgga tcatcttcca cagatcacac aggtgctgga 60

gtctcccagt cccctaggta caaagtcgca aagagaggac aggatgtagc tctcaggtgt 120

gatccaattt cgggtcatgt atcccttttt tggtaccaac aggccctggg gcaggggcca 180

gagtttctga cttatttcca gaatgaagct caactagaca aatcggggct gcccagtgat 240

cgcttctttg cagaaaggcc tgagggatcc gtctccactc tgaagatcca gcgcacacag 300

caggaggact ccgccgtgta tctctgtgcc agcagtgaca agaggtggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg caagaggtgg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1188

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1188

gccttgccag cccgctcagc aagaggttat tggcgttcca cagatcacac aggtgctgga 60

gtctcccagt cccctaggta caaagtcgca aagagaggac aggatgtagc tctcaggtgt 120

gatccaattt cgggtcatgt atcccttttt tggtaccaac aggccctggg gcaggggcca 180

gagtttctga cttatttcca gaatgaagct caactagaca aatcggggct gcccagtgat 240

cgcttctttg cagaaaggcc tgagggatcc gtctccactc tgaagatcca gcgcacacag 300

caggaggact ccgccgtgta tctctgtgcc agcagtgaca agaggttatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg caagaggtta ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1189

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1189

gccttgccag cccgctcagc aagaggtagg cttgattcca cagatcacac aggtgctgga 60

gtctcccagt cccctaggta caaagtcgca aagagaggac aggatgtagc tctcaggtgt 120

gatccaattt cgggtcatgt atcccttttt tggtaccaac aggccctggg gcaggggcca 180

gagtttctga cttatttcca gaatgaagct caactagaca aatcggggct gcccagtgat 240

cgcttctttg cagaaaggcc tgagggatcc gtctccactc tgaagatcca gcgcacacag 300

caggaggact ccgccgtgta tctctgtgcc agcagtgaca agaggtaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg caagaggtag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1190

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1190

gccttgccag cccgctcaga gaatcaggac actctctcca cagatcacgc agatactgga 60

gtctcccaga accccagaca caagatcaca aagaggggac agaatgtaac tttcaggtgt 120

gatccaattt ctgaacacaa ccgcctttat tggtaccgac agaccctggg gcagggccca 180

gagtttctga cttacttcca gaatgaagct caactagaaa aatcaaggct gctcagtgat 240

cggttctctg cagagaggcc taagggatct ttctccacct tggagatcca gcgcacagag 300

cagggggact cggccatgta tctctgtgcc agcagtgaag aatcaggaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag agaatcagga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1191

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1191

gccttgccag cccgctcaga gaatcagttc ggaacctcca cagatcacgc agatactgga 60

gtctcccaga accccagaca caagatcaca aagaggggac agaatgtaac tttcaggtgt 120

gatccaattt ctgaacacaa ccgcctttat tggtaccgac agaccctggg gcagggccca 180

gagtttctga cttacttcca gaatgaagct caactagaaa aatcaaggct gctcagtgat 240

cggttctctg cagagaggcc taagggatct ttctccacct tggagatcca gcgcacagag 300

cagggggact cggccatgta tctctgtgcc agcagtgaag aatcagttcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat agaatcagtt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1192

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1192

gccttgccag cccgctcaga gaatcagaag taacgctcca cagatcacgc agatactgga 60

gtctcccaga accccagaca caagatcaca aagaggggac agaatgtaac tttcaggtgt 120

gatccaattt ctgaacacaa ccgcctttat tggtaccgac agaccctggg gcagggccca 180

gagtttctga cttacttcca gaatgaagct caactagaaa aatcaaggct gctcagtgat 240

cggttctctg cagagaggcc taagggatct ttctccacct tggagatcca gcgcacagag 300

cagggggact cggccatgta tctctgtgcc agcagtgaag aatcagaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag agaatcagaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1193

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1193

gccttgccag cccgctcaga gaatcaggtc tcctactcca cagatcacgc agatactgga 60

gtctcccaga accccagaca caagatcaca aagaggggac agaatgtaac tttcaggtgt 120

gatccaattt ctgaacacaa ccgcctttat tggtaccgac agaccctggg gcagggccca 180

gagtttctga cttacttcca gaatgaagct caactagaaa aatcaaggct gctcagtgat 240

cggttctctg cagagaggcc taagggatct ttctccacct tggagatcca gcgcacagag 300

cagggggact cggccatgta tctctgtgcc agcagtgaag aatcaggtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag agaatcaggt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1194

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1194

gccttgccag cccgctcaga gaatcagaga gtgtcctcca cagatcacgc agatactgga 60

gtctcccaga accccagaca caagatcaca aagaggggac agaatgtaac tttcaggtgt 120

gatccaattt ctgaacacaa ccgcctttat tggtaccgac agaccctggg gcagggccca 180

gagtttctga cttacttcca gaatgaagct caactagaaa aatcaaggct gctcagtgat 240

cggttctctg cagagaggcc taagggatct ttctccacct tggagatcca gcgcacagag 300

cagggggact cggccatgta tctctgtgcc agcagtgaag aatcagagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct agaatcagag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1195

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1195

gccttgccag cccgctcaga gaatcaggtt ccgaactcca cagatcacgc agatactgga 60

gtctcccaga accccagaca caagatcaca aagaggggac agaatgtaac tttcaggtgt 120

gatccaattt ctgaacacaa ccgcctttat tggtaccgac agaccctggg gcagggccca 180

gagtttctga cttacttcca gaatgaagct caactagaaa aatcaaggct gctcagtgat 240

cggttctctg cagagaggcc taagggatct ttctccacct tggagatcca gcgcacagag 300

cagggggact cggccatgta tctctgtgcc agcagtgaag aatcaggttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat agaatcaggt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1196

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1196

gccttgccag cccgctcaga gaatcagcgt tacttctcca cagatcacgc agatactgga 60

gtctcccaga accccagaca caagatcaca aagaggggac agaatgtaac tttcaggtgt 120

gatccaattt ctgaacacaa ccgcctttat tggtaccgac agaccctggg gcagggccca 180

gagtttctga cttacttcca gaatgaagct caactagaaa aatcaaggct gctcagtgat 240

cggttctctg cagagaggcc taagggatct ttctccacct tggagatcca gcgcacagag 300

cagggggact cggccatgta tctctgtgcc agcagtgaag aatcagcgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc agaatcagcg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1197

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1197

gccttgccag cccgctcaga gaatcagtag gagacctcca cagatcacgc agatactgga 60

gtctcccaga accccagaca caagatcaca aagaggggac agaatgtaac tttcaggtgt 120

gatccaattt ctgaacacaa ccgcctttat tggtaccgac agaccctggg gcagggccca 180

gagtttctga cttacttcca gaatgaagct caactagaaa aatcaaggct gctcagtgat 240

cggttctctg cagagaggcc taagggatct ttctccacct tggagatcca gcgcacagag 300

cagggggact cggccatgta tctctgtgcc agcagtgaag aatcagtagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg agaatcagta ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1198

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1198

gccttgccag cccgctcaga gaatcaggtg tctacctcca cagatcacgc agatactgga 60

gtctcccaga accccagaca caagatcaca aagaggggac agaatgtaac tttcaggtgt 120

gatccaattt ctgaacacaa ccgcctttat tggtaccgac agaccctggg gcagggccca 180

gagtttctga cttacttcca gaatgaagct caactagaaa aatcaaggct gctcagtgat 240

cggttctctg cagagaggcc taagggatct ttctccacct tggagatcca gcgcacagag 300

cagggggact cggccatgta tctctgtgcc agcagtgaag aatcaggtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc agaatcaggt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1199

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1199

gccttgccag cccgctcaga gaatcaggta cagtgctcca cagatcacgc agatactgga 60

gtctcccaga accccagaca caagatcaca aagaggggac agaatgtaac tttcaggtgt 120

gatccaattt ctgaacacaa ccgcctttat tggtaccgac agaccctggg gcagggccca 180

gagtttctga cttacttcca gaatgaagct caactagaaa aatcaaggct gctcagtgat 240

cggttctctg cagagaggcc taagggatct ttctccacct tggagatcca gcgcacagag 300

cagggggact cggccatgta tctctgtgcc agcagtgaag aatcaggtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg agaatcaggt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1200

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1200

gccttgccag cccgctcaga gaatcaggga tcatcctcca cagatcacgc agatactgga 60

gtctcccaga accccagaca caagatcaca aagaggggac agaatgtaac tttcaggtgt 120

gatccaattt ctgaacacaa ccgcctttat tggtaccgac agaccctggg gcagggccca 180

gagtttctga cttacttcca gaatgaagct caactagaaa aatcaaggct gctcagtgat 240

cggttctctg cagagaggcc taagggatct ttctccacct tggagatcca gcgcacagag 300

cagggggact cggccatgta tctctgtgcc agcagtgaag aatcagggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg agaatcaggg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1201

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1201

gccttgccag cccgctcaga gaatcagtat tggcgctcca cagatcacgc agatactgga 60

gtctcccaga accccagaca caagatcaca aagaggggac agaatgtaac tttcaggtgt 120

gatccaattt ctgaacacaa ccgcctttat tggtaccgac agaccctggg gcagggccca 180

gagtttctga cttacttcca gaatgaagct caactagaaa aatcaaggct gctcagtgat 240

cggttctctg cagagaggcc taagggatct ttctccacct tggagatcca gcgcacagag 300

cagggggact cggccatgta tctctgtgcc agcagtgaag aatcagtatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg agaatcagta ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1202

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1202

gccttgccag cccgctcaga gaatcagagg cttgactcca cagatcacgc agatactgga 60

gtctcccaga accccagaca caagatcaca aagaggggac agaatgtaac tttcaggtgt 120

gatccaattt ctgaacacaa ccgcctttat tggtaccgac agaccctggg gcagggccca 180

gagtttctga cttacttcca gaatgaagct caactagaaa aatcaaggct gctcagtgat 240

cggttctctg cagagaggcc taagggatct ttctccacct tggagatcca gcgcacagag 300

cagggggact cggccatgta tctctgtgcc agcagtgaag aatcagaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg agaatcagag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1203

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1203

gccttgccag cccgctcaga gttgaccgac actctctgcc tgcctctccc acagccccca 60

tggaggcagg gatcagccag ataccaagat atcacagaca cacagggaaa aagatcatcc 120

tgaaatttgc tcagattagg aaccattatt cagtgttctg ttatcaataa gaccaagaat 180

aggggctgag gctgatccat tattcaggta gtattggcag catgaccaaa ggcggtgcca 240

aggaagggta caatgtctct ggaaacaaac tcaagcattt tccctcaacc ctggagtcta 300

ctagcaccag ccagacctct gtacctctgt ggcagtgaag ttgaccgaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag agttgaccga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1204

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1204

gccttgccag cccgctcaga gttgaccttc ggaacctgcc tgcctctccc acagccccca 60

tggaggcagg gatcagccag ataccaagat atcacagaca cacagggaaa aagatcatcc 120

tgaaatttgc tcagattagg aaccattatt cagtgttctg ttatcaataa gaccaagaat 180

aggggctgag gctgatccat tattcaggta gtattggcag catgaccaaa ggcggtgcca 240

aggaagggta caatgtctct ggaaacaaac tcaagcattt tccctcaacc ctggagtcta 300

ctagcaccag ccagacctct gtacctctgt ggcagtgaag ttgaccttcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat agttgacctt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1205

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1205

gccttgccag cccgctcaga gttgaccaag taacgctgcc tgcctctccc acagccccca 60

tggaggcagg gatcagccag ataccaagat atcacagaca cacagggaaa aagatcatcc 120

tgaaatttgc tcagattagg aaccattatt cagtgttctg ttatcaataa gaccaagaat 180

aggggctgag gctgatccat tattcaggta gtattggcag catgaccaaa ggcggtgcca 240

aggaagggta caatgtctct ggaaacaaac tcaagcattt tccctcaacc ctggagtcta 300

ctagcaccag ccagacctct gtacctctgt ggcagtgaag ttgaccaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag agttgaccaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1206

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1206

gccttgccag cccgctcaga gttgaccgtc tcctactgcc tgcctctccc acagccccca 60

tggaggcagg gatcagccag ataccaagat atcacagaca cacagggaaa aagatcatcc 120

tgaaatttgc tcagattagg aaccattatt cagtgttctg ttatcaataa gaccaagaat 180

aggggctgag gctgatccat tattcaggta gtattggcag catgaccaaa ggcggtgcca 240

aggaagggta caatgtctct ggaaacaaac tcaagcattt tccctcaacc ctggagtcta 300

ctagcaccag ccagacctct gtacctctgt ggcagtgaag ttgaccgtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag agttgaccgt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1207

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1207

gccttgccag cccgctcaga gttgaccaga gtgtcctgcc tgcctctccc acagccccca 60

tggaggcagg gatcagccag ataccaagat atcacagaca cacagggaaa aagatcatcc 120

tgaaatttgc tcagattagg aaccattatt cagtgttctg ttatcaataa gaccaagaat 180

aggggctgag gctgatccat tattcaggta gtattggcag catgaccaaa ggcggtgcca 240

aggaagggta caatgtctct ggaaacaaac tcaagcattt tccctcaacc ctggagtcta 300

ctagcaccag ccagacctct gtacctctgt ggcagtgaag ttgaccagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct agttgaccag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1208

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1208

gccttgccag cccgctcaga gttgaccgtt ccgaactgcc tgcctctccc acagccccca 60

tggaggcagg gatcagccag ataccaagat atcacagaca cacagggaaa aagatcatcc 120

tgaaatttgc tcagattagg aaccattatt cagtgttctg ttatcaataa gaccaagaat 180

aggggctgag gctgatccat tattcaggta gtattggcag catgaccaaa ggcggtgcca 240

aggaagggta caatgtctct ggaaacaaac tcaagcattt tccctcaacc ctggagtcta 300

ctagcaccag ccagacctct gtacctctgt ggcagtgaag ttgaccgttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat agttgaccgt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1209

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1209

gccttgccag cccgctcaga gttgacccgt tacttctgcc tgcctctccc acagccccca 60

tggaggcagg gatcagccag ataccaagat atcacagaca cacagggaaa aagatcatcc 120

tgaaatttgc tcagattagg aaccattatt cagtgttctg ttatcaataa gaccaagaat 180

aggggctgag gctgatccat tattcaggta gtattggcag catgaccaaa ggcggtgcca 240

aggaagggta caatgtctct ggaaacaaac tcaagcattt tccctcaacc ctggagtcta 300

ctagcaccag ccagacctct gtacctctgt ggcagtgaag ttgacccgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc agttgacccg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1210

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1210

gccttgccag cccgctcaga gttgacctag gagacctgcc tgcctctccc acagccccca 60

tggaggcagg gatcagccag ataccaagat atcacagaca cacagggaaa aagatcatcc 120

tgaaatttgc tcagattagg aaccattatt cagtgttctg ttatcaataa gaccaagaat 180

aggggctgag gctgatccat tattcaggta gtattggcag catgaccaaa ggcggtgcca 240

aggaagggta caatgtctct ggaaacaaac tcaagcattt tccctcaacc ctggagtcta 300

ctagcaccag ccagacctct gtacctctgt ggcagtgaag ttgacctagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg agttgaccta ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1211

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1211

gccttgccag cccgctcaga gttgaccgtg tctacctgcc tgcctctccc acagccccca 60

tggaggcagg gatcagccag ataccaagat atcacagaca cacagggaaa aagatcatcc 120

tgaaatttgc tcagattagg aaccattatt cagtgttctg ttatcaataa gaccaagaat 180

aggggctgag gctgatccat tattcaggta gtattggcag catgaccaaa ggcggtgcca 240

aggaagggta caatgtctct ggaaacaaac tcaagcattt tccctcaacc ctggagtcta 300

ctagcaccag ccagacctct gtacctctgt ggcagtgaag ttgaccgtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc agttgaccgt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1212

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1212

gccttgccag cccgctcaga gttgaccgta cagtgctgcc tgcctctccc acagccccca 60

tggaggcagg gatcagccag ataccaagat atcacagaca cacagggaaa aagatcatcc 120

tgaaatttgc tcagattagg aaccattatt cagtgttctg ttatcaataa gaccaagaat 180

aggggctgag gctgatccat tattcaggta gtattggcag catgaccaaa ggcggtgcca 240

aggaagggta caatgtctct ggaaacaaac tcaagcattt tccctcaacc ctggagtcta 300

ctagcaccag ccagacctct gtacctctgt ggcagtgaag ttgaccgtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg agttgaccgt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1213

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1213

gccttgccag cccgctcaga gttgaccgga tcatcctgcc tgcctctccc acagccccca 60

tggaggcagg gatcagccag ataccaagat atcacagaca cacagggaaa aagatcatcc 120

tgaaatttgc tcagattagg aaccattatt cagtgttctg ttatcaataa gaccaagaat 180

aggggctgag gctgatccat tattcaggta gtattggcag catgaccaaa ggcggtgcca 240

aggaagggta caatgtctct ggaaacaaac tcaagcattt tccctcaacc ctggagtcta 300

ctagcaccag ccagacctct gtacctctgt ggcagtgaag ttgaccggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg agttgaccgg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1214

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1214

gccttgccag cccgctcaga gttgacctat tggcgctgcc tgcctctccc acagccccca 60

tggaggcagg gatcagccag ataccaagat atcacagaca cacagggaaa aagatcatcc 120

tgaaatttgc tcagattagg aaccattatt cagtgttctg ttatcaataa gaccaagaat 180

aggggctgag gctgatccat tattcaggta gtattggcag catgaccaaa ggcggtgcca 240

aggaagggta caatgtctct ggaaacaaac tcaagcattt tccctcaacc ctggagtcta 300

ctagcaccag ccagacctct gtacctctgt ggcagtgaag ttgacctatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg agttgaccta ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1215

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1215

gccttgccag cccgctcaga gttgaccagg cttgactgcc tgcctctccc acagccccca 60

tggaggcagg gatcagccag ataccaagat atcacagaca cacagggaaa aagatcatcc 120

tgaaatttgc tcagattagg aaccattatt cagtgttctg ttatcaataa gaccaagaat 180

aggggctgag gctgatccat tattcaggta gtattggcag catgaccaaa ggcggtgcca 240

aggaagggta caatgtctct ggaaacaaac tcaagcattt tccctcaacc ctggagtcta 300

ctagcaccag ccagacctct gtacctctgt ggcagtgaag ttgaccaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg agttgaccag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1216

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1216

gccttgccag cccgctcagc gtacgtagac actctttcac tttctgtctg cctctccagc 60

aggctctatt gatgctggga tcacccagat gccaagatat cacattgtac agaagaaaga 120

gatgatcctg gaatgtgctc aggttaggaa cagtgttctg atatcgacag gacccaagac 180

gggggctgaa gcttatccac tattcaggca gtggtcacag caggaccaaa gttgatgtca 240

cagaggggta ctgtgtttct tgaaacaagc ttgagcattt ccccaatcct ggcatccacc 300

agcaccagcc agacctatct gtaccactgt ggcagtgacg tacgtagaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag cgtacgtaga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1217

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1217

gccttgccag cccgctcagc gtacgtattc ggaacttcac tttctgtctg cctctccagc 60

aggctctatt gatgctggga tcacccagat gccaagatat cacattgtac agaagaaaga 120

gatgatcctg gaatgtgctc aggttaggaa cagtgttctg atatcgacag gacccaagac 180

gggggctgaa gcttatccac tattcaggca gtggtcacag caggaccaaa gttgatgtca 240

cagaggggta ctgtgtttct tgaaacaagc ttgagcattt ccccaatcct ggcatccacc 300

agcaccagcc agacctatct gtaccactgt ggcagtgacg tacgtattcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat cgtacgtatt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1218

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1218

gccttgccag cccgctcagc gtacgtaaag taacgttcac tttctgtctg cctctccagc 60

aggctctatt gatgctggga tcacccagat gccaagatat cacattgtac agaagaaaga 120

gatgatcctg gaatgtgctc aggttaggaa cagtgttctg atatcgacag gacccaagac 180

gggggctgaa gcttatccac tattcaggca gtggtcacag caggaccaaa gttgatgtca 240

cagaggggta ctgtgtttct tgaaacaagc ttgagcattt ccccaatcct ggcatccacc 300

agcaccagcc agacctatct gtaccactgt ggcagtgacg tacgtaaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag cgtacgtaaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1219

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1219

gccttgccag cccgctcagc gtacgtagtc tcctattcac tttctgtctg cctctccagc 60

aggctctatt gatgctggga tcacccagat gccaagatat cacattgtac agaagaaaga 120

gatgatcctg gaatgtgctc aggttaggaa cagtgttctg atatcgacag gacccaagac 180

gggggctgaa gcttatccac tattcaggca gtggtcacag caggaccaaa gttgatgtca 240

cagaggggta ctgtgtttct tgaaacaagc ttgagcattt ccccaatcct ggcatccacc 300

agcaccagcc agacctatct gtaccactgt ggcagtgacg tacgtagtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag cgtacgtagt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1220

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1220

gccttgccag cccgctcagc gtacgtaaga gtgtcttcac tttctgtctg cctctccagc 60

aggctctatt gatgctggga tcacccagat gccaagatat cacattgtac agaagaaaga 120

gatgatcctg gaatgtgctc aggttaggaa cagtgttctg atatcgacag gacccaagac 180

gggggctgaa gcttatccac tattcaggca gtggtcacag caggaccaaa gttgatgtca 240

cagaggggta ctgtgtttct tgaaacaagc ttgagcattt ccccaatcct ggcatccacc 300

agcaccagcc agacctatct gtaccactgt ggcagtgacg tacgtaagag tgtcgtcgac 360

atcagcccca gcattttggt gatgggactc gactctccat cctaggtaag ttgcagaatc 420

agggtggtat ggccattgtc ccttgaaggc agagttctct cgtacgtaag agtgtcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1221

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1221

gccttgccag cccgctcagc gtacgtagtt ccgaattcac tttctgtctg cctctccagc 60

aggctctatt gatgctggga tcacccagat gccaagatat cacattgtac agaagaaaga 120

gatgatcctg gaatgtgctc aggttaggaa cagtgttctg atatcgacag gacccaagac 180

gggggctgaa gcttatccac tattcaggca gtggtcacag caggaccaaa gttgatgtca 240

cagaggggta ctgtgtttct tgaaacaagc ttgagcattt ccccaatcct ggcatccacc 300

agcaccagcc agacctatct gtaccactgt ggcagtgacg tacgtagttc cgaagtcgac 360

ataattcacc cctccacttt gggaacggga ccaggctcac tgtgacaggt atgggggctc 420

cactcttgac tcgggggtgc ctgggtttga ctgcaatgat cgtacgtagt tccgaactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1222

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1222

gccttgccag cccgctcagc gtacgtacgt tacttttcac tttctgtctg cctctccagc 60

aggctctatt gatgctggga tcacccagat gccaagatat cacattgtac agaagaaaga 120

gatgatcctg gaatgtgctc aggttaggaa cagtgttctg atatcgacag gacccaagac 180

gggggctgaa gcttatccac tattcaggca gtggtcacag caggaccaaa gttgatgtca 240

cagaggggta ctgtgtttct tgaaacaagc ttgagcattt ccccaatcct ggcatccacc 300

agcaccagcc agacctatct gtaccactgt ggcagtgacg tacgtacgtt acttgtcgac 360

acaatgagca gttcttcggg ccagggacac ggctcaccgt gctaggtaag aagggggctc 420

caggtgggag agagggtgag cagcccagcc tgcacgaccc cgtacgtacg ttacttctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1223

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1223

gccttgccag cccgctcagc gtacgtatag gagacttcac tttctgtctg cctctccagc 60

aggctctatt gatgctggga tcacccagat gccaagatat cacattgtac agaagaaaga 120

gatgatcctg gaatgtgctc aggttaggaa cagtgttctg atatcgacag gacccaagac 180

gggggctgaa gcttatccac tattcaggca gtggtcacag caggaccaaa gttgatgtca 240

cagaggggta ctgtgtttct tgaaacaagc ttgagcattt ccccaatcct ggcatccacc 300

agcaccagcc agacctatct gtaccactgt ggcagtgacg tacgtatagg agacgtcgac 360

accggggagc tgttttttgg agaaggctct aggctgaccg tactgggtaa ggaggcggtt 420

ggggctccgg agagctccga gagggcggga tgggcagagg cgtacgtata ggagacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1224

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1224

gccttgccag cccgctcagc gtacgtagtg tctacttcac tttctgtctg cctctccagc 60

aggctctatt gatgctggga tcacccagat gccaagatat cacattgtac agaagaaaga 120

gatgatcctg gaatgtgctc aggttaggaa cagtgttctg atatcgacag gacccaagac 180

gggggctgaa gcttatccac tattcaggca gtggtcacag caggaccaaa gttgatgtca 240

cagaggggta ctgtgtttct tgaaacaagc ttgagcattt ccccaatcct ggcatccacc 300

agcaccagcc agacctatct gtaccactgt ggcagtgacg tacgtagtgt ctacgtcgac 360

agatacgcag tattttggcc caggcacccg gctgacagtg ctcggtaagc gggggctccc 420

gctgaagccc cggaactggg gagggggcgc cccgggacgc cgtacgtagt gtctacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1225

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1225

gccttgccag cccgctcagc gtacgtagta cagtgttcac tttctgtctg cctctccagc 60

aggctctatt gatgctggga tcacccagat gccaagatat cacattgtac agaagaaaga 120

gatgatcctg gaatgtgctc aggttaggaa cagtgttctg atatcgacag gacccaagac 180

gggggctgaa gcttatccac tattcaggca gtggtcacag caggaccaaa gttgatgtca 240

cagaggggta ctgtgtttct tgaaacaagc ttgagcattt ccccaatcct ggcatccacc 300

agcaccagcc agacctatct gtaccactgt ggcagtgacg tacgtagtac agtggtcgac 360

aaaacattca gtacttcggc gccgggaccc ggctctcagt gctgggtaag ctggggccgc 420

cgggggaccg gggacgagac tgcgctcggg tttttgtgcg cgtacgtagt acagtgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1226

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1226

gccttgccag cccgctcagc gtacgtagga tcatcttcac tttctgtctg cctctccagc 60

aggctctatt gatgctggga tcacccagat gccaagatat cacattgtac agaagaaaga 120

gatgatcctg gaatgtgctc aggttaggaa cagtgttctg atatcgacag gacccaagac 180

gggggctgaa gcttatccac tattcaggca gtggtcacag caggaccaaa gttgatgtca 240

cagaggggta ctgtgtttct tgaaacaagc ttgagcattt ccccaatcct ggcatccacc 300

agcaccagcc agacctatct gtaccactgt ggcagtgacg tacgtaggat catcgtcgac 360

gagacccagt acttcgggcc aggcacgcgg ctcctggtgc tcggtgagcg cgggctgctg 420

gggcgcgggc gcgggcggct tgggtctggt ttttgcgggg cgtacgtagg atcatcctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1227

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1227

gccttgccag cccgctcagc gtacgtatat tggcgttcac tttctgtctg cctctccagc 60

aggctctatt gatgctggga tcacccagat gccaagatat cacattgtac agaagaaaga 120

gatgatcctg gaatgtgctc aggttaggaa cagtgttctg atatcgacag gacccaagac 180

gggggctgaa gcttatccac tattcaggca gtggtcacag caggaccaaa gttgatgtca 240

cagaggggta ctgtgtttct tgaaacaagc ttgagcattt ccccaatcct ggcatccacc 300

agcaccagcc agacctatct gtaccactgt ggcagtgacg tacgtatatt ggcggtcgac 360

gggccaacgt cctgactttc ggggccggca gcaggctgac cgtgctgggt gagttttcgc 420

gggaccaccc gggcggcggg attcaggtgg aaggcggcgg cgtacgtata ttggcgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1228

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1228

gccttgccag cccgctcagc gtacgtaagg cttgattcac tttctgtctg cctctccagc 60

aggctctatt gatgctggga tcacccagat gccaagatat cacattgtac agaagaaaga 120

gatgatcctg gaatgtgctc aggttaggaa cagtgttctg atatcgacag gacccaagac 180

gggggctgaa gcttatccac tattcaggca gtggtcacag caggaccaaa gttgatgtca 240

cagaggggta ctgtgtttct tgaaacaagc ttgagcattt ccccaatcct ggcatccacc 300

agcaccagcc agacctatct gtaccactgt ggcagtgacg tacgtaaggc ttgagtcgac 360

acgagcagta cttcgggccg ggcaccaggc tcacggtcac aggtgagatt cgggcgtctc 420

cccaccttcc agcccctcgg tccccggagt cggagggtgg cgtacgtaag gcttgactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1229

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1229

gccttgccag cccgctcagt cggtactgac actcttctga cacacaggcc cagtggattc 60

tggagtcaca caaaccccaa agcacctgat cacagcaact ggacagcgag tgacgctgag 120

atgctcccct aggtctggag acctctctgt gtactggtac caacagagcc tggaccaggg 180

cctccagttc ctcattcagt attataatgg agaagagaga gcaaaaggaa acattcttga 240

acgattctcc gcacaacagt tccctgactt gcactctgaa ctaaacctga gctctctgga 300

gctgggggac tcagctttgt atttctgtgc cagcatgatc ggtactgaca ctctgtcgac 360

actgaagctt tctttggaca aggcaccaga ctcacagttg taggtaagac atttttcagg 420

ttcttttgca gatccgtcac agggaaaagt gggtccacag tcggtactga cactctctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1230

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1230

gccttgccag cccgctcagt cggtactttc ggaactctga cacacaggcc cagtggattc 60

tggagtcaca caaaccccaa agcacctgat cacagcaact ggacagcgag tgacgctgag 120

atgctcccct aggtctggag acctctctgt gtactggtac caacagagcc tggaccaggg 180

cctccagttc ctcattcagt attataatgg agaagagaga gcaaaaggaa acattcttga 240

acgattctcc gcacaacagt tccctgactt gcactctgaa ctaaacctga gctctctgga 300

gctgggggac tcagctttgt atttctgtgc cagcatgatc ggtactttcg gaacgtcgac 360

tatggctaca ccttcggttc ggggaccagg ttaaccgttg taggtaaggc tgggggtctc 420

taggaggggt gcgatgaggg aggactctgt cctgggaaat tcggtacttt cggaacctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1231

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1231

gccttgccag cccgctcagt cggtactaag taacgtctga cacacaggcc cagtggattc 60

tggagtcaca caaaccccaa agcacctgat cacagcaact ggacagcgag tgacgctgag 120

atgctcccct aggtctggag acctctctgt gtactggtac caacagagcc tggaccaggg 180

cctccagttc ctcattcagt attataatgg agaagagaga gcaaaaggaa acattcttga 240

acgattctcc gcacaacagt tccctgactt gcactctgaa ctaaacctga gctctctgga 300

gctgggggac tcagctttgt atttctgtgc cagcatgatc ggtactaagt aacggtcgac 360

gaaacaccat atattttgga gagggaagtt ggctcactgt tgtaggtgag taagtcaagg 420

ctggacagct gggaacttgc aaaaaggggc tggaatccag tcggtactaa gtaacgctga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1232

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1232

gccttgccag cccgctcagt cggtactgtc tcctatctga cacacaggcc cagtggattc 60

tggagtcaca caaaccccaa agcacctgat cacagcaact ggacagcgag tgacgctgag 120

atgctcccct aggtctggag acctctctgt gtactggtac caacagagcc tggaccaggg 180

cctccagttc ctcattcagt attataatgg agaagagaga gcaaaaggaa acattcttga 240

acgattctcc gcacaacagt tccctgactt gcactctgaa ctaaacctga gctctctgga 300

gctgggggac tcagctttgt atttctgtgc cagcatgatc ggtactgtct cctagtcgac 360

aatgaaaaac tgttttttgg cagtggaacc cagctctctg tcttgggtat gtaaaagact 420

tctttcggga tagtgtatca taaggtcgga gttccaggag tcggtactgt ctcctactga 480

tggcgcgagg gaggc 495

<210> SEQ ID NO 1233

<211> LENGTH: 495

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Synthetic Template Oligonucleotide for TCRB Locus

<400> SEQUENCE: 1233

gccttgccag cccgctcagt cggtactaga gtgtctctga cacacaggcc cagtggattc 60

tggagtcaca caaaccccaa agcacctgat cacagcaact ggacagcgag tgacgctgag 120

atgctcccct